The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Andreas Lendlein - One of the best experts on this subject based on the ideXlab platform.
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Evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
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evaluating Polymeric Biomaterial environment interfaces by langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
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the influence of a multifunctional Polymeric Biomaterial on the concentration of acute phase proteins in an animal model
Clinical Hemorheology and Microcirculation, 2007Co-Authors: D Rickert, M O Scheithauer, Saadet Coskun, Steffen Kelch, Andreas Lendlein, R P FrankeAbstract:The concentrations of the acute phase proteins α1-Acid Glycoprotein (AAG) and haptoglobin were determined in Sprague-Dawley-rats after implantation of a novel biodegradable multifunctional Polymeric Biomaterial for the reconstruction of a gastric wall defect (polymer group; n = 42). For comparison, the concentrations of AAG and haptoglobin were measured as well after primary wound closure of the gastric wall defect without Biomaterial implantation (control group; n = 21) and in rats without any surgical procedure (baseline group; n = 21). The implantation periods were 1 week, 4 weeks and 6 months. The concentrations of AAG and haptoglobin were measured by an ELISA assay. Gastrointestinal complications like fistula, perforation or peritonitis did not occur in any of the animals. No statistically significant differences in the concentrations of AAG and haptoglobin were detected between the polymer and the control group. An adequate mechanical stability of the Polymeric Biomaterial was detectable under the extreme pathophysiological conditions of the stomach milieu. In further examinations the correlation between the intraperitoneal cytokine levels of the animals and the following systemic inflammatory markers should be analysed. Further investigations are needed to analyse the mechanisms of the tissue integration of a Biomaterial as well as the process of the tissue remodeling and the influence of the immune system on these mechanisms. The knowledge of these processes is necessary to adapt the multifunctional Biomaterial and prepare it thus for the use and implantation in different body locations and to develop novel therapeutical options in medicine.
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Polymeric Biomaterials in head and neck surgery: first results of biocompatibility testing of a degradable polymer in an animal model
Laryngo- rhino- otologie, 2007Co-Authors: D Rickert, Saadet Coskun, Steffen Kelch, Andreas Lendlein, M O ScheithauerAbstract:BACKGROUND: Novel, multifunctional Polymeric Biomaterials offer a highly specific adjustment to the physiological, anatomical and surgical requirements and can thereby facilitate new therapeutical options in head and neck surgery. Reconstructing defect mucosal regions using an appropriate Biomaterial would be an improved therapy for salivary fistulas. METHODS: An elastic, long term degradable Polymeric Biomaterial was investigated in a randomized, prospective animal study. 12 Sprague-Dawley rats were used for polymer implantation, 6 animals underwent a sham operation. A standardized round gastrotomy was performed ventrally. The defect of the gastric wall was closed with an elastic copolymer network (polymer group). In the control group a primary wound closure without Biomaterial implantation was performed. The period of implantation time was 7, 14 and 30 days. The impermeability was studied by using a pressure-measuring probe. RESULTS: No gastrointestinal complications like fistula or peritonitis occurred after the polymer implantation. Despite maximum stretching of the gastric wall, no leakage was detected. In both groups, macroscopically and histologically a regular wound healing at the serosal and mucosal site was found. CONCLUSIONS: The chemical and hydrolytic stability and the biomechanical behavior of the copolymer network during the implantation time period were well adjusted to the conditions of the stomach. As gastric acid and mobility of the implantation site are a "worst case scenario” for implants, it can be anticipated, that the Biomaterial will also work in the area of the upper aerodigestive tract.
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expression of mmps and timps in primary epithelial cell cultures of the upper aerodigestive tract seeded on the surface of a novel Polymeric Biomaterial
Clinical Hemorheology and Microcirculation, 2005Co-Authors: D Rickert, Steffen Kelch, Andreas Lendlein, Marsha A. Moses, R P FrankeAbstract:Introduction: Using standard cell biological and biochemical experimental approaches we were able to test the ability of a particular polymer construct to support the adhesion, proliferation, and the cellular acitivity of pharyngeal cells. The delicate balance between Matrix Metalloproteinases (MMPs) and their endogenous inhibitors (Tissue Inhibitor of MMPs, TIMPs) have a decisive function in the remodeling of the extracellular matrix during cellular ingrowth. Novel Polymeric Biomaterials may be useful to develop new therapeutic options in head and neck surgery. Methods: Primary cell cultures of the pharynx of Sprague- Dawley rats were seeded on the surface of a thermoplastic multi-block copolymer and on a polystyrene surface as control. Conditioned media of the primary cells was analyzed for MMPs and TIMPs. The MMP and TIMP expression was analysed by zymography and a radiometric enzyme assay. Results: No statistically significant differences in the levels of MMP-1, MMP-2, MMP-9 and TIMPs were detected between cells grown on the novel polymer surface versus control. Conclusion: An appropriate understanding of the molecular machinery that regulates gene expression and cellular growth in tissue engineered contructs is the requirement for an optimal adaptation of biodegradable Biomaterials to develop new therapeutic options in otolaryngology and head and neck surgery.
Thomas J Webster - One of the best experts on this subject based on the ideXlab platform.
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An In Vitro Study of Nano-fiber Polymers for Guided Vascular Regeneration
MRS Online Proceedings Library, 2011Co-Authors: Derick C Miller, Anil Thapa, Karen M Haberstroh, Thomas J WebsterAbstract:Biomaterials that successfully integrate into surrounding tissue should match not only the tissue’s mechanical properties, but also the dimensions of the associated nano-structured extra-cellular matrix (ECM) components. The goal of this research was to use these ideals to develop a synthetic, nano-structured, Polymeric Biomaterial that has cytocompatible and mechanical behaviors similar to that of natural vascular tissue. In a novel manner, poly-lactic acid/polyglycolic acid (PLGA) (50/50 wt.% mix) and polyurethane were separately synthesized to possess a range of fiber dimensions in the micron and nanometer regime. Preliminary results indicated that decreasing fiber diameter on both PLGA and PU enhanced arterial smooth muscle cell adhesion; specifically, arterial smooth muscle cell adhesion increased 23% when PLGA fiber dimensions decreased from 500 to 50 nm and increased 76% on nano-structured, compared to conventional structured, polyurethane. However, nano-structured PLGA decreased endothelial cell adhesion by 52%, whereas adhesion of these same cells was increased by 50% on polyurethane. For these reasons, the present in vitro study provides the first evidence that polymer fiber dimensions can be used to selectively control cell functions for vascular prosthesis.
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Decreased Fibroblast and Increased Osteoblast Functions on Ionic Plasma Deposited Nanostructured Ti Coatings
Nanoscale Research Letters, 2007Co-Authors: Ariel Cohen, Peishan Liu-synder, Dan Storey, Thomas J WebsterAbstract:Bioactive coatings are in high demand to control cellular functions for numerous medical devices. The objective of this in vitro study was to characterize for the first time fibroblast (fibrous scar tissue forming cells) adhesion and proliferation on an important Polymeric Biomaterial (silicone) coated with titanium using a novel ionic plasma deposition (IPD) process. Fibroblasts are one of the first anchorage-dependent cells to arrive at an implant surface during the wound healing process. Persistent excessive functions of fibroblasts have been linked to detrimental fibrous tissue formation which may cause implant failure. The IPD process creates a surface-engineered nanostructure (with features usually below 100 nm) by first using a vacuum to remove all contaminants, then guiding charged metallic ions or plasma to the surface of a medical device at ambient temperature. Results demonstrated that compared to currently used titanium and uncoated silicone, silicone coated with titanium using IPD significantly decreased fibroblast adhesion and proliferation. Results also showed competitively increased osteoblast (bone-forming cells) over fibroblast adhesion on silicone coated with titanium; in contrast, osteoblast adhesion was not competitively increased over fibroblast adhesion on uncoated silicone or titanium controls. In this manner, this study strongly suggests that IPD should be further studied for Biomaterial applications in which fibrous tissue encapsulation is undesirable (such as for orthopedic implants, cardiovascular components, etc.).
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endothelial and vascular smooth muscle cell function on poly lactic co glycolic acid with nano structured surface features
Biomaterials, 2004Co-Authors: Derick C Miller, Anil Thapa, Karen M Haberstroh, Thomas J WebsterAbstract:Abstract Biomaterials that successfully integrate into surrounding tissue should match not only the tissue's mechanical properties, but also its topography. The cellular response to a Biomaterial may be enhanced in synthetic polymer formulations by mimicking the surface roughness created by the associated nano-structured extra-cellular matrix components of natural tissue. As a first step towards this endeavor, the goal of the present in vitro study was to use these design parameters to develop a synthetic, nano-structured, Polymeric Biomaterial that promotes cell adhesion and growth for vascular applications. In a novel manner, poly(lactic-co-glycolic acid) (PLGA) (50/50 wt% mix) was synthesized to possess a range (from micron to nanometer) of surface features. Reduction of surface features was accomplished by treating conventional PLGA with various concentrations of NaOH for select periods of time. Results from cell experiments indicated that, compared to conventional PLGA, NaOH treated PLGA enhanced vascular smooth muscle cell adhesion and proliferation. However, PLGA prepared by soaking in NaOH decreased endothelial cell adhesion and proliferation compared to conventional PLGA. After further investigation, this finding was determined to be a result of chemical (and not topographical) changes during polymer synthesis. Surface chemistry effects were removed while retaining nano-structured topography by using polymer/elastomer casting methods. Results demonstrated that endothelial and smooth muscle cell densities increased on nano-structured cast PLGA. For these reasons, the present in vitro study provided the first evidence that nano-structured surface features can significantly improve vascular cell densities; such design criteria can be used in the synthesis of the next-generation of more successful tissue-engineered vascular grafts.
Anne-christin Schöne - One of the best experts on this subject based on the ideXlab platform.
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Evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
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evaluating Polymeric Biomaterial environment interfaces by langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
Toralf Roch - One of the best experts on this subject based on the ideXlab platform.
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Evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
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evaluating Polymeric Biomaterial environment interfaces by langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
Burkhard Schulz - One of the best experts on this subject based on the ideXlab platform.
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Evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.
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evaluating Polymeric Biomaterial environment interfaces by langmuir monolayer techniques
Journal of the Royal Society Interface, 2017Co-Authors: Anne-christin Schöne, Toralf Roch, Burkhard Schulz, Andreas LendleinAbstract:Polymeric Biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of Biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal in vivo performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating Polymeric Biomaterial–environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their in vivo behaviour.