The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Yi-wei Tang - One of the best experts on this subject based on the ideXlab platform.
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Real-time Cellular Analysis for quantitative detection of functional Clostridium difficile toxin in stool
Expert review of molecular diagnostics, 2014Co-Authors: Bin Huang, Dazhi Jin, Charles W. Stratton, Yi-wei TangAbstract:Rapid and accurate diagnosis and monitoring of Clostridium difficile infection (CDI) is critical for patient care and infection control. We will briefly review current laboratory techniques for the diagnosis of CDI and identify aspects needing improvement. We will also introduce a real-time Cellular Analysis (RTCA) assay developed for the diagnosis and monitoring of CDI using electronic impedance to assess the cell status. The RTCA assay uses impedance measurement to detect minute physiological changes in cells cultured on gold microelectrodes embedded in glass substrates in the bottom of microtiter wells. This assay has been adapted for quantitative detection of C. difficile functional toxin directly from stool specimens. Compared to conventional techniques and molecular assays, the RTCA assay provides a valuable tool for the diagnosis of CDI as well as for the assessment of clinical severity and for monitoring therapeutic efficacies.
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Real-Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool
Journal of clinical microbiology, 2014Co-Authors: Bin Huang, Dazhi Jin, J. Zhang, Janet Sun, X. Wang, Jeffrey Stiles, Mini Kamboj, N. E. Babady, Yi-wei TangAbstract:We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time Cellular Analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarrheal stool specimens, 53 (13.2%) were toxin-producing C. difficile strains by quantitative toxigenic culture (qTC); bacterial loads ranged from 3.00 × 101 to 3.69 × 106 CFU/ml. The RTCA version 2 method detected CDT in 51 samples, resulting in a sensitivity of 96.2%, a specificity of 99.7%, and positive and negative predictive values of 98.1% and 99.4%, respectively. The positive step time ranged from 1.43 to 35.85 h, with
Fang Fang - One of the best experts on this subject based on the ideXlab platform.
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Cellular Analysis and detection using surface plasmon resonance imaging
TrAC Trends in Analytical Chemistry, 2018Co-Authors: You-peng Chen, Jinsong Guo, Wei Wang, Fang FangAbstract:Abstract Surface plasmon resonance imaging (SPRi) is a label-free, non-invasion detection method that allows real-time Analysis for biomolecular interactions with high sensitivity and high throughput. Cellular Analysis and detection is significant in many research areas, including healthcare, food safety, environmental and agricultural monitoring, as well as for the application of SPRi. This review summarizes the utilization of SPRi for Cellular Analysis and detection in the last decade and illustrates the boundless potential of the SPRi technique in these fields.
Bin Huang - One of the best experts on this subject based on the ideXlab platform.
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Real-time Cellular Analysis for quantitative detection of functional Clostridium difficile toxin in stool
Expert review of molecular diagnostics, 2014Co-Authors: Bin Huang, Dazhi Jin, Charles W. Stratton, Yi-wei TangAbstract:Rapid and accurate diagnosis and monitoring of Clostridium difficile infection (CDI) is critical for patient care and infection control. We will briefly review current laboratory techniques for the diagnosis of CDI and identify aspects needing improvement. We will also introduce a real-time Cellular Analysis (RTCA) assay developed for the diagnosis and monitoring of CDI using electronic impedance to assess the cell status. The RTCA assay uses impedance measurement to detect minute physiological changes in cells cultured on gold microelectrodes embedded in glass substrates in the bottom of microtiter wells. This assay has been adapted for quantitative detection of C. difficile functional toxin directly from stool specimens. Compared to conventional techniques and molecular assays, the RTCA assay provides a valuable tool for the diagnosis of CDI as well as for the assessment of clinical severity and for monitoring therapeutic efficacies.
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Real-Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool
Journal of clinical microbiology, 2014Co-Authors: Bin Huang, Dazhi Jin, J. Zhang, Janet Sun, X. Wang, Jeffrey Stiles, Mini Kamboj, N. E. Babady, Yi-wei TangAbstract:We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time Cellular Analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarrheal stool specimens, 53 (13.2%) were toxin-producing C. difficile strains by quantitative toxigenic culture (qTC); bacterial loads ranged from 3.00 × 101 to 3.69 × 106 CFU/ml. The RTCA version 2 method detected CDT in 51 samples, resulting in a sensitivity of 96.2%, a specificity of 99.7%, and positive and negative predictive values of 98.1% and 99.4%, respectively. The positive step time ranged from 1.43 to 35.85 h, with
Hiroaki Shinohara - One of the best experts on this subject based on the ideXlab platform.
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Two-Dimensional Surface Plasmon Resonance Imaging System for Cellular Analysis.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Tanveer Ahmad Mir, Hiroaki ShinoharaAbstract:Optical biosensors based on surface plasmon resonance (SPR) phenomenon have received a great deal of attention in Cellular Analysis applications. Sensitive and high-resolution SPR imaging (SPRi) platforms are very useful for real-time monitoring and measurement of individual cell responses to various exogenous substances. In Cellular Analysis, mainstream SPR-based sensors have potential for investigations of cell responses under ambient conditions. Evaluations that account only for the average response of cell monolayers mask the understanding of precise cell-molecular interactions or intraCellular reactions at the level of individual cells. SPR/SPRi technology has attracted a great deal of attention for detecting the response of cell monolayers to various substances cultivated on the gold sensor chip. To unleash the full strength of SPRi technology in complex cell bio-systems, the applied SPR imaging system needs to be sufficiently effective to allow evaluation of a compound's potency, specificity, selectivity, toxicity, and effectiveness at the level of the individual cell. In our studies, we explore the utility of high-resolution 2D-SPR imaging for real-time monitoring of intraCellular translocation of protein kinase C (PKC), and detection of neuronal differentiation in live cells at the level of individual cells. The PC12 cell line, which is one of the most commonly used neuronal precursor cell lines for research on neuronal differentiation, was chosen as a nerve cell model. Two dimensional SPR (2D-SPR) signals/images are successfully generated. We have found that cells treated with the differentiation factor nerve growth factor (NGF) showed a remarkable enhancement of SPR response to stimulation by muscarine, a nonselective agonist of the muscarinic acetylcholine receptor.
You-peng Chen - One of the best experts on this subject based on the ideXlab platform.
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Cellular Analysis and detection using surface plasmon resonance imaging
TrAC Trends in Analytical Chemistry, 2018Co-Authors: You-peng Chen, Jinsong Guo, Wei Wang, Fang FangAbstract:Abstract Surface plasmon resonance imaging (SPRi) is a label-free, non-invasion detection method that allows real-time Analysis for biomolecular interactions with high sensitivity and high throughput. Cellular Analysis and detection is significant in many research areas, including healthcare, food safety, environmental and agricultural monitoring, as well as for the application of SPRi. This review summarizes the utilization of SPRi for Cellular Analysis and detection in the last decade and illustrates the boundless potential of the SPRi technique in these fields.