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Nobuaki Okamoto - One of the best experts on this subject based on the ideXlab platform.

  • Cell-Mediated Cytotoxicity in isogeneic ginbuna crucian carp
    Fish & Shellfish Immunology, 1999
    Co-Authors: T. Nakanishi, Nobuaki Okamoto
    Abstract:

    Abstract This review describes recent studies on Cell-Mediated Cytotoxicity of fish using clonal triploid ginbuna and discusses the exCellent potential of this species as a model for studying fish immunology. Graft-versus-host reaction (GVHR) followed by in vitro Cell-Mediated Cytotoxicity against allogeneic erythrocytes and Cell lines are described as specific cytotoxic activity in ginbuna. In vivo and in vitro Cell-Mediated Cytotoxicity obtained with ginbuna are characterised in terms of the requirement for repeated sensitisation of effector Cell donors to induce Cytotoxicity, killing of allogeneic recipients or Cells but not isogeneic targets by effector Cells, and the lack of involvement of ADCC. These results suggest that Cytotoxicity in ginbuna is Mediated by allo-reactive cytotoxic T Cells rather than non-specific cytotoxic Cells. Spontaneous cytotoxic activity of ginbuna neutrophils is also described with NK activity against human leukemic Cells and spontaneous killing of isogeneic erythrocytes.

  • Specific Cell-Mediated Cytotoxicity against an allogeneic target Cell line in isogeneic ginbuna crucian carp
    Fish & Shellfish Immunology, 1998
    Co-Authors: Satoshi Hasegawa, T. Nakanishi, Chihaya Nakayasu, Tomoyasu Yoshitomi, Nobuaki Okamoto
    Abstract:

    Specific Cell-Mediated Cytotoxicity in fish was investigated using both iso-geneic (clonal) ginbuna crucian carp,Carassius auratus langsdorfii, and three Cell lines established from the three different clones of this fish. Specific Cell-Mediated Cytotoxicity in ginbuna was induced as a result of intravenous immunisation with an allogenic Cell line, but not a syngeneic Cell line. As the cytotoxic activity tended to accelerate in proportion to the number of immunisations, specific cytotoxic Cells in ginbuna resembled mammalian cytotoxic T lymphocytes (CTL) in some respects. In addition, it was unlikely that antibody-dependent Cell-Mediated Cytotoxicity (ADCC) was involved in the Cytotoxicity observed in this study because ADCC activity of unimmunised fish effector Cells was not detected using the immunised ginbuna plasma or the supernatant of cultured effector Cells from immunised ginbuna. These results demonstrate that specific Cell-Mediated Cytotoxicity is inherent in fish and a set of clonal fish and their Cell lines may be useful for further studyin vivoandin vitro.

Jae U. Jung - One of the best experts on this subject based on the ideXlab platform.

  • Inhibition of natural killer Cell-Mediated Cytotoxicity by Kaposi's sarcoma-associated herpesvirus K5 protein.
    Immunity, 2000
    Co-Authors: Satoshi Ishido, Joong-kook Choi, Bok-soo Lee, Chunyang Wang, Maryann Demaria, R. Paul Johnson, George B. Cohen, Jae U. Jung
    Abstract:

    Abstract Kaposi's sarcoma–associated herpesvirus (KSHV) K3 and K5 proteins dramatically downregulate MHC class I molecules. However, although MHC class I downregulation may protect KSHV-infected Cells from cytotoxic T lymphocyte recognition, these Cells become potential targets for natural killer (NK) Cell–Mediated lysis. We now show that K5 also downregulates ICAM-1 and B7-2, which are ligands for NK Cell–Mediated Cytotoxicity receptors. As a consequence, K5 expression drastically inhibits NK Cell–Mediated Cytotoxicity. Conversely, de novo expression of B7-2 and ICAM-1 resensitizes the K5-expressing Cells to NK Cell–Mediated Cytotoxicity. This is a novel viral immune evasion strategy where KSHV K5 achieves immune avoidance by downregulation of Cellular ligands for NK Cell–Mediated Cytotoxicity receptors.

Subramaniam Malarkannan - One of the best experts on this subject based on the ideXlab platform.

  • In Vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection.
    Methods in molecular biology (Clifton N.J.), 2019
    Co-Authors: Nathan J. Schloemer, Alex M. Abel, Monica S. Thakar, Subramaniam Malarkannan
    Abstract:

    NK Cells are innate lymphocytes that are vital to clearance of virally infected or malignantly transformed Cells. Assessment of the cytotoxic response is an important component of NK Cell research and investigation of human disease. Standard assays of NK Cell-Mediated Cytotoxicity of CD107a degranulation or 51Cr release assay utilize cultured or freshly isolated NK Cell populations in vitro. In addition to requirements to maintain multiple target Cell lines and radioactivity precautions in the case of 51Cr, these are in vitro evaluations of a complex in vivo function. Here, we describe the in vivo assessment of NK Cell-Mediated Cytotoxicity through the adoptive transfer of splenocytes and their subsequent rejection. This protocol offers rapid, quantitative, and concurrent assessment of NK Cell-Mediated Cytotoxicity against the prototypic NK stimulations of "missing-self" and "nonself."

Hans-gustaf Ljunggren - One of the best experts on this subject based on the ideXlab platform.

  • Triggering of Natural Killer Cell Mediated Cytotoxicity by Costimulatory Molecules
    Current topics in microbiology and immunology, 1998
    Co-Authors: Benedict J. Chambers, Julia L. Wilson, Margarita Salcedo, K. Markovic, M. T. Bejarano, Hans-gustaf Ljunggren
    Abstract:

    Natural killer (NK) Cell Mediated Cytotoxicity is affected by both triggering and inhibitory signals (see e.g., Gumperz and Parham 1995; Lanier and Philips 1996; Raulet 1996). In several models target Cell major histocompatibility complex (MHC) class I molecules have been demonstrated to be able to turn off NK Cells (Ljunggren and Karre 1990; Karre 1995) by delivering inhibitory signals to MHC class I binding receptors (Karlhofer et al. 1992; Yokoyama and Seaman 1993). The latter include members of the Ly-49 receptors in mouse and the killer-Cell inhibitor receptors (KIR) in man (Gumperz and Parham 1995; Lanier and Philips 1996; Raulet 1996). While much attention has been focused on the role of MHC class I inhibition of NK Cell Mediated Cytotoxicity in recent years, significantly less attention has been devoted to receptor-ligand interactions that may trigger Cytotoxicity. This review discusses new insights into the ability of costimulatory molecules to trigger NK Cell Mediated Cytotoxicity.

Pierre Golstein - One of the best experts on this subject based on the ideXlab platform.

  • An early history of T Cell-Mediated Cytotoxicity
    Nature Reviews Immunology, 2018
    Co-Authors: Pierre Golstein, Gillian M. Griffiths
    Abstract:

    After 60 years of intense fundamental research into T Cell-Mediated Cytotoxicity, we have gained a detailed knowledge of the Cells involved, specific recognition mechanisms and post-recognition perforin–granzyme-based and FAS-based molecular mechanisms. What could not be anticipated at the outset was how discovery of the mechanisms regulating the activation and function of cytotoxic T Cells would lead to new developments in cancer immunotherapy. Given the profound recent interest in therapeutic manipulation of cytotoxic T Cell responses, it is an opportune time to look back on the early history of the field. This Timeline describes how the early findings occurred and eventually led to current therapeutic applications. This Timeline looks back at the past 60 years of fundamental research into the mechanisms of T Cell-Mediated Cytotoxicity, which has culminated in recent interest in the therapeutic manipulation of cytotoxic T Cell responses for cancer immunotherapy.

  • An early history of T Cell-Mediated Cytotoxicity
    Nature Reviews Immunology, 2018
    Co-Authors: Pierre Golstein, Gillian M. Griffiths
    Abstract:

    After 60 years of intense fundamental research into T Cell-Mediated Cytotoxicity, we have gained a detailed knowledge of the Cells involved, specific recognition mechanisms and post-recognition perforin-granzyme-based and FAS-based molecular mechanisms. What could not be anticipated at the outset was how discovery of the mechanisms regulating the activation and function of cytotoxic T Cells would lead to new developments in cancer immunotherapy. Given the profound recent interest in therapeutic manipulation of cytotoxic T Cell responses, it is an opportune time to look back on the early history of the field. This Timeline describes how the early findings occurred and eventually led to current therapeutic applications.

  • fas and perforin pathways as major mechanisms of t Cell Mediated Cytotoxicity
    Science, 1994
    Co-Authors: David Kagi, Francoise Vignaux, Valerie Depraetere, Birgit Ledermann, Hans Hengartner, Kurt Bürki, Shigekazu Nagata, Pierre Golstein
    Abstract:

    Two molecular mechanisms of T Cell-Mediated Cytotoxicity, one perforin-based, the other Fas-based, have been demonstrated. To determine the extent of their contribution to T Cell-Mediated Cytotoxicity, a range of effector Cells from normal control or perforin-deficient mice were tested against a panel of target Cells with various levels of Fas expression. All Cytotoxicity observed was due to either of these mechanisms, and no third mechanism was detected. Thus, the perforin- and Fas-based mechanisms may account for all T Cell-Mediated Cytotoxicity in short-term in vitro assays.

  • fas involvement in ca 2 independent t Cell Mediated Cytotoxicity
    Journal of Experimental Medicine, 1993
    Co-Authors: E Rouvier, Mariefrancoise Luciani, Pierre Golstein
    Abstract:

    Mechanisms of T Cell-Mediated Cytotoxicity remain poorly defined at the molecular level. To investigate some of these mechanisms, we used as target Cells, on the one hand, thymocytes from lpr and gld mouse mutants, and on the other hand, L1210 Cells transfected or not with the apoptosis-inducing Fas molecule. These independent mutant or transfectant-based approaches both led to the conclusion that Fas was involved in the Ca(2+)-independent component of Cytotoxicity Mediated by at least two sources of T Cells, namely nonantigen-specific in vitro activated hybridoma Cells, and antigen-specific in vivo raised peritoneal exudate lymphocytes. Thus, in these cases, T Cell-Mediated Cytotoxicity involved transduction via Fas of the target Cell death signal.