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Erik C Neyts - One of the best experts on this subject based on the ideXlab platform.

  • plasma induced destruction of bacterial Cell Wall Components a reactive molecular dynamics simulation
    Journal of Physical Chemistry C, 2013
    Co-Authors: Maksudbek Yusupov, Annemie Bogaerts, Stijn Huygh, Ramses Snoeckx, Adri C T Van Duin, Erik C Neyts
    Abstract:

    Nonthermal atmospheric pressure plasmas are gaining increasing attention for biomedical applications. However, very little fundamental information on the interaction mechanisms between the plasma species and biological Cells is currently available. We investigate the interaction of important plasma species, such as OH, H2O2, O, O3, as well as O2 and H2O, with bacterial peptidoglycan by means of reactive molecular dynamics simulations, aiming for a better understanding of plasma disinfection. Our results show that OH, O, O3, and H2O2 can break structurally important bonds of peptidoglycan (i.e., C–O, C–N, or C–C bonds), which consequently leads to the destruction of the bacterial Cell Wall. The mechanisms behind these breakups are, however, dependent on the impinging plasma species, and this also determines the effectiveness of the Cell Wall destruction.

  • plasma induced destruction of bacterial Cell Wall Components a reactive molecular dynamics simulation
    Journal of Physical Chemistry C, 2013
    Co-Authors: Maksudbek Yusupov, Annemie Bogaerts, Stijn Huygh, Ramses Snoeckx, Adri C T Van Duin, Erik C Neyts
    Abstract:

    Nonthermal atmospheric pressure plasmas are gaining increasing attention for biomedical applications. However, very little fundamental information on the interaction mechanisms between the plasma s...

Chris P. Chanway - One of the best experts on this subject based on the ideXlab platform.

  • substrate utilization by endophytic bacteria paenibacillus polymyxa p2b 2r that may facilitate bacterial entrance and survival inside diverse plant hosts
    FACETS, 2017
    Co-Authors: Henry Yang, Akshit Puri, Kiran Preet Padda, Chris P. Chanway
    Abstract:

    Bacterial endophytes are thought to enter plants either through pre-existing openings in plant tissues or by creating openings by hydrolyzing major plant Cell Wall Components. A lodgepole endophyte...

  • Substrate utilization by endophytic bacteria Paenibacillus polymyxa P2b-2R that may facilitate bacterial entrance and survival inside diverse plant hosts
    Canadian Science Publishing, 2017
    Co-Authors: Henry Yang, Akshit Puri, Kiran Preet Padda, Chris P. Chanway
    Abstract:

    Bacterial endophytes are thought to enter plants either through pre-existing openings in plant tissues or by creating openings by hydrolyzing major plant Cell Wall Components. A lodgepole endophyte, Paenibacillus polymyxa P2b-2R, consistently formed endophytic colonies when inoculated in diverse plant hosts, viz., lodgepole pine, western red cedar, corn, canola, and tomato. We were interested to know, whether or not this bacterial strain possesses enzymes that can hydrolyze three major plant Cell Wall Components namely Cellulose, xylan, and pectin to facilitate entrance into the host plants. Using a BIOLOG assay, we also tested this bacterial strain’s ability to utilize carbon sources that might facilitate its entrance and hence its survival inside host plants. Paenibacillus polymyxa P2b-2R hydrolyzed sodium carboxymethylCellulose, beechwood xylan, and sodium polypectate and utilized 39 of the 95 carbon sources (41%) tested. Of the 39 carbon substrates oxidized by P2b-2R, the “carbohydrates” group represents the largest source of utilizable carbon (23 out of 39). Thus, it can be concluded that P. polymyxa P2b-2R is able to degrade major Cell Wall Components (Cellulose, xylan, and pectin) and utilize some of the available carbon substrates, possibly to gain entry and survive inside the plant and form endophytic colonies thereafter

Keith W Waldron - One of the best experts on this subject based on the ideXlab platform.

  • characterization of Cell Wall Components of wheat bran following hydrothermal pretreatment and fractionation
    Biotechnology for Biofuels, 2015
    Co-Authors: Zara Merali, Samuel R A Collins, Adam Elliston, David R Wilson, Andres Kasper, Keith W Waldron
    Abstract:

    Background Pretreatments are a prerequisite for enzymatic hydrolysis of biomass and production of ethanol. They are considered to open up the plant Cell Wall structure by altering, moving or solubilizing lignin and hydrolyzing a proportion of hemiCellulosic moieties. However, there is little information concerning pretreatment-induced changes on wheat bran Cell Wall polymers and indeed on changes in Cell Wall phenolic esters in bran or other lignoCellulosic biomass. Here, we evaluate polymeric changes (chemical and physical) as a result of selected hydrothermal pretreatment conditions on destarched wheat bran using controlled polymer extraction methods. Quantification of Cell Wall Components together with soluble oligosaccharides, the insoluble residues and ease of extractability and fractionation of biomass residues were conducted.

  • characterization of Cell Wall Components of wheat straw following hydrothermal pretreatment and fractionation
    Bioresource Technology, 2013
    Co-Authors: Zara Merali, Samuel R A Collins, Adam Elliston, Andres Kasper, Gwenaelle Le Gall, Keith W Waldron
    Abstract:

    Thermophysical pretreatment enhances the enzymatic hydrolysis of lignoCellulose. However, its impact on Cell Wall chemistry is still poorly understood. This paper reports the effects of hydrothermal pretreatment on the degradation and alkali-extractability of wheat straw Cell Wall polymers. Pretreatment resulted in loss and/or solubilization of arabinoxylans (by 53%), ferulic and diferulic acids which are important cross-linking agents accompanied by concomitant increases in Cellulose (up to 43%) and lignin (29%). The remaining water-insoluble hemiCelluloses were more readily extractable in alkali and were reduced in molecular weight indicating substantial thermochemical depolymerization. They were also associated with smaller but significant amounts of (Cellulose-derived) glucose. The alkali-insoluble residues consisted predominantly of Cellulosic glucose and lignin and contained p-coumaric acid. The depolymerization of hemiCelluloses, reduction in cinnamic acids and partial degradation of Cellulose is likely to contribute significantly to the accessibility of Cellulases during subsequent enzymolysis.

Maksudbek Yusupov - One of the best experts on this subject based on the ideXlab platform.

  • plasma induced destruction of bacterial Cell Wall Components a reactive molecular dynamics simulation
    Journal of Physical Chemistry C, 2013
    Co-Authors: Maksudbek Yusupov, Annemie Bogaerts, Stijn Huygh, Ramses Snoeckx, Adri C T Van Duin, Erik C Neyts
    Abstract:

    Nonthermal atmospheric pressure plasmas are gaining increasing attention for biomedical applications. However, very little fundamental information on the interaction mechanisms between the plasma species and biological Cells is currently available. We investigate the interaction of important plasma species, such as OH, H2O2, O, O3, as well as O2 and H2O, with bacterial peptidoglycan by means of reactive molecular dynamics simulations, aiming for a better understanding of plasma disinfection. Our results show that OH, O, O3, and H2O2 can break structurally important bonds of peptidoglycan (i.e., C–O, C–N, or C–C bonds), which consequently leads to the destruction of the bacterial Cell Wall. The mechanisms behind these breakups are, however, dependent on the impinging plasma species, and this also determines the effectiveness of the Cell Wall destruction.

  • plasma induced destruction of bacterial Cell Wall Components a reactive molecular dynamics simulation
    Journal of Physical Chemistry C, 2013
    Co-Authors: Maksudbek Yusupov, Annemie Bogaerts, Stijn Huygh, Ramses Snoeckx, Adri C T Van Duin, Erik C Neyts
    Abstract:

    Nonthermal atmospheric pressure plasmas are gaining increasing attention for biomedical applications. However, very little fundamental information on the interaction mechanisms between the plasma s...

Henry Yang - One of the best experts on this subject based on the ideXlab platform.

  • substrate utilization by endophytic bacteria paenibacillus polymyxa p2b 2r that may facilitate bacterial entrance and survival inside diverse plant hosts
    FACETS, 2017
    Co-Authors: Henry Yang, Akshit Puri, Kiran Preet Padda, Chris P. Chanway
    Abstract:

    Bacterial endophytes are thought to enter plants either through pre-existing openings in plant tissues or by creating openings by hydrolyzing major plant Cell Wall Components. A lodgepole endophyte...

  • Substrate utilization by endophytic bacteria Paenibacillus polymyxa P2b-2R that may facilitate bacterial entrance and survival inside diverse plant hosts
    Canadian Science Publishing, 2017
    Co-Authors: Henry Yang, Akshit Puri, Kiran Preet Padda, Chris P. Chanway
    Abstract:

    Bacterial endophytes are thought to enter plants either through pre-existing openings in plant tissues or by creating openings by hydrolyzing major plant Cell Wall Components. A lodgepole endophyte, Paenibacillus polymyxa P2b-2R, consistently formed endophytic colonies when inoculated in diverse plant hosts, viz., lodgepole pine, western red cedar, corn, canola, and tomato. We were interested to know, whether or not this bacterial strain possesses enzymes that can hydrolyze three major plant Cell Wall Components namely Cellulose, xylan, and pectin to facilitate entrance into the host plants. Using a BIOLOG assay, we also tested this bacterial strain’s ability to utilize carbon sources that might facilitate its entrance and hence its survival inside host plants. Paenibacillus polymyxa P2b-2R hydrolyzed sodium carboxymethylCellulose, beechwood xylan, and sodium polypectate and utilized 39 of the 95 carbon sources (41%) tested. Of the 39 carbon substrates oxidized by P2b-2R, the “carbohydrates” group represents the largest source of utilizable carbon (23 out of 39). Thus, it can be concluded that P. polymyxa P2b-2R is able to degrade major Cell Wall Components (Cellulose, xylan, and pectin) and utilize some of the available carbon substrates, possibly to gain entry and survive inside the plant and form endophytic colonies thereafter