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Silva, Luciana Pereira - One of the best experts on this subject based on the ideXlab platform.
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Avaliação genotóxica de líquido de vesícula e extrato salino da forma metacestódea de Taenia solium e de agentes antiparasitários benzimidazólicos
Universidade Federal de Uberlândia, 2004Co-Authors: Silva, Luciana PereiraAbstract:A neurocisticercose (NCC) é a doença parasitária mais comum do sistema nervoso central (SNC) causada pela forma metacestódea de Taenia solium, a qual é prevalente em países em desenvolvimento e re-emergente em sociedades desenvolvidas. Esta parasitose tem sido associada com tumores cerebrais e câncer hematológico em humanos. O possível mecanismo de carcinogênese inclui: indução da imunossupressão pelo parasito; transferência do material genético entre parasito e hospedeiro; inflamação crônica; transformação de células pelos fatores secretados pela metacestódea de T. solium e produção de óxido nítrico e outras espécies reativas do oxigênio, com potencial genotóxico, causando danos no DNA, como aberrações cromossômicas. Além disso, os agentes benzimidazólicos utilizados no tratamento da NCC e de outras doenças parasitárias afetam a mitose, o número de cromossomos, a função do fuso e as estruturas dos microtúbulos. Os objetivos deste estudo foram avaliar a atividade genotóxica do líquido de vesícula (LV) e extrato salino (ES) da forma metacestódea de T. solium e de agentes benzimidazólicos [albendazol (ABZ), cambendazol (CBZ), mebendazol (MBZ) e tiabendazol (TBZ)] em asas de Drosophila melanogaster por meio do teste de mutação e recombinação somáticas (SMART). Larvas de terceiro estádio provenientes dos cruzamentos padrão (ST) e de alta bioativação (HB), foram tratadas, por aproximadamente 48 horas, com diferentes concentrações (12,5; 25,0 e 50,0 µg/mL) do LV e ES da forma metacestódea de T. solium; e (50; 75 e 100%) de ABZ, CBZ, MBZ e TBZ. Foram incluídos controles negativo (PBS e água destilada) e positivo (uretano 10 mM). Os resultados obtidos mostram que LV e ES foram genotóxicos em ambos cruzamentos do teste SMART, enquanto que nenhuma diferença estatisticamente significativa foi verificado nas freqüências de manchas induzidas pelos agentes benzimidazólicos, quando comparado com o controle negativo. Os experimentos sugerem que, nestas codições experimentais, o LV e ES da forma metacestódea de T. solium são genotóxicos, mas que os compostos ABZ, CBZ, MBZ e TBZ não são genotóxicos em células somáticas de D. melanogaster.Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by cysticerci of the helminth Taenia solium, which is prevalent in developing countries and re-emerging in affluent societies. This helminth has been associated with brain tumours and haematological malignancies in humans. The possible mechanisms leading to carcinogenesis include: parasite-induced immunosuppression; transfer of genetic material between parasite and host; chronic inflammation; cell transformation by factors secreted by T. solium metacestode and production of nitric oxide and other species reactive of oxygen with genotoxic potential causing either DNA damage as chromosome aberrations. In addition, the benzimidazoles agents using in the treatment of NCC and others parasitic diseases affect mitosis, chromosome number, spindle function and microtubule structure. The aim of this study was to analyze the genotoxicity of a saline extract (SE) and vesicular fluid (VF) of T. solium metacestodes and benzimidazoles agents [albendazole (ABZ), Cambendazole (CBZ), mebendazole (MBZ) and tiabendazole (TBZ)] in the Drosophila melanogaster wing somatic mutation and recombination test (SMART). Third-instar larvae derived from standard (ST) and high bioactivation (HB) crosses were treated for approximately 48 hours with different concentrations (12.5; 25.0 and 50.0 µg/mL) of SE and VF of T. solium metacestode and (50; 75 and 100%) of ABZ, CBZ, MBZ and TBZ. The results obtained shown that the VF and SE were genotoxic in both crosses and that no statistically significant differences in spot frequencies between controls and treated series of the benzimidazoles agents were observed. The experiments suggest that, under these experimental conditions, the VF and SE of T. solium metacestode is genotoxic, but the compounds ABZ, CBZ, MBZ and TBZ is not genotoxic in somatic cells of D. melanogaster
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Avaliação genotóxica de líquido de vesícula e extrato salino da forma metacestódea de Taenia solium e de agentes antiparasitários benzimidazólicos
'EDUFU - Editora da Universidade Federal de Uberlandia', 2004Co-Authors: Silva, Luciana PereiraAbstract:Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by cysticerci of the helminth Taenia solium, which is prevalent in developing countries and re-emerging in affluent societies. This helminth has been associated with brain tumours and haematological malignancies in humans. The possible mechanisms leading to carcinogenesis include: parasite-induced immunosuppression; transfer of genetic material between parasite and host; chronic inflammation; cell transformation by factors secreted by T. solium metacestode and production of nitric oxide and other species reactive of oxygen with genotoxic potential causing either DNA damage as chromosome aberrations. In addition, the benzimidazoles agents using in the treatment of NCC and others parasitic diseases affect mitosis, chromosome number, spindle function and microtubule structure. The aim of this study was to analyze the genotoxicity of a saline extract (SE) and vesicular fluid (VF) of T. solium metacestodes and benzimidazoles agents [albendazole (ABZ), Cambendazole (CBZ), mebendazole (MBZ) and tiabendazole (TBZ)] in the Drosophila melanogaster wing somatic mutation and recombination test (SMART). Third-instar larvae derived from standard (ST) and high bioactivation (HB) crosses were treated for approximately 48 hours with different concentrations (12.5; 25.0 and 50.0 µg/mL) of SE and VF of T. solium metacestode and (50; 75 and 100%) of ABZ, CBZ, MBZ and TBZ. The results obtained shown that the VF and SE were genotoxic in both crosses and that no statistically significant differences in spot frequencies between controls and treated series of the benzimidazoles agents were observed. The experiments suggest that, under these experimental conditions, the VF and SE of T. solium metacestode is genotoxic, but the compounds ABZ, CBZ, MBZ and TBZ is not genotoxic in somatic cells of D. melanogaster.Fundação de Amparo a Pesquisa do Estado de Minas GeraisDoutor em Imunologia e Parasitologia AplicadasA neurocisticercose (NCC) é a doença parasitária mais comum do sistema nervoso central (SNC) causada pela forma metacestódea de Taenia solium, a qual é prevalente em países em desenvolvimento e re-emergente em sociedades desenvolvidas. Esta parasitose tem sido associada com tumores cerebrais e câncer hematológico em humanos. O possível mecanismo de carcinogênese inclui: indução da imunossupressão pelo parasito; transferência do material genético entre parasito e hospedeiro; inflamação crônica; transformação de células pelos fatores secretados pela metacestódea de T. solium e produção de óxido nítrico e outras espécies reativas do oxigênio, com potencial genotóxico, causando danos no DNA, como aberrações cromossômicas. Além disso, os agentes benzimidazólicos utilizados no tratamento da NCC e de outras doenças parasitárias afetam a mitose, o número de cromossomos, a função do fuso e as estruturas dos microtúbulos. Os objetivos deste estudo foram avaliar a atividade genotóxica do líquido de vesícula (LV) e extrato salino (ES) da forma metacestódea de T. solium e de agentes benzimidazólicos [albendazol (ABZ), cambendazol (CBZ), mebendazol (MBZ) e tiabendazol (TBZ)] em asas de Drosophila melanogaster por meio do teste de mutação e recombinação somáticas (SMART). Larvas de terceiro estádio provenientes dos cruzamentos padrão (ST) e de alta bioativação (HB), foram tratadas, por aproximadamente 48 horas, com diferentes concentrações (12,5; 25,0 e 50,0 µg/mL) do LV e ES da forma metacestódea de T. solium; e (50; 75 e 100%) de ABZ, CBZ, MBZ e TBZ. Foram incluídos controles negativo (PBS e água destilada) e positivo (uretano 10 mM). Os resultados obtidos mostram que LV e ES foram genotóxicos em ambos cruzamentos do teste SMART, enquanto que nenhuma diferença estatisticamente significativa foi verificado nas freqüências de manchas induzidas pelos agentes benzimidazólicos, quando comparado com o controle negativo. Os experimentos sugerem que, nestas codições experimentais, o LV e ES da forma metacestódea de T. solium são genotóxicos, mas que os compostos ABZ, CBZ, MBZ e TBZ não são genotóxicos em células somáticas de D. melanogaster
David E. Granstrom - One of the best experts on this subject based on the ideXlab platform.
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control of Cambendazole resistant small strongyles population s with oxibendazole in a pony band an 8 year field test 1984 1992
Veterinary Parasitology, 1994Co-Authors: J H Drudge, S Stamper, David E. GranstromAbstract:Abstract Studies in a band of ponies harboring Population S benzimidazole-resistant small strongyles were initiated in 1974 and have continued for 18 years. Treatment (bimonthly) was with Cambendazole for the first 4 years and with oxibendazole (OBZ) for the next 14 years. Data on the first 10 years have been published. The present investigation includes the last 8 years (4 October 1984-11 September 1992), which are the seventh through fourteenth years, of treatment with OBZ. Pre- and posttreatment mean counts of strongyle eggs (epg) and larvae (lpg) per gram of feces were determined biweekly during the current study to monitor the efficacy of OBZ. The average annual percent reductions of epg counts effected by OBZ treatments were 51%, 53%, 38%, 38%, 39%, 28%, 40%, and 19% for the seventhth through fourteenth years, respectively. Similar levels of reductions were observed for lpg counts. Although OBZ was initially highly effective on this population of small strongyles, epg and lpg counts gradually declined, but have remained more or less constant since the fifth year of research. However, reductions of the counts were the lowest for the last year of the study.
J H Drudge - One of the best experts on this subject based on the ideXlab platform.
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control of Cambendazole resistant small strongyles population s with oxibendazole in a pony band an 8 year field test 1984 1992
Veterinary Parasitology, 1994Co-Authors: J H Drudge, S Stamper, David E. GranstromAbstract:Abstract Studies in a band of ponies harboring Population S benzimidazole-resistant small strongyles were initiated in 1974 and have continued for 18 years. Treatment (bimonthly) was with Cambendazole for the first 4 years and with oxibendazole (OBZ) for the next 14 years. Data on the first 10 years have been published. The present investigation includes the last 8 years (4 October 1984-11 September 1992), which are the seventh through fourteenth years, of treatment with OBZ. Pre- and posttreatment mean counts of strongyle eggs (epg) and larvae (lpg) per gram of feces were determined biweekly during the current study to monitor the efficacy of OBZ. The average annual percent reductions of epg counts effected by OBZ treatments were 51%, 53%, 38%, 38%, 39%, 28%, 40%, and 19% for the seventhth through fourteenth years, respectively. Similar levels of reductions were observed for lpg counts. Although OBZ was initially highly effective on this population of small strongyles, epg and lpg counts gradually declined, but have remained more or less constant since the fifth year of research. However, reductions of the counts were the lowest for the last year of the study.
S Stamper - One of the best experts on this subject based on the ideXlab platform.
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control of Cambendazole resistant small strongyles population s with oxibendazole in a pony band an 8 year field test 1984 1992
Veterinary Parasitology, 1994Co-Authors: J H Drudge, S Stamper, David E. GranstromAbstract:Abstract Studies in a band of ponies harboring Population S benzimidazole-resistant small strongyles were initiated in 1974 and have continued for 18 years. Treatment (bimonthly) was with Cambendazole for the first 4 years and with oxibendazole (OBZ) for the next 14 years. Data on the first 10 years have been published. The present investigation includes the last 8 years (4 October 1984-11 September 1992), which are the seventh through fourteenth years, of treatment with OBZ. Pre- and posttreatment mean counts of strongyle eggs (epg) and larvae (lpg) per gram of feces were determined biweekly during the current study to monitor the efficacy of OBZ. The average annual percent reductions of epg counts effected by OBZ treatments were 51%, 53%, 38%, 38%, 39%, 28%, 40%, and 19% for the seventhth through fourteenth years, respectively. Similar levels of reductions were observed for lpg counts. Although OBZ was initially highly effective on this population of small strongyles, epg and lpg counts gradually declined, but have remained more or less constant since the fifth year of research. However, reductions of the counts were the lowest for the last year of the study.
Aline C De Oliveira - One of the best experts on this subject based on the ideXlab platform.
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comparison of multivariate calibration methods to determine simultaneously mebendazole Cambendazole and mebendazole thiabendazole in pharmaceutical preparations by uv visible spectrophotometry
Journal of Pharmaceutical and Biomedical Analysis, 2003Co-Authors: Suely Rodrigues Cabeleira Andrade, Ieda Spacino Scarminio, Marlene Maria Fregonezi Nery, Aline C De OliveiraAbstract:Abstract Three multivariate calibration methods, Principal Component Regression (PCR), the K-matrix method and Q-mode factor analysis followed by varimax and Imbrie's oblique rotations were applied to the simultaneous spectrophotometric determinations of mebendazole (MBZ)–Cambendazole (CBZ) and thiabendazole (TBZ)–mebendazole in commercial samples of Exelmin and Helmiben. The calibration set concentrations were selected to contain a ±10% variation in the quantity of active ingredients as declared by the manufacturer. The Q-mode factor analysis provides superior results for the two pharmaceutical formulations. The K-matrix method proved to be totally inadequate for these determinations. Almost all Q-mode results have relative errors much smaller than 5% of the active ingredient contents. This investigation shows that PCR and Q-mode factor analysis can be used to determine MBZ–CBZ and TBZ–MBZ in commercial drugs.
