The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Gérald Pierard - One of the best experts on this subject based on the ideXlab platform.
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Skin Diseases associated with Bartonella infection: Facts and controversies
Clinics in dermatology, 2010Co-Authors: Claudine Piérard-franchimont, Pascale Quatresooz, Gérald PierardAbstract:The genus Bartonella is composed of a series of species and subspecies. Ten of them are responsible for human infections. The best-identified Diseases are cat scratch Disease (B henselae and possibly B clarridgeiae), trench fever (B quintana), bacillary angiomatosis (B quintana and B henselae), and the spectrum of verruga peruana, Carrion Disease, and Oroya fever (B bacilliformis). Controversies exist about the implication of a few other microorganisms being involved in these Diseases. Several other conditions have been associated with the presence of Bartonella spp, but these observations await confirmation.
Claudine Piérard-franchimont - One of the best experts on this subject based on the ideXlab platform.
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Skin Diseases associated with Bartonella infection: Facts and controversies
Clinics in dermatology, 2010Co-Authors: Claudine Piérard-franchimont, Pascale Quatresooz, Gérald PierardAbstract:The genus Bartonella is composed of a series of species and subspecies. Ten of them are responsible for human infections. The best-identified Diseases are cat scratch Disease (B henselae and possibly B clarridgeiae), trench fever (B quintana), bacillary angiomatosis (B quintana and B henselae), and the spectrum of verruga peruana, Carrion Disease, and Oroya fever (B bacilliformis). Controversies exist about the implication of a few other microorganisms being involved in these Diseases. Several other conditions have been associated with the presence of Bartonella spp, but these observations await confirmation.
Pascale Quatresooz - One of the best experts on this subject based on the ideXlab platform.
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Skin Diseases associated with Bartonella infection: Facts and controversies
Clinics in dermatology, 2010Co-Authors: Claudine Piérard-franchimont, Pascale Quatresooz, Gérald PierardAbstract:The genus Bartonella is composed of a series of species and subspecies. Ten of them are responsible for human infections. The best-identified Diseases are cat scratch Disease (B henselae and possibly B clarridgeiae), trench fever (B quintana), bacillary angiomatosis (B quintana and B henselae), and the spectrum of verruga peruana, Carrion Disease, and Oroya fever (B bacilliformis). Controversies exist about the implication of a few other microorganisms being involved in these Diseases. Several other conditions have been associated with the presence of Bartonella spp, but these observations await confirmation.
Chien Chung Chao - One of the best experts on this subject based on the ideXlab platform.
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Rapid and Sensitive Detection of Bartonella bacilliformis in Experimentally Infected Sand Flies by Loop-Mediated Isothermal Amplification (LAMP) of the Pap31 Gene
2016Co-Authors: Nasikarn Angkasekwinai, Erin H. Atkins, Richard N. Johnson, John P. Grieco, Wei Mei Ching, Chien Chung ChaoAbstract:Background: Carrion ’ Disease, caused by Bartonella bacilliformis, remains truly neglected due to its focal geographical nature. A wide spectrum of clinical manifestations, including asymptomatic bacteremia, and lack of a sensitive diagnostic test can potentially lead to a spread of the Disease into non-endemic regions where competent sand fly vectors may be present. A reliable test capable of detecting B. bacilliformis is urgently needed. Our objective is to develop a loop-mediated isothermal amplification (LAMP) assay targeting the pap31 gene to detect B. bacilliformis. Methods and Findings: The sensitivity of the LAMP was evaluated in comparison to qPCR using plasmid DNA containing the target gene and genomic DNA in the absence and presence of human or sand fly DNA. The detection limit of LAMP was 1 to 10 copies/mL, depending on the sample metrics. No cross-reaction was observed when testing against a panel of various closely related bacteria. The utility of the LAMP was further compared to qPCR by the examination of 74 Lutzomyia longipalpis sand flies artificially fed on blood spiked with B. bacilliformis and harvested at days (D) 1, 3, 5, 7 and 9 post feeding. Only 86 % of sand flies at D1 and 63 % of flies at D3 were positive by qPCR. LAMP was able to detect B. bacilliformis in all those flies confirmed positive by qPCR. However, none of the flies after D3 were positive by either LAMP or qPCR. In addition to demonstrating the sensitivity of the LAMP assay, these results suggest that B. bacilliformis cannot propagate in artificially fed L. longipalpis
Nasikarn Angkasekwinai - One of the best experts on this subject based on the ideXlab platform.
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Rapid and Sensitive Detection of Bartonella bacilliformis in Experimentally Infected Sand Flies by Loop-Mediated Isothermal Amplification (LAMP) of the Pap31 Gene
2016Co-Authors: Nasikarn Angkasekwinai, Erin H. Atkins, Richard N. Johnson, John P. Grieco, Wei Mei Ching, Chien Chung ChaoAbstract:Background: Carrion ’ Disease, caused by Bartonella bacilliformis, remains truly neglected due to its focal geographical nature. A wide spectrum of clinical manifestations, including asymptomatic bacteremia, and lack of a sensitive diagnostic test can potentially lead to a spread of the Disease into non-endemic regions where competent sand fly vectors may be present. A reliable test capable of detecting B. bacilliformis is urgently needed. Our objective is to develop a loop-mediated isothermal amplification (LAMP) assay targeting the pap31 gene to detect B. bacilliformis. Methods and Findings: The sensitivity of the LAMP was evaluated in comparison to qPCR using plasmid DNA containing the target gene and genomic DNA in the absence and presence of human or sand fly DNA. The detection limit of LAMP was 1 to 10 copies/mL, depending on the sample metrics. No cross-reaction was observed when testing against a panel of various closely related bacteria. The utility of the LAMP was further compared to qPCR by the examination of 74 Lutzomyia longipalpis sand flies artificially fed on blood spiked with B. bacilliformis and harvested at days (D) 1, 3, 5, 7 and 9 post feeding. Only 86 % of sand flies at D1 and 63 % of flies at D3 were positive by qPCR. LAMP was able to detect B. bacilliformis in all those flies confirmed positive by qPCR. However, none of the flies after D3 were positive by either LAMP or qPCR. In addition to demonstrating the sensitivity of the LAMP assay, these results suggest that B. bacilliformis cannot propagate in artificially fed L. longipalpis