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  • Retinal projections in the short-tailed fruit bat, Carollia perspicillata, as studied using the axonal transport of cholera toxin B subunit: Comparison with mouse.
    The Journal of comparative neurology, 2015
    Co-Authors: Frank Scalia, John J Rasweiler, John Danias
    Abstract:

    To provide a modern description of the Chiropteran visual system, the subcortical retinal projections were studied in the short-tailed fruit bat, Carollia perspicillata, using the anterograde transport of eye-injected cholera toxin B subunit, supplemented by the silver-impregnation of anterograde degeneration following eye removal, and compared with the retinal projections of the mouse. The retinal projections were heavily labeled by the transported toxin in both species. Almost all components of the murine retinal projection are present in Carollia in varying degrees of prominence and laterality. The projections: to the superior colliculus, accessory optic nuclei, and nucleus of the optic tract are predominantly or exclusively contralateral; to the dorsal lateral geniculate nucleus and posterior pretectal nucleus are predominantly contralateral; to the ventral lateral geniculate nucleus, intergeniculate leaflet, and olivary pretectal nucleus have a substantial ipsilateral component; and to the suprachiasmatic nucleus are symmetrically bilateral. The retinal projection in Carollia is surprisingly reduced at the anterior end of the dorsal lateral geniculate and superior colliculus, suggestive of a paucity of the relevant ganglion cells in the ventrotemporal retina. In the superior colliculus, in which the superficial gray layer is very thin, the projection is patchy in places where the layer is locally absent. Except for a posteriorly located lateral terminal nucleus, the other accessory optic nuclei are diminutive in Carollia, as is the nucleus of the optic tract. In both species the cholera toxin labeled sparse groups of apparently terminating axons in numerous regions not listed above. A question of their significance is discussed.

  • Isolation, genomic structure and developmental expression of Fgf8 in the short-tailed fruit bat, Carollia perspicillata
    The International journal of developmental biology, 2007
    Co-Authors: Chris J Cretekos, John J Rasweiler, Jian Min Deng, Eric D. Green, Richard R Behringer
    Abstract:

    Fibroblast growth factor-8 (Fgf8) encodes a secreted protein which was initially identified as the factor responsible for androgen-dependant growth of mouse mammary carcinoma cells (Tanaka et al., 1992). Fgf8 has been subsequently implicated in the patterning and growth of the gastrulating embryo, paraxial mesoderm (somites), limbs, craniofacial tissues, central nervous system and other organ systems during the development of several vertebrate model animals. Consistent with these findings, Fgf8 is expressed in a complex and dynamic pattern during vertebrate embryogenesis. Here we report the isolation and characterization of a bat (Carollia perspicillata) Fgf8 orthologue. Compared with those of other model vertebrates, Carollia Fgf8 is conserved with respect to genomic structure, sequence and many domains of developmental expression pattern. Interestingly, the expression domain marking the apical ectodermal ridge of the developing limb shows a striking difference compared to that of mouse, consistent with evolutionary diversification of bat limb morphology.

  • embryonic staging system for the short tailed fruit bat Carollia perspicillata a model organism for the mammalian order chiroptera based upon timed pregnancies in captive bred animals
    Developmental Dynamics, 2005
    Co-Authors: Chris J Cretekos, Scott D Weatherbee, Chih Hsin Chen, Nilima K Badwaik, Lee Niswander, Richard R Behringer, John J Rasweiler
    Abstract:

    There are approximately 4,800 extant species of mammals that exhibit tremendous morphological, physiological, and developmental diversity. Yet embryonic development has been studied in only a few mammalian species. Among mammals, bats are second only to rodents with regard to species number and habitat range and are the most abundant mammals in undisturbed tropical regions. Bat development, though, remains relatively unstudied. Here, we describe and illustrate a staging series of embryonic development for the short-tailed fruit bat, Carollia perspicillata, based on embryos collected at timed intervals after captive matings. As Carollia can be readily maintained and propagated in captivity and is extremely abundant in the wild, it offers an attractive choice as a chiropteran model organism. This staging system provides a framework for studying Carollia embryogenesis and should prove useful as a guide for embryological studies of other bat species and for comparisons with other orders of mammals.

  • embryonic staging system for the short tailed fruit bat Carollia perspicillata a model organism for the mammalian order chiroptera based upon timed pregnancies in captive bred animals
    Developmental Dynamics, 2005
    Co-Authors: Chris J Cretekos, Scott D Weatherbee, Chih Hsin Chen, Nilima K Badwaik, Lee Niswander, Richard R Behringer, John J Rasweiler
    Abstract:

    There are approximately 4,800 extant species of mammals that exhibit tremendous morphological, physiological, and developmental diversity. Yet embryonic development has been studied in only a few mammalian species. Among mammals, bats are second only to rodents with regard to species number and habitat range and are the most abundant mammals in undisturbed tropical regions. Bat development, though, remains relatively unstudied. Here, we describe and illustrate a staging series of embryonic development for the short-tailed fruit bat, Carollia perspicillata, based on embryos collected at timed intervals after captive matings. As Carollia can be readily maintained and propagated in captivity and is extremely abundant in the wild, it offers an attractive choice as a chiropteran model organism. This staging system provides a framework for studying Carollia embryogenesis and should prove useful as a guide for embryological studies of other bat species and for comparisons with other orders of mammals. Developmental Dynamics 233:721–738, 2005. © 2005 Wiley-Liss, Inc.

  • Unusual patterns of intermediate filament protein expression by the trophoblast and decidual cells of the short-tailed fruit bat, Carollia perspicillata
    Placenta, 2000
    Co-Authors: John J Rasweiler, Nilima K Badwaik, T.a. Sugarbaker
    Abstract:

    In the short-tailed fruit bat (Carollia perspicillata) pregnancy can be prolonged by the occurrence of lengthy delays after implantation. This is associated with the development of highly invasive trophoblast that can penetrate the myometrium, mesenteries of the reproductive tract and the oviducts via perivascular (interstitial) routes. In order to confirm the identity and distribution of this trophoblast, intermediate filament protein immunocytochemistry was utilized. In some respects the expression of these proteins differed from what has been reported for more commonly-studied species. Cytotrophoblast in the placenta, its cytotrophoblastic shell and the highly invasive trophoblast strongly expressed cytokeratins. As pregnancy progressed, however, cytokeratin expression by syncytiotrophoblast lining much of the placental labyrinth became very weak. The cytotrophoblastic shell and highly invasive trophoblast also expressed vimentin. The highly invasive trophoblast was unusual in that it developed dendritic processes that sometimes extended out into adjacent tissues in great profusion. Decidual cells generally expressed desmin and vimentin; however, some also coexpressed cytokeratins. These observations indicate that some of the trophoblast in Carollia undergoes a significant epithelial-mesenchymal transformation. They also suggest that caution should be exercised in relying upon intermediate filament proteins as markers for cell identification purposes in exotic species, or when the patterns of protein expression by fetal and maternal cells might be altered in pathological or experimental situations.

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