The Experts below are selected from a list of 317493 Experts worldwide ranked by ideXlab platform

Javier Alvarez - One of the best experts on this subject based on the ideXlab platform.

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Chromaffin-Cell Stimulation triggers fast millimolar mitochondrial Ca 2+ transients that modulate secretion

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Activation of calcium-ion (Ca2+) channels on the plasma membrane and on intraCellular Ca2+ stores, such as the endoplasmic reticulum, generates local transient increases in the cytosolic Ca2+ concentration that induce Ca2+ uptake by neighbouring mitochondria. Here, by using mitochondrially targeted aequorin proteins with different Ca2+ affinities, we show that half of the chromaffin-Cell mitochondria exhibit surprisingly rapid millimolar Ca2+ transients upon Stimulation of Cells with acetylcholine, caffeine or high concentrations of potassium ions. Our results show a tight functional coupling of voltage-dependent Ca2+ channels on the plasma membrane, ryanodine receptors on the endoplasmic reticulum, and mitochondria. Cell Stimulation generates localized Ca2+ transients, with Ca2+ concentrations above 20-40 microM, at these functional units. Protonophores abolish mitochondrial Ca2+ uptake and increase stimulated secretion of catecholamines by three- to fivefold. These results indicate that mitochondria modulate secretion by controlling the availability of Ca2+ for exocytosis.

Mayte Montero - One of the best experts on this subject based on the ideXlab platform.

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Chromaffin-Cell Stimulation triggers fast millimolar mitochondrial Ca 2+ transients that modulate secretion

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Activation of calcium-ion (Ca2+) channels on the plasma membrane and on intraCellular Ca2+ stores, such as the endoplasmic reticulum, generates local transient increases in the cytosolic Ca2+ concentration that induce Ca2+ uptake by neighbouring mitochondria. Here, by using mitochondrially targeted aequorin proteins with different Ca2+ affinities, we show that half of the chromaffin-Cell mitochondria exhibit surprisingly rapid millimolar Ca2+ transients upon Stimulation of Cells with acetylcholine, caffeine or high concentrations of potassium ions. Our results show a tight functional coupling of voltage-dependent Ca2+ channels on the plasma membrane, ryanodine receptors on the endoplasmic reticulum, and mitochondria. Cell Stimulation generates localized Ca2+ transients, with Ca2+ concentrations above 20-40 microM, at these functional units. Protonophores abolish mitochondrial Ca2+ uptake and increase stimulated secretion of catecholamines by three- to fivefold. These results indicate that mitochondria modulate secretion by controlling the availability of Ca2+ for exocytosis.

Antonio G Garcia - One of the best experts on this subject based on the ideXlab platform.

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Chromaffin-Cell Stimulation triggers fast millimolar mitochondrial Ca 2+ transients that modulate secretion

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Activation of calcium-ion (Ca2+) channels on the plasma membrane and on intraCellular Ca2+ stores, such as the endoplasmic reticulum, generates local transient increases in the cytosolic Ca2+ concentration that induce Ca2+ uptake by neighbouring mitochondria. Here, by using mitochondrially targeted aequorin proteins with different Ca2+ affinities, we show that half of the chromaffin-Cell mitochondria exhibit surprisingly rapid millimolar Ca2+ transients upon Stimulation of Cells with acetylcholine, caffeine or high concentrations of potassium ions. Our results show a tight functional coupling of voltage-dependent Ca2+ channels on the plasma membrane, ryanodine receptors on the endoplasmic reticulum, and mitochondria. Cell Stimulation generates localized Ca2+ transients, with Ca2+ concentrations above 20-40 microM, at these functional units. Protonophores abolish mitochondrial Ca2+ uptake and increase stimulated secretion of catecholamines by three- to fivefold. These results indicate that mitochondria modulate secretion by controlling the availability of Ca2+ for exocytosis.

Inmaculada Cuchilloibanez - One of the best experts on this subject based on the ideXlab platform.

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Chromaffin-Cell Stimulation triggers fast millimolar mitochondrial Ca 2+ transients that modulate secretion

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Activation of calcium-ion (Ca2+) channels on the plasma membrane and on intraCellular Ca2+ stores, such as the endoplasmic reticulum, generates local transient increases in the cytosolic Ca2+ concentration that induce Ca2+ uptake by neighbouring mitochondria. Here, by using mitochondrially targeted aequorin proteins with different Ca2+ affinities, we show that half of the chromaffin-Cell mitochondria exhibit surprisingly rapid millimolar Ca2+ transients upon Stimulation of Cells with acetylcholine, caffeine or high concentrations of potassium ions. Our results show a tight functional coupling of voltage-dependent Ca2+ channels on the plasma membrane, ryanodine receptors on the endoplasmic reticulum, and mitochondria. Cell Stimulation generates localized Ca2+ transients, with Ca2+ concentrations above 20-40 microM, at these functional units. Protonophores abolish mitochondrial Ca2+ uptake and increase stimulated secretion of catecholamines by three- to fivefold. These results indicate that mitochondria modulate secretion by controlling the availability of Ca2+ for exocytosis.

Maria Teresa Alonso - One of the best experts on this subject based on the ideXlab platform.

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Chromaffin-Cell Stimulation triggers fast millimolar mitochondrial Ca 2+ transients that modulate secretion

  • chromaffin Cell Stimulation triggers fast millimolar mitochondrial ca2 transients that modulate secretion
    Nature Cell Biology, 2000
    Co-Authors: Mayte Montero, Estela Carnicero, Inmaculada Cuchilloibanez, Almudena Albillos, Antonio G Garcia, Javier Garciasancho, Maria Teresa Alonso, Javier Alvarez
    Abstract:

    Activation of calcium-ion (Ca2+) channels on the plasma membrane and on intraCellular Ca2+ stores, such as the endoplasmic reticulum, generates local transient increases in the cytosolic Ca2+ concentration that induce Ca2+ uptake by neighbouring mitochondria. Here, by using mitochondrially targeted aequorin proteins with different Ca2+ affinities, we show that half of the chromaffin-Cell mitochondria exhibit surprisingly rapid millimolar Ca2+ transients upon Stimulation of Cells with acetylcholine, caffeine or high concentrations of potassium ions. Our results show a tight functional coupling of voltage-dependent Ca2+ channels on the plasma membrane, ryanodine receptors on the endoplasmic reticulum, and mitochondria. Cell Stimulation generates localized Ca2+ transients, with Ca2+ concentrations above 20-40 microM, at these functional units. Protonophores abolish mitochondrial Ca2+ uptake and increase stimulated secretion of catecholamines by three- to fivefold. These results indicate that mitochondria modulate secretion by controlling the availability of Ca2+ for exocytosis.