The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Kwok-yin Wong - One of the best experts on this subject based on the ideXlab platform.
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Trityl-derivatized Carbohydrates immobilized on a polystyrene microplate.
Carbohydrate research, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Abstract Carbohydrate biosensors, including carbohydrate arrays, are attracting increased attention for the comprehensive and high-throughput investigation of protein–carbohydrate interactions. Here, we describe an effective approach to fabricating a robust microplate-based carbohydrate array capable of probing protein binding and screening for inhibitors in a high-throughout manner. This approach involves the derivatization of Carbohydrates with a trityl group through an alkyl linker and the immobilization of the trityl-derivatized Carbohydrates (mannose and maltose) onto microplates noncovalently to construct carbohydrate arrays. The trityl carbohydrate derivative has very good immobilization efficiency for polystyrene microplates and strong resistance to aqueous washing. The carbohydrate arrays can probe the interactions with the lectin Concanavalin A and screen this protein for the well-known inhibitors methyl α- d -mannopyranoside and methyl α- d -glucopyranoside in a high-throughput manner. The method described in this paper represents a convenient way of fabricating robust noncovalent carbohydrate arrays on microplates and offers a convenient platform for high-throughput drug screening.
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Covalent immobilization of Carbohydrates on sol–gel-coated microplates
Analyst, 2008Co-Authors: Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Lian-quan Gu, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
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Covalent immobilization of Carbohydrates on sol-gel-coated microplates.
The Analyst, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
Hei-leung Pang - One of the best experts on this subject based on the ideXlab platform.
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Trityl-derivatized Carbohydrates immobilized on a polystyrene microplate.
Carbohydrate research, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Abstract Carbohydrate biosensors, including carbohydrate arrays, are attracting increased attention for the comprehensive and high-throughput investigation of protein–carbohydrate interactions. Here, we describe an effective approach to fabricating a robust microplate-based carbohydrate array capable of probing protein binding and screening for inhibitors in a high-throughout manner. This approach involves the derivatization of Carbohydrates with a trityl group through an alkyl linker and the immobilization of the trityl-derivatized Carbohydrates (mannose and maltose) onto microplates noncovalently to construct carbohydrate arrays. The trityl carbohydrate derivative has very good immobilization efficiency for polystyrene microplates and strong resistance to aqueous washing. The carbohydrate arrays can probe the interactions with the lectin Concanavalin A and screen this protein for the well-known inhibitors methyl α- d -mannopyranoside and methyl α- d -glucopyranoside in a high-throughput manner. The method described in this paper represents a convenient way of fabricating robust noncovalent carbohydrate arrays on microplates and offers a convenient platform for high-throughput drug screening.
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Covalent immobilization of Carbohydrates on sol–gel-coated microplates
Analyst, 2008Co-Authors: Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Lian-quan Gu, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
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Covalent immobilization of Carbohydrates on sol-gel-coated microplates.
The Analyst, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
Hans Schnyder - One of the best experts on this subject based on the ideXlab platform.
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Pre-anthesis reserve utilization for protein and carbohydrate synthesis in grains of wheat
Plant Physiology, 1999Co-Authors: Thomas Gebbing, Hans SchnyderAbstract:We assessed the contribution of pre-anthesis reserve C to protein and carbohydrate deposition in grains of wheat (Triticum aestivum L.) using a new approach comprised of steady-state 13C/12C labeling and separation of the protein and carbohydrate fractions of mature grains. Experiments were performed with two spring wheat cultivars (Kadett and Star) grown with differential N fertilizer supply over 2 years. Pre-anthesis reserves contributed between 30% and 47% of the C in protein and 8% to 27% of the C in Carbohydrates of grains. Partitioning of pre-anthesis C among the grain fractions was strongly dependent on the C/N (w/w) ratio in mobilized pre-anthesis biomass (r2 = 0.92). There appeared to be no significant exchange of pre-anthesis C between amino acids and Carbohydrates during redistribution. The mean apparent efficiency of mobilized carbohydrate-C use in grain filling (MECHO, estimated as the mass of pre-anthesis C deposited in grain Carbohydrates per gram of pre-anthesis C mobilized from Carbohydrates in vegetative plant parts) was 0.72, whereas that of protein-C (MEP) was 0.56. However, MEP and MECHO varied among treatments. MECHO increased with increasing contributions of water-soluble Carbohydrates to total pre-anthesis carbohydrate mobilization. MEP decreased with increasing residence time of protein in vegetative biomass. Possible causes for variability of MEP and MECHO are discussed.
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Pre-Anthesis Reserve Utili izat'on for Proten and Carbohydrate Synthesis 'n Grains of VVheat'
1999Co-Authors: Thomas Gebbing, Hans SchnyderAbstract:We assessed the contribution of pre-anthesis reserve C to protein and carbohydrate deposition in grains of wheat (Triticum aestivum L.) using a new approach comprised of steady-state 13C/12C labeling and separation of the protein and carbohydrate fractions of mature grains. Experiments were performed with two spring wheat cultivars (Kadett and Star) grown with differential N fertilizer supply over 2 years. Pre-anthesis reserves contributed between 30% and 47% of the C in protein and 8% to 27% of the C in Carbohydrates of grains. Partitioning of pre-anthesis C among the grain fractions was strongly dependent on the C/N (w/w) ratio in mobilized preanthesis biomass (r2 = 0.92). There appeared to be no significant exchange of pre-anthesis C between amino acids and Carbohydrates during redistribution. The mean apparent efficiency of mobilized carbohydrate-C use in grain filling (MECHO, estimated as the mass of pre-anthesis C deposited in grain Carbohydrates per gram of preanthesis C mobilized from Carbohydrates in vegetative plant parts) was 0.72, whereas that of protein-C (MEp) was 0.56. However, MEp and MECHO varied among treatments. MECHo increased with increasing contributions of water-soluble Carbohydrates to total preanthesis carbohydrate mobilization. ME, decreased with increasing residence time of protein in vegetative biomass. Possible causes for variability of MEp and MECHO are discussed.
Zhi-shu Huang - One of the best experts on this subject based on the ideXlab platform.
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Trityl-derivatized Carbohydrates immobilized on a polystyrene microplate.
Carbohydrate research, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Abstract Carbohydrate biosensors, including carbohydrate arrays, are attracting increased attention for the comprehensive and high-throughput investigation of protein–carbohydrate interactions. Here, we describe an effective approach to fabricating a robust microplate-based carbohydrate array capable of probing protein binding and screening for inhibitors in a high-throughout manner. This approach involves the derivatization of Carbohydrates with a trityl group through an alkyl linker and the immobilization of the trityl-derivatized Carbohydrates (mannose and maltose) onto microplates noncovalently to construct carbohydrate arrays. The trityl carbohydrate derivative has very good immobilization efficiency for polystyrene microplates and strong resistance to aqueous washing. The carbohydrate arrays can probe the interactions with the lectin Concanavalin A and screen this protein for the well-known inhibitors methyl α- d -mannopyranoside and methyl α- d -glucopyranoside in a high-throughput manner. The method described in this paper represents a convenient way of fabricating robust noncovalent carbohydrate arrays on microplates and offers a convenient platform for high-throughput drug screening.
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Covalent immobilization of Carbohydrates on sol–gel-coated microplates
Analyst, 2008Co-Authors: Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Lian-quan Gu, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
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Covalent immobilization of Carbohydrates on sol-gel-coated microplates.
The Analyst, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
Pak-ho Chan - One of the best experts on this subject based on the ideXlab platform.
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Trityl-derivatized Carbohydrates immobilized on a polystyrene microplate.
Carbohydrate research, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Abstract Carbohydrate biosensors, including carbohydrate arrays, are attracting increased attention for the comprehensive and high-throughput investigation of protein–carbohydrate interactions. Here, we describe an effective approach to fabricating a robust microplate-based carbohydrate array capable of probing protein binding and screening for inhibitors in a high-throughout manner. This approach involves the derivatization of Carbohydrates with a trityl group through an alkyl linker and the immobilization of the trityl-derivatized Carbohydrates (mannose and maltose) onto microplates noncovalently to construct carbohydrate arrays. The trityl carbohydrate derivative has very good immobilization efficiency for polystyrene microplates and strong resistance to aqueous washing. The carbohydrate arrays can probe the interactions with the lectin Concanavalin A and screen this protein for the well-known inhibitors methyl α- d -mannopyranoside and methyl α- d -glucopyranoside in a high-throughput manner. The method described in this paper represents a convenient way of fabricating robust noncovalent carbohydrate arrays on microplates and offers a convenient platform for high-throughput drug screening.
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Covalent immobilization of Carbohydrates on sol–gel-coated microplates
Analyst, 2008Co-Authors: Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Lian-quan Gu, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.
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Covalent immobilization of Carbohydrates on sol-gel-coated microplates.
The Analyst, 2008Co-Authors: Lan Zou, Hei-leung Pang, Pak-ho Chan, Zhi-shu Huang, Kwok-yin WongAbstract:Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein–carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact Carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol–gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of Carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to Carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact Carbohydrates on sol–gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein–carbohydrate interactions in a high-throughput manner.