The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Waghray G - One of the best experts on this subject based on the ideXlab platform.
-
Use of the University of Wisconsin solution for the preservation of Cell Organelle activities in rat heart and lungs.
Journal of Heart and Lung Transplantation, 1995Co-Authors: Rosalind S. Labow, Meek E, Waghray GAbstract:BACKGROUND The University of Wisconsin storage solution has been successful in some model systems in extending the storage period of heart-lung grafts for transplantation. METHODS In this study an aerated preparation of rat heart and lung was stored for 24 hours at 4 degrees C in either University of Wisconsin or St. Thomas' Hospital solution. Cell Organelles (reticular, mitochondrial, and Cell membrane fractions) were isolated from the stored hearts and lungs. Protein yield and enzyme activities were assayed for each Cell Organelle (reticular fractions: Ca(2+)-ATPase, NADPH-cytochrome C reductase; mitochondria: Ca(2+)-ATPase, cytochrome C oxidase; Cell membrane fraction: Na+,K(+)-ATPase, p-nitrophenylphosphatase) as a measure of the recovery of function. RESULTS Only the Cell membrane fraction of heart and lung was not affected by storage in either St. Thomas' Hospital or University of Wisconsin solution with respect to protein yield (milligrams per gram of homogenate) or enzyme activities (nanomole per milligram per minute). The reticular fraction was the most sensitive to storage, with both protein yield and enzyme activities being significantly reduced in both the heart and the lung stored in University of Wisconsin of St. Thomas' Hospital solution (p < 0.05). CONCLUSION The mitochondrial fraction was not preserved in lung in either St. Thomas' Hospital or University of Wisconsin solution but was preserved in the heart stored in St. Thomas' Hospital solution. These criteria provide preliminary screening for a superior solution that may then be used in more complicated transplantation models to more fully assess cardiac and pulmonary function.
-
Preservation of Cell Organelles during storage of human atrial tissue in the University of Wisconsin solution.
The Journal of cardiovascular surgery, 1995Co-Authors: Rosalind S. Labow, Waghray G, E Meek, Paul J. Hendry, Wilbert J. KeonAbstract:AIM OF THE STUDY The University of Wisconsin storage solution (UW) (E.I. du Pont de Nemours, Wilmington, DE) has been successful in extending the storage period using some model systems of donor heart preservation for cardiac transplantation. The ability of UW to preserve human cardiac Cell Organelle (sarcoplasmic reticulum, mitochondria and sarcolemmal) membrane composition (enzyme activity, protein, cholesterol and phospholipid content) was compared to St. Thomas's Hospital Solution (ST) and saline. METHODS Human atrial appendages were stored at 4 degrees C for 24 h in saline, ST or UW or not stored (controls) and the Cell Organelles isolated. Each fraction was assayed for enzyme activity (mitochondria: azide sensitive Ca2+ ATPase, cytochrome C oxidase; sarcolemmal membrane: Na+K+ ATPase, p-nitrophenylphosphatase; sarcoplasmic reticulum: CA2+ uptake, Ca2+ ATPase, NADPH cytochrome C reductase), protein, cholesterol and phospholipid content. RESULTS "Protein yield" proved to be the most sensitive marker for Cell Organelle preservation. Only the sarcolemmal membrane showed no decrease in either enzyme activities of "protein yield" after storage in saline, ST or UW. Mitochondria showed no decrease in enzyme activities but a decrease in "protein yield" after storage in all 3 solutions. The "protein yield" of sarcoplasmic reticulum was significantly reduced after storage in UW, saline and ST. No correlation could be drawn between cholesterol and phospholipid content and the preservation of Cell Organelle function. CONCLUSIONS It is possible to distinguish between the ability of solutions to preserve the membrane composition of human cardiac tissue during hypothermic storage. Using simple assays to assess preservation provides preliminary screening for a superior solution which can then be used in more complicated transplantation models to more fully assess cardiac function.
Rosalind S. Labow - One of the best experts on this subject based on the ideXlab platform.
-
Use of the University of Wisconsin solution for the preservation of Cell Organelle activities in rat heart and lungs.
Journal of Heart and Lung Transplantation, 1995Co-Authors: Rosalind S. Labow, Meek E, Waghray GAbstract:BACKGROUND The University of Wisconsin storage solution has been successful in some model systems in extending the storage period of heart-lung grafts for transplantation. METHODS In this study an aerated preparation of rat heart and lung was stored for 24 hours at 4 degrees C in either University of Wisconsin or St. Thomas' Hospital solution. Cell Organelles (reticular, mitochondrial, and Cell membrane fractions) were isolated from the stored hearts and lungs. Protein yield and enzyme activities were assayed for each Cell Organelle (reticular fractions: Ca(2+)-ATPase, NADPH-cytochrome C reductase; mitochondria: Ca(2+)-ATPase, cytochrome C oxidase; Cell membrane fraction: Na+,K(+)-ATPase, p-nitrophenylphosphatase) as a measure of the recovery of function. RESULTS Only the Cell membrane fraction of heart and lung was not affected by storage in either St. Thomas' Hospital or University of Wisconsin solution with respect to protein yield (milligrams per gram of homogenate) or enzyme activities (nanomole per milligram per minute). The reticular fraction was the most sensitive to storage, with both protein yield and enzyme activities being significantly reduced in both the heart and the lung stored in University of Wisconsin of St. Thomas' Hospital solution (p < 0.05). CONCLUSION The mitochondrial fraction was not preserved in lung in either St. Thomas' Hospital or University of Wisconsin solution but was preserved in the heart stored in St. Thomas' Hospital solution. These criteria provide preliminary screening for a superior solution that may then be used in more complicated transplantation models to more fully assess cardiac and pulmonary function.
-
Use of the University of Wisconsin solution for the preservation of Cell Organelle activities in rat heart and lungs.
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, 1995Co-Authors: Rosalind S. Labow, E Meek, G WaghrayAbstract:The University of Wisconsin storage solution has been successful in some model systems in extending the storage period of heart-lung grafts for transplantation. In this study an aerated preparation of rat heart and lung was stored for 24 hours at 4 degrees C in either University of Wisconsin or St. Thomas' Hospital solution. Cell Organelles (reticular, mitochondrial, and Cell membrane fractions) were isolated from the stored hearts and lungs. Protein yield and enzyme activities were assayed for each Cell Organelle (reticular fractions: Ca(2+)-ATPase, NADPH-cytochrome C reductase; mitochondria: Ca(2+)-ATPase, cytochrome C oxidase; Cell membrane fraction: Na+,K(+)-ATPase, p-nitrophenylphosphatase) as a measure of the recovery of function. Only the Cell membrane fraction of heart and lung was not affected by storage in either St. Thomas' Hospital or University of Wisconsin solution with respect to protein yield (milligrams per gram of homogenate) or enzyme activities (nanomole per milligram per minute). The reticular fraction was the most sensitive to storage, with both protein yield and enzyme activities being significantly reduced in both the heart and the lung stored in University of Wisconsin of St. Thomas' Hospital solution (p < 0.05). The mitochondrial fraction was not preserved in lung in either St. Thomas' Hospital or University of Wisconsin solution but was preserved in the heart stored in St. Thomas' Hospital solution. These criteria provide preliminary screening for a superior solution that may then be used in more complicated transplantation models to more fully assess cardiac and pulmonary function.
-
Preservation of Cell Organelles during storage of human atrial tissue in the University of Wisconsin solution.
The Journal of cardiovascular surgery, 1995Co-Authors: Rosalind S. Labow, Waghray G, E Meek, Paul J. Hendry, Wilbert J. KeonAbstract:AIM OF THE STUDY The University of Wisconsin storage solution (UW) (E.I. du Pont de Nemours, Wilmington, DE) has been successful in extending the storage period using some model systems of donor heart preservation for cardiac transplantation. The ability of UW to preserve human cardiac Cell Organelle (sarcoplasmic reticulum, mitochondria and sarcolemmal) membrane composition (enzyme activity, protein, cholesterol and phospholipid content) was compared to St. Thomas's Hospital Solution (ST) and saline. METHODS Human atrial appendages were stored at 4 degrees C for 24 h in saline, ST or UW or not stored (controls) and the Cell Organelles isolated. Each fraction was assayed for enzyme activity (mitochondria: azide sensitive Ca2+ ATPase, cytochrome C oxidase; sarcolemmal membrane: Na+K+ ATPase, p-nitrophenylphosphatase; sarcoplasmic reticulum: CA2+ uptake, Ca2+ ATPase, NADPH cytochrome C reductase), protein, cholesterol and phospholipid content. RESULTS "Protein yield" proved to be the most sensitive marker for Cell Organelle preservation. Only the sarcolemmal membrane showed no decrease in either enzyme activities of "protein yield" after storage in saline, ST or UW. Mitochondria showed no decrease in enzyme activities but a decrease in "protein yield" after storage in all 3 solutions. The "protein yield" of sarcoplasmic reticulum was significantly reduced after storage in UW, saline and ST. No correlation could be drawn between cholesterol and phospholipid content and the preservation of Cell Organelle function. CONCLUSIONS It is possible to distinguish between the ability of solutions to preserve the membrane composition of human cardiac tissue during hypothermic storage. Using simple assays to assess preservation provides preliminary screening for a superior solution which can then be used in more complicated transplantation models to more fully assess cardiac function.
Petr Vaňhara - One of the best experts on this subject based on the ideXlab platform.
-
Alleviation of endoplasmic reticulum stress by tauroursodeoxycholic acid delays senescence of mouse ovarian surface epithelium.
Cell and tissue research, 2018Co-Authors: Kateřina Vašíčková, Lukáš Moráň, Dominik Gurín, Petr VaňharaAbstract:Ovarian surface epithelium (OSE) forms a single layer of mostly cuboidal Cells on surface of mammalian ovaries that is inherently exposed to Cell stress evoked by tissue damage every ovulation and declines morphologically after menopause. Endoplasmic reticulum (ER) is a principal Cell Organelle involved in proteosynthesis, but also integrating various stress signals. ER stress evokes a conserved signaling pathway, the unfolded protein response (UPR), leading to Cell death or adaptation to stress conditions. In this work, we document that mouse OSE suffers from ER stress during replicative senescence in vitro, develops abnormalities in ER and initiates UPR. Attenuation of ER stress in senescent OSE by tauroursodeoxycholic acid (TUDCA) reconditions ER architecture and leads to delayed onset of senescence. In summary, we show for the first time a mutual molecular link between ER stress response and replicative senescence leading to phenotypic changes of non-malignant ovarian surface epithelium.
Pedro M. Domingos - One of the best experts on this subject based on the ideXlab platform.
-
Cellular responses to endoplasmic reticulum stress and apoptosis
Apoptosis, 2009Co-Authors: Vanya I. Rasheva, Pedro M. DomingosAbstract:The endoplasmic reticulum (ER) is the Cell Organelle where secretory and membrane proteins are synthesized and folded. Correctly folded proteins exit the ER and are transported to the Golgi and other destinations within the Cell, but proteins that fail to fold properly—misfolded proteins—are retained in the ER and their accumulation may constitute a form of stress to the Cell—ER stress. Several signaling pathways, collectively known as unfolded protein response (UPR), have evolved to detect the accumulation of misfolded proteins in the ER and activate a Cellular response that attempts to maintain homeostasis and a normal flux of proteins in the ER. In certain severe situations of ER stress, however, the protective mechanisms activated by the UPR are not sufficient to restore normal ER function and Cells die by apoptosis. Most research on the UPR used yeast or mammalian model systems and only recently Drosophila has emerged as a system to study the molecular and Cellular mechanisms of the UPR. Here, we review recent advances in Drosophila UPR research, in the broad context of mammalian and yeast literature.
Setsuko Komatsu - One of the best experts on this subject based on the ideXlab platform.
-
Plant Cell Organelle Proteomics in Response to Abiotic Stress
Journal of proteome research, 2011Co-Authors: Zahed Hossain, Mohammad-zaman Nouri, Setsuko KomatsuAbstract:Proteomics is one of the finest molecular techniques extensively being used for the study of protein profiling of a given plant species experiencing stressed conditions. Plants respond to a stress by alteration in the pattern of protein expression, either by up-regulating of the existing protein pool or by the synthesizing novel proteins primarily associated with plants antioxidative defense mechanism. Improved protein extraction protocols and advance techniques for identification of novel proteins have been standardized in different plant species at both Cellular and whole plant level for better understanding of abiotic stress sensing and intraCellular stress signal transduction mechanisms. In contrast, an in-depth proteome study of subCellular Organelles could generate much detail information about the intrinsic mechanism of stress response as it correlates the possible relationship between the protein abundance and plant stress tolerance. Although a wealth of reviews devoted to plant proteomics are available, review articles dedicated to plant Cell Organelle proteins response under abiotic stress are very scanty. In the present review, an attempt has been made to summarize all significant contributions related to abiotic stresses and their impacts on Organelle proteomes for better understanding of plants abiotic stress tolerance mechanism at protein level. This review will not only provide new insights into the plants stress response mechanisms, which are necessary for future development of genetically engineered stress tolerant crop plants for the benefit of humankind, but will also highlight the importance of studying changes in protein abundance within the Cell Organelles in response to abiotic stress.