The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Shing Hwa Liu - One of the best experts on this subject based on the ideXlab platform.
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Cantharidin Induced Oral Squamous Cell Carcinoma Cell Apoptosis via the JNK-Regulated Mitochondria and Endoplasmic Reticulum Stress-Related Signaling Pathways
2016Co-Authors: Kuan-i Lee, Mu-kuan Chen, Chun-ying Kuo, Chih-hsin Tang, Shing Hwa LiuAbstract:Oral cancer is a subtype of head and neck cancer which represents 2.65% of all human malignancies. Most of oral cancer is histopathologically diagnosed as oral squamous cell carcinoma (OSCC). OSCC is characterized by a high degree of local invasion and a high rate of metastasis to the cervical lymph nodes. How to prevention and treatment of OSCC is important and imperative. Here, we investigated the therapeutic effect and molecular mechanism of Cantharidin, an active compound isolated from blister beetles, on OSCC in vitro. Results showed that Cantharidin significantly decreased cell viability in human tongue squamous carcinoma-derived SAS, CAL-27, and SCC-4 cell lines. The further mechanistic studies were carried out in SAS cells. Cantharidin also significantly increased apoptosis-related signals, including caspase-9, caspase-7 and caspase-3 proteins. Besides, Cantharidin decreased mitochondrial transmembrane potential (MMP) and induced cytochrome c and apoptosis inducing factor (AIF) release. Cantharidin also increased Bax, Bid, and Bak protein expressions and decreased Bcl-2 protein expression. Cantharidin could also increase the endoplasmic reticulum (ER) stress signals, including the expressions of phosphorylated eIF-2α and CHOP, but not Grp78 and Grp94. Furthermore, Cantharidin reduced pro-caspase-12 protein expression. In signals of mitogen-activated protein kinases, Cantharidin increased the phosphorylation of JNK, but not ERK and p38. Transfection of shRNA-JNK to OSCC cells effectively reversed the Cantharidin-induced cell apoptotic signals, including the mitochondrial and ER stress-related signaling molecules. Taken together, these findings suggest that Cantharidin induces apoptosis in OSCC cells via the JNK-regulated mitochondria and ER stress-related signaling pathways.
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Cantharidin induces apoptosis through the calcium pkc regulated endoplasmic reticulum stress pathway in human bladder cancer cells
The American Journal of Chinese Medicine, 2015Co-Authors: Shing Hwa Liu, Kuan-i Lee, Koutong Huang, Kaimin Fang, Chengchieh Yen, Chihho Lai, Chunfa HuangAbstract:Bladder cancer is a common malignancy worldwide. However, there is still no effective therapy for bladder cancer. In this study, we investigated the cytotoxic effects of Cantharidin [a natural toxin produced (pure compound) from Chinese blister beetles (Mylabrisphalerata or Mylabriscichorii) and Spanish flies (Cantharis vesicatoria)] in human bladder cancer cell lines (including: T24 and RT4 cells). Treatment of human bladder cancer cells with Cantharidin significantly decreased cell viability. The increase in the expressions of caspase-3 activity and cleaved form of caspase-9/-7/-3 were also increased in Cantharidin-treated T24 cells. Furthermore, Cantharidin increased the levels of phospho-eIF2α and Grp78 and decreased the protein expression of procaspase-12, which was accompanied by the increase in calpain activity in T24 cells. Cantharidin was capable of increasing the intracellular Ca2+ and the phosphorylation of protein kinase C (PKC) in T24 cells. The addition of BAPTA/AM (a Ca2+ chelator) and RO320432 (a selective cell-permeable PKC inhibitor) effectively reversed the increase in caspase-3 and calpain activity, the phosphorylation levels of PKC and eIF2α and Grp78 protein expression, and the decrease in procaspase-12 expression induced by Cantharidin. Importantly, Cantharidin significantly decreased the tumor volume (a dramatic 71% reduction after 21 days of treatment) in nude mice xenografted with T24 cells. Taken together, these results indicate Cantharidin induced human bladder cancer cell apoptosis through a calcium/PKC-regulated ER stress pathway. These findings suggest that Cantharidin may be a novel and potential anticancer agent targeting on bladder cancer cells.
Yalin Zhang - One of the best experts on this subject based on the ideXlab platform.
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Chronic Sublethal Effects of Cantharidin on the Diamondback Moth Plutella xylostella (Lepidoptera: Plutellidae).
Toxins, 2015Co-Authors: Zhengyu Huang, Yalin ZhangAbstract:The diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), is a major pest of cruciferous vegetables worldwide. Cantharidin, a natural toxin isolated from blister beetles, has been reported to be toxic to P. xylostella. However, little is known on the chronic sublethal effects of Cantharidin on this species. In this study, we assessed the changes of susceptibility, development, reproduction and other demographic parameters in both the selected P. xylostella strain (Sub, selected by LC25 Cantharidin for consecutive 12 generations) and the revertant strain (SubR, derived from the Sub strain without being exposed to Cantharidin for 12 generations). Results revealed that the two strains maintained a relatively high-level susceptibility to Cantharidin. Severe adverse effects on the population dynamics and fitness in Sub strain were observed. In addition, repeated exposure of P. xylostella to sublethal concentration of Cantharidin resulted in negative effects on adult performance and deformities in adults. Although morphologically normal for individuals, the SubR strain exhibited a disadvantage in population growth rate. Our results showed that sublethal concentration of Cantharidin exhibited severe negative effects on population growth for longtime. These findings would be useful for assessing the potential effects and risk of Cantharidin on P. xylostella and for developing effective integrated pest management.
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The Toxicology and Biochemical Characterization of Cantharidin on Cydia pomonella.
Journal of economic entomology, 2015Co-Authors: Xue-qing Yang, Yalin ZhangAbstract:Cantharidin, a natural toxin produced by beetles in the families Meloidae and Oedemeridae, reported to be toxic to some pests, is being developed as a biopesticide in China. This study evaluates the toxicity and biochemical characterization of Cantharidin on the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), an economically important fruit pest, under both laboratory and field conditions. Laboratory dose response bioassays showed that the LC50 value of Cantharidin against neonate larvae was 0.057 mg ml(-1). Exposure of the larvae to 0.024 and 0.057 mg ml(-1) of Cantharidin resulted in significant reduction in larval body weight. Neonate larvae exposed to LC10 of Cantharidin showed increased glutathione S-transferase activity and significantly reduced the carboxylesterase and cytochrome P450-dependent mixed-function oxidase activities. Results also showed 16 and 25% ovicidal activity at concentrations of 0.057 and 0.14 mg ml(-1) of Cantharidin, respectively. Field trials demonstrated Cantharidin has a significant effect on both the first and second generations of C. pomonella larvae, but it exhibits a lower control efficiency than the chemical reference emamectin benzoate. Cantharidin may be considered a valuable tool for the control of codling moth.
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Lethal and sublethal effects of Cantharidin on the life history traits and population parameters of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae).
Pest management science, 2013Co-Authors: Rashid Ahmed Khan, M. Rashid, Dun Wang, Yalin ZhangAbstract:Background The cotton bollworm, Helicoverpa armigera (Hubner), is a serious and cosmopolitan pest of many economic crops. Its control has not been adequate owing to its resistance to many groups of insecticides. Toxicity of Cantharidin on armyworm and diamondback moth has already been reported. However, its toxicity on H. armigera has not been investigated previously. In this study, lethal and sublethal effects of Cantharidin on H. armigera under laboratory conditions are reported. Results Results showed gross abnormalities in the population parameters of H. armigera, ranging from larvae to adults. Reduction in larval weight and wing malformation were observed in the Cantharidin-treated population cohort, and higher mortality at the larval, pupal and adult stages was observed in Cantharidin-treated H. armigera compared with the control. Moreover, almost 5 times less fecundity was recorded in the treated population cohort. Fertility was also severely affected, and reduction in all population parameters was observed. Conclusion Cantharidin caused larval mortality and other serious abnormalities in H. armigera population parameters, and therefore may have positive implications for pest management decision-making process. More interestingly, the experiment revealed that Cantharidin in sublethal dose mimicked insect growth regulator insecticides. Furthermore, Cantharidin could be used as a precursor compound for the synthesis of new analogues and compounds to replace ineffective older compounds. © 2013 Society of Chemical Industry
Jonathan I Silverberg - One of the best experts on this subject based on the ideXlab platform.
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Efficacy and Safety of Topical Cantharidin Treatment for Molluscum Contagiosum and Warts: A Systematic Review
American Journal of Clinical Dermatology, 2018Co-Authors: Paras P. Vakharia, Nanette B Silverberg, Rishi Chopra, Jonathan I SilverbergAbstract:Background and Objective Topical Cantharidin is routinely used for the treatment of molluscum contagiosum and warts. The objective of this systematic review is to assess the efficacy and safety of topical Cantharidin treatment for molluscum contagiosum and warts. Methods We performed a systematic review of studies assessing topical Cantharidin treatment of molluscum contagiosum or warts. We searched the databases of Cochrane, EMBASE, GREAT, LILACS, MEDLINE, and Scopus. Two authors performed the study selection and data extraction. Results Twenty studies (1958–2018) met inclusion/exclusion criteria. Twelve studies assessed warts, and eight studies assessed molluscum contagiosum. Overall, 1752 patients were included (range 0.3–62 years; specified in 15 studies). Clearance rates with topical Cantharidin for molluscum contagiosum were variable (range 15.4–100%). Significant clearance of warts with maintenance of clearance was demonstrated with topical Cantharidin alone. Topical Cantharidin in combination with podophyllotoxin and salicylic acid demonstrated efficacy for plantar warts (pediatric and adult; clearance rate range 81–100%; four studies had 100% clearance), with the majority clearing after a single treatment. Satisfaction with Cantharidin therapy was high, especially in molluscum contagiosum. Pain (7–85.7%), blistering (10–100%), and hyper-/hypopigmentation (1.8–53.3%) were the most commonly occurring adverse effects with Cantharidin treatment. Conclusion Topical Cantharidin demonstrated clearance of warts, particularly in combination with podophyllotixin and salicylic acid, and modest benefit for pediatric molluscum contagiosum with good tolerability and safety.
Kuan-i Lee - One of the best experts on this subject based on the ideXlab platform.
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Cantharidin Induced Oral Squamous Cell Carcinoma Cell Apoptosis via the JNK-Regulated Mitochondria and Endoplasmic Reticulum Stress-Related Signaling Pathways
2016Co-Authors: Kuan-i Lee, Mu-kuan Chen, Chun-ying Kuo, Chih-hsin Tang, Shing Hwa LiuAbstract:Oral cancer is a subtype of head and neck cancer which represents 2.65% of all human malignancies. Most of oral cancer is histopathologically diagnosed as oral squamous cell carcinoma (OSCC). OSCC is characterized by a high degree of local invasion and a high rate of metastasis to the cervical lymph nodes. How to prevention and treatment of OSCC is important and imperative. Here, we investigated the therapeutic effect and molecular mechanism of Cantharidin, an active compound isolated from blister beetles, on OSCC in vitro. Results showed that Cantharidin significantly decreased cell viability in human tongue squamous carcinoma-derived SAS, CAL-27, and SCC-4 cell lines. The further mechanistic studies were carried out in SAS cells. Cantharidin also significantly increased apoptosis-related signals, including caspase-9, caspase-7 and caspase-3 proteins. Besides, Cantharidin decreased mitochondrial transmembrane potential (MMP) and induced cytochrome c and apoptosis inducing factor (AIF) release. Cantharidin also increased Bax, Bid, and Bak protein expressions and decreased Bcl-2 protein expression. Cantharidin could also increase the endoplasmic reticulum (ER) stress signals, including the expressions of phosphorylated eIF-2α and CHOP, but not Grp78 and Grp94. Furthermore, Cantharidin reduced pro-caspase-12 protein expression. In signals of mitogen-activated protein kinases, Cantharidin increased the phosphorylation of JNK, but not ERK and p38. Transfection of shRNA-JNK to OSCC cells effectively reversed the Cantharidin-induced cell apoptotic signals, including the mitochondrial and ER stress-related signaling molecules. Taken together, these findings suggest that Cantharidin induces apoptosis in OSCC cells via the JNK-regulated mitochondria and ER stress-related signaling pathways.
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Cantharidin induces apoptosis through the calcium pkc regulated endoplasmic reticulum stress pathway in human bladder cancer cells
The American Journal of Chinese Medicine, 2015Co-Authors: Shing Hwa Liu, Kuan-i Lee, Koutong Huang, Kaimin Fang, Chengchieh Yen, Chihho Lai, Chunfa HuangAbstract:Bladder cancer is a common malignancy worldwide. However, there is still no effective therapy for bladder cancer. In this study, we investigated the cytotoxic effects of Cantharidin [a natural toxin produced (pure compound) from Chinese blister beetles (Mylabrisphalerata or Mylabriscichorii) and Spanish flies (Cantharis vesicatoria)] in human bladder cancer cell lines (including: T24 and RT4 cells). Treatment of human bladder cancer cells with Cantharidin significantly decreased cell viability. The increase in the expressions of caspase-3 activity and cleaved form of caspase-9/-7/-3 were also increased in Cantharidin-treated T24 cells. Furthermore, Cantharidin increased the levels of phospho-eIF2α and Grp78 and decreased the protein expression of procaspase-12, which was accompanied by the increase in calpain activity in T24 cells. Cantharidin was capable of increasing the intracellular Ca2+ and the phosphorylation of protein kinase C (PKC) in T24 cells. The addition of BAPTA/AM (a Ca2+ chelator) and RO320432 (a selective cell-permeable PKC inhibitor) effectively reversed the increase in caspase-3 and calpain activity, the phosphorylation levels of PKC and eIF2α and Grp78 protein expression, and the decrease in procaspase-12 expression induced by Cantharidin. Importantly, Cantharidin significantly decreased the tumor volume (a dramatic 71% reduction after 21 days of treatment) in nude mice xenografted with T24 cells. Taken together, these results indicate Cantharidin induced human bladder cancer cell apoptosis through a calcium/PKC-regulated ER stress pathway. These findings suggest that Cantharidin may be a novel and potential anticancer agent targeting on bladder cancer cells.
Nanette B Silverberg - One of the best experts on this subject based on the ideXlab platform.
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Efficacy and Safety of Topical Cantharidin Treatment for Molluscum Contagiosum and Warts: A Systematic Review
American Journal of Clinical Dermatology, 2018Co-Authors: Paras P. Vakharia, Nanette B Silverberg, Rishi Chopra, Jonathan I SilverbergAbstract:Background and Objective Topical Cantharidin is routinely used for the treatment of molluscum contagiosum and warts. The objective of this systematic review is to assess the efficacy and safety of topical Cantharidin treatment for molluscum contagiosum and warts. Methods We performed a systematic review of studies assessing topical Cantharidin treatment of molluscum contagiosum or warts. We searched the databases of Cochrane, EMBASE, GREAT, LILACS, MEDLINE, and Scopus. Two authors performed the study selection and data extraction. Results Twenty studies (1958–2018) met inclusion/exclusion criteria. Twelve studies assessed warts, and eight studies assessed molluscum contagiosum. Overall, 1752 patients were included (range 0.3–62 years; specified in 15 studies). Clearance rates with topical Cantharidin for molluscum contagiosum were variable (range 15.4–100%). Significant clearance of warts with maintenance of clearance was demonstrated with topical Cantharidin alone. Topical Cantharidin in combination with podophyllotoxin and salicylic acid demonstrated efficacy for plantar warts (pediatric and adult; clearance rate range 81–100%; four studies had 100% clearance), with the majority clearing after a single treatment. Satisfaction with Cantharidin therapy was high, especially in molluscum contagiosum. Pain (7–85.7%), blistering (10–100%), and hyper-/hypopigmentation (1.8–53.3%) were the most commonly occurring adverse effects with Cantharidin treatment. Conclusion Topical Cantharidin demonstrated clearance of warts, particularly in combination with podophyllotixin and salicylic acid, and modest benefit for pediatric molluscum contagiosum with good tolerability and safety.
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childhood molluscum contagiosum experience with Cantharidin therapy in 300 patients
Journal of The American Academy of Dermatology, 2000Co-Authors: Nanette B Silverberg, Robert Sidbury, Anthony J ManciniAbstract:Abstract Background: Molluscum contagiosum (MC) is a common cutaneous infection in children. Cantharidin, a chemovesicant that is highly effective in treating MC, has lost favor with some physicians because of concerns over its safety. Objective: We attempted to determine the safety, efficacy, and parental satisfaction of Cantharidin therapy for MC in children who were treated in a pediatric dermatology clinic at a large referral hospital. Methods: A total of 537 charts of children who presented with MC were reviewed. We found 300 children who were treated with Cantharidin and who had parents available for telephone interview, which was performed in addition to chart review. Results: With Cantharidin therapy, 90% of patients experienced clearing and 8% improved. The average number of treatment visits was 2.1. Blisters occurred at sites of application in 92% of patients. Temporary burning, pain, erythema, or pruritus was reported in 6% to 37% of patients. No major side effects were reported, and no patients experienced secondary bacterial infection. A total of 95% of parents reported they would proceed with Cantharidin therapy again. Conclusion: To our knowledge ours is the largest retrospective series of childhood MC treated with Cantharidin. In these patients the therapy was extremely effective and well tolerated, and parental satisfaction was high. Cantharidin is a safe and effective therapy for MC in children. (J Am Acad Dermatol 2000;43:503–7.)