The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
Ayman Al-hendy - One of the best experts on this subject based on the ideXlab platform.
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Towards non-surgical therapy for uterine fibroids: Catechol-O-Methyl Transferase inhibitor shrinks uterine fibroid lesions in the Eker rat model
Human reproduction (Oxford England), 2011Co-Authors: Memy H. Hassan, Hala Fouad, S. Bahashwan, Ayman Al-hendyAbstract:BACKGROUND Uterine leiomyomas (fibroids) are the most common pelvic tumors in women. We assessed the potential therapeutic utility of Ro 41-0960, a synthetic Catechol-O-Methyl Transferase inhibitor (COMTI), in the Eker rat.
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Progesterone-Mediated Regulation of Catechol-O-Methyl Transferase Expression in Endometrial Cancer Cells
Reproductive sciences (Thousand Oaks Calif.), 2007Co-Authors: Sana M. Salih, Salama A. Salama, Amin A. Fadl, Manubai Nagamani, Mohammad Jamaluddin, Leen J. Blok, Curt W. Burger, Ayman Al-hendyAbstract:The effects of estrogen and progesterone on the expression of estrogen-metabolizing enzymes such as Catechol-O-Methyl Transferase (COMT) are not known. COMT converts genotoxic catecholestrogens to anticarcinogenic methoxyestrogens in the endometrium. The aim of this study is to investigate the effect of progesterone on COMT expression in well-differentiated endometrial cancer cells. The wild-type Ishikawa cell line as well as progesterone receptor A- or progesterone receptor B-transfected Ishikawa cells were used for in vitro studies. The regulation of COMT expression by progesterone was studied using Western blots, Hoechst dye DNA proliferation studies, and wild-type and/or site-directed mutagenesis of COMT promoter 1-luciferase reporter gene. Progesterone upregulated COMT protein expression in Ishikawa cells through progesterone receptor A isoform. COMT promoter activity was differentially regulated by the 3 half-site progesterone response elements in the COMT promoter. High doses of 2-ME2 inhibited Ishikawa cell proliferation. These data suggest that COMT expression is hormonally regulated in well-differentiated human endometrial cancer cells. COMT regulation and 2-ME2 production in the endometrium may affect endometrial carcinogenesis.
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Expression and cyclic variations of Catechol-O-Methyl Transferase in human endometrial stroma.
Fertility and sterility, 2007Co-Authors: Sana M. Salih, Salama A. Salama, Amin A. Fadl, Manubai Nagamani, Ayman Al-hendyAbstract:Objective To investigate the role of Catechol-O-Methyl Transferase (COMT) in the regulation of estrogen metabolism in human endometrium. Design Laboratory study. Setting Academic research laboratory. Intervention(s) Immunohistochemistry was used to localize COMT protein in human endometrial tissues. Catechol-O-Methyl Transferase promoter–luciferace reporter gene transactivation assay was used to assess COMT promoter activity in response to estrogen and progesterone treatment in primary human endometrial stroma (pHES) cells. Catechol-O-Methyl Transferase protein and mRNA expression were determined by Western blot and/or real-time polymerase chain reaction. The effect of 2-methoxy estrogen treatment on DNA proliferation, B-cell lymphoma 2, and vascular epithelial growth factor protein expression were assessed by Hoechst and Western blot analyses, respectively. Main Outcome Measure(s) Catechol-O-Methyl Transferase protein and mRNA subcellular localization and expression in human endometrial tissues and pHES cells. Result(s) Catechol-O-Methyl Transferase protein expression in human endometrial tissues was up-regulated in the proliferative phase and down-regulated in the midsecretory phase of the menstrual cycle. Estrogen induced a dose-dependent increase in COMT proximal promotor–luciferace transactivation in pHES cells whereas progesterone inhibited it. Estrogen up-regulated soluble COMT protein isoform expression whereas the addition of progesterone down-regulated it in pHES cells. High doses of 2-methoxy estrogen inhibited endometrial stroma cell proliferation, and down-regulated B-cell lymphoma 2 and vascular epithelial growth factor protein expression. Conclusion(s) Catechol-O-Methyl Transferase expression is hormonally regulated in human endometrial stroma. Catechol-O-Methyl Transferase product, 2-methoxy estrogen, inhibited endometrial stroma cell proliferation and decreased vascular epithelial growth factor and B-cell lymphoma 2 protein expression.
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Hormonal regulation of Catechol-O-Methyl Transferase activity in women with uterine leiomyomas
Fertility and sterility, 2006Co-Authors: Salama A. Salama, Hui Qun Wang, Jukka Tenhunen, Carola Tilgmann, Ayman Al-hendyAbstract:Catechol-O-Methyl Transferase (COMT) expression is higher in leiomyomas compared with paired normal myometrium. The expression of COMT in leiomyoma cells is hormonally regulated-estrogen down-regulates, whereas P and dexamethasone up-regulate, COMT expression.
Inna Belfer - One of the best experts on this subject based on the ideXlab platform.
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Chronic pain after lower abdominal surgery: do Catechol-O-Methyl Transferase/opioid receptor μ-1 polymorphisms contribute?
Molecular pain, 2013Co-Authors: Yuri Kolesnikov, Boris Gabovits, Ariel Levin, Andres Veske, Li Qin, Feng Dai, Inna BelferAbstract:Background Preoperative pain, type of operation and anesthesia, severity of acute postoperative pain, and psychosocial factors have been identified as risk factors for chronic postsurgical pain (CPP). Recently, it has been suggested that genetic factors also contribute to CPP. In this study, we aimed to determine whether the Catechol-O-Methyl Transferase (COMT) and opioid receptor μ-1 (OPRM1) common functional polymorphisms rs4680 and rs1799971 were associated with the incidence, intensity, or duration of CPP in patients after lower abdominal surgery.
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Chronic Pain after Lower Abdominal Surgery: Do Catechol-O-Methyl Transferase/Opioid Receptor μ−1 Polymorphisms Contribute?:
Molecular pain, 2013Co-Authors: Yuri Kolesnikov, Boris Gabovits, Ariel Levin, Andres Veske, Li Qin, Feng Dai, Inna BelferAbstract:Background Preoperative pain, type of operation and anesthesia, severity of acute postoperative pain, and psychosocial factors have been identified as risk factors for chronic postsurgical pain (CPP). Recently, it has been suggested that genetic factors also contribute to CPP. In this study, we aimed to determine whether the Catechol-O-Methyl Transferase (COMT) and opioid receptor μ-1 (OPRM1) common functional polymorphisms rs4680 and rs1799971 were associated with the incidence, intensity, or duration of CPP in patients after lower abdominal surgery.
Nancy C Andreasen - One of the best experts on this subject based on the ideXlab platform.
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catechol o methyl Transferase val158met gene polymorphism in schizophrenia working memory frontal lobe mri morphology and frontal cerebral blood flow
Molecular Psychiatry, 2005Co-Authors: Bengchoon Ho, Thomas H Wassink, Daniel S Oleary, Val C Sheffield, Nancy C AndreasenAbstract:The Catechol-O-Methyl Transferase (COMT) gene is considered a leading schizophrenia candidate gene. Although its role in increasing schizophrenia susceptibility has been conflicting, recent studies suggest the valine allele may contribute to poor cognitive function in schizophrenia. V158M COMT genotype was obtained on 159 schizophrenia patients and 84 healthy controls. The effects of COMT genotype on four measures of working memory/executive functions (Wisconsin Card Sorting, digit span backward, Trail Making and N-back tests) and on MRI frontal brain volumes were examined. Genotype distributions were not significantly different between patients and controls. There were no significant genotype or genotype-by-group effects on any working memory/executive function measures. No genotype or genotype-by-diagnosis interaction effects were found with MRI frontal lobe volumes. Randomization analyses using [15O]H2O positron emission tomography (PET) cerebral blood flow data found Val/Val patients had higher frontal lobe activation than Met/Met patients while performing the one-back task. Overall, these findings do not support a major role for COMT in increasing susceptibility for schizophrenia or in mediating frontal lobe function. Age-related changes and phenotypic heterogeneity of schizophrenia may influence the complex relationships between COMT genotype and cognition.
Yuri Kolesnikov - One of the best experts on this subject based on the ideXlab platform.
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Chronic pain after lower abdominal surgery: do Catechol-O-Methyl Transferase/opioid receptor μ-1 polymorphisms contribute?
Molecular pain, 2013Co-Authors: Yuri Kolesnikov, Boris Gabovits, Ariel Levin, Andres Veske, Li Qin, Feng Dai, Inna BelferAbstract:Background Preoperative pain, type of operation and anesthesia, severity of acute postoperative pain, and psychosocial factors have been identified as risk factors for chronic postsurgical pain (CPP). Recently, it has been suggested that genetic factors also contribute to CPP. In this study, we aimed to determine whether the Catechol-O-Methyl Transferase (COMT) and opioid receptor μ-1 (OPRM1) common functional polymorphisms rs4680 and rs1799971 were associated with the incidence, intensity, or duration of CPP in patients after lower abdominal surgery.
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Chronic Pain after Lower Abdominal Surgery: Do Catechol-O-Methyl Transferase/Opioid Receptor μ−1 Polymorphisms Contribute?:
Molecular pain, 2013Co-Authors: Yuri Kolesnikov, Boris Gabovits, Ariel Levin, Andres Veske, Li Qin, Feng Dai, Inna BelferAbstract:Background Preoperative pain, type of operation and anesthesia, severity of acute postoperative pain, and psychosocial factors have been identified as risk factors for chronic postsurgical pain (CPP). Recently, it has been suggested that genetic factors also contribute to CPP. In this study, we aimed to determine whether the Catechol-O-Methyl Transferase (COMT) and opioid receptor μ-1 (OPRM1) common functional polymorphisms rs4680 and rs1799971 were associated with the incidence, intensity, or duration of CPP in patients after lower abdominal surgery.
Feng Dai - One of the best experts on this subject based on the ideXlab platform.
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Chronic pain after lower abdominal surgery: do Catechol-O-Methyl Transferase/opioid receptor μ-1 polymorphisms contribute?
Molecular pain, 2013Co-Authors: Yuri Kolesnikov, Boris Gabovits, Ariel Levin, Andres Veske, Li Qin, Feng Dai, Inna BelferAbstract:Background Preoperative pain, type of operation and anesthesia, severity of acute postoperative pain, and psychosocial factors have been identified as risk factors for chronic postsurgical pain (CPP). Recently, it has been suggested that genetic factors also contribute to CPP. In this study, we aimed to determine whether the Catechol-O-Methyl Transferase (COMT) and opioid receptor μ-1 (OPRM1) common functional polymorphisms rs4680 and rs1799971 were associated with the incidence, intensity, or duration of CPP in patients after lower abdominal surgery.
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Chronic Pain after Lower Abdominal Surgery: Do Catechol-O-Methyl Transferase/Opioid Receptor μ−1 Polymorphisms Contribute?:
Molecular pain, 2013Co-Authors: Yuri Kolesnikov, Boris Gabovits, Ariel Levin, Andres Veske, Li Qin, Feng Dai, Inna BelferAbstract:Background Preoperative pain, type of operation and anesthesia, severity of acute postoperative pain, and psychosocial factors have been identified as risk factors for chronic postsurgical pain (CPP). Recently, it has been suggested that genetic factors also contribute to CPP. In this study, we aimed to determine whether the Catechol-O-Methyl Transferase (COMT) and opioid receptor μ-1 (OPRM1) common functional polymorphisms rs4680 and rs1799971 were associated with the incidence, intensity, or duration of CPP in patients after lower abdominal surgery.