The Experts below are selected from a list of 166380 Experts worldwide ranked by ideXlab platform
Luigi Panza - One of the best experts on this subject based on the ideXlab platform.
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synthesis of a fluorescent sulfatide for the study of CD1 Antigen binding properties a fluorescent sulfatide for the study of CD1 Antigen binding properties
European Journal of Organic Chemistry, 2004Co-Authors: Laura Franchini, Federica Compostella, Alena Donda, Lucia Mori, Diego Colombo, Gennaro De Libero, Pamela Matto, Fiamma Ronchetti, Luigi PanzaAbstract:A fluorescent derivative of sulfatide, labeled with a dansyl probe at position 6 of galactose, was prepared through a glycosylation reaction between a 6-O-functionalized galactose donor and 3-O-benzoyl-azidosphingosine. The sphingoid acceptor was obtained in high yield through exploitation of a highly stereoselective nucleophilic addition of pentadecyne to 2,3-O-cyclohexylidene-D-glyceraldehyde. The fluorescent sulfatide binds to soluble recombinant human CD1a and is a valuable tool for the study of CD1 Antigen binding properties. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Gennaro De Libero - One of the best experts on this subject based on the ideXlab platform.
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bacterial infections promote t cell recognition of self glycolipids
Immunity, 2005Co-Authors: Gennaro De Libero, Anthony P Moran, Hans Jurgen Gober, Emmanuel Rossy, Abdijapar Shamshiev, Olga Chelnokova, Zaima Mazorra, Silvia Vendetti, Alessandra Sacchi, Martina M PrendergastAbstract:Summary Recognition of self is essential for repertoire selection, immune regulation, and autoimmunity and may be a consequence of infection. Self-induced recognition may represent the escape mechanism adopted by pathogens but may also incite autoimmune diseases. Here, we show that bacterial infection may promote activation of T cells reactive to self-glycosphingolipids (self-GSL). CD1 + Antigen-presenting cells (APCs) infected with bacteria ( Escherichia coli , Bacillus subtilis , Staphylococcus aureus , or Mycobacterium bovis -Bacillus Calmette Guerin [BCG]) or treated with the bacterial components lipopolysaccharide, lipoteichoic acid, or Pam 3 CysSerLys 4 (P 3 CSK 4 ) lipopeptide acquire the capacity to stimulate self-GSL-specific T cells to cytokine release. Immediately after infection, APCs increase the endogenous GSL synthesis and stimulate GSL-specific T cells in a CD1- and T cell receptor (TCR)-dependent manner. This stimulation may contribute to inflammatory responses during bacterial infections and may predispose individuals to autoimmune diseases.
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synthesis of a fluorescent sulfatide for the study of CD1 Antigen binding properties a fluorescent sulfatide for the study of CD1 Antigen binding properties
European Journal of Organic Chemistry, 2004Co-Authors: Laura Franchini, Federica Compostella, Alena Donda, Lucia Mori, Diego Colombo, Gennaro De Libero, Pamela Matto, Fiamma Ronchetti, Luigi PanzaAbstract:A fluorescent derivative of sulfatide, labeled with a dansyl probe at position 6 of galactose, was prepared through a glycosylation reaction between a 6-O-functionalized galactose donor and 3-O-benzoyl-azidosphingosine. The sphingoid acceptor was obtained in high yield through exploitation of a highly stereoselective nucleophilic addition of pentadecyne to 2,3-O-cyclohexylidene-D-glyceraldehyde. The fluorescent sulfatide binds to soluble recombinant human CD1a and is a valuable tool for the study of CD1 Antigen binding properties. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Michael B Brenner - One of the best experts on this subject based on the ideXlab platform.
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CD1 Antigen presentation how it works
Nature Reviews Immunology, 2007Co-Authors: Duarte C Barral, Michael B BrennerAbstract:The classic concept of self-non-self discrimination by the immune system focused on the recognition of fragments from proteins presented by classical MHC molecules. However, the discovery of MHC-class-I-like CD1 Antigen-presentation molecules now explains how the immune system also recognizes the abundant and diverse universe of lipid-containing Antigens. The CD1 molecules bind and present amphipathic lipid Antigens for recognition by T-cell receptors. Here, we outline the recent advances in our understanding of how the processes of CD1 assembly, trafficking, lipid-Antigen binding and T-cell activation are achieved and the new insights into how lipid Antigens differentially elicit CD1-restricted innate and adaptive T-cell responses.
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CD1 Antigen presentation and t cell function
Annual Review of Immunology, 2004Co-Authors: Manfred Brigl, Michael B BrennerAbstract:▪ Abstract This review summarizes the major features of CD1 genes and proteins, the patterns of intracellular trafficking of CD1 molecules, and how they sample different intracellular compartments for self- and foreign lipids. We describe how lipid Antigens bind to CD1 molecules with their alkyl chains buried in hydrophobic pockets and expose their polar lipid headgroup whose fine structure is recognized by the TCR of CD1-restricted T cells. CD1-restricted T cells carry out effector, helper, and adjuvant-like functions and interact with other cell types including macrophages, dendritic cells, NK cells, T cells, and B cells, thereby contributing to both innate and adaptive immune responses. Insights gained from mice and humans now delineate the extensive range of diseases in which CD1-restricted T cells play important roles and reveal differences in the role of CD1a, CD1b, and CD1c in contrast to CD1d. Invariant TCRα chains, self-lipid reactivity, and rapid effector responses empower a subset of CD1d-restr...
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CD1c mediated t cell recognition of isoprenoid glycolipids in mycobacterium tuberculosis infection
Nature, 2000Co-Authors: Branch D Moody, Michael B Brenner, Timo Ulrichs, Walter Muhlecker, David C Young, Sudagar S Gurcha, Ethan P Grant, Jean Pierre Rosat, Catherine E CostelloAbstract:The discovery of the CD1 Antigen presentation pathway has expanded the spectrum of T-cell Antigens to include lipids1,2,3,4, but the range of natural lipid Antigens and functions of CD1-restricted T cells in vivo remain poorly understood. Here we show that the T-cell Antigen receptor and the CD1c protein mediate recognition of an evolutionarily conserved family of isoprenoid glycolipids whose members include essential components of protein glycosylation and cell-wall synthesis pathways. A CD1c-restricted, mycobacteria-specific T-cell line recognized two previously unknown mycobacterial hexosyl-1-phosphoisoprenoids and structurally related mannosyl-β1-phosphodolichols. Responses to mannosyl-β1-phosphodolichols were common among CD1c-restricted T-cell lines and peripheral blood T lymphocytes of human subjects recently infected with M. tuberculosis, but were not seen in naive control subjects. These results define a new class of broadly distributed lipid Antigens presented by the CD1 system during infection in vivo and suggest an immune mechanism for recognition of senescent or transformed cells that are known to have altered dolichol lipids.
Branch D Moody - One of the best experts on this subject based on the ideXlab platform.
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mycobacterium tuberculosis regulates CD1 Antigen presentation pathways through tlr 2
Journal of Immunology, 2005Co-Authors: Carme Rouramir, Isamu Matsunaga, Christopher C Dascher, Matthew J Fenton, Tanyun Cheng, Lisheng Wang, Stanford L Peng, Carsten J Kirschning, Branch D MoodyAbstract:Mycobacterium tuberculosis remains a major pathogen of worldwide importance, which releases lipid Ags that are presented to human T cells during the course of tuberculosis infections. Here we report that cellular infection with live M. tuberculosis or exposure to mycobacterial cell wall products converted CD1− myeloid precursors into competent APCs that expressed group 1 CD1 proteins (CD1a, CD1b, and CD1c). The appearance of group 1 CD1 proteins at the surface of infected or activated cells occurred via transcriptional regulation, and new CD1 protein synthesis and was accompanied by down-regulation of CD1d transcripts and protein. Isolation of CD1-inducing factors from M. tuberculosis using normal phase chromatography, as well as the use of purified natural and synthetic compounds, showed that this process involved polar lipids that signaled through TLR-2, and we found that TLR-2 was necessary for the up-regulation of CD1 protein expression. Thus, mycobacterial cell wall lipids provide two distinct signals for the activation of lipid-reactive T cells: lipid Ags that activate T cell receptors and lipid adjuvants that activate APCs through TLR-2. These dual activation signals may represent a system for selectively promoting the presentation of exogenous foreign lipids by those myeloid APCs, which come into direct contact with pathogens.
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CD1c mediated t cell recognition of isoprenoid glycolipids in mycobacterium tuberculosis infection
Nature, 2000Co-Authors: Branch D Moody, Michael B Brenner, Timo Ulrichs, Walter Muhlecker, David C Young, Sudagar S Gurcha, Ethan P Grant, Jean Pierre Rosat, Catherine E CostelloAbstract:The discovery of the CD1 Antigen presentation pathway has expanded the spectrum of T-cell Antigens to include lipids1,2,3,4, but the range of natural lipid Antigens and functions of CD1-restricted T cells in vivo remain poorly understood. Here we show that the T-cell Antigen receptor and the CD1c protein mediate recognition of an evolutionarily conserved family of isoprenoid glycolipids whose members include essential components of protein glycosylation and cell-wall synthesis pathways. A CD1c-restricted, mycobacteria-specific T-cell line recognized two previously unknown mycobacterial hexosyl-1-phosphoisoprenoids and structurally related mannosyl-β1-phosphodolichols. Responses to mannosyl-β1-phosphodolichols were common among CD1c-restricted T-cell lines and peripheral blood T lymphocytes of human subjects recently infected with M. tuberculosis, but were not seen in naive control subjects. These results define a new class of broadly distributed lipid Antigens presented by the CD1 system during infection in vivo and suggest an immune mechanism for recognition of senescent or transformed cells that are known to have altered dolichol lipids.
Laura Franchini - One of the best experts on this subject based on the ideXlab platform.
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synthesis of a fluorescent sulfatide for the study of CD1 Antigen binding properties a fluorescent sulfatide for the study of CD1 Antigen binding properties
European Journal of Organic Chemistry, 2004Co-Authors: Laura Franchini, Federica Compostella, Alena Donda, Lucia Mori, Diego Colombo, Gennaro De Libero, Pamela Matto, Fiamma Ronchetti, Luigi PanzaAbstract:A fluorescent derivative of sulfatide, labeled with a dansyl probe at position 6 of galactose, was prepared through a glycosylation reaction between a 6-O-functionalized galactose donor and 3-O-benzoyl-azidosphingosine. The sphingoid acceptor was obtained in high yield through exploitation of a highly stereoselective nucleophilic addition of pentadecyne to 2,3-O-cyclohexylidene-D-glyceraldehyde. The fluorescent sulfatide binds to soluble recombinant human CD1a and is a valuable tool for the study of CD1 Antigen binding properties. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)