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Hamzah Mohd Salleh - One of the best experts on this subject based on the ideXlab platform.
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characterization of low cost lipase by solid state fermentation of palm kernel cake using Candida Cylindracea
2016Co-Authors: Amal Ahmad Elgharbawy, Md Zahangir Alam, Hamzah Mohd SallehAbstract:This study aimed to identify the properties as well as the application of Candida Cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.08.0 respectively.Zn 2+,Mg 2+ and Ca 2+ stimulated the enzyme while Fe 3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity.Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol,ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production.
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esterification for butyl butyrate formation using Candida Cylindracea lipase produced from palm oil mill effluent supplemented medium
Arabian Journal of Chemistry, 2014Co-Authors: Md Zahangir Alam, Aliyu Salihu, Ismail M Abdulkarim, Hamzah Mohd SallehAbstract:Abstract The ability of Candida Cylindracea lipase produced using palm oil mill effluent (POME) as a basal medium to catalyze the esterification reaction for butyl butyrate formation was investigated. Butyric acid and n -butanol were used as substrates at different molar ratios. Different conversion yields were observed according to the affinity of the produced lipase toward the substrates. The n -butanol to butyric acid molar ratio of 8 and lipase concentration of 75 U/mg gave the highest butyl butyrate formation of 63.33% based on the statistical optimization using face centered central composite design (FCCCD) after 12 h reaction. The esterification potential of the POME based lipase when compared with the commercial lipase from the same strain using the optimum levels was found to show a similar pattern. It can be concluded therefore that the produced lipase possesses appropriate characteristics to be used as a biocatalyst in the esterification reactions for butyl butyrate formation.
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Statistical screening and optimization of factors that influence lipase production from palm kernel cake using Candida Cylindracea in solid-state bioconversion
2014Co-Authors: Amal Ahmad Elgharbawy, Zahangir Alam, Hamzah Mohd SallehAbstract:Although lipases are important class of industrial enzymes but the high cost of their production limits their applications. In this study, an abundant raw material in Malaysia, palm kernel cake (PKC), was investigated as a substrate ina fermentation process to produce lipase using solid-state bioconversion (SSB) with minimum requirements of machinery and external nutrients. This requires an optimization process in order to achieve maximum production with the minimum requirements. Optimization for the parameters that influence lipase production from Candida Cylindracea was conducted using statistical experimental designs including Plackett-Burman (PB) design, one-factor- at-a-time (OFAT) and response surface methodology (RSM). Optimization enhanced lipase production over 4-fold compared to the screening stage, in PKC media supplemented with 1.5% (w/w) yeast extract, 2.0% (v/w) Tween-80, 0.5% (v/w) olive oil and 7.0% (v/w) inoculum at pH 7.0 and 30oC within 72 hrs fermentation. Maximum activity of 400?2 U/g dry PKC was achieved. The analysis of variance (ANOVA) indicated that the model was significant (p
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statistical screening and optimization of factors that influence lipase production from palm kernel cake using Candida Cylindracea in solid state bioconversion
2014Co-Authors: Amal Ahmad Elgharbawy, Md Zahangir Alam, Hamzah Mohd SallehAbstract:Although lipases are important class of industrial enzymes but the high cost of their production limits their applications. In this study, an abundant raw material in Malaysia, palm kernel cake (PKC), was investigated as a substrate ina fermentation process to produce lipase using solid-state bioconversion (SSB) with minimum requirements of machinery and external nutrients. This requires an optimization process in order to achieve maximum production with the minimum requirements. Optimization for the parameters that influence lipase production from Candida Cylindracea was conducted using statistical experimental designs including Plackett-Burman (PB) design, one-factor- at-a-time (OFAT) and response surface methodology (RSM). Optimization enhanced lipase production over 4-fold compared to the screening stage, in PKC media supplemented with 1.5% (w/w) yeast extract, 2.0% (v/w) Tween-80, 0.5% (v/w) olive oil and 7.0% (v/w) inoculum at pH 7.0 and 30oC within 72 hrs fermentation. Maximum activity of 400?2 U/g dry PKC was achieved. The analysis of variance (ANOVA) indicated that the model was significant (p<0.05) with coefficient of determination (R2) 0.9893 which was very close to the adjusted R2 (0.9816) pointing the reliability of the model.
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kinetics of lipase production from Candida Cylindracea by solid state bioconversion of palm kernel cake
2014Co-Authors: Amal Ahmad Elgharbawy, Zahangir Alam, Hamzah Mohd SallehAbstract:Solid- state bioconversion (SSB) of palm kernel cake (PKC) by Candida Cylindracea is a cost-effective method to produce lipase from abundant raw material in Malaysia. This study was conducted to describe the growth profile of Candida Cylindracea on PKC using SSB. Since separating yeast cells (biomass) is not a feasible task, indirect method was used to monitor the growth during the fermentation process. This method was based on glucosamine, protein and carbohydrate content of the fermented media during the fermentation period. The results showed that the pH of the fermented PKC increased from 6.1 to 8.5 at the first 60 h and turned to neutral after that. Protein concentration of the fermented medium started to decrease within the first 24 h, and then increased gradually. Total carbohydrates decreased from 290 mg/g PKC to reach 155 mg/g PKC by the end of the fermentation. Glucosamine, a component in the mycelia cell walls, was measured to monitor the biomass during the fermentation. Maximum concentration of glucosamine achieved after 72 h and started to decrease to almost stable for the next hours. This data can be used to have a good understanding of the fermentation process and the lipase production.
Aliyu Salihu - One of the best experts on this subject based on the ideXlab platform.
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statistical optimization of ethanol based biodiesel production from sludge palm oil using locally produced Candida Cylindracea lipase
Waste and Biomass Valorization, 2016Co-Authors: Ricca Rahman Nasaruddin, Zahangir Alam, Mohammed Saedi Jami, Aliyu SalihuAbstract:Face centered central composite design was used to optimize the enzyme loading, reaction temperature and mixing speed of sludge palm oil (SPO) transesterification reaction using locally produced Candida Cylindracea lipase. The developed quadratic model was found to be significant at p < 0.05. The highest biodiesel yield of 57.5 % (w/w SPO) and 15.22 % free fatty acids (FFA) conversion to biodiesel with coefficient of determination R2 of 0.91 and 0.93, respectively were obtained at enzyme loading of 0.4 U/g SPO, 41 °C and 250 rpm mixing speed. Enzyme loading (U/g) gave little effect on the %FFA conversion to biodiesel and total biodiesel yield compared to temperature and mixing speed due to high FFA and water content, thus further studies are required for improvement. The study shows the low cost SPO and locally produced lipase have a promising potential in the utilization of waste for low cost biodiesel production.
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esterification for butyl butyrate formation using Candida Cylindracea lipase produced from palm oil mill effluent supplemented medium
Arabian Journal of Chemistry, 2014Co-Authors: Md Zahangir Alam, Aliyu Salihu, Ismail M Abdulkarim, Hamzah Mohd SallehAbstract:Abstract The ability of Candida Cylindracea lipase produced using palm oil mill effluent (POME) as a basal medium to catalyze the esterification reaction for butyl butyrate formation was investigated. Butyric acid and n -butanol were used as substrates at different molar ratios. Different conversion yields were observed according to the affinity of the produced lipase toward the substrates. The n -butanol to butyric acid molar ratio of 8 and lipase concentration of 75 U/mg gave the highest butyl butyrate formation of 63.33% based on the statistical optimization using face centered central composite design (FCCCD) after 12 h reaction. The esterification potential of the POME based lipase when compared with the commercial lipase from the same strain using the optimum levels was found to show a similar pattern. It can be concluded therefore that the produced lipase possesses appropriate characteristics to be used as a biocatalyst in the esterification reactions for butyl butyrate formation.
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characterization of Candida Cylindracea lipase produced from palm oil mill effluent based medium
2012Co-Authors: Aliyu Salihu, Zahangir Alam, Mohamed IsmailAbstract:Production of Candida Cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-Sepharose and Sephacryl S-200 exhibiting a single band with molecular mass of 58 kDa by SDS-PAGE and gel filtration. The temperature and pH optima of the enzyme were found to be 35 o C and 8, respectively. The enzyme was stable at pH 7.0 and 8.0 for 12 hr and also at temperature range of 25 o to 35 o C for 1 hr. Among the divalent cation salts tested on the lipase activity, Ca 2+ and Mn 2+ activated the enzyme most, while Cu 2+ and Fe 2+ inhibited it. Non-ionic (Tween-80 and Tween-20 and Triton X-100) and ionic detergents (SDS) at 1% appeared to have stimulatory and inhibitory effects on lipase activity respectively. In case of stability in organic solvents, water miscible solvents are concentration dependent; where their low concentrations showed some stimulatory effects, with marked inhibition at higher concentrations. It can be concluded that knowledge of these properties of the enzyme as described in this study could go a long way in its effective utilization for industrial application processes.
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purification and characterization of extracellular lipase from Candida Cylindracea using palm oil mill effluent supplemented medium
2011Co-Authors: Md Zahangir Alam, Aliyu Salihu, Mohamed Ismail Abdul Karim, Mohd Salleh HamzahAbstract:Production of Candida Cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of 58 kDa by SDS-PAGE and gel filtration. The temperature and pH optima of the enzyme were found to be 35oC and 8 respectively. The enzyme was stable at pH 7.0 and 8.0 for 12 hr and also at temperature range of 25 to 35oC for 1 hr. Among the divalent cation salts tested on the lipase activity, Ca2+ and Mn2+ activated the enzyme most, while Cu2+ and Fe2+ inhibited it. Non-ionic (Tween-80 and Tween-20 and Triton X-100) and ionic detergents (SDS) at 1% appeared to have stimulatory and inhibitory effects on lipase activity respectively. In case of stability in organic solvents, water miscible solvents are concentration dependent; where their low concentrations showed some stimulatory effects, with marked inhibition at higher concentrations. It can be concluded that knowledge of these properties of the enzyme as described in this study could go a long way in its effective utilization for industrial application processes.
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effect of process parameters on lipase production by Candida Cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
Journal of Molecular Catalysis B-enzymatic, 2011Co-Authors: Md Zahangir Alam, Aliyu Salihu, Ismail M Abdulkarim, Hamzah Mohd SallehAbstract:A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida Cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with the experimental data. All the three parameters were statistically significant at p < 0.05. The validation experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen demand (COD) removal throughout the fermentation period.
J Bello - One of the best experts on this subject based on the ideXlab platform.
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addition of lipase from Candida Cylindracea to a traditional formulation of a dry fermented sausage
Meat Science, 1996Co-Authors: Izaskun Zalacain, Iciar Astiasaran, Jose M Zapelena, J BelloAbstract:Abstract The objective of this work was to study the manufacture of sausage containing a traditional starter culture ( Lactobacillus plantarum and Staphylococcus carnosus ) together with an enzyme lipase from Candida Cylindracea as compared with that of a sausage with only starter. The acidity value showed more intense lipolysis in the sausage with lipase with this increase being especially important in the second week of drying. In spite of this, there was no significant ( p > 0.05) increase in the oxidative rancidity processes in this sausage. The analysis of short chain fatty acids suggested the enzyme and starter together produced a greater amount of such acids than did the enzyme or the starter separately. Almost all free fatty acids showed significantly higher values in the sausage with lipase with the exception of linolenic acid. The addition of enzyme lipase produced a higher proportion of free saturated acids and a lower proportion of polyunsaturated acids during the drying of the sausage.
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dry fermented sausages elaborated with lipase from Candida Cylindracea comparison with traditional formulations
Meat Science, 1995Co-Authors: Izaskun Zalacain, M J Zapelena, Iciar Astiasaran, J BelloAbstract:Abstract The addition of microbial lipase to fermented sausages was studied. A sausage with lipase from Candida Cylindracea and a control sausage with starter ( Lactobacillus plantarum and Staphylococcus carnosus ) were produced in a pilot plant. The acidity value and the amounts of the different free fatty acids (FFA) showed a higher intensity of lipolytic activity in sausages with lipase than in sausages with starter. In sausages with lipase, the percentage of saturated FFA was greater and that of polyunsaturated FFA was lower than in sausage with starter. Mono-unsaturated FFA percentage was similar in both sausages. TBA and peroxide values indicated that the increase of FFA produced by lipase action did not increase the rancidity. A slight increase in acetic, propionic and butyric acids was observed in sausage with lipase but this was not sufficient to develop excessive acidity in the product.
Md Zahangir Alam - One of the best experts on this subject based on the ideXlab platform.
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characterization of low cost lipase by solid state fermentation of palm kernel cake using Candida Cylindracea
2016Co-Authors: Amal Ahmad Elgharbawy, Md Zahangir Alam, Hamzah Mohd SallehAbstract:This study aimed to identify the properties as well as the application of Candida Cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.08.0 respectively.Zn 2+,Mg 2+ and Ca 2+ stimulated the enzyme while Fe 3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity.Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol,ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production.
-
esterification for butyl butyrate formation using Candida Cylindracea lipase produced from palm oil mill effluent supplemented medium
Arabian Journal of Chemistry, 2014Co-Authors: Md Zahangir Alam, Aliyu Salihu, Ismail M Abdulkarim, Hamzah Mohd SallehAbstract:Abstract The ability of Candida Cylindracea lipase produced using palm oil mill effluent (POME) as a basal medium to catalyze the esterification reaction for butyl butyrate formation was investigated. Butyric acid and n -butanol were used as substrates at different molar ratios. Different conversion yields were observed according to the affinity of the produced lipase toward the substrates. The n -butanol to butyric acid molar ratio of 8 and lipase concentration of 75 U/mg gave the highest butyl butyrate formation of 63.33% based on the statistical optimization using face centered central composite design (FCCCD) after 12 h reaction. The esterification potential of the POME based lipase when compared with the commercial lipase from the same strain using the optimum levels was found to show a similar pattern. It can be concluded therefore that the produced lipase possesses appropriate characteristics to be used as a biocatalyst in the esterification reactions for butyl butyrate formation.
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statistical screening and optimization of factors that influence lipase production from palm kernel cake using Candida Cylindracea in solid state bioconversion
2014Co-Authors: Amal Ahmad Elgharbawy, Md Zahangir Alam, Hamzah Mohd SallehAbstract:Although lipases are important class of industrial enzymes but the high cost of their production limits their applications. In this study, an abundant raw material in Malaysia, palm kernel cake (PKC), was investigated as a substrate ina fermentation process to produce lipase using solid-state bioconversion (SSB) with minimum requirements of machinery and external nutrients. This requires an optimization process in order to achieve maximum production with the minimum requirements. Optimization for the parameters that influence lipase production from Candida Cylindracea was conducted using statistical experimental designs including Plackett-Burman (PB) design, one-factor- at-a-time (OFAT) and response surface methodology (RSM). Optimization enhanced lipase production over 4-fold compared to the screening stage, in PKC media supplemented with 1.5% (w/w) yeast extract, 2.0% (v/w) Tween-80, 0.5% (v/w) olive oil and 7.0% (v/w) inoculum at pH 7.0 and 30oC within 72 hrs fermentation. Maximum activity of 400?2 U/g dry PKC was achieved. The analysis of variance (ANOVA) indicated that the model was significant (p<0.05) with coefficient of determination (R2) 0.9893 which was very close to the adjusted R2 (0.9816) pointing the reliability of the model.
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purification and characterization of extracellular lipase from Candida Cylindracea using palm oil mill effluent supplemented medium
2011Co-Authors: Md Zahangir Alam, Aliyu Salihu, Mohamed Ismail Abdul Karim, Mohd Salleh HamzahAbstract:Production of Candida Cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of 58 kDa by SDS-PAGE and gel filtration. The temperature and pH optima of the enzyme were found to be 35oC and 8 respectively. The enzyme was stable at pH 7.0 and 8.0 for 12 hr and also at temperature range of 25 to 35oC for 1 hr. Among the divalent cation salts tested on the lipase activity, Ca2+ and Mn2+ activated the enzyme most, while Cu2+ and Fe2+ inhibited it. Non-ionic (Tween-80 and Tween-20 and Triton X-100) and ionic detergents (SDS) at 1% appeared to have stimulatory and inhibitory effects on lipase activity respectively. In case of stability in organic solvents, water miscible solvents are concentration dependent; where their low concentrations showed some stimulatory effects, with marked inhibition at higher concentrations. It can be concluded that knowledge of these properties of the enzyme as described in this study could go a long way in its effective utilization for industrial application processes.
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effect of process parameters on lipase production by Candida Cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
Journal of Molecular Catalysis B-enzymatic, 2011Co-Authors: Md Zahangir Alam, Aliyu Salihu, Ismail M Abdulkarim, Hamzah Mohd SallehAbstract:A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida Cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with the experimental data. All the three parameters were statistically significant at p < 0.05. The validation experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen demand (COD) removal throughout the fermentation period.
Nenad M Kostic - One of the best experts on this subject based on the ideXlab platform.
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Enantioselective Aminolysis of an α-Chloroester Catalyzed by Candida Cylindracea Lipase Encapsulated in Sol−Gel Silica Glass
Organic Letters, 2001Co-Authors: Jovica D. Badjić, And Ekaterina N Kadnikova, Nenad M KosticAbstract:Lipase from Candida Cylindracea (CcL) encapsulated in porous silica glass by a sol−gel method catalyzes enantioselective aminolysis of ethyl 2-chloropropionate. A silica matrix enhances the enzyme activity, i.e., improves the yield. The scope and limitations of the aminolysis reaction were investigated, and dynamic kinetic resolution of the ester was achieved. Encapsulated lipase remains active when used repeatedly. Encapsulation much improves the chiral discrimination by lipase and makes this enzyme even more useful in organic chemistry.
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enantioselective aminolysis of an α chloroester catalyzed by Candida Cylindracea lipase encapsulated in sol gel silica glass
Organic Letters, 2001Co-Authors: Jovica D Badjic, And Ekaterina N Kadnikova, Nenad M KosticAbstract:Lipase from Candida Cylindracea (CcL) encapsulated in porous silica glass by a sol−gel method catalyzes enantioselective aminolysis of ethyl 2-chloropropionate. A silica matrix enhances the enzyme activity, i.e., improves the yield. The scope and limitations of the aminolysis reaction were investigated, and dynamic kinetic resolution of the ester was achieved. Encapsulated lipase remains active when used repeatedly. Encapsulation much improves the chiral discrimination by lipase and makes this enzyme even more useful in organic chemistry.
