The Experts below are selected from a list of 1380 Experts worldwide ranked by ideXlab platform
Claudio Migliaresi - One of the best experts on this subject based on the ideXlab platform.
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novel genipin cross linked chitosan silk fibroin sponges for Cartilage Engineering strategies
Biomacromolecules, 2008Co-Authors: Simone Santos Silva, Manuela E Gomes, Rui L. Reis, João F. Mano, Antonella Motta, Marcia T Rodrigues, Ana F M Pinheiro, Claudio MigliaresiAbstract:The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural Cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from α-helix/random-coil to β-sheet structure, distinct surface morphologies, and pH/swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges’ potential in Cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cell...
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Novel Genipin-Cross-Linked Chitosan/Silk Fibroin Sponges for Cartilage Engineering Strategies
Biomacromolecules, 2008Co-Authors: Simone Santos Silva, Manuela E Gomes, Rui L. Reis, João F. Mano, Antonella Motta, Marcia T Rodrigues, Ana F M Pinheiro, Claudio MigliaresiAbstract:The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural Cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from α-helix/random-coil to β-sheet structure, distinct surface morphologies, and pH/swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges’ potential in Cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cell...
Rui L. Reis - One of the best experts on this subject based on the ideXlab platform.
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From nano- to macro-scale: nanotechnology approaches for spatially controlled delivery of bioactive factors for bone and Cartilage Engineering
Nanomedicine, 2012Co-Authors: Vitor E Santo, João Filipe Mano, Manuela E Gomes, Rui L. ReisAbstract:The field of biomaterials has advanced towards the molecular and nanoscale design of bioactive systems for tissue Engineering, regenerative medicine and drug delivery. Spatial cues are displayed in the 3D extracellular matrix and can include signaling gradients, such as those observed during chemotaxis. Architectures range from the nanometer to the centimeter length scales as exemplified by extracellular matrix fibers, cells and macroscopic shapes. The main focus of this review is the application of a biomimetic approach by the combination of architectural cues, obtained through the application of micro- and nanofabrication techniques, with the ability to sequester and release growth factors and other bioactive agents in a spatiotemporal controlled manner for bone and Cartilage Engineering.
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novel genipin cross linked chitosan silk fibroin sponges for Cartilage Engineering strategies
Biomacromolecules, 2008Co-Authors: Simone Santos Silva, Manuela E Gomes, Rui L. Reis, João F. Mano, Antonella Motta, Marcia T Rodrigues, Ana F M Pinheiro, Claudio MigliaresiAbstract:The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural Cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from α-helix/random-coil to β-sheet structure, distinct surface morphologies, and pH/swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges’ potential in Cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cell...
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Novel Genipin-Cross-Linked Chitosan/Silk Fibroin Sponges for Cartilage Engineering Strategies
Biomacromolecules, 2008Co-Authors: Simone Santos Silva, Manuela E Gomes, Rui L. Reis, João F. Mano, Antonella Motta, Marcia T Rodrigues, Ana F M Pinheiro, Claudio MigliaresiAbstract:The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural Cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from α-helix/random-coil to β-sheet structure, distinct surface morphologies, and pH/swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges’ potential in Cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cell...
Manuela E Gomes - One of the best experts on this subject based on the ideXlab platform.
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From nano- to macro-scale: nanotechnology approaches for spatially controlled delivery of bioactive factors for bone and Cartilage Engineering
Nanomedicine, 2012Co-Authors: Vitor E Santo, João Filipe Mano, Manuela E Gomes, Rui L. ReisAbstract:The field of biomaterials has advanced towards the molecular and nanoscale design of bioactive systems for tissue Engineering, regenerative medicine and drug delivery. Spatial cues are displayed in the 3D extracellular matrix and can include signaling gradients, such as those observed during chemotaxis. Architectures range from the nanometer to the centimeter length scales as exemplified by extracellular matrix fibers, cells and macroscopic shapes. The main focus of this review is the application of a biomimetic approach by the combination of architectural cues, obtained through the application of micro- and nanofabrication techniques, with the ability to sequester and release growth factors and other bioactive agents in a spatiotemporal controlled manner for bone and Cartilage Engineering.
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novel genipin cross linked chitosan silk fibroin sponges for Cartilage Engineering strategies
Biomacromolecules, 2008Co-Authors: Simone Santos Silva, Manuela E Gomes, Rui L. Reis, João F. Mano, Antonella Motta, Marcia T Rodrigues, Ana F M Pinheiro, Claudio MigliaresiAbstract:The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural Cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from α-helix/random-coil to β-sheet structure, distinct surface morphologies, and pH/swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges’ potential in Cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cell...
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Novel Genipin-Cross-Linked Chitosan/Silk Fibroin Sponges for Cartilage Engineering Strategies
Biomacromolecules, 2008Co-Authors: Simone Santos Silva, Manuela E Gomes, Rui L. Reis, João F. Mano, Antonella Motta, Marcia T Rodrigues, Ana F M Pinheiro, Claudio MigliaresiAbstract:The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural Cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from α-helix/random-coil to β-sheet structure, distinct surface morphologies, and pH/swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges’ potential in Cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cell...
Philippe Galera - One of the best experts on this subject based on the ideXlab platform.
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Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for Cartilage regeneration
Scientific Reports, 2018Co-Authors: Rodolphe Rakic, Magali Demoor, Stéphane Maddens, Nathalie Saulnier, B Bourdon, Philippe GaleraAbstract:Umbilical cord blood mesenchymal stromal/stem cells (UCB-MSCs) and umbilical cord matrix MSCs (UCM-MSCs) have chondrogenic potential and are alternative sources to standard surgically derived bone marrow or adipose tissue collection for Cartilage Engineering. However, the majority of comparative studies explore neonatal MSCs potential only on ISCT benchmark assays accounting for some bias in the reproducibility between in vitro and in clinical studies. Therefore, we characterized equine UCB-MSCs and UCM-MSCs and investigated with particular attention their chondrogenesis potential in 3D culture with BMP-2 + TGF-ß1 in normoxia or hypoxia. We carried out an exhaustive characterization of the extracellular matrix generated by both these two types of MSCs after the induction of chondrogenesis through evaluation of hyaline Cartilage, hypertrophic and osteogenic markers (mRNA, protein and histology levels). Some differences in hypoxia sensitivity and chondrogenesis were observed. UCB-MSCs differentiated into chondrocytes express an abundant, dense and a hyaline-like Cartilage matrix. By contrast, despite their expression of Cartilage markers, UCM-MSCs failed to express a relevant Cartilage matrix after chondrogenic induction. Both MSCs types also displayed intrinsic differences at their undifferentiated basal status, UCB-MSCs expressing higher levels of chondrogenic markers whereas UCM-MSCs synthesizing higher amounts of osteogenic markers. Our results suggest that UCB-MSCs should be preferred for ex-vivo horse Cartilage Engineering. How those results should be translated to in vivo direct Cartilage regeneration remains to be determined through dedicated study.
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Hypoxia Is a Critical Parameter for Chondrogenic Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells in Type I/III Collagen Sponges.
International Journal of Molecular Sciences, 2017Co-Authors: Tangni Gomez-leduc, Philippe Galera, Mélanie Desancé, Florence Legendre, Magalie Hervieu, David Ollitrault, Claire De Vienne, Michel Herlicoviez, Magali DemoorAbstract:Umbilical cord blood (UCB) is an attractive alternative to bone marrow for isolation of mesenchymal stem cells (MSCs) to treat articular Cartilage defects. Here, we set out to determine the growth factors (bone morphogenetic protein 2 (BMP-2) and transforming growth factor-β (TGF-β1)) and oxygen tension effects during chondrogenesis of human UCB-MSCs for Cartilage Engineering. Chondrogenic differentiation was induced using 3D cultures in type I/III collagen sponges with chondrogenic factors in normoxia (21% O2) or hypoxia (
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Molecular mechanism of hypoxia-induced chondrogenesis and its application in in vivo Cartilage tissue Engineering.
Biomaterials, 2012Co-Authors: Elise Duval, Rina Andriamanalijaona, Hervé Bénateau, Soizic Dutoit, Catherine Baugé, Sylvain Leclercq, Philippe Galera, Laurent Poulain, Karim BoumedieneAbstract:Cartilage Engineering is one of the most challenging issue in regenerative medicine, due to its limited self-ability to repair. Here, we assessed Engineering of Cartilage tissue starting from human bone marrow (hBM) stem cells under hypoxic environment and delineated the mechanism whereby chondrogenesis could be conducted without addition of exogenous growth factors. hBM stem cells were cultured in alginate beads and chondrogenesis was monitored by chondrocyte phenotypic markers. Activities and roles of Sox and HIF-1α transcription factors were investigated with complementary approaches of gain and loss of function and provided evidences that HIF-1α is essential for hypoxic induction of chondrogenesis. Thereafter, hBM cells and human articular chondrocytes (HAC) underwent chondrogenesis by 3D and hypoxic culture for 7 days or by ectopic expression of HIF-1α. After subcutaneous implantation of 3 weeks into athymic mice, tissue analysis showed that hypoxia or HIF-1α overexpression is effective and sufficient to induce chondrocyte phenotype in hBM cells, without use of exogenous growth factors. Therefore, this study brings interesting data for a simple and affordable system in biotechnology of Cartilage Engineering.
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Molecular mechanism of hypoxia-induced chondrogenesis and its application in in vivo Cartilage tissue Engineering
Biomaterials, 2012Co-Authors: Elise Duval, Rina Andriamanalijaona, Hervé Bénateau, Soizic Dutoit, Catherine Baugé, Sylvain Leclercq, Philippe Galera, Laurent Poulain, Karim BoumedieneAbstract:Cartilage Engineering is one of the most challenging issue in regenerative medicine, due to its limited self-ability to repair. Here, we assessed Engineering of Cartilage tissue starting from human bone marrow (hBM) stem cells under hypoxic environment and delineated the mechanism whereby chondrogenesis could be conducted without addition of exogenous growth factors. hBM stem cells were cultured in alginate beads and chondrogenesis was monitored by chondrocyte phenotypic markers. Activities and roles of Sox and HIF-1α transcription factors were investigated with complementary approaches of gain and loss of function and provided evidences that HIF-1α is essential for hypoxic induction of chondrogenesis. Thereafter, hBM cells and human articular chondrocytes (HAC) underwent chondrogenesis by 3D and hypoxic culture for 7 days or by ectopic expression of HIF-1α. After subcutaneous implantation of 3 weeks into athymic mice, tissue analysis showed that hypoxia or HIF-1α overexpression is effective and sufficient to induce chondrocyte phenotype in hBM cells, without use of exogenous growth factors. Therefore, this study brings interesting data for a simple and affordable system in biotechnology of Cartilage Engineering. © 2012 Elsevier Ltd.
Daniele Noel - One of the best experts on this subject based on the ideXlab platform.
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The role of pharmacologically active microcarriers releasing TGF-β3 in Cartilage formation in vivo by mesenchymal stem cells
Biomaterials, 2010Co-Authors: Carine Bouffi, Claire Bony, C. Jorgensen, Olivier Thomas, Alexandra Giteau, Marie-claire Venier-julienne, Claudia N. Montero-menei, Daniele NoelAbstract:Cartilage Engineering using mesenchymal stem cells (MSC) will require the use of a scaffold which will act as a support for cell adhesion keeping the cells in the Cartilage defect. Optimally, a tissue engineered construct should allow sustained delivery of bioactive factors capable of inducing MSC differentiation into chondrocytes and should be easily injected inside the Cartilage lesions to avoid surgical operations. We therefore developed pharmacologically active microcarriers (PAM) made of poly-lactic-co-glycolic acid (PLGA) produced using an oil-in-water (o/w) emulsion method. The microspheres were coated with a biomimetic surface of fibronectin (FN) and engineered to release TGF-β3 as a chondrogenic differentiation factor. When human MSCs were incubated in vitro with TGF-β3 releasing FN-coated PAMs in chondrogenic medium, they firmly adhered onto the surface of PAMs rapidly forming cell aggregates. After 3 weeks, strong up-regulation of Cartilage-specific markers was observed both at the mRNA and protein level whereas osteogenic or adipogenic genes could not be detected. Importantly, implantation of MSC/TGF-β3 releasing PAM complexes in SCID mice resulted in the formation of histologically resembling Cartilage which stained positive for chondrocyte markers, collagen II and aggrecan. The present study demonstrated that functionalized PLGA-based microparticles can provide an appropriate environment for chondrogenic differentiation of MSCs and should contribute to injectable biomedical device development improving in vivo Cartilage Engineering.
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Cartilage tissue Engineering towards a biomaterial assisted mesenchymal stem cell therapy
Current Stem Cell Research & Therapy, 2009Co-Authors: Claire Vinatier, C. Jorgensen, Jérôme Guicheux, Pierre Weiss, Christophe Merceron, Carine Bouffi, Jan O Gordeladze, Jeanmarc Brondello, Daniele NoelAbstract:Injuries to articular Cartilage are one of the most challenging issues of musculoskeletal medicine due to the poor intrinsic ability of this tissue for repair. Despite progress in orthopaedic surgery, the lack of efficient modalities of treatment for large chondral defects has prompted research on tissue Engineering combining chondrogenic cells, scaffold materials and environmental factors. The aim of this review is to focus on the recent advances made in exploiting the potentials of cell therapy for Cartilage Engineering. These include: 1) defining the best cell candidates between chondrocytes or multipotent progenitor cells, such as multipotent mesenchymal stromal cells (MSC), in terms of readily available sources for isolation, expansion and repair potential; 2) Engineering biocompatible and biodegradable natural or artificial matrix scaffolds as cell carriers, chondrogenic factors releasing factories and supports for defect filling, 3) identifying more specific growth factors and the appropriate scheme of application that will promote both chondrogenic differentiation and then maintain the differentiated phenotype overtime and 4) evaluating the optimal combinations that will answer to the functional demand placed upon Cartilage tissue replacement in animal models and in clinics. Finally, some of the major obstacles generally encountered in Cartilage Engineering are discussed as well as future trends to overcome these limiting issues for clinical applications.
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Cartilage Engineering a crucial combination of cells biomaterials and biofactors
Trends in Biotechnology, 2009Co-Authors: Claire Vinatier, D. Mrugala, C. Jorgensen, Daniele Noel, Jérôme GuicheuxAbstract:Injuries to articular Cartilage are one of the most challenging issues of musculoskeletal medicine due to the poor intrinsic ability of this tissue for repair. The lack of efficient modalities of treatment has prompted research into tissue Engineering combining chondrogenic cells, scaffold materials and environmental factors. The aim of this review is to focus on the recent advances made in exploiting the potential of biomaterial-assisted cell therapy for Cartilage Engineering. We discuss the requirements for identifying additional specific growth factors and evaluating the optimal combination of cells, growth factors and scaffolds that is able to respond to the functional demand placed upon Cartilage tissue replacement in clinics. Finally, some of the major obstacles encountered in Cartilage Engineering are discussed, as well as future trends in clinical applications.
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Cartilage Engineering: a crucial combination of cells, biomaterials and biofactors
Trends in Biotechnology, 2009Co-Authors: Claire Vinatier, D. Mrugala, C. Jorgensen, Jérôme Guicheux, Daniele NoelAbstract:Injuries to articular Cartilage are one of the most challenging issues of musculoskeletal medicine due to the poor intrinsic ability of this tissue for repair. The lack of efficient modalities of treatment has prompted research into tissue Engineering combining chondrogenic cells, scaffold materials and environmental factors. The aim of this review is to focus on the recent advances made in exploiting the potential of biomaterial-assisted cell therapy for Cartilage Engineering. We discuss the requirements for identifying additional specific growth factors and evaluating the optimal combination of cells, growth factors and scaffolds that is able to respond to the functional demand placed upon Cartilage tissue replacement in clinics. Finally, some of the major obstacles encountered in Cartilage Engineering are discussed, as well as future trends in clinical applications. © 2009 Elsevier Ltd. All rights reserved.
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Multipotent mesenchymal stromal cells in articular diseases
Best Practice & Research: Clinical Rheumatology, 2008Co-Authors: C. Jorgensen, D. Mrugala, Carine Bouffi, Farida Djouad, Daniele NoelAbstract:Although Cartilage defects are common features of osteoarthritis and rheumatoid arthritis, current treatments can rarely restore the full function of native Cartilage. Recent studies have provided new perspectives for Cartilage Engineering using multipotent mesenchymal stromal cells (MSC). Moreover, MSC have been used as immunosuppressant agents in autoimmune diseases and have tested successfully in animal models of arthritis. However, the sequential events occurring during chondrogenesis must be fully understood before we can reproduce the complex molecular events that lead to MSC differentiation and long-term maintenance of Cartilage characteristics in the context of chronic joint inflammation. This chapter focuses on the potential of MSC to repair Cartilage, with an emphasis on the factors that are known to be required in inducing chondrogenesis and on their immunosuppressive potential.