The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
Barbara Pienkowskagrela - One of the best experts on this subject based on the ideXlab platform.
-
cytogenetic and flow cytometry evaluation of richter syndrome reveals myc cdkn2a igh alterations with loss of cd52 cd62l and increase of CD71 Antigen expression as the most frequent recurrent abnormalities
American Journal of Clinical Pathology, 2015Co-Authors: Renata Woroniecka, Grzegorz Rymkiewicz, Beata Grygalewicz, Katarzyna Blachnio, Jolanta Rygier, Malgorzata Jarmuzszymczak, Blazej Ratajczak, Barbara PienkowskagrelaAbstract:Objectives: Richter syndrome (RS) is a transformation of chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL) into high-grade lymphoma. There are only limited data on flow cytometry (FCM) and cytogenetics in RS. Methods: In this study, FCM, classic cytogenetics (CC), and fluorescence in situ hybridization (FISH) were performed in eight RS cases. Results: Most cases of RS were characterized by a loss/decrease of CD52 and CD62L and increased CD71 expression. CC identified complex karyotypes, with losses of 9/9p and 17/17p as the most frequent in four of seven cases. Seven RS cases demonstrated MYC abnormalities. Disruptions of CDKN2A and IGH were identified in five of seven and four of seven RS cases, respectively. Conclusions: Newly diagnosed RS is an oncologic emergency, and a quick diagnostic decision is crucial in clinical practice. Therefore, in patients with CLL/SLL and rapidly enlarging asymmetric lymphadenopathy and/or extranodal tumors, we strongly advise FCM of fine-needle aspiration biopsy (FNAB) material, including CD62L, CD52, and CD71 analysis as well as assessment of karyotype and at least MYC abnormalities by FISH of the same FNAB material. Loss of CD52 expression in RS most likely predicts resistance to alemtuzumab therapy, which is frequently used in CLL.
Renata Woroniecka - One of the best experts on this subject based on the ideXlab platform.
-
cytogenetic and flow cytometry evaluation of richter syndrome reveals myc cdkn2a igh alterations with loss of cd52 cd62l and increase of CD71 Antigen expression as the most frequent recurrent abnormalities
American Journal of Clinical Pathology, 2015Co-Authors: Renata Woroniecka, Grzegorz Rymkiewicz, Beata Grygalewicz, Katarzyna Blachnio, Jolanta Rygier, Malgorzata Jarmuzszymczak, Blazej Ratajczak, Barbara PienkowskagrelaAbstract:Objectives: Richter syndrome (RS) is a transformation of chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL) into high-grade lymphoma. There are only limited data on flow cytometry (FCM) and cytogenetics in RS. Methods: In this study, FCM, classic cytogenetics (CC), and fluorescence in situ hybridization (FISH) were performed in eight RS cases. Results: Most cases of RS were characterized by a loss/decrease of CD52 and CD62L and increased CD71 expression. CC identified complex karyotypes, with losses of 9/9p and 17/17p as the most frequent in four of seven cases. Seven RS cases demonstrated MYC abnormalities. Disruptions of CDKN2A and IGH were identified in five of seven and four of seven RS cases, respectively. Conclusions: Newly diagnosed RS is an oncologic emergency, and a quick diagnostic decision is crucial in clinical practice. Therefore, in patients with CLL/SLL and rapidly enlarging asymmetric lymphadenopathy and/or extranodal tumors, we strongly advise FCM of fine-needle aspiration biopsy (FNAB) material, including CD62L, CD52, and CD71 analysis as well as assessment of karyotype and at least MYC abnormalities by FISH of the same FNAB material. Loss of CD52 expression in RS most likely predicts resistance to alemtuzumab therapy, which is frequently used in CLL.
Lorenzo Bonomo - One of the best experts on this subject based on the ideXlab platform.
-
modulating effects on cd25 and CD71 Antigen expression by lectin stimulated t lymphocytes in the elderly
Immunopharmacology and Immunotoxicology, 1991Co-Authors: S Antonaci, C Tortorella, A Polignano, A Ottolenghi, E Jirillo, Lorenzo BonomoAbstract:AbstractDuring the last few years, several observations outline that the impaired T lymphocyte proliferative capacity in the elderly is due to a reduced interleukin 2 (IL-2) release. to further investigate the activation process during lectin stimulation, aged peripheral blood mononuclear cells (PBMC) were stimulated with phytohemagglutinin (PHA) and assessed for CD25 (IL-2 receptor) and CD71 (transferrin receptor) expression at different intervals of time. Our results provided evidence for a significant decline of both structure induction, above all in the later phase of culture. Indomethacin (INDO) treatment gave rise to an enhancement of CD71 Antigen expression only, while prostaglandin E2 (PGE2) supplementation to culture media further decreased either CD25 or CD71 receptor induction. Interferon (IFN)-α and IFN-γ treatment failed to modulate the frequency of CD25+ and/or CD71+ cells. Finally, the expression of CD71 receptor was increased by deferoxamine supplementation, this suggesting a partial invol...
Grzegorz Rymkiewicz - One of the best experts on this subject based on the ideXlab platform.
-
cytogenetic and flow cytometry evaluation of richter syndrome reveals myc cdkn2a igh alterations with loss of cd52 cd62l and increase of CD71 Antigen expression as the most frequent recurrent abnormalities
American Journal of Clinical Pathology, 2015Co-Authors: Renata Woroniecka, Grzegorz Rymkiewicz, Beata Grygalewicz, Katarzyna Blachnio, Jolanta Rygier, Malgorzata Jarmuzszymczak, Blazej Ratajczak, Barbara PienkowskagrelaAbstract:Objectives: Richter syndrome (RS) is a transformation of chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL) into high-grade lymphoma. There are only limited data on flow cytometry (FCM) and cytogenetics in RS. Methods: In this study, FCM, classic cytogenetics (CC), and fluorescence in situ hybridization (FISH) were performed in eight RS cases. Results: Most cases of RS were characterized by a loss/decrease of CD52 and CD62L and increased CD71 expression. CC identified complex karyotypes, with losses of 9/9p and 17/17p as the most frequent in four of seven cases. Seven RS cases demonstrated MYC abnormalities. Disruptions of CDKN2A and IGH were identified in five of seven and four of seven RS cases, respectively. Conclusions: Newly diagnosed RS is an oncologic emergency, and a quick diagnostic decision is crucial in clinical practice. Therefore, in patients with CLL/SLL and rapidly enlarging asymmetric lymphadenopathy and/or extranodal tumors, we strongly advise FCM of fine-needle aspiration biopsy (FNAB) material, including CD62L, CD52, and CD71 analysis as well as assessment of karyotype and at least MYC abnormalities by FISH of the same FNAB material. Loss of CD52 expression in RS most likely predicts resistance to alemtuzumab therapy, which is frequently used in CLL.
Blazej Ratajczak - One of the best experts on this subject based on the ideXlab platform.
-
cytogenetic and flow cytometry evaluation of richter syndrome reveals myc cdkn2a igh alterations with loss of cd52 cd62l and increase of CD71 Antigen expression as the most frequent recurrent abnormalities
American Journal of Clinical Pathology, 2015Co-Authors: Renata Woroniecka, Grzegorz Rymkiewicz, Beata Grygalewicz, Katarzyna Blachnio, Jolanta Rygier, Malgorzata Jarmuzszymczak, Blazej Ratajczak, Barbara PienkowskagrelaAbstract:Objectives: Richter syndrome (RS) is a transformation of chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL) into high-grade lymphoma. There are only limited data on flow cytometry (FCM) and cytogenetics in RS. Methods: In this study, FCM, classic cytogenetics (CC), and fluorescence in situ hybridization (FISH) were performed in eight RS cases. Results: Most cases of RS were characterized by a loss/decrease of CD52 and CD62L and increased CD71 expression. CC identified complex karyotypes, with losses of 9/9p and 17/17p as the most frequent in four of seven cases. Seven RS cases demonstrated MYC abnormalities. Disruptions of CDKN2A and IGH were identified in five of seven and four of seven RS cases, respectively. Conclusions: Newly diagnosed RS is an oncologic emergency, and a quick diagnostic decision is crucial in clinical practice. Therefore, in patients with CLL/SLL and rapidly enlarging asymmetric lymphadenopathy and/or extranodal tumors, we strongly advise FCM of fine-needle aspiration biopsy (FNAB) material, including CD62L, CD52, and CD71 analysis as well as assessment of karyotype and at least MYC abnormalities by FISH of the same FNAB material. Loss of CD52 expression in RS most likely predicts resistance to alemtuzumab therapy, which is frequently used in CLL.