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Demet Cansaranduman - One of the best experts on this subject based on the ideXlab platform.

  • sponge derived natural bioactive glass microspheres with self assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications
    Chemical Engineering Journal, 2021
    Co-Authors: Murat Kaya, Ismail Bilican, Muhammad Mujtaba, Idris Sargin, Merve Erginer Haskoylu, Ebru Toksoy Oner, Kai Zheng, Aldo R Boccaccini, Demet Cansaranduman
    Abstract:

    Abstract Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres (diameter 209.4 ± 38.5 µm) were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels (approx. 3000) opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast Cell Culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed exCellent loading and release abilities for an anti-Cancer drug, carboplatin, in simulated solutions and in human Cancer Cell Culture, HeLa, through a real time Cell analyzer system. The drug loading capacity of the porous beads was determined as 10.59%. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres, which we name as giant macroporous silica (GMS), are promising materials for a range of applications including bone tissue engineering and drug delivery.

  • sponge derived natural bioactive glass microspheres with self assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications
    Chemical Engineering Journal, 2020
    Co-Authors: Murat Kaya, Ismail Bilican, Muhammad Mujtaba, Idris Sargin, Merve Erginer Haskoylu, Ebru Toksoy Oner, Kai Zheng, Aldo R Boccaccini, Demet Cansaranduman
    Abstract:

    Abstract Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast Cell Culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed exCellent loading and release abilities for an anti-Cancer drug, carboplatin, in simulated solutions and in human HeLa Cancer Cell Culture through a real time Cell analyzer system. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres are promising materials for a range of applications including bone tissue engineering and drug delivery.

Ismail Bilican - One of the best experts on this subject based on the ideXlab platform.

  • sponge derived natural bioactive glass microspheres with self assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications
    Chemical Engineering Journal, 2021
    Co-Authors: Murat Kaya, Ismail Bilican, Muhammad Mujtaba, Idris Sargin, Merve Erginer Haskoylu, Ebru Toksoy Oner, Kai Zheng, Aldo R Boccaccini, Demet Cansaranduman
    Abstract:

    Abstract Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres (diameter 209.4 ± 38.5 µm) were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels (approx. 3000) opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast Cell Culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed exCellent loading and release abilities for an anti-Cancer drug, carboplatin, in simulated solutions and in human Cancer Cell Culture, HeLa, through a real time Cell analyzer system. The drug loading capacity of the porous beads was determined as 10.59%. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres, which we name as giant macroporous silica (GMS), are promising materials for a range of applications including bone tissue engineering and drug delivery.

  • sponge derived natural bioactive glass microspheres with self assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications
    Chemical Engineering Journal, 2020
    Co-Authors: Murat Kaya, Ismail Bilican, Muhammad Mujtaba, Idris Sargin, Merve Erginer Haskoylu, Ebru Toksoy Oner, Kai Zheng, Aldo R Boccaccini, Demet Cansaranduman
    Abstract:

    Abstract Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast Cell Culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed exCellent loading and release abilities for an anti-Cancer drug, carboplatin, in simulated solutions and in human HeLa Cancer Cell Culture through a real time Cell analyzer system. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres are promising materials for a range of applications including bone tissue engineering and drug delivery.

Murat Kaya - One of the best experts on this subject based on the ideXlab platform.

  • sponge derived natural bioactive glass microspheres with self assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications
    Chemical Engineering Journal, 2021
    Co-Authors: Murat Kaya, Ismail Bilican, Muhammad Mujtaba, Idris Sargin, Merve Erginer Haskoylu, Ebru Toksoy Oner, Kai Zheng, Aldo R Boccaccini, Demet Cansaranduman
    Abstract:

    Abstract Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres (diameter 209.4 ± 38.5 µm) were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels (approx. 3000) opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast Cell Culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed exCellent loading and release abilities for an anti-Cancer drug, carboplatin, in simulated solutions and in human Cancer Cell Culture, HeLa, through a real time Cell analyzer system. The drug loading capacity of the porous beads was determined as 10.59%. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres, which we name as giant macroporous silica (GMS), are promising materials for a range of applications including bone tissue engineering and drug delivery.

  • sponge derived natural bioactive glass microspheres with self assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications
    Chemical Engineering Journal, 2020
    Co-Authors: Murat Kaya, Ismail Bilican, Muhammad Mujtaba, Idris Sargin, Merve Erginer Haskoylu, Ebru Toksoy Oner, Kai Zheng, Aldo R Boccaccini, Demet Cansaranduman
    Abstract:

    Abstract Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast Cell Culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed exCellent loading and release abilities for an anti-Cancer drug, carboplatin, in simulated solutions and in human HeLa Cancer Cell Culture through a real time Cell analyzer system. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres are promising materials for a range of applications including bone tissue engineering and drug delivery.

Daniel W Chan - One of the best experts on this subject based on the ideXlab platform.

  • fatty acid synthase fas expression in human breast Cancer Cell Culture supernatants and in breast Cancer patients
    Cancer Letters, 2001
    Co-Authors: Young Y Wang, Francis P Kuhajda, Ellen S Pizer, Wan Fang Han, Lori J Sokoll, Daniel W Chan
    Abstract:

    Fatty acid synthase (FAS) is selectively expressed in certain human Cancers, including carcinoma of the breast, prostate, colon, ovary, and endometrium, compared to normal human tissues and therefore is a putative tumor marker. In this study, we found FAS concentrations were elevated in Cell Culture supernatants during Cell growth in two human breast Cancer Cell lines but not other Cancer Cell lines. A quantitative enzyme-linked immunosorbent assay and Western blot analysis were employed in this study. In addition, serum FAS levels were significantly higher in breast Cancer patients with different clinical stages (Stage II: 0.59+/-0.09 units/l, Stage III: 0.79+/-0.13 units/l, and Stage IV: 1.39+/-0.35 units/l) compared with healthy subjects (0.27+/-0.02 units/l, P<0.05). Taken together, our data suggest that FAS expression may be a useful tumor marker for breast Cancer and play a role in assessing Cancer virulence.

Anders M Naar - One of the best experts on this subject based on the ideXlab platform.

  • a lipid free and insulin supplemented medium supports de novo fatty acid synthesis gene activation in melanoma Cells
    PLOS ONE, 2019
    Co-Authors: Su Wu, Anders M Naar
    Abstract:

    While investigating the role played by de novo lipid (DNL) biosynthesis in Cancer Cells, we sought a medium condition that would support Cell proliferation without providing any serum lipids. Here we report that a defined serum free Cell Culture medium condition containing insulin, transferrin and selenium (ITS) supports controlled study of transcriptional regulation of de novo fatty acid (DNFA) production and de novo cholesterol synthesis (DNCS) in melanoma Cell lines. This lipid-free ITS medium is able to support continuous proliferation of several melanoma Cell lines that utilize DNL to support their lipid requirements. We show that the ITS medium stimulates gene transcription in support of both DNFA and DNCS, specifically mediated by SREBP1/2 in melanoma Cells. We further found that the ITS medium promoted SREBP1 nuclear localization and occupancy on DNFA gene promoters. Our data show clear utility of this serum and lipid-free medium for melanoma Cancer Cell Culture and lipid-related areas of investigation.

  • a serum free and insulin supplemented Cell Culture medium ensures fatty acid synthesis gene activation in Cancer Cells
    bioRxiv, 2018
    Co-Authors: Anders M Naar
    Abstract:

    Abstract While investigating the role played by de novo fatty acid biosynthesis (DNFA) in Cancer Cells, we sought a medium condition that would support Cell proliferation without providing any serum lipids. Here we report that a defined serum free Cell Culture medium condition containing insulin, transferrin and selenium (ITS) supports controlled study of DNFA regulation in melanoma Cell lines. This lipid-free ITS medium is able to support proliferation of melanoma Cell lines that fulfill their lipid requirements via DNFA. We show that the ITS medium stimulates gene transcription in support of both DNFA and de novo cholesterol synthesis (DNCS), specifically mediated by SREBP1/2 in melanoma Cells. We further found that the ITS medium promoted SREBP1 nuclear localization and occupancy on DNFA gene promoters. Our data show clear utility of this serum and lipid-free medium for melanoma Cancer Cell Culture and lipid-related areas of investigation.