The Experts below are selected from a list of 113187 Experts worldwide ranked by ideXlab platform
Eugène Vorobiev - One of the best experts on this subject based on the ideXlab platform.
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Current insights in yeast Cell Disruption technologies for oil recovery: A review
Chemical Engineering and Processing - Process Intensification, 2020Co-Authors: Mohamed Koubaa, Nabila Imatoukene, Lucie Drévillon, Eugène VorobievAbstract:Single Cell oils have been considered as potential replacers of plant and animal oils, and are mainly produced by yeasts, molds, and algae. Oleaginous yeasts have taken special attention due to the similarity of their metabolism with that of higher plants, and the numerous genetic tools available for their modification. However, a special need has been reported for efficient Cell Disruption technology due to the rigidity of their Cell wall compared to other biological ones. This makes the conventional maceration for oil recovery usually incomplete. Several mechanical and non-mechanical Cell Disruption technologies have been reported in the literature and are summarized in this review. A special focus is given for bead milling, high-pressure homogenization, ultrasound-, and microwave-assisted extraction. Their advantages and limitations, as well as the future potential strategies for oil recovery from oleaginous yeasts, are discussed.
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Cell Disruption pre-treatments towards an effective recovery of oil from Yarrowia lipolytica oleaginous yeast
Biomass and Bioenergy, 2019Co-Authors: Lucie Drévillon, Mohamed Koubaa, Jean-marc Nicaud, Eugène VorobievAbstract:The aim of this work was to evaluate the efficiency of different Cell Disruption techniques on oil extraction from Y. lipolytica oleaginous yeast. The techniques tested were mechanical expression (ME), moderate pulsed electric field assisted mechanical expression (MPEF-ME), high pulsed electric fields (HPEF), high voltage electrical discharges (HVED), ultrasound (US), and high-pressure homogenization (HPH). The impact of each Cell Disruption technique on fatty acid composition was investigated. Results show that the most efficient technique for Y. lipolytica Disruption was HPH leading to the obtaining of 83.8 ± 4.8% oil extraction yield, compared to only 19.8 ± 0.5% for the control, without any pre-treatment. In terms of fatty acid composition, HVED induced significant changes in almost all the analyzed fatty acids, while US was the softest technique. HPH, ME, and MPEF-ME induced changes in the content of some fatty acids.
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Yeast Cell Disruption strategies for recovery of intraCellular bio-active compounds — A review
Innovative Food Science & Emerging Technologies, 2016Co-Authors: Dan Liu, Lijun Ding, Jianxia Sun, Nadia Boussetta, Eugène VorobievAbstract:Abstract Yeasts are cheap, attractive and easily available residual sources of valuable bio-active compounds. Extraction of these compounds requires to break the yeast Cells. So efficient damage of Cell wall has become an important issue to be resolved. The aim of this paper is to review the potential of some emerging Cell Disruption techniques for recovery of intraCellular bio-active compounds from Baker's yeast including mechanical (bead mill, high pressure homogenization, ultrasonication), and non-mechanical (electrical, physical, chemical and enzymatic) techniques, as well as some newly developed methods. The advantages and drawbacks of different Cell Disruption methods were summarized by considering the energy consumption, the interaction of the Disruption methods with downstream operations and the process economics of alternative strategies. Finally, some future directions for research areas are proposed. Industrial relevance Wine making process entails the generation of significant amount of waste yeast, which represents an attractive source of valuable compounds that has been relatively unexploited to date. To retain the valuable Cell content, effective Cell Disruption strategies are needed to break the rigid yeast Cell walls. This review summarizes the state of the art of some emerging Cell Disruption techniques for recovery of intraCellular bio-active compounds from yeasts including mechanical (bead mill, high pressure homogenizer, ultrasonication), and non-mechanical (electrical, physical, chemical and enzymatic) techniques. Thereby, it identifies the process economics of alternative strategies by considering the interaction of the Disruption methods with downstream operations as well as the current situations and future research needs.
Paul A. Webley - One of the best experts on this subject based on the ideXlab platform.
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mechanical Cell Disruption for lipid extraction from microalgal biomass
Bioresource Technology, 2013Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. WebleyAbstract:Cell Disruption is an integral part of the downstream operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as Cell Disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of Cell Disruption followed a first-order model (0.65
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Mechanical Cell Disruption for lipid extraction from microalgal biomass.
Bioresource technology, 2013Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. WebleyAbstract:Cell Disruption is an integral part of the downstream operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as Cell Disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of Cell Disruption followed a first-order model (0.65
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Microalgal Cell Disruption for biofuel development
Applied Energy, 2012Co-Authors: Ronald Halim, Razif Harun, Michael K. Danquah, Paul A. WebleyAbstract:Abstract The production of alternative fuels from microalgae involves lengthy processing steps. Cell Disruption is an integral part of the downstream pool of unit operations as it facilitates the release of intraCellular products essential for biofuel production. This study investigated the use of high-pressure homogenization, ultrasonication, bead beating, and sulfuric acid treatment as laboratory-scale Disruption methods for microalgal Cells. The performance of each Cell Disruption method was evaluated in terms of two key indicators: reduction in the intact Cell count and reduction in the average colony diameter. The microalgal strain, Chlorococcum sp., was used throughout the study. The most effective Disruption was obtained using high-pressure homogenization (average Disruption = 73.8% of initial intact Cells) followed by sulfuric acid treatment (average Disruption = 33.2% of initial intact Cells) and bead beating (average Disruption = 17.5% of initial intact Cells). Even though ultrasonication failed to disrupt the microalgal Cells under the investigated conditions (average Disruption = 4.5% of initial intact Cells), it still managed to disintegrate Cellular colonies.
Mohamed Koubaa - One of the best experts on this subject based on the ideXlab platform.
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Current insights in yeast Cell Disruption technologies for oil recovery: A review
Chemical Engineering and Processing - Process Intensification, 2020Co-Authors: Mohamed Koubaa, Nabila Imatoukene, Lucie Drévillon, Eugène VorobievAbstract:Single Cell oils have been considered as potential replacers of plant and animal oils, and are mainly produced by yeasts, molds, and algae. Oleaginous yeasts have taken special attention due to the similarity of their metabolism with that of higher plants, and the numerous genetic tools available for their modification. However, a special need has been reported for efficient Cell Disruption technology due to the rigidity of their Cell wall compared to other biological ones. This makes the conventional maceration for oil recovery usually incomplete. Several mechanical and non-mechanical Cell Disruption technologies have been reported in the literature and are summarized in this review. A special focus is given for bead milling, high-pressure homogenization, ultrasound-, and microwave-assisted extraction. Their advantages and limitations, as well as the future potential strategies for oil recovery from oleaginous yeasts, are discussed.
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Cell Disruption pre-treatments towards an effective recovery of oil from Yarrowia lipolytica oleaginous yeast
Biomass and Bioenergy, 2019Co-Authors: Lucie Drévillon, Mohamed Koubaa, Jean-marc Nicaud, Eugène VorobievAbstract:The aim of this work was to evaluate the efficiency of different Cell Disruption techniques on oil extraction from Y. lipolytica oleaginous yeast. The techniques tested were mechanical expression (ME), moderate pulsed electric field assisted mechanical expression (MPEF-ME), high pulsed electric fields (HPEF), high voltage electrical discharges (HVED), ultrasound (US), and high-pressure homogenization (HPH). The impact of each Cell Disruption technique on fatty acid composition was investigated. Results show that the most efficient technique for Y. lipolytica Disruption was HPH leading to the obtaining of 83.8 ± 4.8% oil extraction yield, compared to only 19.8 ± 0.5% for the control, without any pre-treatment. In terms of fatty acid composition, HVED induced significant changes in almost all the analyzed fatty acids, while US was the softest technique. HPH, ME, and MPEF-ME induced changes in the content of some fatty acids.
Aniruddha B. Pandit - One of the best experts on this subject based on the ideXlab platform.
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Hydrodynamic cavitation for energy efficient and scalable process of microalgae Cell Disruption
Algal Research, 2019Co-Authors: Ashish G. Waghmare, Karuna Narsappa Nagula, Aniruddha B. Pandit, Shalini S. AryaAbstract:Abstract Microalgae are likely to emerge as a sustainable source of biomass which can be utilized as food, feed, and fuel. Most of the valuable products in the microalgae are present intraCellular. Microalgae Cell wall is rigid and mainly composed of Cellulose. Hence, it is essential to disrupt Cells to extract intraCellular products. Probe ultrasonication (US) and hydrodynamic cavitation (HC) were optimized for their operating parameters and various pre-treatments to obtain maximum Cell Disruption efficacy. Optimum conditions for maximum Cell Disruption using US were: solid load (1% w/v), duty cycle (80%), power input (54 W) and the US time 90 min. After pre-treatment with 0.5% w/v sodium hydroxide; the time required for the maximum Cell Disruption using US was reduced to 50 min. Optimum conditions for the maximum Cell Disruption using HC were cavitation device orifice over venturi, time (180 min), pressure (5 bar) and solid load (0.45% w/v). After pre-treatment with 0.5% w/v sodium hydroxide time required for maximum Cell Disruption using HC was reduced to 105 min. Further, the energy required for Cell Disruption using US and HC was calculated and, it was found that HC was significantly more energy efficient and scalable treatment for microalgae Cell Disruption.
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Application of Cavitational reactors for Cell Disruption for recovery of intraCellular enzymes
Journal of Chemical Technology & Biotechnology, 2008Co-Authors: Parag R. Gogate, Aniruddha B. PanditAbstract:Cavitational reactors are a novel and promising form of multiphase reactors, based on the principle of release of a large amount of energy owing to the violent collapse of cavities. This paper presents an overview of cavitational reactors in the specific area of Cell Disruption for the recovery of intraCellular enzymes, in terms of the basic aspects, different reactor configurations including recommendations for optimum operating parameters and review of earlier literature reports. It has been observed that under optimized conditions, cavitational reactors can reduce the energy requirement for the release of intraCellular enzymes by an order of magnitude compared with conventional Cell Disruption techniques used on an industrial scale. However, problems associated with efficient scale-up and operation at conditions required for industrial scale, hamper the successful utilization of cavitational reactors at this time. Some recommendations have been made for the future work required to realize the dream of harnessing the spectacular effects of cavitation phenomena.
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Comments on the Mechanism of Microbial Cell Disruption in High‐Pressure and High‐Speed Devices
Biotechnology progress, 1998Co-Authors: Irfan Z. Shirgaonkar, Rakesh R. Lothe, Aniruddha B. PanditAbstract:The dominant mechanism for microbial Cell Disruption in a high-pressure homogenizer and a high-speed homogenizer used in this study has been identified. It was found that the cavity collapse and the pressure pulse resulting from such a collapse have a significant influence on the rates of Cell Disruption. The similarities among the operating conditions for the decomposition of the aqueous KI solution to liberate iodine, the reaction occurring only under cavitating conditions, and that required for the substantial Disruption of microbial Cells have been pointed out. The liberation of iodine by the aqueous KI decomposition is treated as evidence of cavitation, and hence microbial Cell Disruption occurring at an identical discharge pressure confirms the mechanism of Cell Disruption as cavitation, in the high-pressure homogenizer valve. In the case of the high-speed homogenizer, shear and cavitation both play a significant role in Cell Disruption.
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Microbial Cell Disruption: role of cavitation
The Chemical Engineering Journal and the Biochemical Engineering Journal, 1994Co-Authors: S.s. Save, Aniruddha B. Pandit, Jyeshtharaj B. JoshiAbstract:Abstract A novel technique of using hydrodynamic cavitation for the large-scale Disruption of yeast Cells is described. Baker's yeast and brewer's yeast Cells in a pressed yeast form were used. Cell Disruption was monitored in the form of increase in soluble protein content. Disruption by hydrodynamic cavitation is compared with that obtained by established techniques such as blade blender and acoustic cavitation (ultrasonication). The effect of Cell concentration, time of treatment and number of passes in the flow loop system on the extent of Cell Disruption is reported. The energy efficiency of the hydrodynamic cavitation setup is compared with that of established techniques. Hydrodynamic cavitation was found to be at least an order of magnitude more energy efficient than established techniques such as ultrasonication or blade blender (mixer).
Ronald Halim - One of the best experts on this subject based on the ideXlab platform.
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mechanical Cell Disruption for lipid extraction from microalgal biomass
Bioresource Technology, 2013Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. WebleyAbstract:Cell Disruption is an integral part of the downstream operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as Cell Disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of Cell Disruption followed a first-order model (0.65
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Mechanical Cell Disruption for lipid extraction from microalgal biomass.
Bioresource technology, 2013Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. WebleyAbstract:Cell Disruption is an integral part of the downstream operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as Cell Disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of Cell Disruption followed a first-order model (0.65
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Microalgal Cell Disruption for biofuel development
Applied Energy, 2012Co-Authors: Ronald Halim, Razif Harun, Michael K. Danquah, Paul A. WebleyAbstract:Abstract The production of alternative fuels from microalgae involves lengthy processing steps. Cell Disruption is an integral part of the downstream pool of unit operations as it facilitates the release of intraCellular products essential for biofuel production. This study investigated the use of high-pressure homogenization, ultrasonication, bead beating, and sulfuric acid treatment as laboratory-scale Disruption methods for microalgal Cells. The performance of each Cell Disruption method was evaluated in terms of two key indicators: reduction in the intact Cell count and reduction in the average colony diameter. The microalgal strain, Chlorococcum sp., was used throughout the study. The most effective Disruption was obtained using high-pressure homogenization (average Disruption = 73.8% of initial intact Cells) followed by sulfuric acid treatment (average Disruption = 33.2% of initial intact Cells) and bead beating (average Disruption = 17.5% of initial intact Cells). Even though ultrasonication failed to disrupt the microalgal Cells under the investigated conditions (average Disruption = 4.5% of initial intact Cells), it still managed to disintegrate Cellular colonies.