The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform

Jianping Chen - One of the best experts on this subject based on the ideXlab platform.

  • spatholobus suberectus inhibits cancer Cell Growth by inducing apoptosis and arresting Cell cycle at g2 m checkpoint
    Journal of Ethnopharmacology, 2011
    Co-Authors: Zhi Yu Wang, Dongmei Wang, Yue Cheng, Leilei Chen, Jiangang Shen, Depo Yang, Louis Wingcheong Chow, Xin Yuan Guan, Jianping Chen
    Abstract:

    Abstract Aim of the study Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods The effect of SS on Cell proliferation was studied by Cell Growth Assay and flow cytometry on breast cancer Cell lines MCF-7 and colon cancer Cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation Assay and mitochondrial membrane potential Assay. Expression of proteins associated with Cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results Cell Growth Assay showed that SS effectively inhibits tumor Cell Growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the Cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential Assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor Growth via induction of apoptosis and arrest of the Cell cycle at G2/M phase.

  • Spatholobus suberectus inhibits cancer Cell Growth by inducing apoptosis and arresting Cell cycle at G2/M checkpoint.
    Journal of ethnopharmacology, 2010
    Co-Authors: Zhi Yu Wang, Dongmei Wang, Yue Cheng, Leilei Chen, Jiangang Shen, Depo Yang, Louis Wingcheong Chow, Xin Yuan Guan, Tjing Yung Loo, Jianping Chen
    Abstract:

    Abstract Aim of the study Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods The effect of SS on Cell proliferation was studied by Cell Growth Assay and flow cytometry on breast cancer Cell lines MCF-7 and colon cancer Cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation Assay and mitochondrial membrane potential Assay. Expression of proteins associated with Cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results Cell Growth Assay showed that SS effectively inhibits tumor Cell Growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the Cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential Assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor Growth via induction of apoptosis and arrest of the Cell cycle at G2/M phase.

B Berkhout - One of the best experts on this subject based on the ideXlab platform.

  • a competitive Cell Growth Assay for the detection of subtle effects of gene transduction on Cell proliferation
    Gene Therapy, 2012
    Co-Authors: Julia J M Eekels, Alexander O Pasternak, A M Schut, Dirk Geerts, Rienk E Jeeninga, B Berkhout
    Abstract:

    A competitive Cell Growth Assay for the detection of subtle effects of gene transduction on Cell proliferation

  • A competitive Cell Growth Assay for the detection of subtle effects of gene transduction on Cell proliferation
    Gene Therapy, 2012
    Co-Authors: Julia J M Eekels, Alexander O Pasternak, A M Schut, Dirk Geerts, Rienk E Jeeninga, B Berkhout
    Abstract:

    RNA interference (RNAi) is a sequence-specific gene silencing mechanism with therapeutic potential against many human pathogens. To obtain a durable therapeutic effect, stable transduction of target Cells with for instance a lentiviral vector that expresses a short hairpin (shRNA) inducer of the RNAi pathway is necessary. Apart from the intended therapeutic effect, this treatment can induce negative effects on Cell proliferation via off-target effects. A careful evaluation of the transduced Cells is required to develop a safe gene therapy approach. Stably transduced Cells are usually selected by expression of the enhanced green fluorescent protein (GFP) marker. In this study we show that the mixed transduction culture, containing both transduced GFP^+ and untransduced GFP^− Cells, can simply be passaged to score the GFP^+/GFP^− ratio by longitudinal flow cytometric analysis as a measure of the negative impact of the RNAi treatment on the Cellular proliferation rate. We show that this Assay is sensitive, easy to use and internally controlled for assessing subtle effects on Cell proliferation of lentiviral transduction and transgene expression.

Zhi Yu Wang - One of the best experts on this subject based on the ideXlab platform.

  • spatholobus suberectus inhibits cancer Cell Growth by inducing apoptosis and arresting Cell cycle at g2 m checkpoint
    Journal of Ethnopharmacology, 2011
    Co-Authors: Zhi Yu Wang, Dongmei Wang, Yue Cheng, Leilei Chen, Jiangang Shen, Depo Yang, Louis Wingcheong Chow, Xin Yuan Guan, Jianping Chen
    Abstract:

    Abstract Aim of the study Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods The effect of SS on Cell proliferation was studied by Cell Growth Assay and flow cytometry on breast cancer Cell lines MCF-7 and colon cancer Cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation Assay and mitochondrial membrane potential Assay. Expression of proteins associated with Cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results Cell Growth Assay showed that SS effectively inhibits tumor Cell Growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the Cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential Assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor Growth via induction of apoptosis and arrest of the Cell cycle at G2/M phase.

  • Spatholobus suberectus inhibits cancer Cell Growth by inducing apoptosis and arresting Cell cycle at G2/M checkpoint.
    Journal of ethnopharmacology, 2010
    Co-Authors: Zhi Yu Wang, Dongmei Wang, Yue Cheng, Leilei Chen, Jiangang Shen, Depo Yang, Louis Wingcheong Chow, Xin Yuan Guan, Tjing Yung Loo, Jianping Chen
    Abstract:

    Abstract Aim of the study Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods The effect of SS on Cell proliferation was studied by Cell Growth Assay and flow cytometry on breast cancer Cell lines MCF-7 and colon cancer Cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation Assay and mitochondrial membrane potential Assay. Expression of proteins associated with Cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results Cell Growth Assay showed that SS effectively inhibits tumor Cell Growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the Cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential Assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor Growth via induction of apoptosis and arrest of the Cell cycle at G2/M phase.

Julia J M Eekels - One of the best experts on this subject based on the ideXlab platform.

  • a competitive Cell Growth Assay for the detection of subtle effects of gene transduction on Cell proliferation
    Gene Therapy, 2012
    Co-Authors: Julia J M Eekels, Alexander O Pasternak, A M Schut, Dirk Geerts, Rienk E Jeeninga, B Berkhout
    Abstract:

    A competitive Cell Growth Assay for the detection of subtle effects of gene transduction on Cell proliferation

  • A competitive Cell Growth Assay for the detection of subtle effects of gene transduction on Cell proliferation
    Gene Therapy, 2012
    Co-Authors: Julia J M Eekels, Alexander O Pasternak, A M Schut, Dirk Geerts, Rienk E Jeeninga, B Berkhout
    Abstract:

    RNA interference (RNAi) is a sequence-specific gene silencing mechanism with therapeutic potential against many human pathogens. To obtain a durable therapeutic effect, stable transduction of target Cells with for instance a lentiviral vector that expresses a short hairpin (shRNA) inducer of the RNAi pathway is necessary. Apart from the intended therapeutic effect, this treatment can induce negative effects on Cell proliferation via off-target effects. A careful evaluation of the transduced Cells is required to develop a safe gene therapy approach. Stably transduced Cells are usually selected by expression of the enhanced green fluorescent protein (GFP) marker. In this study we show that the mixed transduction culture, containing both transduced GFP^+ and untransduced GFP^− Cells, can simply be passaged to score the GFP^+/GFP^− ratio by longitudinal flow cytometric analysis as a measure of the negative impact of the RNAi treatment on the Cellular proliferation rate. We show that this Assay is sensitive, easy to use and internally controlled for assessing subtle effects on Cell proliferation of lentiviral transduction and transgene expression.

Xin Yuan Guan - One of the best experts on this subject based on the ideXlab platform.

  • spatholobus suberectus inhibits cancer Cell Growth by inducing apoptosis and arresting Cell cycle at g2 m checkpoint
    Journal of Ethnopharmacology, 2011
    Co-Authors: Zhi Yu Wang, Dongmei Wang, Yue Cheng, Leilei Chen, Jiangang Shen, Depo Yang, Louis Wingcheong Chow, Xin Yuan Guan, Jianping Chen
    Abstract:

    Abstract Aim of the study Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods The effect of SS on Cell proliferation was studied by Cell Growth Assay and flow cytometry on breast cancer Cell lines MCF-7 and colon cancer Cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation Assay and mitochondrial membrane potential Assay. Expression of proteins associated with Cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results Cell Growth Assay showed that SS effectively inhibits tumor Cell Growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the Cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential Assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor Growth via induction of apoptosis and arrest of the Cell cycle at G2/M phase.

  • Spatholobus suberectus inhibits cancer Cell Growth by inducing apoptosis and arresting Cell cycle at G2/M checkpoint.
    Journal of ethnopharmacology, 2010
    Co-Authors: Zhi Yu Wang, Dongmei Wang, Yue Cheng, Leilei Chen, Jiangang Shen, Depo Yang, Louis Wingcheong Chow, Xin Yuan Guan, Tjing Yung Loo, Jianping Chen
    Abstract:

    Abstract Aim of the study Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods The effect of SS on Cell proliferation was studied by Cell Growth Assay and flow cytometry on breast cancer Cell lines MCF-7 and colon cancer Cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation Assay and mitochondrial membrane potential Assay. Expression of proteins associated with Cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results Cell Growth Assay showed that SS effectively inhibits tumor Cell Growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the Cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential Assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor Growth via induction of apoptosis and arrest of the Cell cycle at G2/M phase.