The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Orlova V.s. - One of the best experts on this subject based on the ideXlab platform.
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Antitumor enzyme L-lyzine-α-oxidase from Trichoderma harzianum Rifai F-180 and investigation of its action on L-lysine oxidation by Capillar Electrophoresis
2020Co-Authors: Smirnova I.p., Shkinev V.m., Rudnev A.v., Kuznetsova O.m., Karimova E.v., Orlova V.s.Abstract:Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-a-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-α-oxidase on oxidation of L-lysine was investigated for the first time by Capillary Electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7×103 RU/sec) was determined. The use of the antitumor enzyme L-lysine-a-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess
V S Orlova - One of the best experts on this subject based on the ideXlab platform.
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antitumor enzyme l lyzine alpha oxidase from trichoderma harzianum rifai f 180 and investigation of its action on l lysine oxidation by Capillar Electrophoresis
Antibiotics and chemoterapy, 2014Co-Authors: I P Smirnova, V M Shkinev, A V Rudnev, O M Kuznetsova, E V Karimova, V S OrlovaAbstract:Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-alpha-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-alpha-oxidase on oxidation of L-lysine was investigated for the first time by Capillary Electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7 x 10(-3) RU/sec) was determined. The use of the antitumor enzyme L-lysine-alpha-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess.
Stuart A Oehrle - One of the best experts on this subject based on the ideXlab platform.
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analysis of nitramine and nitroaromatic explosives in pink water by Capillar Electrophoresis
Electrophoresis, 1997Co-Authors: Stuart A OehrleAbstract:Analysis of nitramine and nitroaromatic explosives is of interest for environmental applications. Analysis of 14 nitramine and nitroaromatic explosives was accomplished by micellar electrokinetic Capillary chromatography (MECC). Analysis times under 14 min were possible employing a phosphate-sodium dodecyl sulfate (SDS) electrolyte and direct UV detection at 254 nm. Analysis of various waste water samples (called “pink water”) from ammunition plants was performed.
Smirnova I.p. - One of the best experts on this subject based on the ideXlab platform.
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Antitumor enzyme L-lyzine-α-oxidase from Trichoderma harzianum Rifai F-180 and investigation of its action on L-lysine oxidation by Capillar Electrophoresis
2020Co-Authors: Smirnova I.p., Shkinev V.m., Rudnev A.v., Kuznetsova O.m., Karimova E.v., Orlova V.s.Abstract:Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-a-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-α-oxidase on oxidation of L-lysine was investigated for the first time by Capillary Electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7×103 RU/sec) was determined. The use of the antitumor enzyme L-lysine-a-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess
Andrzej Kaźmierczak - One of the best experts on this subject based on the ideXlab platform.
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Cytochemical and immunocytochemical studies of the localization of histones and protamine-type proteins in spermatids of Chara vulgaris and Chara tomentosa.
Folia histochemica et cytobiologica, 2007Co-Authors: Katarzyna Popłońska, Agnieszka Wojtczak, Maria Kwiatkowska, Andrzej KaźmierczakAbstract:Spermiogenesis in Chara algae, which has been divided into 10 phases (sp I-X), is similar to spermiogenesis in animals. The most important process during spermiogenesis in animals is remodeling of chromatin leading to "sleeping genome", being the result the exchange of histone proteins into protamine-like proteins. Cytochemical studies showed in both Chara species (C. vulgaris, C. tomentosa) that at spI-IV phases only histones were present, at spV-VIII phases--the amount of nuclear protamine-type proteins progressively increased and that of histones decreased while at spIX-X only pro-tamine-type proteins were present. This was also confirmed with Capillar Electrophoresis. In order to localize more precisely both histones and protamines the immunocytochemical studies with the use of anti-protamine antibodies (protamine-type proteins were obtained from C. tomentosa antheridia) and anti-histone H3 antibodies, have been carried out. More specific immunocytochemical studies confirmed cytochemical results including the exchange of histones into protamine-type during spermiogenesis (spV-VIII) in both Chara species. At phase V spermiogenesis these strong strand-like anti-protamine signals were observed in cytoplasm which might suggest that protamine synthesis took place in ER.
