The Experts below are selected from a list of 1341 Experts worldwide ranked by ideXlab platform

Nan Huang - One of the best experts on this subject based on the ideXlab platform.

  • the co deposition coating of collagen iv and laminin on hyaluronic acid pattern for better biocompatibility on Cardiovascular Biomaterials
    2020
    Co-Authors: Xiao Luo, Dan Zou, Congzhen Han, Ping Yang, An Sha Zhao, Lang Jiang, Pengyu Gao, Benli Yin, Nan Huang
    Abstract:

    Construct a coating to repair the endothelium function is the ordinary and effective method to get out of the troubles which introduced by the Cardiovascular implant devices. It indeed has plenty works on function construction which could inhibit the hyperplasia or accelerate the endothelialization with different functional proteins or molecules. However, a complete and healthy endothelium couldn't survive without the environment around. Thus, a logical biomimetic reconstruction with structure and function factors which using hyaluronic acid patterns to imitate the blood flow shear stress and co-depositing collagen type IV and laminin to achieve the biofunction of basement membrane had been proposed and realized in this work. After the tests of hemocompatibility, cytocompatibility and tissue compatibility, it had been indicated that this biomimetic coating could inhibit the adhesion of platelets, promote the proliferation and biofunction of endothelium cells, regulate smooth muscle cells with contractile phenotype and have much lower inflammatory response which might be a meaningful strategy on reconstruction and repairing of endothelium.

  • nature inspired extracellular matrix coating produced by micro patterned smooth muscle and endothelial cells endows Cardiovascular materials with better biocompatibility
    2019
    Co-Authors: Congzhen Han, Dan Zou, Xiao Luo, Ping Yang, Kun Zhang, Nan Huang
    Abstract:

    Functionalizing Cardiovascular Biomaterials with an extracellular matrix (ECM) via in vitro decellularization has been applied as an effective method to improve the biocompatibility of implants. However, the current ECM modified materials used for surface engineering implants have functional restrictions compared with the natural blood vessel due to distinguished cell phenotypes in vitro. Herein, we are inspired by the natural vascular basement membrane which is composed of an ECM secreted by physiological endothelial cells (EC) and smooth muscle cells (SMC), preparing a novel ECM coating by successive cell culture and decellularization: appropriately scaled hyaluronic acid (HA) micro-patterns are used to regulate the SMC phenotype to contraction and simulate the blood flow shear stress (BFSS) effect to control the EC physiological phenotype. The nature-inspired ECM coating significantly improves the material's hemocompatibility, cytocompatibility and tissue compatibility, and may be promising to break the function limitation of a single ECM and address more clinical complications.

  • preparation of a biomimetic ecm surface on Cardiovascular Biomaterials via a novel layer by layer decellularization for better biocompatibility
    2019
    Co-Authors: Dan Zou, Xiao Luo, Congzhen Han, Ping Yang, Nan Huang
    Abstract:

    Abstract Endothelial extracellular matrix (EC-ECM) modification by decellularization is generally recognized as an effective method for Cardiovascular Biomaterials to enhance their biocompatibility. However, the now available EC-ECM was mainly secreted by the in vitro cultured endothelial cells which lacked a physiological growth environment in vivo, such as blood flow shear stress (BFSS) acting, thus had a serious defect of biocompatibility. Our previous work markedly improved the biocompatibility of the EC-ECM modified materials by simulating the BFSS acting to control the endothelial cells with hyaluronic acid (HA) micro-pattern. In this contribution, the EC-ECM was further enriched onto the HA micro-pattern via a novel layer-by-layer decellularizatio method. In vitro platelets adhesion/activation, macrophages attachment test and ex vivo blood experiment of New Zealand White Rabbits suggested better blood compatibility and anti-inflammation property of this novel biomimetic ECM surface. The endothelial cells culture tests and in vivo rat subcutaneous implantation also proved its good pro-endothelialization function and tissue compatibility. In summary, the present study demonstrated better biocompatibility of the novel biomimetic ECM surface and its potential application for Cardiovascular Biomaterials modification.

  • controlling molecular weight of hyaluronic acid conjugated on amine rich surface toward better multifunctional Biomaterials for Cardiovascular implants
    2017
    Co-Authors: Jingan Li, Ping Yang, An Sha Zhao, Feng Wu, Kun Zhang, Zikun He, Nan Huang
    Abstract:

    The molecular weights (MWs) of hyaluronic acid (HA) in extracellular matrix secreted from both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs) play crucial roles in the Cardiovascular physiology, as HA with appropriate MW influences important pathways of Cardiovascular homeostasis, inhibits VSMC synthetic phenotype change and proliferation, inhibits platelet activation and aggregation, promotes endothelial monolayer repair and functionalization, and prevents inflammation and atherosclerosis. In this study, HA samples with gradients of MW (4 × 103, 1 × 105, and 5 × 105 Da) were prepared by covalent conjugation to a copolymerized film of polydopamine and hexamethylendiamine (PDA/HD) as multifunctional coatings (PDA/HD-HA) with potential to improve the biocompatibility of Cardiovascular Biomaterials. The coatings immobilized with high-MW-HA (PDA/HD-HA-2: 1 × 105 Da; PDA/HD-HA-3: 5 × 105 Da) exhibited a remarkable suppression of platelet activation/aggregation and thrombosis under 1...

  • responses of platelets and endothelial cells to heparin fibronectin complex on titanium in situ investigation by quartz crystal microbalance with dissipation and immunochemistry
    2013
    Co-Authors: Ping Yang, Nan Huang, Hongyan Ding
    Abstract:

    Platelet adhesion and endothelialization rates are frequently used to assess the biocompatibility of Biomaterials, which are crucial steps for the development of blood-contacting implanted devices. Co-immobilization of heparin and fibronectin (Hep/Fn) on titanium (Ti) surface has been proven to inhibit platelet adhesion and enhance endothelialization in our previous study, however, the interaction mechanisms of platelet and endothelial cell (EC) with biomolecules immobilized surface at the early stage are still not clear. In this study, the adhesion behavior of EC and platelet on biomolecules immobilized surface was evaluated using quartz crystal microbalance with dissipation (QCM-D) in real time and immunofluorescence/optical measurement. And the possible underlying mechanism was probed using immunochemistry. The results showed that EC underwent attachment and spreading process on Hep/Fn (pH 4) immobilized surface similar to that on bare Ti surface, while platelet displayed much larger activation on bare Ti surface than that on Hep/Fn (pH 4) immobilized surface. However, the adhesion behaviors of platelets and EC reflected in Df plots were different. The study brings forth the detailed interaction between heparin and fibronectin and the interaction of EC and platelet with the biomolecules coated surface, which will be helpful for better understanding the interaction mechanism of cell-Biomaterials interface and may potentially be useful for the development of new generation of Cardiovascular Biomaterials.

Ping Yang - One of the best experts on this subject based on the ideXlab platform.

  • the co deposition coating of collagen iv and laminin on hyaluronic acid pattern for better biocompatibility on Cardiovascular Biomaterials
    2020
    Co-Authors: Xiao Luo, Dan Zou, Congzhen Han, Ping Yang, An Sha Zhao, Lang Jiang, Pengyu Gao, Benli Yin, Nan Huang
    Abstract:

    Construct a coating to repair the endothelium function is the ordinary and effective method to get out of the troubles which introduced by the Cardiovascular implant devices. It indeed has plenty works on function construction which could inhibit the hyperplasia or accelerate the endothelialization with different functional proteins or molecules. However, a complete and healthy endothelium couldn't survive without the environment around. Thus, a logical biomimetic reconstruction with structure and function factors which using hyaluronic acid patterns to imitate the blood flow shear stress and co-depositing collagen type IV and laminin to achieve the biofunction of basement membrane had been proposed and realized in this work. After the tests of hemocompatibility, cytocompatibility and tissue compatibility, it had been indicated that this biomimetic coating could inhibit the adhesion of platelets, promote the proliferation and biofunction of endothelium cells, regulate smooth muscle cells with contractile phenotype and have much lower inflammatory response which might be a meaningful strategy on reconstruction and repairing of endothelium.

  • nature inspired extracellular matrix coating produced by micro patterned smooth muscle and endothelial cells endows Cardiovascular materials with better biocompatibility
    2019
    Co-Authors: Congzhen Han, Dan Zou, Xiao Luo, Ping Yang, Kun Zhang, Nan Huang
    Abstract:

    Functionalizing Cardiovascular Biomaterials with an extracellular matrix (ECM) via in vitro decellularization has been applied as an effective method to improve the biocompatibility of implants. However, the current ECM modified materials used for surface engineering implants have functional restrictions compared with the natural blood vessel due to distinguished cell phenotypes in vitro. Herein, we are inspired by the natural vascular basement membrane which is composed of an ECM secreted by physiological endothelial cells (EC) and smooth muscle cells (SMC), preparing a novel ECM coating by successive cell culture and decellularization: appropriately scaled hyaluronic acid (HA) micro-patterns are used to regulate the SMC phenotype to contraction and simulate the blood flow shear stress (BFSS) effect to control the EC physiological phenotype. The nature-inspired ECM coating significantly improves the material's hemocompatibility, cytocompatibility and tissue compatibility, and may be promising to break the function limitation of a single ECM and address more clinical complications.

  • preparation of a biomimetic ecm surface on Cardiovascular Biomaterials via a novel layer by layer decellularization for better biocompatibility
    2019
    Co-Authors: Dan Zou, Xiao Luo, Congzhen Han, Ping Yang, Nan Huang
    Abstract:

    Abstract Endothelial extracellular matrix (EC-ECM) modification by decellularization is generally recognized as an effective method for Cardiovascular Biomaterials to enhance their biocompatibility. However, the now available EC-ECM was mainly secreted by the in vitro cultured endothelial cells which lacked a physiological growth environment in vivo, such as blood flow shear stress (BFSS) acting, thus had a serious defect of biocompatibility. Our previous work markedly improved the biocompatibility of the EC-ECM modified materials by simulating the BFSS acting to control the endothelial cells with hyaluronic acid (HA) micro-pattern. In this contribution, the EC-ECM was further enriched onto the HA micro-pattern via a novel layer-by-layer decellularizatio method. In vitro platelets adhesion/activation, macrophages attachment test and ex vivo blood experiment of New Zealand White Rabbits suggested better blood compatibility and anti-inflammation property of this novel biomimetic ECM surface. The endothelial cells culture tests and in vivo rat subcutaneous implantation also proved its good pro-endothelialization function and tissue compatibility. In summary, the present study demonstrated better biocompatibility of the novel biomimetic ECM surface and its potential application for Cardiovascular Biomaterials modification.

  • controlling molecular weight of hyaluronic acid conjugated on amine rich surface toward better multifunctional Biomaterials for Cardiovascular implants
    2017
    Co-Authors: Jingan Li, Ping Yang, An Sha Zhao, Feng Wu, Kun Zhang, Zikun He, Nan Huang
    Abstract:

    The molecular weights (MWs) of hyaluronic acid (HA) in extracellular matrix secreted from both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs) play crucial roles in the Cardiovascular physiology, as HA with appropriate MW influences important pathways of Cardiovascular homeostasis, inhibits VSMC synthetic phenotype change and proliferation, inhibits platelet activation and aggregation, promotes endothelial monolayer repair and functionalization, and prevents inflammation and atherosclerosis. In this study, HA samples with gradients of MW (4 × 103, 1 × 105, and 5 × 105 Da) were prepared by covalent conjugation to a copolymerized film of polydopamine and hexamethylendiamine (PDA/HD) as multifunctional coatings (PDA/HD-HA) with potential to improve the biocompatibility of Cardiovascular Biomaterials. The coatings immobilized with high-MW-HA (PDA/HD-HA-2: 1 × 105 Da; PDA/HD-HA-3: 5 × 105 Da) exhibited a remarkable suppression of platelet activation/aggregation and thrombosis under 1...

  • responses of platelets and endothelial cells to heparin fibronectin complex on titanium in situ investigation by quartz crystal microbalance with dissipation and immunochemistry
    2013
    Co-Authors: Ping Yang, Nan Huang, Hongyan Ding
    Abstract:

    Platelet adhesion and endothelialization rates are frequently used to assess the biocompatibility of Biomaterials, which are crucial steps for the development of blood-contacting implanted devices. Co-immobilization of heparin and fibronectin (Hep/Fn) on titanium (Ti) surface has been proven to inhibit platelet adhesion and enhance endothelialization in our previous study, however, the interaction mechanisms of platelet and endothelial cell (EC) with biomolecules immobilized surface at the early stage are still not clear. In this study, the adhesion behavior of EC and platelet on biomolecules immobilized surface was evaluated using quartz crystal microbalance with dissipation (QCM-D) in real time and immunofluorescence/optical measurement. And the possible underlying mechanism was probed using immunochemistry. The results showed that EC underwent attachment and spreading process on Hep/Fn (pH 4) immobilized surface similar to that on bare Ti surface, while platelet displayed much larger activation on bare Ti surface than that on Hep/Fn (pH 4) immobilized surface. However, the adhesion behaviors of platelets and EC reflected in Df plots were different. The study brings forth the detailed interaction between heparin and fibronectin and the interaction of EC and platelet with the biomolecules coated surface, which will be helpful for better understanding the interaction mechanism of cell-Biomaterials interface and may potentially be useful for the development of new generation of Cardiovascular Biomaterials.

Daniel E Heath - One of the best experts on this subject based on the ideXlab platform.

  • nano scale clustering of integrin binding ligands regulates endothelial cell adhesion migration and endothelialization rate novel materials for small diameter vascular graft applications
    2017
    Co-Authors: Fatemeh Karimi, Andrea J Oconnor, Thomas G. Mckenzie, Greg G Qiao, Daniel E Heath
    Abstract:

    Blood contacting devices are commonly used in today's medical landscape. However, such devices (including small diameter vascular grafts) are limited by poor blood compatibility and may fail due to thrombosis. An attractive strategy for improving the blood compatibility of such devices is to generate Biomaterials that foster a confluent and functioning endothelial cell layer. Synthesizing materials that display integrin-binding peptide ligands is a common way to promote endothelialization. However, in addition to integrin-ligand binding, integrin clustering is necessary to achieve intracellular signaling events that influence cellular phenotype. In this study, we explored the impact of nano-scale clustering of integrin-binding ligands on endothelial cell functions by designing novel materials that promote the clustering of integrin receptors. RGD-functionalized copolymers were prepared via reversible addition–fragmentation chain transfer (RAFT) polymerization and used for the preparation of random and nano-clustered surfaces spanning a range of global and local RGD densities (global densities 0.4–1.9 μg of peptide per mg of polymer and local densities of 1–2.4 ligands per nano-cluster). The adhesion and migration of endothelial cells was improved on nano-clustered surfaces compared to random surfaces. The highest adhesion and migration speed of endothelial cells and the rapid development of the endothelial monolayer were observed on surfaces with the highest local and global peptide density. These results indicate that the nano-clustering of peptide ligands is a promising strategy for next generation Cardiovascular Biomaterials, especially for small diameter vascular graft applications where the development of a confluent and functioning endothelium holds the potential to prevent device failure due to thrombosis.

  • promoting endothelialization of polymeric Cardiovascular Biomaterials
    2017
    Co-Authors: Daniel E Heath
    Abstract:

    The lack of a blood compatible synthetic interface is one of the largest unaddressed challenges in the field of Biomaterials science. This technological shortcoming hinders the successful clinical application of small diameter vascular grafts and other Cardiovascular devices such as stents and artificial heart valves. Therefore, intensive research activities are ongoing to develop polymer materials with improved blood compatibility. One attractive strategy to improve the blood compatibility of an interface is to design surfaces that promote the development of an endothelium, the monolayer of endothelial cells that line our native vasculature and is responsible for blood compatibility. This article describes the recent strategies that have been used to generate polymeric materials that promote the development of an endothelium, discusses shortcomings in the field, and proposes future directions of research that can be undertaken to design next generation polymeric biomaterial that promote endothelialization. (Figure presented.).

Congzhen Han - One of the best experts on this subject based on the ideXlab platform.

  • the co deposition coating of collagen iv and laminin on hyaluronic acid pattern for better biocompatibility on Cardiovascular Biomaterials
    2020
    Co-Authors: Xiao Luo, Dan Zou, Congzhen Han, Ping Yang, An Sha Zhao, Lang Jiang, Pengyu Gao, Benli Yin, Nan Huang
    Abstract:

    Construct a coating to repair the endothelium function is the ordinary and effective method to get out of the troubles which introduced by the Cardiovascular implant devices. It indeed has plenty works on function construction which could inhibit the hyperplasia or accelerate the endothelialization with different functional proteins or molecules. However, a complete and healthy endothelium couldn't survive without the environment around. Thus, a logical biomimetic reconstruction with structure and function factors which using hyaluronic acid patterns to imitate the blood flow shear stress and co-depositing collagen type IV and laminin to achieve the biofunction of basement membrane had been proposed and realized in this work. After the tests of hemocompatibility, cytocompatibility and tissue compatibility, it had been indicated that this biomimetic coating could inhibit the adhesion of platelets, promote the proliferation and biofunction of endothelium cells, regulate smooth muscle cells with contractile phenotype and have much lower inflammatory response which might be a meaningful strategy on reconstruction and repairing of endothelium.

  • nature inspired extracellular matrix coating produced by micro patterned smooth muscle and endothelial cells endows Cardiovascular materials with better biocompatibility
    2019
    Co-Authors: Congzhen Han, Dan Zou, Xiao Luo, Ping Yang, Kun Zhang, Nan Huang
    Abstract:

    Functionalizing Cardiovascular Biomaterials with an extracellular matrix (ECM) via in vitro decellularization has been applied as an effective method to improve the biocompatibility of implants. However, the current ECM modified materials used for surface engineering implants have functional restrictions compared with the natural blood vessel due to distinguished cell phenotypes in vitro. Herein, we are inspired by the natural vascular basement membrane which is composed of an ECM secreted by physiological endothelial cells (EC) and smooth muscle cells (SMC), preparing a novel ECM coating by successive cell culture and decellularization: appropriately scaled hyaluronic acid (HA) micro-patterns are used to regulate the SMC phenotype to contraction and simulate the blood flow shear stress (BFSS) effect to control the EC physiological phenotype. The nature-inspired ECM coating significantly improves the material's hemocompatibility, cytocompatibility and tissue compatibility, and may be promising to break the function limitation of a single ECM and address more clinical complications.

  • preparation of a biomimetic ecm surface on Cardiovascular Biomaterials via a novel layer by layer decellularization for better biocompatibility
    2019
    Co-Authors: Dan Zou, Xiao Luo, Congzhen Han, Ping Yang, Nan Huang
    Abstract:

    Abstract Endothelial extracellular matrix (EC-ECM) modification by decellularization is generally recognized as an effective method for Cardiovascular Biomaterials to enhance their biocompatibility. However, the now available EC-ECM was mainly secreted by the in vitro cultured endothelial cells which lacked a physiological growth environment in vivo, such as blood flow shear stress (BFSS) acting, thus had a serious defect of biocompatibility. Our previous work markedly improved the biocompatibility of the EC-ECM modified materials by simulating the BFSS acting to control the endothelial cells with hyaluronic acid (HA) micro-pattern. In this contribution, the EC-ECM was further enriched onto the HA micro-pattern via a novel layer-by-layer decellularizatio method. In vitro platelets adhesion/activation, macrophages attachment test and ex vivo blood experiment of New Zealand White Rabbits suggested better blood compatibility and anti-inflammation property of this novel biomimetic ECM surface. The endothelial cells culture tests and in vivo rat subcutaneous implantation also proved its good pro-endothelialization function and tissue compatibility. In summary, the present study demonstrated better biocompatibility of the novel biomimetic ECM surface and its potential application for Cardiovascular Biomaterials modification.

Dan Zou - One of the best experts on this subject based on the ideXlab platform.

  • the co deposition coating of collagen iv and laminin on hyaluronic acid pattern for better biocompatibility on Cardiovascular Biomaterials
    2020
    Co-Authors: Xiao Luo, Dan Zou, Congzhen Han, Ping Yang, An Sha Zhao, Lang Jiang, Pengyu Gao, Benli Yin, Nan Huang
    Abstract:

    Construct a coating to repair the endothelium function is the ordinary and effective method to get out of the troubles which introduced by the Cardiovascular implant devices. It indeed has plenty works on function construction which could inhibit the hyperplasia or accelerate the endothelialization with different functional proteins or molecules. However, a complete and healthy endothelium couldn't survive without the environment around. Thus, a logical biomimetic reconstruction with structure and function factors which using hyaluronic acid patterns to imitate the blood flow shear stress and co-depositing collagen type IV and laminin to achieve the biofunction of basement membrane had been proposed and realized in this work. After the tests of hemocompatibility, cytocompatibility and tissue compatibility, it had been indicated that this biomimetic coating could inhibit the adhesion of platelets, promote the proliferation and biofunction of endothelium cells, regulate smooth muscle cells with contractile phenotype and have much lower inflammatory response which might be a meaningful strategy on reconstruction and repairing of endothelium.

  • nature inspired extracellular matrix coating produced by micro patterned smooth muscle and endothelial cells endows Cardiovascular materials with better biocompatibility
    2019
    Co-Authors: Congzhen Han, Dan Zou, Xiao Luo, Ping Yang, Kun Zhang, Nan Huang
    Abstract:

    Functionalizing Cardiovascular Biomaterials with an extracellular matrix (ECM) via in vitro decellularization has been applied as an effective method to improve the biocompatibility of implants. However, the current ECM modified materials used for surface engineering implants have functional restrictions compared with the natural blood vessel due to distinguished cell phenotypes in vitro. Herein, we are inspired by the natural vascular basement membrane which is composed of an ECM secreted by physiological endothelial cells (EC) and smooth muscle cells (SMC), preparing a novel ECM coating by successive cell culture and decellularization: appropriately scaled hyaluronic acid (HA) micro-patterns are used to regulate the SMC phenotype to contraction and simulate the blood flow shear stress (BFSS) effect to control the EC physiological phenotype. The nature-inspired ECM coating significantly improves the material's hemocompatibility, cytocompatibility and tissue compatibility, and may be promising to break the function limitation of a single ECM and address more clinical complications.

  • preparation of a biomimetic ecm surface on Cardiovascular Biomaterials via a novel layer by layer decellularization for better biocompatibility
    2019
    Co-Authors: Dan Zou, Xiao Luo, Congzhen Han, Ping Yang, Nan Huang
    Abstract:

    Abstract Endothelial extracellular matrix (EC-ECM) modification by decellularization is generally recognized as an effective method for Cardiovascular Biomaterials to enhance their biocompatibility. However, the now available EC-ECM was mainly secreted by the in vitro cultured endothelial cells which lacked a physiological growth environment in vivo, such as blood flow shear stress (BFSS) acting, thus had a serious defect of biocompatibility. Our previous work markedly improved the biocompatibility of the EC-ECM modified materials by simulating the BFSS acting to control the endothelial cells with hyaluronic acid (HA) micro-pattern. In this contribution, the EC-ECM was further enriched onto the HA micro-pattern via a novel layer-by-layer decellularizatio method. In vitro platelets adhesion/activation, macrophages attachment test and ex vivo blood experiment of New Zealand White Rabbits suggested better blood compatibility and anti-inflammation property of this novel biomimetic ECM surface. The endothelial cells culture tests and in vivo rat subcutaneous implantation also proved its good pro-endothelialization function and tissue compatibility. In summary, the present study demonstrated better biocompatibility of the novel biomimetic ECM surface and its potential application for Cardiovascular Biomaterials modification.