The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform

Hans-joachim Knölker - One of the best experts on this subject based on the ideXlab platform.

Olga Kataeva - One of the best experts on this subject based on the ideXlab platform.

Arndt W. Schmidt - One of the best experts on this subject based on the ideXlab platform.

Christian Brütting - One of the best experts on this subject based on the ideXlab platform.

Samir Bhattacharya - One of the best experts on this subject based on the ideXlab platform.

  • a Carbazole Alkaloid deactivates mtor through the suppression of rictor and that induces apoptosis in lung cancer cells
    Molecular and Cellular Biochemistry, 2015
    Co-Authors: Priyajit Chatterjee, Soma Seal, Sandip Mukherjee, Rakesh Kundu, Mantu Bhuyan, Nabin C Barua, Pranab Kumar Baruah, Santi Sinha P Babu, Samir Bhattacharya
    Abstract:

    Non-small cell lung cancer (NSCLC) is known to be a difficult cancer to treat because of its poor prognosis, limited option for surgery, and resistance to chemo or radiotherapy. In this study, we have demonstrated that suppression of rictor expression in A549 and H1299 NSCLC cells by mahanine, a Carbazole Alkaloid, disrupted constitutive activation of mTOR and Akt. Mahanine suppression of rictor gene expression and consequent attenuation of its protein expression affected the inhibition of mTOR (Ser-2481) and Akt (Ser-473) phosphorylation. Since mahanine treatment revealed this new insight of rictor-mTOR relationship, we examined an association between mTOR activation with rictor expression. Interestingly, in rictor knockdown (KD) NSCLC cells, mTOR activation was significantly impaired. Transfection of rictor over-expression vector into the NSCLC cells reversed this situation. In fact, both rictor KD and mahanine treated cells showed considerably depleted phospho-mTOR level. These results indicate that rictor is required to maintain constitutive activation of mTOR in lung cancer cells. When mTOR kinase activity in rictor KD cells was examined with Akt as substrate, a significant reduction of Akt phosphorylation indicated impairment of mTOR kinase potentiality. Disruption of mTOR and Akt activation caused drastic mortality of NSCLC cancer cells through apoptosis. Hence, our study reveals a new dimension in mTOR-rictor relationship, where rictor stands to be a suitable therapeutic target for lung cancer.

  • mahanine restores rassf1a expression by down regulating dnmt1 and dnmt3b in prostate cancer cells
    Molecular Cancer, 2013
    Co-Authors: Soumik Agarwal, Nabin C Barua, Samir Bhattacharya, Karishma S Amin, Shankar Jagadeesh, Gokul Baishay, P G Rao, Partha P Banerjee
    Abstract:

    Background Hypermethylation of the promoter of the tumor suppressor gene RASSF1A silences its expression and has been found to be associated with advanced grade prostatic tumors. The DNA methyltransferase (DNMT) family of enzymes are known to be involved in the epigenetic silencing of gene expression, including RASSF1A, and are often overexpressed in prostate cancer. The present study demonstrates how mahanine, a plant-derived Carbazole Alkaloid, restores RASSF1A expression by down-regulating specific members of the DNMT family of proteins in prostate cancer cells.