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Gladwin S. Das - One of the best experts on this subject based on the ideXlab platform.

  • the development of porous alginate elastin peg composite matrix for Cardiovascular Engineering
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

  • The Development of Porous Alginate/Elastin/PEG Composite Matrix for Cardiovascular Engineering:
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

Thomas Chandy - One of the best experts on this subject based on the ideXlab platform.

  • the development of porous alginate elastin peg composite matrix for Cardiovascular Engineering
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

  • The Development of Porous Alginate/Elastin/PEG Composite Matrix for Cardiovascular Engineering:
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

Gundu H.r. Rao - One of the best experts on this subject based on the ideXlab platform.

  • the development of porous alginate elastin peg composite matrix for Cardiovascular Engineering
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

  • The Development of Porous Alginate/Elastin/PEG Composite Matrix for Cardiovascular Engineering:
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

Robert F. Wilson - One of the best experts on this subject based on the ideXlab platform.

  • the development of porous alginate elastin peg composite matrix for Cardiovascular Engineering
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

  • The Development of Porous Alginate/Elastin/PEG Composite Matrix for Cardiovascular Engineering:
    Journal of Biomaterials Applications, 2003
    Co-Authors: Thomas Chandy, Gundu H.r. Rao, Robert F. Wilson, Gladwin S. Das
    Abstract:

    The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue Engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela : PEG (7 : 3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 � C and � 20 � C had a mean surface pore size of 35-45mm. However, their ultrastructures had shown bigger pore structures (60-75mm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 � C had larger ultrastructural pores than that of membranes prepared at � 20 � C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 � C was higher with Alg/Ela/PEG (61.6 � 4.8%), compared to Alg/Ela (49.9 � 0.3%). The enzymatic

Xiongbiao Chen - One of the best experts on this subject based on the ideXlab platform.

  • Optimization of nanoparticles for Cardiovascular tissue Engineering
    Nanotechnology, 2015
    Co-Authors: Mohammad Izadifar, Michael Kelly, Azita Haddadi, Xiongbiao Chen
    Abstract:

    Nano-particulate delivery systems have increasingly been playing important roles in Cardiovascular tissue Engineering. Properties of nanoparticles (e.g. size, polydispersity, loading capacity, zeta potential, morphology) are essential to system functions. Notably, these characteristics are regulated by fabrication variables, but in a complicated manner. This raises a great need to optimize fabrication process variables to ensure the desired nanoparticle characteristics. This paper presents a comprehensive experimental study on this matter, along with a novel method, the so-called Geno-Neural approach, to analyze, predict and optimize fabrication variables for desired nanoparticle characteristics. Specifically, ovalbumin was used as a protein model of growth factors used in Cardiovascular tissue regeneration, and six fabrication variables were examined with regard to their influence on the characteristics of nanoparticles made from high molecular weight poly(lactide-co-glycolide). The six-factor five-level central composite rotatable design was applied to the conduction of experiments, and based on the experimental results, a geno-neural model was developed to determine the optimum fabrication conditions. For desired particle sizes of 150, 200, 250 and 300 nm, respectively, the optimum conditions to achieve the low polydispersity index, higher negative zeta potential and higher loading capacity were identified based on the developed geno-neural model and then evaluated experimentally. The experimental results revealed that the polymer and the external aqueous phase concentrations and their interactions with other fabrication variables were the most significant variables to affect the size, polydispersity index, zeta potential, loading capacity and initial burst release of the nanoparticles, while the electron microscopy images of the nanoparticles showed their spherical geometries with no sign of large pores or cracks on their surfaces. The release study revealed that the onset of the third phase of release can be affected by the polymer concentration. Circular dichroism spectroscopy indicated that ovalbumin structural integrity is preserved during the encapsulation process. Findings from this study would greatly contribute to the design of high molecular weight poly(lactide-co-glycolide) nanoparticles for prolonged release patterns in Cardiovascular Engineering.

  • Engineering Angiogenesis for Myocardial Infarction Repair: Recent Developments, Challenges, and Future Directions
    Cardiovascular Engineering and Technology, 2014
    Co-Authors: Mohammad Izadifar, Michael E. Kelly, Xiongbiao Chen
    Abstract:

    Myocardial infarction is a Cardiovascular disorder that remains a critical health issue worldwide. Because of its intrinsic inability to regenerate, cardiac tissue fails to repair itself after myocardial infarction. Cardiovascular Engineering is a promising approach to regenerating myocardium for myocardial infarction repair. Despite explosive growth in research and interest in this field, Cardiovascular regenerative medicine faces many challenges, with the need for rapid vascularization being the most pressing. Due to the high metabolic demand of cardiac cells, myocytes transplanted or implanted via cardiac scaffolds in the infarcted region do not survive without the timely formation of a microvascular network in the infarcted area or within the scaffolds. To address this issue, various strategies have been developed based on angiogenesis stimulation, prevascularization, and inosculation to promote microvascular network formation within the cardiac scaffolds. This paper describes cardiac tissue Engineering strategies, key challenges in Cardiovascular regenerative medicine, and various vascularization strategies, with an aim to review recent advances and developments in Engineering vascularization and inosculation approaches towards the rapid integration of cardiac scaffolds, once implanted, with the host tissue. Challenges in Engineering angiogenesis and future directions to address the issue of rapid inosculation are also discussed.