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Seyed Hadi Razavi - One of the best experts on this subject based on the ideXlab platform.
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optimisation and kinetic studies on the production of intracellular Canthaxanthin in fed batch cultures of dietzia natronolimnaea hs 1
Quality Assurance and Safety of Crops & Foods, 2015Co-Authors: Seyed Mohammad Taghi Gharibzahedi, Seyed Hadi Razavi, Mohammad MousaviAbstract:A critical optimisation and kinetic study on the production improvement of carotenoid pigment extracted from Dietzia natronolimnaea HS-1 under fed-batch fermentation was conducted. High pressure liquid chromatography revealed that the most abundant pigment biosynthesised by this bacterium was Canthaxanthin. The constant feeding rates (CFRs; 5.59-8.41 ml/h) for different concentrations of enzymatic hydrolysed molasses (EHM; 40.76-49.24 g/l) were optimised to achieve the highest cell biomass, total carotenoid and Canthaxanthin amounts using a response surface method. Spectrophotometric and optimisation analysis showed that the maximum cell mass (17.79 g/l), total carotenoid (16.37 mg/l) and Canthaxanthin (14.63 mg/l) can be obtained at 45.90 g/l EHM with a CFR of 6.40 ml/h. A very close agreement between experimental and predicted data was found. A substantial increase (123.4%) in production of Canthaxanthin was achieved after 152 h fed-batch process under optimal CFR mode as compared with batch process. Mo...
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enhancement of Canthaxanthin production from dietzia natronolimnaea hs 1 in a fed batch process using trace elements and statistical methods
Brazilian Journal of Chemical Engineering, 2010Co-Authors: M Nasri R Nasrabadi, Seyed Hadi RazaviAbstract:Under fed-batch process conditions, the statistical analysis of trace elements was performed by application of Plackett-Burman design (for screening tests) and response surface methodology (for predicting the optimal points) to achieve the highest level of Canthaxanthin production from Dietzia natronolimnaea HS-1. Plackett-Burman design was conducted on eleven trace elements (i. e., aluminum, boron, cobalt, copper, iron, magnesium, manganese, molybdenum, selenium, vanadium and zinc) to select out elements that significantly enhance the Canthaxanthin production of D. natronolimnaea HS-1. Plackett-Burman design revealed that Fe3+, Cu2+ and Zn2+ ions had the highest effect on Canthaxanthin production of D. natronolimnaea HS-1 (P<0.05). These three elements were used for further optimization. By means of response surface methodology for the fed-batch process, the optimum conditions to achieve the highest level of Canthaxanthin (8923±18 µg/L) were determined as follow: Fe3+ 30 ppm, Cu2+ 28.75 ppm and Zn2+ 27 ppm.
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use of response surface methodology in a fed batch process for optimization of tricarboxylic acid cycle intermediates to achieve high levels of Canthaxanthin from dietzia natronolimnaea hs 1
Journal of Bioscience and Bioengineering, 2010Co-Authors: Mohammad Reza Nasri Nasrabadi, Seyed Hadi RazaviAbstract:In this work, we applied statistical experimental design to a fed-batch process for optimization of tricarboxylic acid cycle (TCA) intermediates in order to achieve high-level production of Canthaxanthin from Dietzia natronolimnaea HS-1 cultured in beet molasses. A fractional factorial design (screening test) was first conducted on five TCA cycle intermediates. Out of the five TCA cycle intermediates investigated via screening tests, alfaketoglutarate, oxaloacetate and succinate were selected based on their statistically significant (P<0.05) and positive effects on Canthaxanthin production. These significant factors were optimized by means of response surface methodology (RSM) in order to achieve high-level production of Canthaxanthin. The experimental results of the RSM were fitted with a second-order polynomial equation by means of a multiple regression technique to identify the relationship between Canthaxanthin production and the three TCA cycle intermediates. By means of this statistical design under a fed-batch process, the optimum conditions required to achieve the highest level of Canthaxanthin (13172 + or - 25 microg l(-1)) were determined as follows: alfaketoglutarate, 9.69 mM; oxaloacetate, 8.68 mM; succinate, 8.51 mM.
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evaluation of coloring potential of dietzia natronolimnaea biomass as source of Canthaxanthin for egg yolk pigmentation
Asian-australasian Journal of Animal Sciences, 2009Co-Authors: M Esfahanimashhour, Hossein Moravej, Hassan Mehrabaniyeganeh, Seyed Hadi RazaviAbstract:: An experiment was conducted to evaluate the effect of different levels of extracted pigment from Dietzia natronolimnaea biomass as a source of Canthaxanthin in comparison with synthetic Canthaxanthin on egg yolk pigmentation. The experiment used a completely randomized design (CRD). A total of 63 laying hens, 68 weeks old, were used and the birds were allotted to 7 dietary treatments with each treatment replicated three times with three hens per replicate. Treatments consisted of 3 levels of synthetic Canthaxanthin (4, 8 and 16 ppm), 3 levels of extracted pigment from D. natronolimnaea biomass (4, 8 and 16 ppm) and control. Changes in yolk color were determined in 2 eggs taken at random, during the four week experimental period from each replicate. Supplementation of extracted pigment from D. natronolimnaea biomass had a significant effect on the color of egg yolks (p<0.05). Yolk color score of the control group was 6.83 in BASF color fan and the yolk color score of different extracted pigment levels was 11.00, 12.50 and 14.50, respectively. The yolk colors of different levels of synthetic Canthaxanthin were 12.00, 14.00 and 15.00, respectively. The effect of pigment supplementation on egg yolk color was better explained by polynomial response curves. The R 2 indicated that for 3 supplementation levels of each pigment studied, over 90% of the color variation could be explained by the pigment concentration. The egg yolk color after 15 and 30 days of storage was not significantly different, but boiling reduced egg yolk color significantly (p<0.05).
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optimization of Canthaxanthin production by dietzia natronolimnaea hs 1 from cheese whey using statistical experimental methods
Biochemical Engineering Journal, 2008Co-Authors: Faramarz Khodaiyan, Seyed Hadi Razavi, Seyed Mohammad MousaviAbstract:Abstract Sequential methodology combining a screening test by fractional factorial design and an optimization test by central composite design was applied to enhance Canthaxanthin production by Dietzia natronolimnaea HS-1 from cheese whey in shake flask cultures. Six variables (pH, luminous intensity, inoculum percent and concentrations of whey lactose, yeast extract and KH 2 PO 4 ) were studied with the screening test. The results revealed that three factors of pH, concentrations of whey lactose and yeast extract had greater influence on the Canthaxanthin production ( P
S J Davies - One of the best experts on this subject based on the ideXlab platform.
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tissue astaxanthin and Canthaxanthin distribution in rainbow trout oncorhynchus mykiss and atlantic salmon salmo salar
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2006Co-Authors: G I Page, S J DaviesAbstract:A comparative investigation of tissue carotenoid distribution between rainbow trout, Oncorhynchus mykiss, and Atlantic salmon, Salmo salar, was undertaken to identify the relative efficiency of utilization of astaxanthin and Canthaxanthin. Higher apparent digestibility coefficients (ADCs) (96% in trout vs. 28-31% in salmon; P<0.05), and pigment retention efficiencies (11.5-12.5% in trout vs. 5.5% in salmon; P<0.05), for both astaxanthin and Canthaxanthin, were observed for rainbow trout. Astaxanthin deposition was higher than Canthaxanthin in rainbow trout, while the reverse was true for Atlantic salmon, suggesting species-specificity in carotenoid utilization. The white muscle (95% in trout vs. 93% in salmon) and kidneys (0.5% in trout vs. 0.2% in salmon) represented higher proportions of the total body carotenoid pool in rainbow trout than in Atlantic salmon (P<0.05), whereas the liver was a more important storage organ in Atlantic salmon (2-6% in salmon vs. 0.2% in trout; P<0.05). The liver and kidney appeared to be important sites of carotenoid catabolism based on the relative proportion of the peak chromatogram of the fed carotenoid in both species, with the pyloric caecae and hind gut being more important in Atlantic salmon than in the rainbow trout. Liver catabolism is suspected to be a critical determinant in carotenoid clearance, with higher catabolism expected in Atlantic salmon than in rainbow trout.
Debjani Dutta - One of the best experts on this subject based on the ideXlab platform.
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a statistically motivated choice of process parameters for the improvement of Canthaxanthin production by dietzia maris nit d accession number hm151403
Revista Mexicana De Ingenieria Quimica, 2014Co-Authors: J De Neve, Gargi Goswami, Surabhi Chaudhuri, Abhishek Dutta, Debjani DuttaAbstract:Dietzia maris NIT-D, a producer of Canthaxanthin, is isolated during routine screening of pigment producing bacteria. The effects of process parameters, namely temperature, pH, shaker speed, percentage inoculum, medium volume, and concentration of glucose on the Canthaxanthin production are studied by using response surface design methodology. The optimal conditions are temperature = 30 degrees C, pH = 5.9, shaker speed = 125 rpm, inoculum = 1.9 %, volume = 50 mL, and glucose = 15 g L-1, resulting in a Canthaxanthin production of 152 mg L-1, which is 25% higher than that of a recently reported study.
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optimization of process parameters by response surface methodology and kinetic modeling for batch production of Canthaxanthin by dietzia maris nit d accession number hm151403
Bioprocess and Biosystems Engineering, 2012Co-Authors: Gargi Goswami, Samarprit Chakraborty, Surabhi Chaudhuri, Debjani DuttaAbstract:Dietzia maris NIT-D, a Canthaxanthin producer, was isolated during routine screening of pigment-producing bacteria. Response surface methodology was applied for statistical designing of process parameters for biomass and Canthaxanthin production. The effects of four process parameters (considered as independent variables), namely temperature (10–30 °C), pH (4.75–5.75), shaker speed (75–135 rpm) and percentage inoculum (0.5–2.5 %) on the biomass and Canthaxanthin yield (considered as dependent variables) were studied. As much as 122 mg L−1 of Canthaxanthin was obtained when Dietzia maris NIT-D was incubated for 120 h at 25 °C and 120 rpm, initial pH and percentage inoculum being 5.5 and 2 % respectively. The pigment yield is the highest reported till date, with Dietzia maris as the test organism. The maximum biomass yield was 7.39 g L−1 under optimized process parameters. The predicted values were also verified by validation experiments in 5-day fermentation. Different mathematical models were used to describe growth and production, considering the effect of glucose in batch mode. The kinetic constants were calculated by fitting the experimental data to the models. Cell growth was inhibited beyond a glucose concentration of 15 g L−1. Andrews’ model gave the best fit with a R2 value of 0.9993. During the exponential growth phase, the specific growth rate was found to remain fairly constant with respect to time. There was no inhibitory effect due to intracellular product accumulation for all concentrations of glucose. This observation is the first of its kind, as previous studies have reported that increasing accumulation of intracellular carotenoid exerts greater degree of inhibition on growth.
G I Page - One of the best experts on this subject based on the ideXlab platform.
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tissue astaxanthin and Canthaxanthin distribution in rainbow trout oncorhynchus mykiss and atlantic salmon salmo salar
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2006Co-Authors: G I Page, S J DaviesAbstract:A comparative investigation of tissue carotenoid distribution between rainbow trout, Oncorhynchus mykiss, and Atlantic salmon, Salmo salar, was undertaken to identify the relative efficiency of utilization of astaxanthin and Canthaxanthin. Higher apparent digestibility coefficients (ADCs) (96% in trout vs. 28-31% in salmon; P<0.05), and pigment retention efficiencies (11.5-12.5% in trout vs. 5.5% in salmon; P<0.05), for both astaxanthin and Canthaxanthin, were observed for rainbow trout. Astaxanthin deposition was higher than Canthaxanthin in rainbow trout, while the reverse was true for Atlantic salmon, suggesting species-specificity in carotenoid utilization. The white muscle (95% in trout vs. 93% in salmon) and kidneys (0.5% in trout vs. 0.2% in salmon) represented higher proportions of the total body carotenoid pool in rainbow trout than in Atlantic salmon (P<0.05), whereas the liver was a more important storage organ in Atlantic salmon (2-6% in salmon vs. 0.2% in trout; P<0.05). The liver and kidney appeared to be important sites of carotenoid catabolism based on the relative proportion of the peak chromatogram of the fed carotenoid in both species, with the pyloric caecae and hind gut being more important in Atlantic salmon than in the rainbow trout. Liver catabolism is suspected to be a critical determinant in carotenoid clearance, with higher catabolism expected in Atlantic salmon than in rainbow trout.
Georges Choubert - One of the best experts on this subject based on the ideXlab platform.
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muscle carotenoid content and colour of farmed rainbow trout fed astaxanthin or Canthaxanthin as affected by cooking and smoke curing procedures
International Journal of Food Science and Technology, 2007Co-Authors: Georges Choubert, J M Blanc, Corinne CourvalinAbstract:Summary. Carotenoids and colour characteristics were studied in raw, steamed and smoke-cured muscle of rainbow trout fed synthetic astaxanthin (50 or 100mgkg-1 diet) or Canthaxanthin (100mgkg-1 diet) for 15 months. In raw muscle main carotenoid concentration was strongly correlated with hue, chroma, and lightness. Diet and sex effects were significant on most traits, with little or no interaction. Female muscles contained much more carotenoid and were more coloured than male ones. Astaxanthin fed fish were more red than Canthaxanthin fed fish. Significant mean effects of cooking were an apparent increase of carotenoid concentration and an increase of lightness. Hue and chroma were practically unchanged with high correlations between raw and cooked muscle measurements. Lightness increased and differences between sexes and individuals were reduced. Smoke-curing induced an increase of carotenoid concentration and a decrease of hue and lightness. Hue of Canthaxanthin fed fish when smoke-cured was similar to that of raw fish fed astaxanthin. Lightness, still dependant on that of the raw muscle, was reduced and differences among diets, sexes and individuals were reduced.
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carotenoid pigments of the green alga haematococcus pluvialis assay on rainbow trout oncorhynchus mykiss pigmentation in comparison with synthetic astaxanthin and Canthaxanthin
Aquaculture, 1993Co-Authors: Georges Choubert, Olivier HeinrichAbstract:Abstract The green alga Haematococcus pluvialis was found to contain β -carotene (2.2%), astaxanthin (free A feeding trial was conducted to study the effects of dietary algal incorporation on muscle pigmentation of rainbow trout in comparison with synthetic astaxanthin and Canthaxanthin alone or mixed (astaxanthin 48% and Canthaxanthin 52%). After 4 weeks of feeding, physical colour measurements showed that increased pigmentation of the trout muscle caused an increase in chroma and a reduction in hue and lightness. Muscle of trout fed algae contained 6.2 mg carotenoid/kg versus 12.7 mg/kg for trout fed the mixture of the two synthetic carotenoids, and 11.8 and 10.1 mg/kg for trout fed synthetic astaxanthin and Canthaxanthin, respectively. Carotenoid retention in the muscle of trout fed algae was 1.5% which was less than the retention of the carotenoid mixture (3.1%).