The Experts below are selected from a list of 2010 Experts worldwide ranked by ideXlab platform
Renato A. Mortara - One of the best experts on this subject based on the ideXlab platform.
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distribution of trypanosoma cruzi stage specific epitopes in cardiac muscle of Calomys callosus balb c mice and cultured cells infected with different infective forms
Acta Tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
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Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms
Acta tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
Noemi Nosomi Taniwaki - One of the best experts on this subject based on the ideXlab platform.
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distribution of trypanosoma cruzi stage specific epitopes in cardiac muscle of Calomys callosus balb c mice and cultured cells infected with different infective forms
Acta Tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
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Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms
Acta tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
Jaime Polop - One of the best experts on this subject based on the ideXlab platform.
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Environmental determinants of the small mammal assemblage in an agroecosystem of central Argentina: The role of Calomys musculinus
Mammalian Biology, 2010Co-Authors: Ivana Simone, Francesca Cagnacci, Cecilia Provensal, Jaime PolopAbstract:Agricultural intensification in the central region of Argentina has been accompanied by rodent assemblage changes, involving an increase in Calomys species densities. In particular, Calomys musculinus , the main reservoir of Junín virus (etiological agent of the Argentine Haemorrhagic Fever, AHF) selects field borders over crop-fields. Borders represent relatively-stable habitats within agroecosystems, otherwise highly human modified landscapes. In this article, we assessed the effect of environmental variables on rodent species occurrence and assemblage in the field borders of a pampean agroecosystem. Small rodent occurrence was examined for variation according to vegetation species composition, structure and productivity, using canonical ordination techniques. In a total of 72 crop-field borders of Córdoba province, in spring, summer and autumn (2005-2006), we captured 1041 rodents of 8 different species. Akodon azarae, Calomys venustus and C. musculinus were the dominant species of the assemblage, with the first two associated one another, while C. musculinus tended to be related to different environmental variables. We showed that rodent species associations were mediated by resources that changed seasonally, such as vegetation cover. Also, the strength and “sign” of the association between species seemed to be a consequence of different habitat preferences and activity patterns. Finally, the intra-annual population cycle seemed to influence the relation between species and habitat structure and, possibly, inter-specific competition for resources. Therefore, we suggest that less generalist species of the rodent assemblage, well adapted to linear habitats, may limit C. musculinus occurrence throughout the agroecosystem by habitat and spatial competition. Any action aimed to control AHF, and therefore C. musculinus population density, should exclude negative effects on coexisting species.
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Combining geometric morphometrics and pattern recognition to identify interspecific patterns of skull variation: case study in sympatric Argentinian species of the genus Calomys (Rodentia: Cricetidae: Sigmodontinae)
Biological Journal of the Linnean Society, 2008Co-Authors: Pedro Cordeiro-estrela, Michel Baylac, Christiane Denys, Jaime PolopAbstract:Sympatric species of vesper mice Calomys laucha and Calomys musculinus are difficult to discriminate, especially in natural history collections where they are identified by external body measurements and cranial characteristics. Accurate identification of these two species can be important because only one of them, C. musculinus, is a Junin virus reservoir, the aetiological agent of the Argentine Hemorragic Fever. Research has focused into the development of molecular techniques to unambiguously identify these species. We apply statistical procedures from the field of pattern recognition to three-dimensional geometric morphometric data based on skull landmarks to identify sympatric species C. laucha, C. musculinus and Calomys venustus. Pattern recognition techniques correctly identified the three species without any prior information on specimen identity. By contrast to expectations, C. venustus differed from the other two species mainly on the basis of shape and not by its centroid size. The main sources of difference between C. laucha and C. musculinus were of shape, specifically localized at the landmarks defined by: (1) the sutures between the premaxillaries, the nasals and the frontals; (2) the sutures between the parietals, the frontals and the squamosals; and (3) the suture between the parietals and the interparietal. Nevertheless, allometries dominate the patterns of interspecific variation between these latter species and may partly explain past identification difficulties. Morphological evolution is discussed. The need for objective methods to define phenotypic clusters is highlighted with respect to the need for fast and precise biodiversity assessments. © 2008 The Linnean Society of London, Biological Journal of the Linnean Society, 2008, 94, 365–378.
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Juvenile dispersal in Calomys venustus (Muridae: Sigmodontinae)
Acta Oecologica, 2004Co-Authors: José Priotto, Cecilia Provensal, Andrea R. Steinmann, Jaime PolopAbstract:Both spacing behaviour and dispersal movement are viewed as hierarchical processes in which the effects may be expressed at spatial scale. This research was carried out to examine the hypothesis that the presence of parents promotes the dispersal of juveniles from their natal nest and their father or mother home-range, in Calomys venustus.The study was carried out in four 0.25 ha fences (two controls and two experimentals), in a natural pasture. This study had two periods: Father Removal (FR) (August and December 1997; year one) and Mother Removal (MR) (August 1998 and January 1999; year two). For the FR treatment fathers were removed after juveniles were born, but in the MR treatment mothers were removed after the juveniles were weaned. The effect of parents on the dispersal distance of juveniles was analysed with respect to their natal nest and their mother and father home-range. Dispersal distance from the nest of C. venustus was independent of either male or female parent. Juveniles were more dispersing in relation to the centre of activity of their mothers than to that of their fathers, and females were more dispersing than males. Female juveniles overlap their home-range with their parents less than male juveniles do. The differences observed between female and male juveniles would be related to their different sexual maturation times, as well as to the female territoriality.
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Effect of weather variables on the population fluctuation of muroid Calomys venustus in central Argentina
Acta Oecologica, 2002Co-Authors: Fabiana Castellarini, Cecilia Provensal, Jaime PolopAbstract:Abstract The effects of weather on population fluctuation patterns of the South American muroid Calomys venustus were studied. Box–Jenkins data series analysis and anova were used to describe the relationship between climatic variables and population. No relation was found between maximum temperatures or precipitation and the C . venustus population. Temperatures below 4 °C appear to affect populations of the overwintered cohort after a time lag of 5 months.
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Morphometric variation in populations of Calomys musculinus
Studies on Neotropical Fauna and Environment, 1993Co-Authors: María C. Provensal, Jaime PolopAbstract:Adult specimens of Calomys musculinus were selected by using discriminant functions. Variation in seven selected cranial mensural variables of these specimens from different localities, different habitats, and different years was examined using multivariate statistical analysis. In males, analysis of geographic trends indicated a relative character homogeneity between localities and a general correspondence between the samples of the same years. Significant inter‐annual variation within localities was revealed. In females there was no significant variation between localities and between yearly samples. It is suggested that the differences in the character states reflect differences in the course of development of the males in different years, possibly as the result of a demographically driven social selection pressure.
Claudio Vieira Da Silva - One of the best experts on this subject based on the ideXlab platform.
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distribution of trypanosoma cruzi stage specific epitopes in cardiac muscle of Calomys callosus balb c mice and cultured cells infected with different infective forms
Acta Tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
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Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms
Acta tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
Solange Da Silva - One of the best experts on this subject based on the ideXlab platform.
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distribution of trypanosoma cruzi stage specific epitopes in cardiac muscle of Calomys callosus balb c mice and cultured cells infected with different infective forms
Acta Tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
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Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms
Acta tropica, 2007Co-Authors: Noemi Nosomi Taniwaki, Claudio Vieira Da Silva, Solange Da Silva, Renato A. MortaraAbstract:Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK 2 cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypomastigotes (BT) from the Y strain of T. cruzi . Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites’ kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK 2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys , and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests.
