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Thomas A Rando - One of the best experts on this subject based on the ideXlab platform.

  • heterogeneity in the muscle satellite Cell Population
    Seminars in Cell & Developmental Biology, 2010
    Co-Authors: Stefano Biressi, Thomas A Rando
    Abstract:

    Satellite Cells, the adult stem Cells responsible for skeletal muscle regeneration, are defined by their location between the basal lamina and the fiber sarcolemma. Increasing evidence suggests that satellite Cells represent a heterogeneous Population of Cells with distinct embryological origin and multiple levels of biochemical and functional diversity. This review focuses on the rich diversity of the satellite Cell Population based on studies across species. Ultimately, a more complete characterization of the heterogeneity of satellite Cells will be essential to understand the functional significance in terms of muscle growth, homeostasis, tissue repair, and aging.

Nikos V. Mantzaris - One of the best experts on this subject based on the ideXlab platform.

  • from single Cell genetic architecture to Cell Population dynamics quantitatively decomposing the effects of different Population heterogeneity sources for a genetic network with positive feedback architecture
    Biophysical Journal, 2007
    Co-Authors: Nikos V. Mantzaris
    Abstract:

    Phenotypic Cell-to-Cell variability or Cell Population heterogeneity originates from two fundamentally different sources: unequal partitioning of Cellular material at Cell division and stochastic fluctuations associated with intraCellular reactions. We developed a mathematical and computational framework that can quantitatively isolate both heterogeneity sources and applied it to a genetic network with positive feedback architecture. The framework consists of three vastly different mathematical formulations: a), a continuum model, which completely neglects Population heterogeneity; b), a deterministic Cell Population balance model, which accounts for Population heterogeneity originating only from unequal partitioning at Cell division; and c), a fully stochastic model accommodating both sources of Population heterogeneity. The framework enables the quantitative decomposition of the effects of the different Population heterogeneity sources on system behavior. Our results indicate the importance of Cell Population heterogeneity in accurately predicting even average Population properties. Moreover, we find that unequal partitioning at Cell division and sharp division rates shrink the region of the parameter space where the Population exhibits bistable behavior, a characteristic feature of networks with positive feedback architecture. In addition, intrinsic noise at the single-Cell level due to slow operator fluctuations and small numbers of molecules further contributes toward the shrinkage of the bistability regime at the Cell Population level. Finally, the effect of intrinsic noise at the Cell Population level was found to be markedly different than at the single-Cell level, emphasizing the importance of simulating entire Cell Populations and not just individual Cells to understand the complex interplay between single-Cell genetic architecture and behavior at the Cell Population level.

  • Single-Cell gene-switching networks and heterogeneous Cell Population phenotypes
    Computers & Chemical Engineering, 2005
    Co-Authors: Nikos V. Mantzaris
    Abstract:

    Abstract Cell Populations are heterogeneous systems in the sense that Cellular properties are unevenly distributed amongst the Cells of the Population. In this work, we present a novel methodological and computational framework for the quantitative assessment of the effects of Cell Population heterogeneity on the dynamics of Cell Populations. It combines the Cell Population balance modeling approach which accounts for Cell Population heterogeneity with the continuum modeling approach, which does not. We focus on Populations of Cells carrying an artificial genetic network, known as the genetic toggle, consisting of a system of two promoter–repressor pairs. Detailed numerical simulations indicate that taking into account Cell Population heterogeneity leads to agreement with experimental data, while neglecting it results in significant qualitative and quantitative differences both transiently and at balanced growth. Furthermore, the simulations revealed the effect of systemic parameters on both the transient and balanced growth behavior, leading us to the formulation of specific hypotheses to interpret experimental results. Thus, Cell Population balance modeling when used in conjunction with mechanistic single-Cell models constitutes a powerful predictive tool which can provide invaluable quantitative insights into the biological system under consideration.

  • Effects of Cell Population Heterogeneity on the Dynamics of Cell Populations
    IFAC Proceedings Volumes, 2004
    Co-Authors: Nikos V. Mantzaris
    Abstract:

    Abstract A novel methodological and computational framework for the quantitative assessment of the effects of Cell Population heterogeneity on the dynamics of Cell Populations is presented. We focus on Populations of Cells carrying an artificial genetic network, consisting of a system of two promoterrepressor pairs. Detailed numerical simulations indicate that taking into account Cell Population heterogeneity leads to agreement with experimental data, while neglecting it results in significant qualitative and quantitative differences both transiently and at balanced growth.

Kwang-sup Soh - One of the best experts on this subject based on the ideXlab platform.

  • Spontaneous Ultraweak Photon Emission during the Growth of the Cell Population of Cultured HeLa Cell Line
    JOURNAL OF HEALTH SCIENCE, 2007
    Co-Authors: Jungdae Kim, Yong-ung Kim, Youngjoo Lee, Masaki Kobayashi, Yuji Tsutsumi, Ryuichiro Kondo, Seung Ki Lee, Kwang-sup Soh
    Abstract:

    We analyzed the change in the intensities of spontaneous ultraweak photon emission during the growth of the Cell Population of HeLa Cell line using a Cell culture system. Comparisons of the temporal change of intensities of spontaneous ultraweak photon emission with that of the growth curve showed that the intensity followe da lmost thesame course with the change rate kinetics of Cell Population. The analysis of Cell viability during the growth of the Cell Population indicated that there is a close relationship between the intensities of photon emission and proliferative rate of viable Cells. The flow-cytometric analysis indicated that the intensities of the photon emission are related primarily to the 2n diploid Cell equivalent Populations. We suggest that the spontaneous ultraweak photon emission is mainly involved in the changes of the ploidy number during the proliferative process of the cancer Cell line. The therapeutic effects of acupuncture may be explained scientifically by the spontaneous ultraweak photon emissions from the Cells during the changes of its ploidy number in th ea natomical structure of acupuncture meridians.

  • Analysis of Changes in the Intensities of Spontaneous Ultraweak Photon Emission during the Growth of the Cell Population of Cultured HeLa Cell Line
    World Congress on Medical Physics and Biomedical Engineering 2006, 1
    Co-Authors: Jungdae Kim, Yong-ung Kim, Youngjoo Lee, Ryuichiro Kondo, Seung Ki Lee, Jaehong Han, Hyeran You, Kwang-sup Soh
    Abstract:

    We analyzed the change in the intensities of spontaneous ultraweak photon emission during the growth of the Cell Population of HeLa Cell line using a Cell culture system. Comparisons of the temporal change of intensities of spontaneous ultraweak photon emission with that of the growth curve showed that the intensity followed almost the same course with the change rate kinetics of Cell Population. The analysis of Cell viability and proliferation during the growth of the Cell Population indicated that there is a close relationship between the intensities of the photon emission and proliferative rate of viable HeLa Cells. We suggest that the spontaneous ultraweak photon emission is mainly originated from the metabolic reactions involved in the proliferative process of Cells.

Richard L Lieber - One of the best experts on this subject based on the ideXlab platform.

  • reduced satellite Cell Population may lead to contractures in children with cerebral palsy
    Developmental Medicine & Child Neurology, 2013
    Co-Authors: Lucas R Smith, Henry G Chambers, Richard L Lieber
    Abstract:

    Aim  Satellite Cells are the stem Cells residing in muscle responsible for skeletal muscle growth and repair. Skeletal muscle in cerebral palsy (CP) has impaired longitudinal growth that results in muscle contractures. We hypothesized that the satellite Cell Population would be reduced in contractured muscle. Method  We compared the satellite Cell Populations in hamstring muscles from participants with CP contracture (n=8; six males, two females; age range 6–15y; Gross Motor Function Classification System [GMFCS] levels II–V; 4 with hemiplegia, 4 with diplegia) and from typically developing participants (n=8; six males, two females, age range 15–18y). Muscle biopsies were extracted from the gracilis and semitendinosus muscles and mononuclear Cells were isolated. Cell surface markers were stained with fluorescently conjugated antibodies to label satellite Cells (neural Cell adhesion molecule) and inflammatory and endothelial Cells (CD34 and CD4 respectively). Cells were analyzed using flow cytometry to determine Cell Populations. Results  After gating for intact Cells a mean of 12.8% (SD 2.8%) were determined to be satellite Cells in typically developing children, but only 5.3% (SD 2.3%; p 0.05) suggesting the isolation procedure was valid. Interpretation  A reduced satellite Cell Population may account for the decreased longitudinal growth of muscles in CP that develop into fixed contractures or the decreased ability to strengthen muscle in CP. This suggests a unique musculoskeletal disease mechanism and provides a potential therapeutic target for debilitating muscle contractures.

Jungdae Kim - One of the best experts on this subject based on the ideXlab platform.

  • Spontaneous Ultraweak Photon Emission during the Growth of the Cell Population of Cultured HeLa Cell Line
    JOURNAL OF HEALTH SCIENCE, 2007
    Co-Authors: Jungdae Kim, Yong-ung Kim, Youngjoo Lee, Masaki Kobayashi, Yuji Tsutsumi, Ryuichiro Kondo, Seung Ki Lee, Kwang-sup Soh
    Abstract:

    We analyzed the change in the intensities of spontaneous ultraweak photon emission during the growth of the Cell Population of HeLa Cell line using a Cell culture system. Comparisons of the temporal change of intensities of spontaneous ultraweak photon emission with that of the growth curve showed that the intensity followe da lmost thesame course with the change rate kinetics of Cell Population. The analysis of Cell viability during the growth of the Cell Population indicated that there is a close relationship between the intensities of photon emission and proliferative rate of viable Cells. The flow-cytometric analysis indicated that the intensities of the photon emission are related primarily to the 2n diploid Cell equivalent Populations. We suggest that the spontaneous ultraweak photon emission is mainly involved in the changes of the ploidy number during the proliferative process of the cancer Cell line. The therapeutic effects of acupuncture may be explained scientifically by the spontaneous ultraweak photon emissions from the Cells during the changes of its ploidy number in th ea natomical structure of acupuncture meridians.

  • Analysis of Changes in the Intensities of Spontaneous Ultraweak Photon Emission during the Growth of the Cell Population of Cultured HeLa Cell Line
    World Congress on Medical Physics and Biomedical Engineering 2006, 1
    Co-Authors: Jungdae Kim, Yong-ung Kim, Youngjoo Lee, Ryuichiro Kondo, Seung Ki Lee, Jaehong Han, Hyeran You, Kwang-sup Soh
    Abstract:

    We analyzed the change in the intensities of spontaneous ultraweak photon emission during the growth of the Cell Population of HeLa Cell line using a Cell culture system. Comparisons of the temporal change of intensities of spontaneous ultraweak photon emission with that of the growth curve showed that the intensity followed almost the same course with the change rate kinetics of Cell Population. The analysis of Cell viability and proliferation during the growth of the Cell Population indicated that there is a close relationship between the intensities of the photon emission and proliferative rate of viable HeLa Cells. We suggest that the spontaneous ultraweak photon emission is mainly originated from the metabolic reactions involved in the proliferative process of Cells.