The Experts below are selected from a list of 342 Experts worldwide ranked by ideXlab platform

Charles Wang - One of the best experts on this subject based on the ideXlab platform.

  • Single-cell transcriptome and epigenomic reprogramming of cardiomyocyte-derived Cardiac progenitor cells.
    Scientific Data, 2016
    Co-Authors: Xin Chen, Tushar Chakravarty, Yiqiang Zhang, Xiaojin Li, Jiang F. Zhong, Charles Wang
    Abstract:

    The molecular basis underlying the dedifferentiation of mammalian adult cardiomyocytes (ACMs) into myocyte-derived Cardiac progenitor cells (mCPCs) during Cardiac Tissue Regeneration is poorly understood. We present data integrating single-cell transcriptome and whole-genome DNA methylome analyses of mouse mCPCs to understand the epigenomic reprogramming governing their intrinsic cellular plasticity. Compared to parental cardiomyocytes, mCPCs display epigenomic reprogramming with many differentially-methylated regions, both hypermethylated and hypomethylated, across the entire genome. Correlating well with the methylome, our single-cell transcriptomic data show that the genes encoding Cardiac structure and function proteins are remarkably down-regulated in mCPCs, while those for cell cycle, proliferation, and stemness are significantly up-regulated. In addition, implanting mCPCs into infarcted mouse myocardium improves Cardiac function with augmented left ventricular ejection fraction. This dataset suggests that the cellular plasticity of mammalian cardiomyocytes is the result of a well-orchestrated epigenomic reprogramming and a subsequent global transcriptomic alteration. Understanding cardiomyocyte epigenomic reprogramming may enable the design of future clinical therapies that induce Cardiac Regeneration, and prevent heart failure.

  • Single-cell transcriptome and epigenomic reprogramming of cardiomyocyte-derived Cardiac progenitor cells
    Scientific Data, 2016
    Co-Authors: Xin Chen, Tushar Chakravarty, Yiqiang Zhang, Xiaojin Li, Jiang F. Zhong, Charles Wang
    Abstract:

    The molecular basis underlying the dedifferentiation of mammalian adult cardiomyocytes (ACMs) into myocyte-derived Cardiac progenitor cells (mCPCs) during Cardiac Tissue Regeneration is poorly understood. We present data integrating single-cell transcriptome and whole-genome DNA methylome analyses of mouse mCPCs to understand the epigenomic reprogramming governing their intrinsic cellular plasticity. Compared to parental cardiomyocytes, mCPCs display epigenomic reprogramming with many differentially-methylated regions, both hypermethylated and hypomethylated, across the entire genome. Correlating well with the methylome, our single-cell transcriptomic data show that the genes encoding Cardiac structure and function proteins are remarkably down-regulated in mCPCs, while those for cell cycle, proliferation, and stemness are significantly up-regulated. In addition, implanting mCPCs into infarcted mouse myocardium improves Cardiac function with augmented left ventricular ejection fraction. This dataset suggests that the cellular plasticity of mammalian cardiomyocytes is the result of a well-orchestrated epigenomic reprogramming and a subsequent global transcriptomic alteration. Understanding cardiomyocyte epigenomic reprogramming may enable the design of future clinical therapies that induce Cardiac Regeneration, and prevent heart failure. Design Type(s) parallel group design • cell type comparison design Measurement Type(s) transcription profiling assay • DNA residue methylation Technology Type(s) real time reverse-transcription polymerase chain reaction assay • DNA microarray • DNA methylation profiling by array assay Factor Type(s) Cell • microarray platform Sample Characteristic(s) Mus musculus • Cardiac muscle Tissue Machine-accessible metadata file describing the reported data (ISA-Tab format)

Marcelle Machluf - One of the best experts on this subject based on the ideXlab platform.

  • electrospun extracellular matrix paving the way to tailor made natural scaffolds for Cardiac Tissue Regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native Tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the Tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate bioactive material for the Regeneration of different organs. Particularly for Cardiac Regeneration, ECM is studied as a patch and injectable scaffolds, which improve Cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine Cardiac ECM using electrospinning technology, is developed. This unique electrospun Cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of Cardiac ECM ,and ,above all, preserves key Cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural materials as tailor-made, well-defined structures.

  • Electrospun Extracellular Matrix: Paving the Way to Tailor‐Made Natural Scaffolds for Cardiac Tissue Regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native Tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the Tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate bioactive material for the Regeneration of different organs. Particularly for Cardiac Regeneration, ECM is studied as a patch and injectable scaffolds, which improve Cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine Cardiac ECM using electrospinning technology, is developed. This unique electrospun Cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of Cardiac ECM ,and ,above all, preserves key Cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural materials as tailor-made, well-defined structures.

Xin Chen - One of the best experts on this subject based on the ideXlab platform.

  • Single-cell transcriptome and epigenomic reprogramming of cardiomyocyte-derived Cardiac progenitor cells.
    Scientific Data, 2016
    Co-Authors: Xin Chen, Tushar Chakravarty, Yiqiang Zhang, Xiaojin Li, Jiang F. Zhong, Charles Wang
    Abstract:

    The molecular basis underlying the dedifferentiation of mammalian adult cardiomyocytes (ACMs) into myocyte-derived Cardiac progenitor cells (mCPCs) during Cardiac Tissue Regeneration is poorly understood. We present data integrating single-cell transcriptome and whole-genome DNA methylome analyses of mouse mCPCs to understand the epigenomic reprogramming governing their intrinsic cellular plasticity. Compared to parental cardiomyocytes, mCPCs display epigenomic reprogramming with many differentially-methylated regions, both hypermethylated and hypomethylated, across the entire genome. Correlating well with the methylome, our single-cell transcriptomic data show that the genes encoding Cardiac structure and function proteins are remarkably down-regulated in mCPCs, while those for cell cycle, proliferation, and stemness are significantly up-regulated. In addition, implanting mCPCs into infarcted mouse myocardium improves Cardiac function with augmented left ventricular ejection fraction. This dataset suggests that the cellular plasticity of mammalian cardiomyocytes is the result of a well-orchestrated epigenomic reprogramming and a subsequent global transcriptomic alteration. Understanding cardiomyocyte epigenomic reprogramming may enable the design of future clinical therapies that induce Cardiac Regeneration, and prevent heart failure.

  • Single-cell transcriptome and epigenomic reprogramming of cardiomyocyte-derived Cardiac progenitor cells
    Scientific Data, 2016
    Co-Authors: Xin Chen, Tushar Chakravarty, Yiqiang Zhang, Xiaojin Li, Jiang F. Zhong, Charles Wang
    Abstract:

    The molecular basis underlying the dedifferentiation of mammalian adult cardiomyocytes (ACMs) into myocyte-derived Cardiac progenitor cells (mCPCs) during Cardiac Tissue Regeneration is poorly understood. We present data integrating single-cell transcriptome and whole-genome DNA methylome analyses of mouse mCPCs to understand the epigenomic reprogramming governing their intrinsic cellular plasticity. Compared to parental cardiomyocytes, mCPCs display epigenomic reprogramming with many differentially-methylated regions, both hypermethylated and hypomethylated, across the entire genome. Correlating well with the methylome, our single-cell transcriptomic data show that the genes encoding Cardiac structure and function proteins are remarkably down-regulated in mCPCs, while those for cell cycle, proliferation, and stemness are significantly up-regulated. In addition, implanting mCPCs into infarcted mouse myocardium improves Cardiac function with augmented left ventricular ejection fraction. This dataset suggests that the cellular plasticity of mammalian cardiomyocytes is the result of a well-orchestrated epigenomic reprogramming and a subsequent global transcriptomic alteration. Understanding cardiomyocyte epigenomic reprogramming may enable the design of future clinical therapies that induce Cardiac Regeneration, and prevent heart failure. Design Type(s) parallel group design • cell type comparison design Measurement Type(s) transcription profiling assay • DNA residue methylation Technology Type(s) real time reverse-transcription polymerase chain reaction assay • DNA microarray • DNA methylation profiling by array assay Factor Type(s) Cell • microarray platform Sample Characteristic(s) Mus musculus • Cardiac muscle Tissue Machine-accessible metadata file describing the reported data (ISA-Tab format)

Armand Keating - One of the best experts on this subject based on the ideXlab platform.

  • Collagen scaffold enhances the regenerative properties of mesenchymal stromal cells.
    PLOS ONE, 2017
    Co-Authors: Iran Rashedi, Milica Radisic, Nilesh P. Talele, Xing-hua Wang, Boris Hinz, Armand Keating
    Abstract:

    MSCs are widely applied to regenerate heart Tissue in myocardial diseases but when grown in standard two-dimensional (2D) cultures exhibit limited potential for Cardiac repair and develop fibrogenic features with increasing culture time. MSCs can undergo partial cardiomyogenic differentiation, which improves their Cardiac repair capacity. When applied to collagen patches they may improve Cardiac Tissue Regeneration but the mechanisms remain elusive. Here, we investigated the regenerative properties of MSCs grown in a collagen scaffold as a three-dimensional (3D) culture system, and performed functional analysis using an engineered heart Tissue (EHT) model. We showed that the expression of cardiomyocyte-specific proteins by MSCs co-cultured with rat neonatal cardiomyocytes was increased in collagen patches versus conventional cultures. MSCs in 3D collagen patches were less fibrogenic, secreted more cardiotrophic factors, retained anti-apoptotic and immunomodulatory function, and responded less to TLR4 ligand lipopolysaccharide (LPS) stimulation. EHT analysis showed no effects by MSCs on cardiomyocyte function, whereas control dermal fibroblasts abrogated the beating of Cardiac Tissue constructs. We conclude that 3D collagen scaffold improves the cardioprotective effects of MSCs by enhancing the production of trophic factors and modifying their immune modulatory and fibrogenic phenotype. The improvement in myocardial function by MSCs after acquisition of a partial Cardiac cell-like phenotype is not due to enhanced MSC contractility. A better understanding of the mechanisms of MSC-mediated Tissue repair will help to further enhance the therapeutic potency of MSCs.

  • mesenchymal stromal cell therapy to promote Cardiac Tissue Regeneration and repair
    Current Opinion in Organ Transplantation, 2016
    Co-Authors: Agata Bartczak, Ian D Mcgilvray, Armand Keating
    Abstract:

    Purpose of review This review focuses on articles published from January 2015 to June 2016 on mesenchymal stromal cell (MSC) therapy for Cardiac Regeneration and repair. Recent findings During this period, reports published on MSCs address the best MSC Tissue source for cellular therapy, mechanisms of MSC activity and improving MSC longevity, and homing in vivo. Currently, there is no definitive therapeutic advantage of any one Tissue-derived MSC over another, and even combination therapies struggle with conflicting outcomes. MSC activity, persistence in vivo, or homing can be improved by priming strategies, genetic modification, or biomaterials. Despite numerous studies showing improvement in heart function after acute Cardiac injury, the reproducibility and efficacy of the therapy remains elusive and falls short of expectations in clinical trials. Although the safety of MSCs is undisputed, the success of MSC preparations in improving Cardiac function clinically remains uncertain due to challenges in correlating MSC potency with clinical outcomes, donor-related variation in MSC function, and a profusion of culture methodologies. Summary Several strategies are available to advance MSC cell therapy for acute Cardiac injury to promote Cardiac Regeneration and repair in rigorous preclinical and clinical studies.

Beth Schoen - One of the best experts on this subject based on the ideXlab platform.

  • electrospun extracellular matrix paving the way to tailor made natural scaffolds for Cardiac Tissue Regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native Tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the Tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate bioactive material for the Regeneration of different organs. Particularly for Cardiac Regeneration, ECM is studied as a patch and injectable scaffolds, which improve Cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine Cardiac ECM using electrospinning technology, is developed. This unique electrospun Cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of Cardiac ECM ,and ,above all, preserves key Cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural materials as tailor-made, well-defined structures.

  • Electrospun Extracellular Matrix: Paving the Way to Tailor‐Made Natural Scaffolds for Cardiac Tissue Regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native Tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the Tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate bioactive material for the Regeneration of different organs. Particularly for Cardiac Regeneration, ECM is studied as a patch and injectable scaffolds, which improve Cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine Cardiac ECM using electrospinning technology, is developed. This unique electrospun Cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of Cardiac ECM ,and ,above all, preserves key Cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural materials as tailor-made, well-defined structures.