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Sue Yee Lim - One of the best experts on this subject based on the ideXlab platform.
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The 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase.
Veterinary clinical pathology, 2020Co-Authors: Sue Yee Lim, Frédéric Carrière, Evangelia Stavroulaki, Panagiotis G. Xenoulis, Jan S. Suchodolski, Jonathan A. Lidbury, Jörg M. SteinerAbstract:Background The measurement of pancreatic lipase is important for the diagnosis of feline and Canine Pancreatitis. Recent studies have claimed that lipase assays using the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) as a substrate are more specific for measuring pancreatic lipase than traditional lipase assays. However, the analytical specificity of this assay for pancreatic lipase has not been demonstrated. Objectives We aimed to determine whether hepatic and/or lipoprotein lipases can interfere with the DGGR-based assay results in cats and dogs. We, therefore, compared plasma lipase activities measured using DGGR-based and pancreatic lipase immunoreactivity (PLI) assays before and after administering heparin, known to cause the release of hepatic and lipoprotein lipases, in cats and dogs. Methods Heparin was administered in six cats and six dogs. Blood was collected at baseline and 10, 20, 30, 60, and 120 minutes after heparin administration. Lipase activity was measured using a DGGR-based assay, and PLI concentrations were measured using the Spec fPL and cPL assays for cats and dogs, respectively. Results Plasma lipase activities, as measured using the DGGR-based assay, increased significantly 10 minutes after heparin administration in both cats (P = .003) and dogs (P = .006) and returned to baseline by 120 minutes. In contrast, PLI concentrations showed no significant changes after heparin administration. Conclusions DGGR is not only hydrolyzed by pancreatic lipase but also by hepatic lipase, lipoprotein lipase, or both, in cats and dogs. Since these extrapancreatic lipases are also naturally present in cats and dogs, they could contribute to the lack of analytical specificity for the DGGR-based assays.
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The 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase
Veterinary Clinical Pathology, 2020Co-Authors: Sue Yee Lim, Frédéric Carrière, Panagiotis Xenoulis, Evangelia Stavroulaki, Jonathan Lidbury, Jan Suchodolski, Jörg SteinerAbstract:Background: The measurement of pancreatic lipase is important for the diagnosis offeline and Canine Pancreatitis. Recent studies have claimed that lipase assays using the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6’-methylresorufin) ester (DGGR) as a substrate are more specific for measuring pancreatic lipase than traditional lipase assays. However, the analytical specificity of this assay for pancreatic lipase has not been demonstrated. Objectives: We aimed to determine whether hepatic and/or lipoprotein lipases can interfere with the DGGR-based assay results in cats and dogs. We, therefore, compared plasma lipase activities measured using DGGR-based and pancreatic lipase immunoreactivity (PLI) assays before and after administering heparin, known to cause the release of hepatic and lipoprotein lipases, in cats and dogs. Methods: Heparin was administered in six cats and six dogs. Blood was collected at baseline and 10, 20, 30, 60, and 120 minutes after heparin administration. Lipase activity was measured using a DGGR-based assay, and PLI concentrations were measured using the Spec fPL and cPL assays for cats and dogs, respectively. Results: Plasma lipase activities, as measured using the DGGR-based assay, increased significantly 10 minutes after heparin administration in both cats (P = .003) and dogs (P = .006) and returned to baseline by 120 minutes. In contrast, PLI concentrations showed no significant changes after heparin administration. Conclusions: DGGR is not only hydrolyzed by pancreatic lipase but also by hepatic lipase, lipoprotein lipase, or both, in cats and dogs. Since these extrapancreatic lipases are also naturally present in cats and dogs, they could contribute to the lack of analytical specificity for the DGGR-based assays.
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Quantitative contrast-enhanced ultrasonographic assessment of naturally occurring Pancreatitis in dogs.
Journal of veterinary internal medicine, 2014Co-Authors: Sue Yee Lim, K. Nakamura, K. Morishita, N. Sasaki, M. Murakami, T. Osuga, N. Yokoyama, H. Ohta, M. Yamasaki, M. TakiguchiAbstract:Background Quantitative contrast-enhanced ultrasonography (CEUS) can detect pancreatic perfusion changes in experimentally induced Canine Pancreatitis. However, its usefulness in detecting perfusion changes in naturally occurring Pancreatitis is unclear. Hypothesis/Objectives To determine the feasibility of using CEUS to detect pancreatic and duodenal perfusion changes in naturally occurring Canine Pancreatitis. Animals Twenty-three client-owned dogs with Pancreatitis, 12 healthy control dogs. Methods Dogs diagnosed with Pancreatitis were prospectively included. CEUS of the pancreas and duodenum were performed. Time-intensity curves were created from regions of interest in the pancreas and duodenum. Five perfusion parameters were obtained for statistical analyses: time to initial up-slope, peak time (Tp), time to wash-out (TTW), peak intensity (PI), and area under the curve (AUC). Results For the pancreas, Tp of the Pancreatitis group was prolonged when compared to controls (62 ± 11 seconds versus 39 ± 13 seconds; P
Jörg M. Steiner - One of the best experts on this subject based on the ideXlab platform.
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The 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase.
Veterinary clinical pathology, 2020Co-Authors: Sue Yee Lim, Frédéric Carrière, Evangelia Stavroulaki, Panagiotis G. Xenoulis, Jan S. Suchodolski, Jonathan A. Lidbury, Jörg M. SteinerAbstract:Background The measurement of pancreatic lipase is important for the diagnosis of feline and Canine Pancreatitis. Recent studies have claimed that lipase assays using the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) as a substrate are more specific for measuring pancreatic lipase than traditional lipase assays. However, the analytical specificity of this assay for pancreatic lipase has not been demonstrated. Objectives We aimed to determine whether hepatic and/or lipoprotein lipases can interfere with the DGGR-based assay results in cats and dogs. We, therefore, compared plasma lipase activities measured using DGGR-based and pancreatic lipase immunoreactivity (PLI) assays before and after administering heparin, known to cause the release of hepatic and lipoprotein lipases, in cats and dogs. Methods Heparin was administered in six cats and six dogs. Blood was collected at baseline and 10, 20, 30, 60, and 120 minutes after heparin administration. Lipase activity was measured using a DGGR-based assay, and PLI concentrations were measured using the Spec fPL and cPL assays for cats and dogs, respectively. Results Plasma lipase activities, as measured using the DGGR-based assay, increased significantly 10 minutes after heparin administration in both cats (P = .003) and dogs (P = .006) and returned to baseline by 120 minutes. In contrast, PLI concentrations showed no significant changes after heparin administration. Conclusions DGGR is not only hydrolyzed by pancreatic lipase but also by hepatic lipase, lipoprotein lipase, or both, in cats and dogs. Since these extrapancreatic lipases are also naturally present in cats and dogs, they could contribute to the lack of analytical specificity for the DGGR-based assays.
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Influence of feeding on serum Canine pancreatic lipase immunoreactivity concentrations.
Veterinary medicine (Auckland N.Z.), 2014Co-Authors: Jörg M. Steiner, Craig G. Ruaux, David A. WilliamsAbstract:Measurement of serum concentration of pancreatic lipase immunoreactivity (PLI) has been shown to be highly specific for exocrine pancreatic function and sensitive for the diagnosis of Canine Pancreatitis. Currently, it is recommended that food be withheld for at least 12 hours before collecting a blood sample for analysis from dogs. However, it is unknown whether feeding has any influence on serum Canine PLI concentration. Thus, the goal of this study was to evaluate the influence of feeding on serum Canine PLI concentrations in healthy dogs. Food was withheld from eight healthy adult Beagle dogs for at least 17 hours and a baseline serum sample (0 minutes) was collected. Dogs were fed and serum samples were collected at 15, 30, 45, 60, 75, 90, 105, 150, 180, 210, 240, 300, 360, 420, and 480 minutes. There was no significant difference in serum Canine PLI concentrations at any time after feeding ( P=0.131). We conclude that feeding has no significant influence on serum Canine PLI concentrations.
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Analytical validation of an ELISA for measurement of Canine pancreas-specific lipase.
Veterinary clinical pathology, 2010Co-Authors: Stacey Pazar Huth, Jörg M. Steiner, Roberta Relford, Marilyn I. Strong-townsend, David A. WilliamsAbstract:Background: The diagnosis of Canine Pancreatitis is challenging. Clinical presentation often includes nonspecific clinical signs, such as vomiting, anorexia, and abdominal discomfort. Increased serum lipase activity can be indicative of Pancreatitis; however, it can also be increased with other conditions. An immunoassay for measurement of Canine pancreas-specific lipase in Canine serum that would be suitable for commercial application and provide rapid results would be beneficial. Objective: The goal of this study was to validate the Spec cPL assay, a commercially available ELISA for the quantitative measurement of Canine pancreas-specific lipase. Methods: Dynamic range, dilutional linearity, precision, interfering substances, assay stability, and reproducibility were investigated for analytical validation. The method was compared with the reference assay, Canine pancreatic lipase immunoreactivity (cPLI), and included evaluation of a sample population of dogs and bias. Results: Analytical validation showed a dynamic range of 36–954 μg/L; good precision (intra- and interassay coefficient of variation
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Sensitivity of serum markers for Pancreatitis in dogs with macroscopic evidence of Pancreatitis
Veterinary therapeutics : research in applied veterinary medicine, 2008Co-Authors: Jörg M. Steiner, Shelley J. Newman, Panagiotis G. Xenoulis, Kristen Woosley, Jan S. Suchodolski, David A. Williams, Linda BartonAbstract:Pancreatitis is recognized as an important and common problem in dogs, but diagnosis can be challenging. Recently, new assays for the measurement of trypsin-a1-proteinase inhibitor complexes and Canine pancreatic lipase immunoreactivity (cPLI and Spec cPL) have been developed and analytically validated. This is the first report of a direct comparison of the sensitivity of these and other more traditional serum markers for the diagnosis of Canine Pancreatitis in a subset of dogs with this disease (i.e., dogs with both macroscopic and microscopic changes characteristic of Pancreatitis). Serum cPLI and Spec cPL concentrations showed the highest sensitivity for the diagnosis of Pancreatitis in this group of patients. Further studies will be required to compare the specificity of these serum markers and thus determine their overall clinical utility.
Tim Williams - One of the best experts on this subject based on the ideXlab platform.
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Validation of a commercial 1,2-o-dilauryl-rac-glycero glutaric acid-(6’-methylresorufin) ester lipase assay for diagnosis of Canine Pancreatitis
Veterinary record open, 2018Co-Authors: Emily L Goodband, Gonçalo Serrano, Fernando Constantino-casas, Joy Archer, Penelope Watson, Tim WilliamsAbstract:The objectives of this study were fourfold: technical validation of a commercial Canine 1,2-o-dilauryl-rac-glycero glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay, to calculate a reference interval for DGGR lipase by the indirect a posteriori method, to establish biological validity of the assay, and to assess agreement between DGGR lipase and specific Canine pancreatic lipase (Spec cPL) assays. Dogs with histologically confirmed acute Pancreatitis (n=3), chronic Pancreatitis (n=8) and normal pancreatic tissue (n=7) with stored (-80°C) serum samples were identified. Relevant controls were selected. Precision, reproducibility and linearity of DGGR lipase, and the effect of sample haemolysis and freezing, were assessed. Sensitivity and specificity of DGGR lipase and Spec cPL were determined. Agreement between these two parameters was calculated using Cohen's kappa coefficient (κ). The DGGR lipase assay demonstrated excellent precision, reproducibility and linearity. Sample haemolysis and storage at -80°C for 12 months did not influence the assay. DGGR lipase (>245IU/l) and Spec cPL (>400µg/l) both showed poor sensitivity but excellent specificity for acute Pancreatitis, and poor to moderate sensitivity but excellent specificity for chronic Pancreatitis. Substantial agreement (κ=0.679) was found between DGGR lipase and Spec cPL. The validated DGGR lipase assay had similar sensitivity and specificity for the diagnosis of acute and chronic Pancreatitis to Spec cPL. DGGR lipase is a reliable alternative to Spec cPL for the diagnosis of Pancreatitis.
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validation of a commercial 1 2 o dilauryl rac glycero glutaric acid 6 methylresorufin ester lipase assay for diagnosis of Canine Pancreatitis
Veterinary Record Open, 2018Co-Authors: Emily L Goodband, Gonçalo Serrano, Joy Archer, Penelope Watson, Fernando Constantinocasas, Tim WilliamsAbstract:The objectives of this study were fourfold: technical validation of a commercial Canine 1,2-o-dilauryl-rac-glycero glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay, to calculate a reference interval for DGGR lipase by the indirect a posteriori method, to establish biological validity of the assay, and to assess agreement between DGGR lipase and specific Canine pancreatic lipase (Spec cPL) assays. Dogs with histologically confirmed acute Pancreatitis (n=3), chronic Pancreatitis (n=8) and normal pancreatic tissue (n=7) with stored (-80°C) serum samples were identified. Relevant controls were selected. Precision, reproducibility and linearity of DGGR lipase, and the effect of sample haemolysis and freezing, were assessed. Sensitivity and specificity of DGGR lipase and Spec cPL were determined. Agreement between these two parameters was calculated using Cohen's kappa coefficient (κ). The DGGR lipase assay demonstrated excellent precision, reproducibility and linearity. Sample haemolysis and storage at -80°C for 12 months did not influence the assay. DGGR lipase (>245IU/l) and Spec cPL (>400µg/l) both showed poor sensitivity but excellent specificity for acute Pancreatitis, and poor to moderate sensitivity but excellent specificity for chronic Pancreatitis. Substantial agreement (κ=0.679) was found between DGGR lipase and Spec cPL. The validated DGGR lipase assay had similar sensitivity and specificity for the diagnosis of acute and chronic Pancreatitis to Spec cPL. DGGR lipase is a reliable alternative to Spec cPL for the diagnosis of Pancreatitis.
Richard A. Prinz - One of the best experts on this subject based on the ideXlab platform.
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The effect of interleukin-6 on bacterial translocation in acute Canine Pancreatitis
International Journal of Gastrointestinal Cancer, 2000Co-Authors: Qiang Liu, Goldie Djuricin, Catherine Nathan, Paolo Gattuso, Robert A. Weinstein, Richard A. PrinzAbstract:Background. Bacterial translocation from the gut to mesenteric lymph nodes and other extraintestinal sites is an important source of infection in acute Pancreatitis. Impaired host immunity is known to promote bacterial translocation. Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, acute phase reaction, and hematopoiesis. Methods. Twenty-four mongrel dogs (18–29 kg) were studied in four equal groups. In Groups I and II, acute Pancreatitis was induced by direct pressure injection of 4% taurocholate and trypsin into the pancreatic duct at laparotomy. Groups III and IV had only laparotomy. Group I and III dogs were given IL-6 (50 µg/kg/d, sq) daily starting 24 h after operation and Group II and IV dogs received an equal volume of saline administered at similar time. All animals had blood drawn for culture, complete blood count (CBC), platelets, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and amylase on d 0, 1, 4, and 7. On d 7, mesenteric lymph nodes (MLN), spleen, liver, pancreas, and cecum were harvested for pathology study and for cultures of aerobic and anaerobic bacteria. Quantitative cecal cultures of aerobic and anaerobic bacteria were obtained. Results. All Group I and Group II dogs had severe Pancreatitis. The increase of plasma CRP in Group I was sustained throughout treatment (1.3±0.3 on d 0 vs 3.1±0.3*, 3.0±0.3*, and 2.9±0.3* and d 1,4, and 7, respectively). Plasma CRP was increased in Group II on d 1 and d 4 (1.3±0.3 mg/dL on d 0 vs 3.6±0.3* mg/dL on d 1, and 3.1±0.3* on d 4, * p
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The effect of interleukin-6 on bacterial translocation in acute Canine Pancreatitis.
International journal of pancreatology : official journal of the International Association of Pancreatology, 2000Co-Authors: Qiang Liu, Goldie Djuricin, Catherine Nathan, Paolo Gattuso, Robert A. Weinstein, Richard A. PrinzAbstract:Background. Bacterial translocation from the gut to mesenteric lymph nodes and other extraintestinal sites is an important source of infection in acute Pancreatitis. Impaired host immunity is known to promote bacterial translocation. Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, acute phase reaction, and hematopoiesis.
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Effect of somatostatin analogue and cholecystokinin receptor antagonist on bile-induced acute Canine Pancreatitis.
The American surgeon, 1992Co-Authors: Martin Bertram, Richard A. Prinz, Melanie J. Castelli, G. Djuricin, H. K. JacobsAbstract:To determine whether a synthetic somatostatin analogue, octreotide, and a cholecystokinin receptor antagonist, L-364,718, may be beneficial in acute Pancreatitis, 33 dogs were assigned to four groups. Each dog underwent laparotomy with injection of autologous bile into the dorsal pancreatic duct. Thirty minutes after the induction of Pancreatitis, Group 1 received a subcutaneous injection of octreotide (200 micrograms/kg), Group 2 received an equal volume of the octreotide carrier, Group 3 received an hourly intravenous bolus of L-364,718 (60 micrograms/kg), and Group 4 received an equal volume of the L-364,718 carrier. Hemodynamic profiles, arterial blood gases, plasma glucose, and serum amylase were obtained before laparotomy, at bile injection, and at hourly intervals. The pancreas was removed after 8 hours for gross evaluation, measurement of water content, and histologic examination. A significant decrease in cardiac index and a significant increase in serum amylase and pancreatic edema occurred in all four groups 8 hours after the induction of Pancreatitis (P less than 0.05), but there was no statistical difference between any group. Likewise, there was no difference in gross or histologic changes in the pancreas of any group. The somatostatin analogue, octreotide, and the cholecystokinin receptor antagonist, L-364,718, did not ameliorate the effects of severe, bile-induced Pancreatitis in dogs.
Caroline S. Mansfield - One of the best experts on this subject based on the ideXlab platform.
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New directions in diagnosis and treatment of Canine acute Pancreatitis
2011Co-Authors: Caroline S. MansfieldAbstract:Acute Pancreatitis is an important disease in companion animal medicine, and diagnostic methodology available to veterinary practitioners is often limited. Evidence based principles for the management of this common disease are also lacking. This thesis explores the current diagnostics of Canine Pancreatitis and management of this condition, reviewing the literature across both the veterinary and human medical fields. Assessment of the specificity of Canine pancreatic-specific lipase (cPL) was made in a post-mortem study and calculated to be 82-92%, with a correlating sensitivity of 45-55%. A multi-centre study of dogs presenting with clinical signs consistent with acute Pancreatitis to assess a new laboratory test, serum Canine pancreatic elastase-1 (cPE-1) was also performed. This test had a sensitivity ranging from 66-79%, with a specificity of 92%. The sensitivity of both laboratory tests was greater in dogs with severe disease. To assess potential treatment options, a clinical severity score was established, with gut health, respiratory complications, cardiac complications, and blood pressure determining the final score. Retrospectively, plasma administration did not appear to be associated with treatment success, but this conclusion was limited by the retrospective nature of the study and small numbers of dogs. Out of the other factors, fasting for 3 or more days was the one most significantly associated with mortality. To begin assessment of nutritional modalities, pancreatic responses in healthy dogs to varying dietary fat composition (ranging from 4%DW to 16% DW) was assessed, with no statistical difference determined. On the basis of this, a pilot study of 10 dogs with severe Pancreatitis was undertaken, with 5 dogs fed enterally and another 5 dogs were given total parenteral nutrition (TPN). No differences in mortality or days of hospitalisation between the two were found, but there were significantly less episodes of vomiting or regurgitation in the dogs given food (p < 0.001). There were also more severe complications (4/5) in the TPN group compared to the enteral feeding group (2/5). In all, this thesis supports the new premise of enteral feeding of dogs with acute Pancreatitis early in the course of disease, determines the sensitivity and specificity of two diagnostic tests and has established an objective marker of disease severity.
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Assessing the severity of Canine Pancreatitis.
Research in veterinary science, 2003Co-Authors: Caroline S. Mansfield, Boyd R. Jones, T. SpillmanAbstract:The objective of this study was to determine whether laboratory testing currently available is able to provide prognostic information in Canine Pancreatitis. A prospective study of dogs with naturally occurring Pancreatitis was undertaken. Twenty-two cases with histologically confirmed pancreatic inflammation were included in the study. Each dog had routine haematology parameters, serum biochemistry (including lipase and amylase), serum trypsin-like immunoreactivity and trypsinogen activation peptides (TAP) in urine and plasma measured. Twelve of the dogs were classified as having severe disease. These dogs had statistically significant increases in urinary TAP–creatinine ratio (UTCR) measurement, serum lipase, serum phosphate and serum creatinine concentrations. Additionally dogs with severe Pancreatitis had significantly decreased urine specific gravity levels. The most sensitive and specific test to assess the severity of Pancreatitis was the measurement of UTCR.
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Plasma and urinary trypsinogen activation peptide in healthy dogs, dogs with Pancreatitis and dogs with other systemic diseases.
Australian veterinary journal, 2000Co-Authors: Caroline S. Mansfield, Boyd R. JonesAbstract:OBJECTIVE To determine the specificity and sensitivity of plasma and urinary trypsinogen activation peptide (TAP) concentrations in diagnosing Pancreatitis in dogs. DESIGN Retrospective analysis of clinical cases. PROCEDURE Dogs were classified into three groups: healthy animals, dogs with confirmed Pancreatitis and dogs with nonpancreatic disease, which clinically or biochemically resembled Pancreatitis. This last group was further subdivided into dogs with renal and those with nonrenal disease. The plasma and urinary TAP concentration was determined by a competitive enzyme immunoassay. Clinical cases additionally had serum trypsin-like immunoreactivity concentration measured, as well as radiography and ultrasound of the abdomen and further diagnostic procedures. Nonparametric analysis of variance (Kruskal-Wallis test) was performed using Statistix 4.0 program. RESULTS There was a wide range of urinary TAP concentration in healthy dogs (mean 52.30 nmol/L, standard deviation 55.25) that made interpretation of urinary TAP concentrations difficult in the other groups. There was a narrow reference range for plasma TAP (mean 2.67 nmol/L, standard deviation 0.93). Plasma and urinary TAP concentrations, as well as urinary TAP to creatinine ratio, were all increased in dogs that died with necrotising Pancreatitis. Values were not increased in mild, interstitial Pancreatitis. Increased plasma TAP concentrations were also present in dogs with severe renal disease. CONCLUSION Plasma TAP concentration is a good prognostic indicator in naturally occurring Pancreatitis in dogs. The failure of TAP to increase in mild Pancreatitis, and the increase present in severe renal disease, suggests its measurement has limited application as a sole diagnostic tool for Canine Pancreatitis. Further investigations are required in order to explain the large variability of urinary TAP concentration and the presence of circulating TAP in healthy dogs.
