The Experts below are selected from a list of 219723 Experts worldwide ranked by ideXlab platform
Wu Fengjie - One of the best experts on this subject based on the ideXlab platform.
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Study of RNA interference targeting inhibition gene and protein expression of COX-2 in gastric Cancer Cell Line SGC7901
Journal of Binzhou Medical University, 2009Co-Authors: Wu FengjieAbstract:Objective To establish an efficient method of RNA interference on COX-2 of gastric Cancer Cell Line SGC7901,and provide a new pathway for gene therapy of gastric Cancer and its recurrence and metastasis.Methods Six pieces of COX-2 small interference RNA(siRNA) and one negative control siRNA were designed and synthesized chemically,gastric Cancer Cell Line SGC7901 were transfected with them mediated by three different transfection reagent.COX-2 mRNA and COX-2 protein of gastric Cancer Cell Line SGC7901 were checked with real time quantitative PCR and Western blotting methods respectively.Results Transfection of gastric Cancer Cell Line SGC7901 with report gene DNA(pcDNA/lacZ)could be mediated by TOPtranfection efficiently.Transfection rate of gastric Cancer Cell Line SGC7901 was 56.0%,cotransfection of gastric Cancer Cell Line SGC7901 with DNA(pcDNA/lacZ)and pcDNA/lacZ-siRNA could be mediated by TOPtranfection efficiently,siRNA brought about distinguished inhibition efficiency on DNA(pcDNA/lacZ).Compared to negative control,COX-2mRNA in gastric Cancer Cell Line SGC7901 measured by real time quantitative PCR after transfection with COX-2(0~5) siRNAs mediated by TOPtranfection were degraded significantly,COX-2 siRNA resulted in highest transfection efficiency 98.3%,others were above 90%.Protein expression of COX-2 in SGC7901 Cell Line measured by Western blotting was lower 71.5% after transfected with COX-2 siRNA.Conclusion Transfection of gastric Cancer Cell Line SGC7901 with COX-2 siRNA could be mediated by TOPtranfection efficiently,RNA interference on COX-2 mediated by TOPtranfection can inhibit gene and protein expression of COX-2 of gastric Cancer Cell Line SGC7901 efficiently,which may provide a new target and method for gene therapy of gastric Cancer and its recurrence and metastasis.
Su Ming-quan - One of the best experts on this subject based on the ideXlab platform.
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Study on the affinity of peptides K237 with human prostate Cancer Cell Line LNcap
Journal of Modern Oncology, 2007Co-Authors: Su Ming-quanAbstract:Objective: To investigate the affinity of peptides K237 with human prostate Cancer Cell Line LNcap. Methods: Immunohistochemical staining and reverse-transcriptase polymerase chain reaction(RT-PCR)were employed to detect the expression of receptor KDR;the binding ability of peptides K237 was determined by FACS and confocal microscope. Results: Human prostate Cancer Cell Line LNcap expressed KDR;flow cytometry showed that peptides K237 bind to LNcap in a concentration-dependent manner, the laser scanning confocal fluorescence microimaging system confirmed that peptides K237 was located in cytoplasm and Cell membrane. Conclusion:Peptides K237 have high affinity with human prostate Cancer Cell Line LNcap. We may use peptides K237 to inhibit the proliferation of prostate Cancer Cell through disrupting the autocrine pathway. These findings suggest a potential role of K237 as a novel therapeutic method for Cancer.
Jianye Zhang - One of the best experts on this subject based on the ideXlab platform.
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Up-regulates the expression of maspin gene in prostate Cancer Cell Line LNCaP
Acta pharmaceutica Sinica, 2006Co-Authors: Shi P, Yu Cx, Jiang Al, Xiao-yan Hu, Pengju Zhang, Jianye ZhangAbstract:To study the effect of curcumin on the apoptosis of prostate Cancer Cell Line LNCaP and regulation of expression of maspin gene. MTT and DNA electrophoresis were used to examine the Cell growth and apoptosis of prostate Cancer Cell Line LNCaP after treated with different doses of curcumin. The expression of maspin gene at transcription level and translation level was also detected by RT-PCR and Western blotting. pGL3-maspin luciferase expression vector, containing 847 bp (- 764 -/+ 83) DNA of maspin gene 5' promoter region, was transient transfected into LNCaP Cell. Through detecting the activity of luciferase, the effect of curcumin on the promoter of maspin was studied. Curcumin inhibited Cell growth, induced the apoptosis and enhanced the expression of maspin gene in LNCaP Cells. Curcumin up-regulated expression of maspin gene in LNCaP Cells through enhancing the transcription activity of promoter of maspin gene.
Xiao Qian - One of the best experts on this subject based on the ideXlab platform.
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Establishment of gastric Cancer Cell Line with stable over-expression of Cdx2
Guangdong Medical Journal, 2011Co-Authors: Xiao QianAbstract:Objective To establish a gastric Cancer Cell Line with stable over-expression of Cdx2 gene.Methods The recombined eukaryotic expression vector pCMV-Cdx2-HA or empty vector pCMV-HA was transfected into gastric Cancer MGC-803 Cells by Lipofectamine.G418-resistant colonies were selected by adding G418 to the medium.Cdx2 mRNA and protein expression was assessed by RT-PCR and Western blot.The Cell cycle progression and apoptosis were assessed by flow cytometry.Results A gastric Cancer Cell Line with stable over-expression of Cdx2 was established successfully,which was named MGC-803/Cdx2 Cells.Cdx2 mRNA and protein in MGC-803/Cdx2 Cells significantly increased,compared with those in MGC-803 Cells and MGC-803/EV Cells(P0.05).The proportion of Cells in G0/G1 phase and the apoptotic rate in MGC-803/Cdx2 Cells were significantly higher than those in MGC-803 Cells and MGC-803/EV Cells(P0.05).Conclusion Gastric Cancer Cell Line with stable over-expression of Cdx2,which induces Cell cycle arrest and Cell apoptosis,is established.
Shi P - One of the best experts on this subject based on the ideXlab platform.
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Up-regulates the expression of maspin gene in prostate Cancer Cell Line LNCaP
Acta pharmaceutica Sinica, 2006Co-Authors: Shi P, Yu Cx, Jiang Al, Xiao-yan Hu, Pengju Zhang, Jianye ZhangAbstract:To study the effect of curcumin on the apoptosis of prostate Cancer Cell Line LNCaP and regulation of expression of maspin gene. MTT and DNA electrophoresis were used to examine the Cell growth and apoptosis of prostate Cancer Cell Line LNCaP after treated with different doses of curcumin. The expression of maspin gene at transcription level and translation level was also detected by RT-PCR and Western blotting. pGL3-maspin luciferase expression vector, containing 847 bp (- 764 -/+ 83) DNA of maspin gene 5' promoter region, was transient transfected into LNCaP Cell. Through detecting the activity of luciferase, the effect of curcumin on the promoter of maspin was studied. Curcumin inhibited Cell growth, induced the apoptosis and enhanced the expression of maspin gene in LNCaP Cells. Curcumin up-regulated expression of maspin gene in LNCaP Cells through enhancing the transcription activity of promoter of maspin gene.
