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Ali G. Turhan - One of the best experts on this subject based on the ideXlab platform.
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Quantitative and qualitative in vitro analysis of the stem Cell Potential of hematopoietic Cells purified from murine skeletal muscle
Cell research, 2007Co-Authors: Celine Haond, Françoise Farace, Martine Guillier, Yann Lécluse, Frédéric Mazurier, William Vainchenker, Ali G. TurhanAbstract:Quantitative and qualitative in vitro analysis of the stem Cell Potential of hematopoietic Cells purified from murine skeletal muscle
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Quantitative and qualitative in vitro analysis of the stem Cell Potential of hematopoietic Cells purified from murine skeletal muscle
Cell Research, 2007Co-Authors: Celine Haond, Françoise Farace, Martine Guillier, Yann Lécluse, Frédéric Mazurier, William Vainchenker, Ali G. TurhanAbstract:The murine skeletal muscle contains hematopoietic stem Cells, but this Potential has so far not been studied quantitatively or qualitatively in vitro . To quantify the hematopoietic stem Cell Potential, we have used highly purified SP/CD45^+ Cells in long-term culture initiating Cell (LTC-IC) assays. The SP/CD45^+ Cell population purified from murine muscle was found to have significant stem Cell activity with an LTC-IC frequency of 1/640. Single-Cell-sorted SP/CD45^+ Cells from muscle exhibited robust proliferative activity in vitro at day 16 (380-fold amplification), especially after culture with OP-9 layers that also support embryonic stem Cells. Amplified Cell populations originating from single Cells exhibited multilineage differentiation ability with evidence of myeloid, lymphoid and NK Cell markers. Thus, our results demonstrate that hematopoietic stem Cells that can be quantified by LTC-IC assays exist in the murine skeletal muscle and show also for the first time, at the single-Cell level, that these Cells exhibit multilineage differentiation ability and major proliferative Potential.
Kenneth L Noller - One of the best experts on this subject based on the ideXlab platform.
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correlation of umbilical cord blood hormones and growth factors with stem Cell Potential implications for the prenatal origin of breast cancer hypothesis
Breast Cancer Research, 2007Co-Authors: Todd M Savarese, Inkyung Baik, William C. Okulicz, Qin Liu, Pagona Lagiou, William C Strohsnitter, Hoi Pang Low, David Chelmow, Peter J Quesenberry, Kenneth L NollerAbstract:Introduction Prenatal levels of mitogens may influence the lifetime breast cancer risk by driving stem Cell proliferation and increasing the number of target Cells, and thereby increasing the chance of mutation events that initiate oncogenesis. We examined in umbilical cord blood the correlation of Potential breast epithelial mitogens, including hormones and growth factors, with hematopoietic stem Cell concentrations serving as surrogates of overall stem Cell Potential.
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Correlation of umbilical cord blood hormones and growth factors with stem Cell Potential: implications for the prenatal origin of breast cancer hypothesis
Breast Cancer Research, 2007Co-Authors: Todd M Savarese, Inkyung Baik, Qin Liu, Pagona Lagiou, William C Strohsnitter, Hoi Pang Low, David Chelmow, Peter J Quesenberry, William Okulicz, Kenneth L NollerAbstract:Introduction Prenatal levels of mitogens may influence the lifetime breast cancer risk by driving stem Cell proliferation and increasing the number of target Cells, and thereby increasing the chance of mutation events that initiate oncogenesis. We examined in umbilical cord blood the correlation of Potential breast epithelial mitogens, including hormones and growth factors, with hematopoietic stem Cell concentrations serving as surrogates of overall stem Cell Potential. Methods We analyzed cord blood samples from 289 deliveries. Levels of hormones and growth factors were correlated with concentrations of stem Cell and progenitor populations (CD34^+ Cells, CD34^+CD38^- Cells, CD34^+c-kit^+ Cells, and granulocyte–macrophage colony-forming units). Changes in stem Cell concentration associated with each standard deviation change in mitogens and the associated 95% confidence intervals were calculated from multiple regression analysis. Results Cord blood plasma levels of insulin-like growth factor-1 (IGF-1) were strongly correlated with all the hematopoietic stem and progenitor concentrations examined (one standard-deviation increase in IGF-1 being associated with a 15–19% increase in stem/progenitor concentrations, all P < 0.02). Estriol and insulin-like growth factor binding protein-3 levels were positively and significantly correlated with some of these Cell populations. Sex hormone-binding globulin levels were negatively correlated with these stem/progenitor pools. These relationships were stronger in Caucasians and Hispanics and were weaker or not present in Asian-Americans and African-Americans. Conclusion Our data support the concept that in utero mitogens may drive the expansion of stem Cell populations. The correlations with IGF-1 and estrogen are noteworthy, as both are crucial for mammary gland development.
Inkyung Baik - One of the best experts on this subject based on the ideXlab platform.
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Correlation of umbilical cord blood hormones and growth factors with stem Cell Potential: implications for the prenatal origin of breast cancer hypothesis
Breast Cancer Research, 2007Co-Authors: Todd M Savarese, Inkyung Baik, Qin Liu, Pagona Lagiou, William C Strohsnitter, Hoi Pang Low, David Chelmow, Peter J Quesenberry, William Okulicz, Kenneth L NollerAbstract:Introduction Prenatal levels of mitogens may influence the lifetime breast cancer risk by driving stem Cell proliferation and increasing the number of target Cells, and thereby increasing the chance of mutation events that initiate oncogenesis. We examined in umbilical cord blood the correlation of Potential breast epithelial mitogens, including hormones and growth factors, with hematopoietic stem Cell concentrations serving as surrogates of overall stem Cell Potential. Methods We analyzed cord blood samples from 289 deliveries. Levels of hormones and growth factors were correlated with concentrations of stem Cell and progenitor populations (CD34^+ Cells, CD34^+CD38^- Cells, CD34^+c-kit^+ Cells, and granulocyte–macrophage colony-forming units). Changes in stem Cell concentration associated with each standard deviation change in mitogens and the associated 95% confidence intervals were calculated from multiple regression analysis. Results Cord blood plasma levels of insulin-like growth factor-1 (IGF-1) were strongly correlated with all the hematopoietic stem and progenitor concentrations examined (one standard-deviation increase in IGF-1 being associated with a 15–19% increase in stem/progenitor concentrations, all P < 0.02). Estriol and insulin-like growth factor binding protein-3 levels were positively and significantly correlated with some of these Cell populations. Sex hormone-binding globulin levels were negatively correlated with these stem/progenitor pools. These relationships were stronger in Caucasians and Hispanics and were weaker or not present in Asian-Americans and African-Americans. Conclusion Our data support the concept that in utero mitogens may drive the expansion of stem Cell populations. The correlations with IGF-1 and estrogen are noteworthy, as both are crucial for mammary gland development.
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correlation of umbilical cord blood hormones and growth factors with stem Cell Potential implications for the prenatal origin of breast cancer hypothesis
Breast Cancer Research, 2007Co-Authors: Todd M Savarese, Inkyung Baik, William C. Okulicz, Qin Liu, Pagona Lagiou, William C Strohsnitter, Hoi Pang Low, David Chelmow, Peter J Quesenberry, Kenneth L NollerAbstract:Introduction Prenatal levels of mitogens may influence the lifetime breast cancer risk by driving stem Cell proliferation and increasing the number of target Cells, and thereby increasing the chance of mutation events that initiate oncogenesis. We examined in umbilical cord blood the correlation of Potential breast epithelial mitogens, including hormones and growth factors, with hematopoietic stem Cell concentrations serving as surrogates of overall stem Cell Potential.
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Association of fetal hormone levels with stem Cell Potential: evidence for early life roots of human cancer
Cancer research, 2005Co-Authors: Inkyung Baik, William J. Devito, Karen K. Ballen, Pamela S. Becker, William C. Okulicz, Qin Liu, Ellen Delpapa, Pagona Lagiou, Susan R. Sturgeon, Dimitrios TrichopoulosAbstract:Intrauterine and perinatal factors have been linked to risk of childhood leukemia, testicular cancer, and breast cancer in the offspring. The pool of stem Cells in target tissue has been suggested as a critical factor linking early life exposures to cancer. We examined the relation between intrauterine hormone levels and measurements of stem Cell Potential in umbilical cord blood. Cord blood donors were 40 women, ages ≥18 years, who delivered, from August 2002 to June 2003, a singleton birth after a gestation of at least 37 weeks. We assayed plasma concentrations of estradiol, unconjugated estriol, testosterone, progesterone, prolactin, sex hormone binding globulin, insulin-like growth factor-I (IGF-I), and IGF binding protein-3. For stem Cell Potential, we measured concentrations of CD34 + and CD34 + CD38 − Cells and granulocyte-macrophage colony-forming unit (CFU-GM). We applied linear regression analysis and controlled for maternal and neonatal characteristics. We found strong positive associations between IGF-I and stem Cell measures, 1 SD increase in IGF-I being associated with a 41% increase in CD34 + ( P = 0.008), a 109% increase in CD34 + CD38 − ( P = 0.005), and a 94% increase in CFU-GM ( P = 0.01). Similar associations were observed for IGF binding protein-3. Among steroid hormones, estriol and testosterone were significantly positively associated with CD34 + and CFU-GM. These findings indicate that levels of growth factors and hormones are strongly associated with stem Cell Potential in human umbilical cord blood and point to a Potential mechanism that may mediate the relationship between in utero exposure to hormones and cancer risk in the offspring.
Celine Haond - One of the best experts on this subject based on the ideXlab platform.
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Quantitative and qualitative in vitro analysis of the stem Cell Potential of hematopoietic Cells purified from murine skeletal muscle
Cell research, 2007Co-Authors: Celine Haond, Françoise Farace, Martine Guillier, Yann Lécluse, Frédéric Mazurier, William Vainchenker, Ali G. TurhanAbstract:Quantitative and qualitative in vitro analysis of the stem Cell Potential of hematopoietic Cells purified from murine skeletal muscle
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Quantitative and qualitative in vitro analysis of the stem Cell Potential of hematopoietic Cells purified from murine skeletal muscle
Cell Research, 2007Co-Authors: Celine Haond, Françoise Farace, Martine Guillier, Yann Lécluse, Frédéric Mazurier, William Vainchenker, Ali G. TurhanAbstract:The murine skeletal muscle contains hematopoietic stem Cells, but this Potential has so far not been studied quantitatively or qualitatively in vitro . To quantify the hematopoietic stem Cell Potential, we have used highly purified SP/CD45^+ Cells in long-term culture initiating Cell (LTC-IC) assays. The SP/CD45^+ Cell population purified from murine muscle was found to have significant stem Cell activity with an LTC-IC frequency of 1/640. Single-Cell-sorted SP/CD45^+ Cells from muscle exhibited robust proliferative activity in vitro at day 16 (380-fold amplification), especially after culture with OP-9 layers that also support embryonic stem Cells. Amplified Cell populations originating from single Cells exhibited multilineage differentiation ability with evidence of myeloid, lymphoid and NK Cell markers. Thus, our results demonstrate that hematopoietic stem Cells that can be quantified by LTC-IC assays exist in the murine skeletal muscle and show also for the first time, at the single-Cell level, that these Cells exhibit multilineage differentiation ability and major proliferative Potential.
Qin Liu - One of the best experts on this subject based on the ideXlab platform.
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Correlation of umbilical cord blood hormones and growth factors with stem Cell Potential: implications for the prenatal origin of breast cancer hypothesis
Breast Cancer Research, 2007Co-Authors: Todd M Savarese, Inkyung Baik, Qin Liu, Pagona Lagiou, William C Strohsnitter, Hoi Pang Low, David Chelmow, Peter J Quesenberry, William Okulicz, Kenneth L NollerAbstract:Introduction Prenatal levels of mitogens may influence the lifetime breast cancer risk by driving stem Cell proliferation and increasing the number of target Cells, and thereby increasing the chance of mutation events that initiate oncogenesis. We examined in umbilical cord blood the correlation of Potential breast epithelial mitogens, including hormones and growth factors, with hematopoietic stem Cell concentrations serving as surrogates of overall stem Cell Potential. Methods We analyzed cord blood samples from 289 deliveries. Levels of hormones and growth factors were correlated with concentrations of stem Cell and progenitor populations (CD34^+ Cells, CD34^+CD38^- Cells, CD34^+c-kit^+ Cells, and granulocyte–macrophage colony-forming units). Changes in stem Cell concentration associated with each standard deviation change in mitogens and the associated 95% confidence intervals were calculated from multiple regression analysis. Results Cord blood plasma levels of insulin-like growth factor-1 (IGF-1) were strongly correlated with all the hematopoietic stem and progenitor concentrations examined (one standard-deviation increase in IGF-1 being associated with a 15–19% increase in stem/progenitor concentrations, all P < 0.02). Estriol and insulin-like growth factor binding protein-3 levels were positively and significantly correlated with some of these Cell populations. Sex hormone-binding globulin levels were negatively correlated with these stem/progenitor pools. These relationships were stronger in Caucasians and Hispanics and were weaker or not present in Asian-Americans and African-Americans. Conclusion Our data support the concept that in utero mitogens may drive the expansion of stem Cell populations. The correlations with IGF-1 and estrogen are noteworthy, as both are crucial for mammary gland development.
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correlation of umbilical cord blood hormones and growth factors with stem Cell Potential implications for the prenatal origin of breast cancer hypothesis
Breast Cancer Research, 2007Co-Authors: Todd M Savarese, Inkyung Baik, William C. Okulicz, Qin Liu, Pagona Lagiou, William C Strohsnitter, Hoi Pang Low, David Chelmow, Peter J Quesenberry, Kenneth L NollerAbstract:Introduction Prenatal levels of mitogens may influence the lifetime breast cancer risk by driving stem Cell proliferation and increasing the number of target Cells, and thereby increasing the chance of mutation events that initiate oncogenesis. We examined in umbilical cord blood the correlation of Potential breast epithelial mitogens, including hormones and growth factors, with hematopoietic stem Cell concentrations serving as surrogates of overall stem Cell Potential.
-
Association of fetal hormone levels with stem Cell Potential: evidence for early life roots of human cancer
Cancer research, 2005Co-Authors: Inkyung Baik, William J. Devito, Karen K. Ballen, Pamela S. Becker, William C. Okulicz, Qin Liu, Ellen Delpapa, Pagona Lagiou, Susan R. Sturgeon, Dimitrios TrichopoulosAbstract:Intrauterine and perinatal factors have been linked to risk of childhood leukemia, testicular cancer, and breast cancer in the offspring. The pool of stem Cells in target tissue has been suggested as a critical factor linking early life exposures to cancer. We examined the relation between intrauterine hormone levels and measurements of stem Cell Potential in umbilical cord blood. Cord blood donors were 40 women, ages ≥18 years, who delivered, from August 2002 to June 2003, a singleton birth after a gestation of at least 37 weeks. We assayed plasma concentrations of estradiol, unconjugated estriol, testosterone, progesterone, prolactin, sex hormone binding globulin, insulin-like growth factor-I (IGF-I), and IGF binding protein-3. For stem Cell Potential, we measured concentrations of CD34 + and CD34 + CD38 − Cells and granulocyte-macrophage colony-forming unit (CFU-GM). We applied linear regression analysis and controlled for maternal and neonatal characteristics. We found strong positive associations between IGF-I and stem Cell measures, 1 SD increase in IGF-I being associated with a 41% increase in CD34 + ( P = 0.008), a 109% increase in CD34 + CD38 − ( P = 0.005), and a 94% increase in CFU-GM ( P = 0.01). Similar associations were observed for IGF binding protein-3. Among steroid hormones, estriol and testosterone were significantly positively associated with CD34 + and CFU-GM. These findings indicate that levels of growth factors and hormones are strongly associated with stem Cell Potential in human umbilical cord blood and point to a Potential mechanism that may mediate the relationship between in utero exposure to hormones and cancer risk in the offspring.