The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform

Erwin Tschachler - One of the best experts on this subject based on the ideXlab platform.

  • keratinocytes express the CD146 muc18 s endo Antigen in tissue culture and during inflammatory skin diseases
    Journal of Investigative Dermatology, 2000
    Co-Authors: Wolfgang Weninger, Michael Rendl, Michael Mildner, Christoph Mayer, Jozef Ban, Alexandra Geusau, Gottfried Bayer, Adrian Tanew, O Majdic, Erwin Tschachler
    Abstract:

    The CD146 (or MUC18/MEL-CAM) Antigen is a cell adhesion molecule of the immunoglobulin superfamily. Besides in melanoma, expression of CD146 Antigen has been demonstrated in breast epithelia and hair follicles. We studied its expression by human keratinocytes in culture as well as in neoplastic and inflammatory skin diseases. Staining of primary cultured keratinocytes revealed expression of CD146 on the cell membrane, preferentially on cell–cell contact sites. Western blot analysis of keratinocytes detected a band of ≈113 kDa, corresponding to the CD146 protein. In contrast to primary keratinocytes, neither CD146 protein nor mRNA expression was found in the keratinocyte-derived cell lines A431 and HaCaT. Treatment of keratinocytes with the proinflammatory cytokines interleukin-1 and interleukin-6, tumor necrosis factor-α, and interferon-γ, resulted in no change of CD146 expression and incubation with phorbol 12-myristate 13-acetate led to a reduction of CD146 on keratinocytes. By contrast, when culturing keratinocytes in medium devoid of growth supplements, a distinct upregulation was observed as compared with culture in fully supplemented medium. In normal human epidermis expression of the CD146 Antigen was not detectable. It was strongly upregulated, however, on suprabasal keratinocytes in psoriasis, in lichen planus, in the epidermis overlying skin neoplasms, and in viral warts. In squamous cell carcinomas and basal cell carcinomas only a minority of tumor cells expressed CD146. Our findings suggest that the CD146 Antigen represents an activation marker of keratinocytes and may be involved in cutaneous inflammatory tissue reaction.

Wolfgang Weninger - One of the best experts on this subject based on the ideXlab platform.

  • keratinocytes express the CD146 muc18 s endo Antigen in tissue culture and during inflammatory skin diseases
    Journal of Investigative Dermatology, 2000
    Co-Authors: Wolfgang Weninger, Michael Rendl, Michael Mildner, Christoph Mayer, Jozef Ban, Alexandra Geusau, Gottfried Bayer, Adrian Tanew, O Majdic, Erwin Tschachler
    Abstract:

    The CD146 (or MUC18/MEL-CAM) Antigen is a cell adhesion molecule of the immunoglobulin superfamily. Besides in melanoma, expression of CD146 Antigen has been demonstrated in breast epithelia and hair follicles. We studied its expression by human keratinocytes in culture as well as in neoplastic and inflammatory skin diseases. Staining of primary cultured keratinocytes revealed expression of CD146 on the cell membrane, preferentially on cell–cell contact sites. Western blot analysis of keratinocytes detected a band of ≈113 kDa, corresponding to the CD146 protein. In contrast to primary keratinocytes, neither CD146 protein nor mRNA expression was found in the keratinocyte-derived cell lines A431 and HaCaT. Treatment of keratinocytes with the proinflammatory cytokines interleukin-1 and interleukin-6, tumor necrosis factor-α, and interferon-γ, resulted in no change of CD146 expression and incubation with phorbol 12-myristate 13-acetate led to a reduction of CD146 on keratinocytes. By contrast, when culturing keratinocytes in medium devoid of growth supplements, a distinct upregulation was observed as compared with culture in fully supplemented medium. In normal human epidermis expression of the CD146 Antigen was not detectable. It was strongly upregulated, however, on suprabasal keratinocytes in psoriasis, in lichen planus, in the epidermis overlying skin neoplasms, and in viral warts. In squamous cell carcinomas and basal cell carcinomas only a minority of tumor cells expressed CD146. Our findings suggest that the CD146 Antigen represents an activation marker of keratinocytes and may be involved in cutaneous inflammatory tissue reaction.

Michael Rendl - One of the best experts on this subject based on the ideXlab platform.

  • keratinocytes express the CD146 muc18 s endo Antigen in tissue culture and during inflammatory skin diseases
    Journal of Investigative Dermatology, 2000
    Co-Authors: Wolfgang Weninger, Michael Rendl, Michael Mildner, Christoph Mayer, Jozef Ban, Alexandra Geusau, Gottfried Bayer, Adrian Tanew, O Majdic, Erwin Tschachler
    Abstract:

    The CD146 (or MUC18/MEL-CAM) Antigen is a cell adhesion molecule of the immunoglobulin superfamily. Besides in melanoma, expression of CD146 Antigen has been demonstrated in breast epithelia and hair follicles. We studied its expression by human keratinocytes in culture as well as in neoplastic and inflammatory skin diseases. Staining of primary cultured keratinocytes revealed expression of CD146 on the cell membrane, preferentially on cell–cell contact sites. Western blot analysis of keratinocytes detected a band of ≈113 kDa, corresponding to the CD146 protein. In contrast to primary keratinocytes, neither CD146 protein nor mRNA expression was found in the keratinocyte-derived cell lines A431 and HaCaT. Treatment of keratinocytes with the proinflammatory cytokines interleukin-1 and interleukin-6, tumor necrosis factor-α, and interferon-γ, resulted in no change of CD146 expression and incubation with phorbol 12-myristate 13-acetate led to a reduction of CD146 on keratinocytes. By contrast, when culturing keratinocytes in medium devoid of growth supplements, a distinct upregulation was observed as compared with culture in fully supplemented medium. In normal human epidermis expression of the CD146 Antigen was not detectable. It was strongly upregulated, however, on suprabasal keratinocytes in psoriasis, in lichen planus, in the epidermis overlying skin neoplasms, and in viral warts. In squamous cell carcinomas and basal cell carcinomas only a minority of tumor cells expressed CD146. Our findings suggest that the CD146 Antigen represents an activation marker of keratinocytes and may be involved in cutaneous inflammatory tissue reaction.

Michael Mildner - One of the best experts on this subject based on the ideXlab platform.

  • keratinocytes express the CD146 muc18 s endo Antigen in tissue culture and during inflammatory skin diseases
    Journal of Investigative Dermatology, 2000
    Co-Authors: Wolfgang Weninger, Michael Rendl, Michael Mildner, Christoph Mayer, Jozef Ban, Alexandra Geusau, Gottfried Bayer, Adrian Tanew, O Majdic, Erwin Tschachler
    Abstract:

    The CD146 (or MUC18/MEL-CAM) Antigen is a cell adhesion molecule of the immunoglobulin superfamily. Besides in melanoma, expression of CD146 Antigen has been demonstrated in breast epithelia and hair follicles. We studied its expression by human keratinocytes in culture as well as in neoplastic and inflammatory skin diseases. Staining of primary cultured keratinocytes revealed expression of CD146 on the cell membrane, preferentially on cell–cell contact sites. Western blot analysis of keratinocytes detected a band of ≈113 kDa, corresponding to the CD146 protein. In contrast to primary keratinocytes, neither CD146 protein nor mRNA expression was found in the keratinocyte-derived cell lines A431 and HaCaT. Treatment of keratinocytes with the proinflammatory cytokines interleukin-1 and interleukin-6, tumor necrosis factor-α, and interferon-γ, resulted in no change of CD146 expression and incubation with phorbol 12-myristate 13-acetate led to a reduction of CD146 on keratinocytes. By contrast, when culturing keratinocytes in medium devoid of growth supplements, a distinct upregulation was observed as compared with culture in fully supplemented medium. In normal human epidermis expression of the CD146 Antigen was not detectable. It was strongly upregulated, however, on suprabasal keratinocytes in psoriasis, in lichen planus, in the epidermis overlying skin neoplasms, and in viral warts. In squamous cell carcinomas and basal cell carcinomas only a minority of tumor cells expressed CD146. Our findings suggest that the CD146 Antigen represents an activation marker of keratinocytes and may be involved in cutaneous inflammatory tissue reaction.

Christoph Mayer - One of the best experts on this subject based on the ideXlab platform.

  • keratinocytes express the CD146 muc18 s endo Antigen in tissue culture and during inflammatory skin diseases
    Journal of Investigative Dermatology, 2000
    Co-Authors: Wolfgang Weninger, Michael Rendl, Michael Mildner, Christoph Mayer, Jozef Ban, Alexandra Geusau, Gottfried Bayer, Adrian Tanew, O Majdic, Erwin Tschachler
    Abstract:

    The CD146 (or MUC18/MEL-CAM) Antigen is a cell adhesion molecule of the immunoglobulin superfamily. Besides in melanoma, expression of CD146 Antigen has been demonstrated in breast epithelia and hair follicles. We studied its expression by human keratinocytes in culture as well as in neoplastic and inflammatory skin diseases. Staining of primary cultured keratinocytes revealed expression of CD146 on the cell membrane, preferentially on cell–cell contact sites. Western blot analysis of keratinocytes detected a band of ≈113 kDa, corresponding to the CD146 protein. In contrast to primary keratinocytes, neither CD146 protein nor mRNA expression was found in the keratinocyte-derived cell lines A431 and HaCaT. Treatment of keratinocytes with the proinflammatory cytokines interleukin-1 and interleukin-6, tumor necrosis factor-α, and interferon-γ, resulted in no change of CD146 expression and incubation with phorbol 12-myristate 13-acetate led to a reduction of CD146 on keratinocytes. By contrast, when culturing keratinocytes in medium devoid of growth supplements, a distinct upregulation was observed as compared with culture in fully supplemented medium. In normal human epidermis expression of the CD146 Antigen was not detectable. It was strongly upregulated, however, on suprabasal keratinocytes in psoriasis, in lichen planus, in the epidermis overlying skin neoplasms, and in viral warts. In squamous cell carcinomas and basal cell carcinomas only a minority of tumor cells expressed CD146. Our findings suggest that the CD146 Antigen represents an activation marker of keratinocytes and may be involved in cutaneous inflammatory tissue reaction.