The Experts below are selected from a list of 162 Experts worldwide ranked by ideXlab platform
Lihua Sui - One of the best experts on this subject based on the ideXlab platform.
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Cell death pathway induced by resveratrol-bovine serum albumin nanoparticles in a human ovarian Cell line.
Oncology letters, 2015Co-Authors: Liyuan Guo, Yan Peng, Jingping Yao, Jing Wang, Guang-mei Zhang, Jie Chen, Lihua SuiAbstract:Resveratrol-bovine serum albumin nanoparticles (RES-BSANP) exhibit chemotherapeutic properties, which trigger apoptosis. The aim of the present study was to investigate the caspase-independent Cell death pathway induced by RES-BSANP in human ovarian cancer SKOV3 Cells and to analyze its mechanism. Morphological changes were observed by apoptotic body/Cell Nucleus DNA staining using inverted and fluorescence microscopy. The Cell death pathway was determined by phosphatidylserine translocation. Western blot analysis was conducted to detect the activation of apoptosis-inducing factor (AIF), cytochrome c (Cyto c) and B-Cell lymphoma 2-associated X protein (Bax). Apoptotic body and nuclear condensation and fragmentation were observed simultaneously following treatment with RES-BSANP. RES-BSANP induced apoptosis in a dose-dependent manner in the human ovarian cancer SKOV3 Cells. The translocation of AIF from the mitochondria to the cytoplasm occurred earlier than that of Cyto c. In addition, Bax binding to the mitochondria was required for the release of AIF and Cyto c from the mitochondria. The AIF apoptosis pathway may present an alternative caspase-dependent apoptosis pathway in human ovarian Cell death induced by RES-BSANP. Elucidation of this pathway may be critical for the treatment of cancer using high doses of RES-BSANP.
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Mechanisms of resveratrol bovine serum albumin nanoparticle-induced Cell death in human ovarian cancer SKOV3 Cells
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2010Co-Authors: Liyuan Guo, Yan Peng, Jingping Yao, Jing Wang, Guang-mei Zhang, Jie Chen, Lihua SuiAbstract:OBJECTIVE To study the effect of resveratrol bovine serum albumin nanoparticles on SKOV3 Cell line and its mechanisms. METHODS The morphological changes of the Cells exposed to the nanoparticles were observed by apoptotic body/Cell Nucleus DNA staining under inverted microscope and fluorescence microscope, and the pathway of Cell death was determined by phosphatidylserine translocation. Western blotting was performed to detect the activation of cyto.c, caspase-3 and caspase-9. RESULTS DNA ladder was detected with gel electrophoresis and the Cell death was partially inhibited by the pan-caspase inhibitor Z-VAD-FMK. Gel electrophoresis displayed both DNA ladder and smear in RES-BSANP exposed groups, while DNA ladder disappeared in Z-VAD-FMK group and only the smear was left. Cyto.c in the cytoplasm was released at 2 h, while the expression of caspase-9 protein reached the peak level at 4 h and caspase-3 expression was obvious enhanced at 8 h. At 4 h, caspase-9 expression in the Cells exposed to 100 µmol/L RES-BSANP was decreased significantly as compared to the Cells treated with 50 µmol/L RES-BSANP (P>0.05). CONCLUSION RES-BSANP can induce the necrosis and apoptosis of SKOV3 Cells via either caspase-dependent or caspase-independent pathways.
Carlos Velez-pardo - One of the best experts on this subject based on the ideXlab platform.
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Cannabinoid CP55940 selectively induces apoptosis in Jurkat Cells and in ex vivo T-Cell acute lymphoblastic leukemia through H2O2 signaling mechanism
Leukemia research, 2020Co-Authors: Viviana Soto-mercado, Miguel Mendivil-perez, Marlene Jimenez-del-rio, Javier E. Fox, Carlos Velez-pardoAbstract:Abstract T-Cell acute lymphoblastic leukemia (T-ALL) is a highly heterogeneous malignant hematological disorder arising from T-Cell progenitors. This study was aimed to evaluate the cytotoxic effect of CP55940 on human peripheral blood lymphocytes (PBL) and on T-ALL Cells (Jurkat). PBL and Jurkat Cells were treated with CP55940 (0−20 μM), and morphological changes in the Cell Nucleus/ DNA, mitochondrial membrane potential (ΔΨm), and intraCellular reactive oxygen species levels were determined by fluorescence microscopy and flow cytometry. Cellular apoptosis markers were also evaluated by western blotting, pharmacological inhibition and immunofluorescence. CP55940 induced apoptotic Cell death in Jurkat Cells, but not in PBL, in a dose–response manner with increasing fragmentation of DNA, arrest of Cell cycle and damage of ΔΨm. CP55940 increased dichlorofluorescein fluorescence (DCF) intensity, increased DJ-1 Cys106- sulfonate, a marker of intraCellular stress, induced the up-regulation of p53 and phosphorylation of transcription factor c-JUN. It increased the expression of BAX and PUMA, up-regulated mitochondrial proteins PINK1 and Parkin, and activated CASPASE-3. Antioxidant NAC, pifithrin-α, and SP600125 blocked CP55940 deleterious effect on Jurkat Cells. However, the potent and highly specific cannabinoid CB1 and CB2 receptor inverse agonist SR141716 and SR144528 were unable to blunt CP55940-induced apoptosis in Jurkat Cells. Conclusively CP55940 provokes Cell death in Jurkat through CBR-independent mechanism. Interestingly, CP55940 was also cytotoxic to ex vivo T-ALL Cells from chemotherapy-resistant pediatric patients. In conclusion, CP55940 selectively induces apoptosis in Jurkat Cells through a H2O2-mediated signaling pathway. Our findings support the use of cannabinoids as a potential treatment for T-ALL Cells.
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L-amino acid oxidase isolated from Micrurus mipartitus snake venom (MipLAAO) specifically induces apoptosis in acute lymphoblastic leukemia Cells mostly via oxidative stress-dependent signaling mechanism.
International journal of biological macromolecules, 2019Co-Authors: Jesus Bedoya-medina, Miguel Mendivil-perez, Paola Rey-suárez, Marlene Jimenez-del-rio, Vitelbina Núñez, Carlos Velez-pardoAbstract:Abstract The effect of Micrurus mipartitus snake venom as a therapeutic alternative for T-acute lymphoblastic leukemia (ALL) is still unknown. This study was aimed to evaluate the cytotoxic effect of M. mipartitus snake venom and a new L-amino acid oxidase (LAAO), named MipLAAO, on human peripheral blood lymphocytes (PBL) and on T-ALL Cells (Jurkat), and its mechanism of action. PBL and Jurkat Cells were treated with venom and MipLAAO, and morphological changes in the Cell Nucleus/DNA, mitochondrial membrane potential, levels of intraCellular reactive oxygen species and Cellular apoptosis markers were determined by fluorescence microscopy, flow cytometry and pharmacological inhibition. Venom and MipLAAO induced apoptotic Cell death in Jurkat Cells, but not in PBL, in a dose-response manner. Additionally, venom and MipLAAO increased dichlorofluorescein fluorescence intensity, indicative of H2O2 production, increased DJ-1 Cys106-sulfonate, as a marker of intraCellular stress and induced the up-regulation of PUMA, p53 and phosphorylation of c-JUN. Additionally, it increased the expression of apoptotic CASPASE-3. In conclusion, M. mipartitus venom and MipLAAO selectively induces apoptosis in Jurkat Cells through a H2O2-mediated signaling pathway dependent mostly on CASPASE-3 pathway. Our findings support the potential use of M. mipartitus snake venom compounds as a potential treatment for T-ALL.
Liang Yun-dan - One of the best experts on this subject based on the ideXlab platform.
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Application of Nuclear DNA of Enamel and Dentin in Personal Identification
Journal of Kunming Medical University, 2009Co-Authors: Liang Yun-danAbstract:Objective To evaluate the application of nuclear DNA of enamel and dentin in personal identification.Methods We extracted nuclear DNA from enamel and dentin,because enamel and dentin had little influence in physical and chemical factors.We collected 20 molars from 20 irrelevant persons.Results The traditional organic extraction method extracted Cell nuclear DNA from Enamel and dentin,and obtained favourable Cell Nucleus DNA. Conclusion Nuclear DNA obtained from enamel and dentin provide important evidence for case.
Jacqueline K. Barton - One of the best experts on this subject based on the ideXlab platform.
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Evidence for DNA charge transport in the Nucleus.
Biochemistry, 2001Co-Authors: Megan E. Núñez, Gerald P. Holmquist, Jacqueline K. BartonAbstract:Oxidative damage to DNA bases in isolated HeLa nuclei occurs upon treatment with rhodium intercalators and photoactivation. Oxidation occurs preferentially at the 5‘-guanine of 5‘-GG-3‘ sites, indicative of base damage by DNA-mediated charge transfer chemistry. Moreover, oxidative damage occurs at protein-bound sites which are inaccessible to rhodium. Thus, on transcriptionally active DNA within the Cell Nucleus, DNA-mediated charge transport leads to base damage from a distance, and direct interaction of an oxidant is not necessary to generate a base lesion at a specific site. These observations require consideration in designing new chemotherapeutics and in understanding Cellular mechanisms for DNA damage and repair.
Liyuan Guo - One of the best experts on this subject based on the ideXlab platform.
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Cell death pathway induced by resveratrol-bovine serum albumin nanoparticles in a human ovarian Cell line.
Oncology letters, 2015Co-Authors: Liyuan Guo, Yan Peng, Jingping Yao, Jing Wang, Guang-mei Zhang, Jie Chen, Lihua SuiAbstract:Resveratrol-bovine serum albumin nanoparticles (RES-BSANP) exhibit chemotherapeutic properties, which trigger apoptosis. The aim of the present study was to investigate the caspase-independent Cell death pathway induced by RES-BSANP in human ovarian cancer SKOV3 Cells and to analyze its mechanism. Morphological changes were observed by apoptotic body/Cell Nucleus DNA staining using inverted and fluorescence microscopy. The Cell death pathway was determined by phosphatidylserine translocation. Western blot analysis was conducted to detect the activation of apoptosis-inducing factor (AIF), cytochrome c (Cyto c) and B-Cell lymphoma 2-associated X protein (Bax). Apoptotic body and nuclear condensation and fragmentation were observed simultaneously following treatment with RES-BSANP. RES-BSANP induced apoptosis in a dose-dependent manner in the human ovarian cancer SKOV3 Cells. The translocation of AIF from the mitochondria to the cytoplasm occurred earlier than that of Cyto c. In addition, Bax binding to the mitochondria was required for the release of AIF and Cyto c from the mitochondria. The AIF apoptosis pathway may present an alternative caspase-dependent apoptosis pathway in human ovarian Cell death induced by RES-BSANP. Elucidation of this pathway may be critical for the treatment of cancer using high doses of RES-BSANP.
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Mechanisms of resveratrol bovine serum albumin nanoparticle-induced Cell death in human ovarian cancer SKOV3 Cells
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2010Co-Authors: Liyuan Guo, Yan Peng, Jingping Yao, Jing Wang, Guang-mei Zhang, Jie Chen, Lihua SuiAbstract:OBJECTIVE To study the effect of resveratrol bovine serum albumin nanoparticles on SKOV3 Cell line and its mechanisms. METHODS The morphological changes of the Cells exposed to the nanoparticles were observed by apoptotic body/Cell Nucleus DNA staining under inverted microscope and fluorescence microscope, and the pathway of Cell death was determined by phosphatidylserine translocation. Western blotting was performed to detect the activation of cyto.c, caspase-3 and caspase-9. RESULTS DNA ladder was detected with gel electrophoresis and the Cell death was partially inhibited by the pan-caspase inhibitor Z-VAD-FMK. Gel electrophoresis displayed both DNA ladder and smear in RES-BSANP exposed groups, while DNA ladder disappeared in Z-VAD-FMK group and only the smear was left. Cyto.c in the cytoplasm was released at 2 h, while the expression of caspase-9 protein reached the peak level at 4 h and caspase-3 expression was obvious enhanced at 8 h. At 4 h, caspase-9 expression in the Cells exposed to 100 µmol/L RES-BSANP was decreased significantly as compared to the Cells treated with 50 µmol/L RES-BSANP (P>0.05). CONCLUSION RES-BSANP can induce the necrosis and apoptosis of SKOV3 Cells via either caspase-dependent or caspase-independent pathways.