The Experts below are selected from a list of 771 Experts worldwide ranked by ideXlab platform

Kwai Lin Thong - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of a modified Cefsulodin irgasan novobiocin agar for isolation of yersinia spp
    PLOS ONE, 2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria.

  • Growth of Y. enterocolitica on CIN and modified CIN under different incubation conditions compared with that on LBA (control medium).
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aCIN, Cefsulodin-Irgasan-Novobiocin.bLBA, Luria-Bertani agar.cmCIN, modified CIN.Growth of Y. enterocolitica on CIN and modified CIN under different incubation conditions compared with that on LBA (control medium).

  • Recovery rate of Y. enterocolitica from the 52 naturally contaminated rectal swabs from swine.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    The prevalence of Y. enterocolitica determined by using a duplex PCR targeting the Y. enterocolitica-specific 16S rRNA and ail genes [11] was 2/52 (3.8%).aCIN, Cefsulodin-Irgasan-Novobiocin.bmCIN, modified CIN.cPBS, phosphate buffered saline, a cold enrichment at 4°C for three weeks [8].dKOH, post-enrichment alkaline treatment.Recovery rate of Y. enterocolitica from the 52 naturally contaminated rectal swabs from swine.

  • Colony morphology of Yersinia spp. and other bacteria on CIN and modified CIN.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aCIN, Cefsulodin-Irgasan-Novobiocin.bae, aerobic.cmic, microaerophilic.dNG, no growth.eRbe, red bull's eye.fBc, black centre.gP, pink.hC, colourless.iBp, brown diffusible pigment.IP, Institut Pasteur, strain collection of the French Yersinia Reference laboratory.ATCC, American Type Culture Collection.Others, strain collection of the Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya, Malaysia.Colony morphology of Yersinia spp. and other bacteria on CIN and modified CIN.

  • Recovery of Y. enterocolitica from artificially prepared bacterial mixtures and artificially contaminated raw pork.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aTrue and false positive Y. enterocolitica colonies were determined by using a duplex PCR targeting the Y. enterocolitica-specific 16S rRNA and ail genes [11].bMixture of bacteria exhibiting Yersinia-like colonies on CIN agar (C. freundii, C. braakii, E. cloacae, P. rettgeri, A. hydrophila).cCIN, Cefsulodin-Irgasan-Novobiocin.Recovery of Y. enterocolitica from artificially prepared bacterial mixtures and artificially contaminated raw pork.

Takeshi Nishino - One of the best experts on this subject based on the ideXlab platform.

  • substrate specificities of mexab oprm mexcd oprj and mexxy oprm efflux pumps in pseudomonas aeruginosa
    Antimicrobial Agents and Chemotherapy, 2000
    Co-Authors: Nobuhisa Masuda, Eiko Sakagawa, Satoshi Ohya, Naomasa Gotoh, Hideto Tsujimoto, Takeshi Nishino
    Abstract:

    To find the exact substrate specificities of three species of tripartite efflux systems of Pseudomonas aeruginosa, MexAB-OprM, MexCD-OprJ, and MexXY-OprM, we constructed a series of isogenic mutants, each of which constitutively overproduced one of the three efflux systems and lacked the other two, and their isogenic mutants, which lacked all these systems. Comparison of the susceptibilities of the constructed mutants to 52 antimicrobial agents belonging to various groups suggested the following substrate specificities. All of the efflux systems extrude a wide variety of antimicrobial agent groups, i.e., quinolones, macrolides, tetracyclines, lincomycin, chloramphenicol, most penicillins (all but carbenicillin and sulbenicillin), most cephems (all but Cefsulodin and ceftazidime), meropenem, and S-4661, but none of them extrude polymyxin B or imipenem. Extrusion of aminoglycosides is specific to MexXY-OprM, and extrusion of a group of the beta-lactams, i.e., carbenicillin, sulbenicillin, ceftazidime, moxalactam, and aztreonam, is specific to MexAB-OprM. Moreover, MexAB-OprM and MexCD-OprJ extrude novobiocin, Cefsulodin, and flomoxef, while MexXY-OprM does not. These substrate specificities are distinct from those reported previously.

Lai Kuan Tan - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of a modified Cefsulodin irgasan novobiocin agar for isolation of yersinia spp
    PLOS ONE, 2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria.

  • Growth of Y. enterocolitica on CIN and modified CIN under different incubation conditions compared with that on LBA (control medium).
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aCIN, Cefsulodin-Irgasan-Novobiocin.bLBA, Luria-Bertani agar.cmCIN, modified CIN.Growth of Y. enterocolitica on CIN and modified CIN under different incubation conditions compared with that on LBA (control medium).

  • Recovery rate of Y. enterocolitica from the 52 naturally contaminated rectal swabs from swine.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    The prevalence of Y. enterocolitica determined by using a duplex PCR targeting the Y. enterocolitica-specific 16S rRNA and ail genes [11] was 2/52 (3.8%).aCIN, Cefsulodin-Irgasan-Novobiocin.bmCIN, modified CIN.cPBS, phosphate buffered saline, a cold enrichment at 4°C for three weeks [8].dKOH, post-enrichment alkaline treatment.Recovery rate of Y. enterocolitica from the 52 naturally contaminated rectal swabs from swine.

  • Colony morphology of Yersinia spp. and other bacteria on CIN and modified CIN.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aCIN, Cefsulodin-Irgasan-Novobiocin.bae, aerobic.cmic, microaerophilic.dNG, no growth.eRbe, red bull's eye.fBc, black centre.gP, pink.hC, colourless.iBp, brown diffusible pigment.IP, Institut Pasteur, strain collection of the French Yersinia Reference laboratory.ATCC, American Type Culture Collection.Others, strain collection of the Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya, Malaysia.Colony morphology of Yersinia spp. and other bacteria on CIN and modified CIN.

  • Recovery of Y. enterocolitica from artificially prepared bacterial mixtures and artificially contaminated raw pork.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aTrue and false positive Y. enterocolitica colonies were determined by using a duplex PCR targeting the Y. enterocolitica-specific 16S rRNA and ail genes [11].bMixture of bacteria exhibiting Yersinia-like colonies on CIN agar (C. freundii, C. braakii, E. cloacae, P. rettgeri, A. hydrophila).cCIN, Cefsulodin-Irgasan-Novobiocin.Recovery of Y. enterocolitica from artificially prepared bacterial mixtures and artificially contaminated raw pork.

Nobuhisa Masuda - One of the best experts on this subject based on the ideXlab platform.

  • substrate specificities of mexab oprm mexcd oprj and mexxy oprm efflux pumps in pseudomonas aeruginosa
    Antimicrobial Agents and Chemotherapy, 2000
    Co-Authors: Nobuhisa Masuda, Eiko Sakagawa, Satoshi Ohya, Naomasa Gotoh, Hideto Tsujimoto, Takeshi Nishino
    Abstract:

    To find the exact substrate specificities of three species of tripartite efflux systems of Pseudomonas aeruginosa, MexAB-OprM, MexCD-OprJ, and MexXY-OprM, we constructed a series of isogenic mutants, each of which constitutively overproduced one of the three efflux systems and lacked the other two, and their isogenic mutants, which lacked all these systems. Comparison of the susceptibilities of the constructed mutants to 52 antimicrobial agents belonging to various groups suggested the following substrate specificities. All of the efflux systems extrude a wide variety of antimicrobial agent groups, i.e., quinolones, macrolides, tetracyclines, lincomycin, chloramphenicol, most penicillins (all but carbenicillin and sulbenicillin), most cephems (all but Cefsulodin and ceftazidime), meropenem, and S-4661, but none of them extrude polymyxin B or imipenem. Extrusion of aminoglycosides is specific to MexXY-OprM, and extrusion of a group of the beta-lactams, i.e., carbenicillin, sulbenicillin, ceftazidime, moxalactam, and aztreonam, is specific to MexAB-OprM. Moreover, MexAB-OprM and MexCD-OprJ extrude novobiocin, Cefsulodin, and flomoxef, while MexXY-OprM does not. These substrate specificities are distinct from those reported previously.

Peck Toung Ooi - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of a modified Cefsulodin irgasan novobiocin agar for isolation of yersinia spp
    PLOS ONE, 2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria.

  • Growth of Y. enterocolitica on CIN and modified CIN under different incubation conditions compared with that on LBA (control medium).
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aCIN, Cefsulodin-Irgasan-Novobiocin.bLBA, Luria-Bertani agar.cmCIN, modified CIN.Growth of Y. enterocolitica on CIN and modified CIN under different incubation conditions compared with that on LBA (control medium).

  • Recovery rate of Y. enterocolitica from the 52 naturally contaminated rectal swabs from swine.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    The prevalence of Y. enterocolitica determined by using a duplex PCR targeting the Y. enterocolitica-specific 16S rRNA and ail genes [11] was 2/52 (3.8%).aCIN, Cefsulodin-Irgasan-Novobiocin.bmCIN, modified CIN.cPBS, phosphate buffered saline, a cold enrichment at 4°C for three weeks [8].dKOH, post-enrichment alkaline treatment.Recovery rate of Y. enterocolitica from the 52 naturally contaminated rectal swabs from swine.

  • Colony morphology of Yersinia spp. and other bacteria on CIN and modified CIN.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aCIN, Cefsulodin-Irgasan-Novobiocin.bae, aerobic.cmic, microaerophilic.dNG, no growth.eRbe, red bull's eye.fBc, black centre.gP, pink.hC, colourless.iBp, brown diffusible pigment.IP, Institut Pasteur, strain collection of the French Yersinia Reference laboratory.ATCC, American Type Culture Collection.Others, strain collection of the Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya, Malaysia.Colony morphology of Yersinia spp. and other bacteria on CIN and modified CIN.

  • Recovery of Y. enterocolitica from artificially prepared bacterial mixtures and artificially contaminated raw pork.
    2014
    Co-Authors: Lai Kuan Tan, Peck Toung Ooi, Elisabeth Carniel, Kwai Lin Thong
    Abstract:

    aTrue and false positive Y. enterocolitica colonies were determined by using a duplex PCR targeting the Y. enterocolitica-specific 16S rRNA and ail genes [11].bMixture of bacteria exhibiting Yersinia-like colonies on CIN agar (C. freundii, C. braakii, E. cloacae, P. rettgeri, A. hydrophila).cCIN, Cefsulodin-Irgasan-Novobiocin.Recovery of Y. enterocolitica from artificially prepared bacterial mixtures and artificially contaminated raw pork.