The Experts below are selected from a list of 31323 Experts worldwide ranked by ideXlab platform
Chris Hawes - One of the best experts on this subject based on the ideXlab platform.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
Imogen Sparkes - One of the best experts on this subject based on the ideXlab platform.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
A Kearns - One of the best experts on this subject based on the ideXlab platform.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
John Runions - One of the best experts on this subject based on the ideXlab platform.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
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rapid transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Nature Protocols, 2006Co-Authors: Imogen Sparkes, John Runions, A Kearns, Chris HawesAbstract:Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in Cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal Cells is a relatively fast Technique to assess expression of genes of interest. These Cells can be used to generate stable plant lines using a more time-consuming, Cell Culture Technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.
N J Bundred - One of the best experts on this subject based on the ideXlab platform.
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novel Cell Culture Technique for primary ductal carcinoma in situ role of notch and epidermal growth factor receptor signaling pathways
Journal of the National Cancer Institute, 2007Co-Authors: Gillian Farnie, Robert Clarke, Katherine Spence, Natasha Pinnock, Keith Brennan, Neil G Anderson, N J BundredAbstract:BACKGROUND: The epidermal growth factor receptor (EGFR) and Notch signaling pathways have been implicated in self-renewal of normal breast stem Cells. We investigated the involvement of these signaling pathways in ductal carcinoma in situ (DCIS) of the breast. METHODS: Samples of normal breast tissue (n = 15), pure DCIS tissue of varying grades (n = 35), and DCIS tissue surrounding an invasive cancer (n = 7) were used for nonadherent (i.e., mammosphere) Culture. Mammosphere Cultures were treated at day 0 with gefitinib (an EGFR inhibitor), DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester) (a gamma-secretase inhibitor), or Notch 4-neutralizing antibody. Mammosphere-forming efficiency (MFE) was calculated by dividing the number of mammospheres of 60 microm or more formed by the number of single Cells seeded and is expressed as a percentage. The Notch 1 intraCellular domain (NICD) was detected immunohistochemically in paraffin-embedded DCIS tissue from 50 patients with at least 60 months of follow-up. All statistical tests were two-sided. RESULTS: DCIS had a greater MFE than normal breast tissue (1.5% versus 0.5%, difference = 1%, 95% confidence interval [CI] = 0.62% to 1.25%, P<.001). High-grade DCIS had a greater MFE than low-grade DCIS (1.6% versus 1.09%, difference = 0.51%, 95% CI = 0.07% to 0.94%, P = .01). The MFE of high-grade DCIS treated with gefitinib in the absence of exogenous EGF was lower than that of high-grade DCIS treated with mammosphere medium lacking gefitinib and exogenous EGF (0.56% versus 1.36%, difference 0.8%, 95% CI = 0.33% to 1.4%, P = .004). Increased Notch signaling as detected by NICD staining was associated with recurrence at 5 years (P = .012). DCIS MFE was reduced when Notch signaling was inhibited using either DAPT (0.89% versus 0.51%, difference = 0.38%, 95% CI = 0.2% to 0.6%, P<.001) or a Notch 4-neutralizing antibody (0.97% versus 0.2%, difference = 0.77%, 95% CI = 0.52% to 1.0%, P<.001). CONCLUSION: We describe a novel primary Culture Technique for DCIS. Inhibition of the EGFR or Notch signaling pathways reduced DCIS MFE.