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Yoshihito Honda - One of the best experts on this subject based on the ideXlab platform.
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estrogen protects against Cellular Infiltration by reducing the expressions of e selectin and il 6 in endotoxin induced uveitis
Journal of Immunology, 1999Co-Authors: Noriko Miyamoto, Michiko Mandai, Kiyoshi Suzuma, Izumi Suzuma, Kaori Kobayashi, Yoshihito HondaAbstract:Anterior uveitis associated with Behcet’s disease and ankylosing spondylitis preferentially occurs in adult men, which may suggest the effects of sex hormones on acute anterior uveitis. Recently, estrogen receptors in the vascular endothelium have been reported to be involved in several pathological conditions. In the present study, we examined the gender differences in susceptibility to endotoxin-induced uveitis (EIU) and the effects of estrogen on anterior inflammation. EIU was induced in adult male, female, and ovariectomized female Lewis rats (200 g) by hind footpad injection of 200 μg of LPS. In EIU, Cellular Infiltration was more marked in male than in female rats, and ovariectomy increased Cellular Infiltration. Treatment with 10 μg of 17β-estradiol significantly reduced the cell number in male and ovariectomized female rats with EIU. Estrogen receptor immunoreactivity was found in the nucleus of vascular endothelium and in some stromal cells of the iris-ciliary body. Semiquantitative PCR revealed that E-selectin and IL-6 gene expressions were increased in rats following LPS injection, and an overdose of tamoxifen, an estrogen receptor antagonist, reversed the effect of 17β-estradiol on E-selectin, but not its effect on IL-6. These observations suggested that the down-modulation of these inflammatory genes by estrogen may contribute to the reduction in Cellular Infiltration in acute anterior uveitis.
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contribution of e selectin to Cellular Infiltration during endotoxin induced uveitis
Investigative Ophthalmology & Visual Science, 1998Co-Authors: Izumi Suzuma, Michiko Mandai, Shinichiro J. Tojo, Kiyoshi Suzuma, Kazuhiro Ishida, Yoshihito HondaAbstract:PURPOSE. The selectin family is a group of early-reactive adhesion molecules that plays a role in the rolling phase of leukocytes in Cellular Infiltration. It has been reported that P-selectin is expressed on vascular endothelium in the iris-ciliary body 15 minutes after lipopolysaccharide (LPS) treatment in endotoxin-induced uveitis (EIU) and may contribute to the initial phase of ocular inflammation. The objective of the present study was to identify the expression pattern of E-selectin, another member of the selectin family, and to investigate the role of E-selectin during the course of EIU. METHODS. Endotoxin-induced uveitis was induced in male Lewis rats by a footpad injection of 200 /Lig LPS. The time-dependent expressions of E-selectin in EIU in the iris- ciliary body and the retina were studied by immunohistochemistry using wholemounts and paraffin-embedded sections and by monitoring the level of E-selectin mRNA expression. A monoclonal antibody to E-selectin and a control antibody were each injected intravenously to evaluate the effects of E-selectin inhibition on ocular inflammation at the time of maximum uveitis. In the anterior uvea, the effect was evaluated by the number of infiltrated cells and by the protein concentration in the aqueous humor 24 hours after LPS treatment; in the retina, the myeloperoxidase (MPO) activity was measured 48 hours after LPS treatment. The effect of the combined injection of anti-P-selectin and anti-E-selectin antibodies was also studied. It was then determined whether the delayed inhibition of E-selectin (6, 12, or 24 hours after LPS injection) could contribute to the early resolution of the uveitis. RESULTS. E-selectin immunoreactivity was observed on the vessel walls of the iris and retina 7 hours after LPS treatment in wholemounts and paraffin-embedded sections and remained positive for 24 hours after LPS treatment. The expression of E-selectin messenger RNA gene peaked at 6 hours and again at 18 hours after LPS treatment in the iris- ciliary body and retina. The expression returned to the basal level 24 hours after LPS treatment in the iris- ciliary body and 48 hours after LPS treatment in the retina. The selective inhibition of E-selectin significantly blocked die Cellular Infiltration into the aqueous humor (P < 0.005) but had a milder effect on the protein concentration in the aqueous humor (P = 0.0536). The inhibition of E-selectin and P-selectin almost abrogated Cellular Infiltration (P < 0.001). Myeloperoxidase activity in the retina 48 hours after LPS treatment was again significantly decreased by the inhibition of E-selectin alone and by the inhibition of E-selectin and P-selectin (P < 0.0001). A single injection of anti-E-selectin antibody 6, 12, or 24 hours after LPS injection effectively blocked Cellular Infiltration in the aqueous humor 24 and 48 hours after LPS treatment. CONCLUSIONS. In EIU, E-selectin may be expressed on the vascular endothelium and remain after the period of expression of P-selectin and until approximately the time of maximum uveitis. The present results suggest that, in contrast to the role of P-selectin as an initiator of Cellular Infiltration, E-selectin may contribute to continuing Cellular Infiltration into the inflammatory site during inflammation, and its inhibition may contribute to the early resolution of the uveitis. (Invest Ophthalmol Vis Set. 1998;39:1620 -1630)
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Role of P-selectin in endotoxin-induced uveitis.
Investigative Ophthalmology & Visual Science, 1997Co-Authors: Kiyoshi Suzuma, Michiko Mandai, Jun-ichi Kogishi, Shinichiro J. Tojo, Yoshihito Honda, Nagahisa YoshimuraAbstract:Purpose. P-selectin is one of the early-reactive adhesion molecules that play a part in the rolling phase of leukocytes in Cellular Infiltration. The study objective was to determine whether P-selectin is involved in the development of endotoxin-induced uveitis (EIU). Methods. Endotoxin-induced uveitis was initiated in male Lewis rats by injecting 200 μg lipopolysaccharide (LPS) into the foot pad. Expression of P-selectin in the iris-ciliary body was studied by immunohistochemistry using wholemounts and paraffin embedded sections of iris-ciliary body prepared at various time intervals. A monoclonal antibody (mAh) against P-selectin and a ligand for P-selectin, sialyl Lewis X oligosaccharide (SLeX-OS), was intravenously injected to evaluate the effects of selectin inhibition. The effect of treatment was evaluated by the number of infiltrated cells and protein concentration in the aqueous humor at 24 hours after LPS treatment. Results. P-selectin immunoreactivities were observed in the vessels of the iris in whole iris-ciliary body mounts and on the surface of the microvascular endothelium in paraffin-embedded sections. Activity was most prominent at 15 minutes and at 5 to 7 hours after LPS treatment and was moderate from 1 to 4 hours after treatment. The selective inhibition of P-selectin significantly blocked the Cellular Infiltration into aqueous humor, but this Infiltration was even more effectively inhibited by SLeX-OS. Protein concentration in the aqeous humor was not inhibited by selectin as much as was Cellular Infiltration. Conclusions. In the early phase of EIU, P-selectin may be expressed on the vascular endothelium in the iris in a biphasic pattern that modulates the rolling phase of leukocytes. The expression of this molecule may be essential for succeeding processes of Cellular Infiltration and may determine the subsequent states of ocular inflammation.
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Role of P-selectin in endotoxin-induced uveitis.
Investigative Ophthalmology & Visual Science, 1997Co-Authors: Kiyoshi Suzuma, Michiko Mandai, Jun-ichi Kogishi, Shinichiro J. Tojo, Yoshihito Honda, Nagahisa YoshimuraAbstract:PURPOSE: P-selectin is one of the early-reactive adhesion molecules that play a part in the rolling phase of leukocytes in Cellular Infiltration. The study objective was to determine whether P-selectin is involved in the development of endotoxin-induced uveitis (EIU). METHODS: Endotoxin-induced uveitis was initiated in male Lewis rats by injecting 200 micrograms lipopolysaccharide (LPS) into the foot pad. Expression of P-selectin in the iris-ciliary body was studied by immunohistochemistry using wholemounts and paraffin embedded sections of iris-ciliary body prepared at various time intervals. A monoclonal antibody (mAb) against P-selectin and a ligand for P-selectin, sialyl Lewis X oligosaccharide (SLeX-OS), was intravenously injected to evaluate the effects of selectin inhibition. The effect of treatment was evaluated by the number of infiltrated cells and protein concentration in the aqueous humor at 24 hours after LPS treatment. RESULTS: P-selectin immunoreactivities were observed in the vessels of the iris in whole iris-ciliary body mounts and on the surface of the microvascular endothelium in paraffin-embedded sections. Activity was most prominent at 15 minutes and at 5 to 7 hours after LPS treatment and was moderate from 1 to 4 hours after treatment. The selective inhibition of P-selectin significantly blocked the Cellular Infiltration into aqueous humor, but this Infiltration was even more effectively inhibited by SLeX-OS. Protein concentration in the aqueous humor was not inhibited by selectin as much as was Cellular Infiltration. CONCLUSIONS: In the early phase of EIU, P-selectin may be expressed on the vascular endothelium in the iris in a biphasic pattern that modulates the rolling phase of leukocytes. The expression of this molecule may be essential for succeeding processes of Cellular Infiltration and may determine the subsequent states of ocular inflammation.
J. Lalitha - One of the best experts on this subject based on the ideXlab platform.
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Cellular Infiltration at skin lesions and draining lymph nodes of sheep infested with adult Hyalomma anatolicum anatolicum ticks.
Parasitology, 2020Co-Authors: D. K. V. Boppana, S. K. Wikel, M. B. Manohar, J. LalithaAbstract:Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tick Hyalomma anatolicum anatolicum were studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal Infiltration of CD8+ and gammadelta+ T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1+ Cellular Infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4:CD8 T cells and MHC-II:CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of alphabeta:gammadelta T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8+, gammadelta+ T and CD1+ Cellular Infiltration compared to control lymph nodes. CD4+ T cells were decreased. There were no significant changes in CD45R+ Cellular Infiltration either at skin lesions or regional lymph nodes.
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Cellular Infiltration at skin lesions and draining lymph nodes of sheep infested with adult hyalomma anatolicum anatolicum ticks
Parasitology, 2005Co-Authors: D. K. V. Boppana, S. K. Wikel, M. B. Manohar, J. LalithaAbstract:Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tick Hyalomma anatolicum anatolicum were studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal Infiltration of CD8 + and γδ + T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1 + Cellular Infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4[ratio ]CD8 T cells and MHC-II[ratio ]CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of αβ[ratio ]γδ T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8 + , γδ + T and CD1 + Cellular Infiltration compared to control lymph nodes. CD4 + T cells were decreased. There were no significant changes in CD45R + Cellular Infiltration either at skin lesions or regional lymph nodes.
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Cellular Infiltration at skin lesions and draining lymph nodes of sheep infested with adultHyalomma anatolicum anatolicumticks
Parasitology, 2005Co-Authors: D. K. V. Boppana, S. K. Wikel, M. B. Manohar, J. LalithaAbstract:Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tickHyalomma anatolicum anatolicumwere studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal Infiltration of CD8+and γδ+T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1+Cellular Infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4[ratio ]CD8 T cells and MHC-II[ratio ]CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of αβ[ratio ]γδ T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8+, γδ+T and CD1+Cellular Infiltration compared to control lymph nodes. CD4+T cells were decreased. There were no significant changes in CD45R+Cellular Infiltration either at skin lesions or regional lymph nodes.
Yanzhong Zhang - One of the best experts on this subject based on the ideXlab platform.
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fabrication of large pores in electrospun nanofibrous scaffolds for Cellular Infiltration a review
Tissue Engineering Part B-reviews, 2012Co-Authors: Shaoping Zhong, Yanzhong ZhangAbstract:In the past decade, considerable effort has been made to construct biomimetic scaffolds from electrospun nanofibers for engineering different tissues. However, one of the major concerns with electr...
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Fabrication of Large Pores in Electrospun Nanofibrous Scaffolds for Cellular Infiltration: A Review
Tissue Engineering Part B: Reviews, 2012Co-Authors: Shaoping Zhong, Yanzhong Zhang, Chwee Teck LimAbstract:In the past decade, considerable effort has been made to construct biomimetic scaffolds from electrospun nanofibers for engineering different tissues. However, one of the major concerns with electrospun nanofibrous scaffolds is that the densely arranged architecture of fibers and small pores or voids between fibers hinder efficient Cellular Infiltration or prevent three dimensional (3D) Cellular integration with host tissue in vivo after implantation. To overcome this problem, many concepts or strategies applicable during the electrospinning or post-electrospinning procedures have been proposed to enlarge pore size of electrospun scaffolds. This article addresses the issues of pore geometry and Cellular Infiltration of electrospun scaffolds, and first reviews the fabrication solutions/approaches applied to achieve larger micropores in electrospun mats. The evidence and potential for fostering Cellular Infiltration using these improved porous scaffolds are then discussed. Finally, it is hoped that this will enable us to better exploit viable technologies or develop new ones for constructing ideal nanofibrous architecture for fulfilling specific tissue engineering needs.
Shaoping Zhong - One of the best experts on this subject based on the ideXlab platform.
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fabrication of large pores in electrospun nanofibrous scaffolds for Cellular Infiltration a review
Tissue Engineering Part B-reviews, 2012Co-Authors: Shaoping Zhong, Yanzhong ZhangAbstract:In the past decade, considerable effort has been made to construct biomimetic scaffolds from electrospun nanofibers for engineering different tissues. However, one of the major concerns with electr...
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Fabrication of Large Pores in Electrospun Nanofibrous Scaffolds for Cellular Infiltration: A Review
Tissue Engineering Part B: Reviews, 2012Co-Authors: Shaoping Zhong, Yanzhong Zhang, Chwee Teck LimAbstract:In the past decade, considerable effort has been made to construct biomimetic scaffolds from electrospun nanofibers for engineering different tissues. However, one of the major concerns with electrospun nanofibrous scaffolds is that the densely arranged architecture of fibers and small pores or voids between fibers hinder efficient Cellular Infiltration or prevent three dimensional (3D) Cellular integration with host tissue in vivo after implantation. To overcome this problem, many concepts or strategies applicable during the electrospinning or post-electrospinning procedures have been proposed to enlarge pore size of electrospun scaffolds. This article addresses the issues of pore geometry and Cellular Infiltration of electrospun scaffolds, and first reviews the fabrication solutions/approaches applied to achieve larger micropores in electrospun mats. The evidence and potential for fostering Cellular Infiltration using these improved porous scaffolds are then discussed. Finally, it is hoped that this will enable us to better exploit viable technologies or develop new ones for constructing ideal nanofibrous architecture for fulfilling specific tissue engineering needs.
D. K. V. Boppana - One of the best experts on this subject based on the ideXlab platform.
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Cellular Infiltration at skin lesions and draining lymph nodes of sheep infested with adult Hyalomma anatolicum anatolicum ticks.
Parasitology, 2020Co-Authors: D. K. V. Boppana, S. K. Wikel, M. B. Manohar, J. LalithaAbstract:Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tick Hyalomma anatolicum anatolicum were studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal Infiltration of CD8+ and gammadelta+ T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1+ Cellular Infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4:CD8 T cells and MHC-II:CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of alphabeta:gammadelta T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8+, gammadelta+ T and CD1+ Cellular Infiltration compared to control lymph nodes. CD4+ T cells were decreased. There were no significant changes in CD45R+ Cellular Infiltration either at skin lesions or regional lymph nodes.
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Cellular Infiltration at skin lesions and draining lymph nodes of sheep infested with adult hyalomma anatolicum anatolicum ticks
Parasitology, 2005Co-Authors: D. K. V. Boppana, S. K. Wikel, M. B. Manohar, J. LalithaAbstract:Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tick Hyalomma anatolicum anatolicum were studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal Infiltration of CD8 + and γδ + T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1 + Cellular Infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4[ratio ]CD8 T cells and MHC-II[ratio ]CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of αβ[ratio ]γδ T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8 + , γδ + T and CD1 + Cellular Infiltration compared to control lymph nodes. CD4 + T cells were decreased. There were no significant changes in CD45R + Cellular Infiltration either at skin lesions or regional lymph nodes.
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Cellular Infiltration at skin lesions and draining lymph nodes of sheep infested with adultHyalomma anatolicum anatolicumticks
Parasitology, 2005Co-Authors: D. K. V. Boppana, S. K. Wikel, M. B. Manohar, J. LalithaAbstract:Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tickHyalomma anatolicum anatolicumwere studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal Infiltration of CD8+and γδ+T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1+Cellular Infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4[ratio ]CD8 T cells and MHC-II[ratio ]CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of αβ[ratio ]γδ T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8+, γδ+T and CD1+Cellular Infiltration compared to control lymph nodes. CD4+T cells were decreased. There were no significant changes in CD45R+Cellular Infiltration either at skin lesions or regional lymph nodes.