The Experts below are selected from a list of 198048 Experts worldwide ranked by ideXlab platform

Randall W King - One of the best experts on this subject based on the ideXlab platform.

  • the insulin receptor adaptor irs2 is an apc c substrate that promotes Cell Cycle Protein expression and a robust spindle assembly checkpoint
    Molecular & Cellular Proteomics, 2020
    Co-Authors: Sandhya Manohar, Steven P Gygi, Randall W King
    Abstract:

    Insulin receptor substrate 2 (IRS2) is an essential adaptor that mediates signaling downstream of the insulin receptor and other receptor tyrosine kinases. Transduction through IRS2-dependent pathways is important for coordinating metabolic homeostasis, and dysregulation of IRS2 causes systemic insulin signaling defects. Despite the importance of maintaining proper IRS2 abundance, little is known about what factors mediate its Protein stability. We conducted an unbiased proteomic screen to uncover novel substrates of the Anaphase Promoting Complex/Cyclosome (APC/C), a ubiquitin ligase that controls the abundance of key Cell Cycle regulators. We found that IRS2 levels are regulated by APC/C activity and that IRS2 is a direct APC/C target in G1. Consistent with the APC/C's role in degrading Cell Cycle regulators, quantitative proteomic analysis of IRS2-null Cells revealed a deficiency in Proteins involved in Cell Cycle progression. We further show that Cells lacking IRS2 display a weakened spindle assembly checkpoint in Cells treated with microtubule inhibitors. Together, these findings reveal a new pathway for IRS2 turnover and indicate that IRS2 is a component of the Cell Cycle control system in addition to acting as an essential metabolic regulator.

  • the insulin receptor adaptor irs2 is an apc c substrate that promotes Cell Cycle Protein expression and a robust spindle assembly checkpoint
    bioRxiv, 2020
    Co-Authors: Sandhya Manohar, Steven P Gygi, Randall W King
    Abstract:

    Insulin receptor substrate 2 (IRS2) is an essential adaptor that mediates signaling downstream of the insulin receptor and other receptor tyrosine kinases. Transduction through IRS2-dependent pathways is important for coordinating metabolic homeostasis, and dysregulation of IRS2 causes systemic insulin signaling defects. Despite the importance of maintaining proper IRS2 abundance, little is known about what factors mediate its Protein stability. We conducted an unbiased proteomic screen to uncover novel substrates of the Anaphase Promoting Complex/Cyclosome (APC/C), a ubiquitin ligase that controls the abundance of key Cell Cycle regulators. Surprisingly, we found that IRS2 levels are regulated by APC/C activity and that IRS2 is a direct APC/C target in G1. Consistent with the role of APC/C in degrading Cell Cycle regulators, we find that IRS2-null Cells are deficient in Proteins involved in Cell Cycle progression and display spindle assembly checkpoint defects during M-phase. Together, these findings reveal a new pathway for IRS2 turnover and indicate that IRS2 is a critical component of the Cell Cycle control system in addition to acting as an essential metabolic regulator.

Tian Keli - One of the best experts on this subject based on the ideXlab platform.

  • Oridonin nanosuspension was more effective than free oridonin on G(2)/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line
    international journal of nanomedicine, 2012
    Co-Authors: Qi Xiaoli, Zhang Dianrui, Xu Xia, Feng Feifei, Ren Guijie, Chu Qianqian, Zhang Qiang, Tian Keli
    Abstract:

    Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 Cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on Cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and Cell Cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate Cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and Cell Cycle Protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G(2)/M phase Cell Cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax Protein expression was decreased and caspase-3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G(2)/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000302735200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Nanoscience & NanotechnologyPharmacology & PharmacySCI(E)13ARTICLE1793-1804

  • Oridonin nanosuspension was more effective than free oridonin on G2/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line
    Dove Medical Press, 2026
    Co-Authors: Qi Xiaoli, Zhang Dianrui, Xu Xia, Feng Feifei, Ren Guijie, Chu Qianqian, Zhang Qiang, Tian Keli
    Abstract:

    Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 Cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on Cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and Cell Cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate Cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and Cell Cycle Protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G2/M phase Cell Cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax Protein expression was decreased and caspase- 3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G2/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line

Keli Tian - One of the best experts on this subject based on the ideXlab platform.

  • oridonin nanosuspension was more effective than free oridonin on g2 m Cell Cycle arrest and apoptosis in the human pancreatic cancer panc 1 Cell line
    International Journal of Nanomedicine, 2012
    Co-Authors: Dianrui Zhang, Feifei Feng, Guijie Ren, Qianqian Chu, Qiang Zhang, Keli Tian
    Abstract:

    Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 Cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on Cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and Cell Cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate Cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and Cell Cycle Protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G(2)/M phase Cell Cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax Protein expression was decreased and caspase- 3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G(2)/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line.

Sandhya Manohar - One of the best experts on this subject based on the ideXlab platform.

  • the insulin receptor adaptor irs2 is an apc c substrate that promotes Cell Cycle Protein expression and a robust spindle assembly checkpoint
    Molecular & Cellular Proteomics, 2020
    Co-Authors: Sandhya Manohar, Steven P Gygi, Randall W King
    Abstract:

    Insulin receptor substrate 2 (IRS2) is an essential adaptor that mediates signaling downstream of the insulin receptor and other receptor tyrosine kinases. Transduction through IRS2-dependent pathways is important for coordinating metabolic homeostasis, and dysregulation of IRS2 causes systemic insulin signaling defects. Despite the importance of maintaining proper IRS2 abundance, little is known about what factors mediate its Protein stability. We conducted an unbiased proteomic screen to uncover novel substrates of the Anaphase Promoting Complex/Cyclosome (APC/C), a ubiquitin ligase that controls the abundance of key Cell Cycle regulators. We found that IRS2 levels are regulated by APC/C activity and that IRS2 is a direct APC/C target in G1. Consistent with the APC/C's role in degrading Cell Cycle regulators, quantitative proteomic analysis of IRS2-null Cells revealed a deficiency in Proteins involved in Cell Cycle progression. We further show that Cells lacking IRS2 display a weakened spindle assembly checkpoint in Cells treated with microtubule inhibitors. Together, these findings reveal a new pathway for IRS2 turnover and indicate that IRS2 is a component of the Cell Cycle control system in addition to acting as an essential metabolic regulator.

  • the insulin receptor adaptor irs2 is an apc c substrate that promotes Cell Cycle Protein expression and a robust spindle assembly checkpoint
    bioRxiv, 2020
    Co-Authors: Sandhya Manohar, Steven P Gygi, Randall W King
    Abstract:

    Insulin receptor substrate 2 (IRS2) is an essential adaptor that mediates signaling downstream of the insulin receptor and other receptor tyrosine kinases. Transduction through IRS2-dependent pathways is important for coordinating metabolic homeostasis, and dysregulation of IRS2 causes systemic insulin signaling defects. Despite the importance of maintaining proper IRS2 abundance, little is known about what factors mediate its Protein stability. We conducted an unbiased proteomic screen to uncover novel substrates of the Anaphase Promoting Complex/Cyclosome (APC/C), a ubiquitin ligase that controls the abundance of key Cell Cycle regulators. Surprisingly, we found that IRS2 levels are regulated by APC/C activity and that IRS2 is a direct APC/C target in G1. Consistent with the role of APC/C in degrading Cell Cycle regulators, we find that IRS2-null Cells are deficient in Proteins involved in Cell Cycle progression and display spindle assembly checkpoint defects during M-phase. Together, these findings reveal a new pathway for IRS2 turnover and indicate that IRS2 is a critical component of the Cell Cycle control system in addition to acting as an essential metabolic regulator.

Qi Xiaoli - One of the best experts on this subject based on the ideXlab platform.

  • Oridonin nanosuspension was more effective than free oridonin on G(2)/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line
    international journal of nanomedicine, 2012
    Co-Authors: Qi Xiaoli, Zhang Dianrui, Xu Xia, Feng Feifei, Ren Guijie, Chu Qianqian, Zhang Qiang, Tian Keli
    Abstract:

    Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 Cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on Cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and Cell Cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate Cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and Cell Cycle Protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G(2)/M phase Cell Cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax Protein expression was decreased and caspase-3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G(2)/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000302735200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Nanoscience & NanotechnologyPharmacology & PharmacySCI(E)13ARTICLE1793-1804

  • Oridonin nanosuspension was more effective than free oridonin on G2/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line
    Dove Medical Press, 2026
    Co-Authors: Qi Xiaoli, Zhang Dianrui, Xu Xia, Feng Feifei, Ren Guijie, Chu Qianqian, Zhang Qiang, Tian Keli
    Abstract:

    Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 Cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on Cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and Cell Cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate Cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and Cell Cycle Protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G2/M phase Cell Cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax Protein expression was decreased and caspase- 3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G2/M Cell Cycle arrest and apoptosis in the human pancreatic cancer PANC-1 Cell line