The Experts below are selected from a list of 4260 Experts worldwide ranked by ideXlab platform
Glenn D Prestwich - One of the best experts on this subject based on the ideXlab platform.
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rapid biofabrication of tubular tissue constructs by Centrifugal Casting in a decellularized natural scaffold with laser machined micropores
Journal of Materials Science: Materials in Medicine, 2009Co-Authors: V. Kasyanov, Jason P Hodde, Michael C Hiles, Carol A Eisenberg, Leonard M Eisenberg, Luis Fernandez E De Castro, Iveta Ozolanta, Modra Murovska, Robert A Draughn, Glenn D PrestwichAbstract:Centrifugal Casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by Centrifugal Casting and increase hydrogel retention, without compromising the biomechanical properties of the scaffold. Micropores with diameters of 50, 100, and 200 μm were machined at different linear densities in decellularized small intestine submucosa (SIS) planar sheets and tubular SIS scaffolds using an argon laser. The ultimate stress and ultimate strain values for SIS sheets with laser-machined micropores with diameter 50 μm and distance between holes as low as 714 μm were not significantly different from unmachined control SIS specimens. Centrifugal Casting of GFP-labeled cells suspended in an in situ cross-linkable hyaluronan-based hydrogel resulted in scaffold recellularization with a high density of viable cells inside the laser-machined micropores. Perfusion tests demonstrated the retention of the cells encapsulated within the HA hydrogel in the microholes. Thus, an SIS scaffold with appropriately sized microholes can be loaded with hydrogel encapsulated cells by Centrifugal Casting to give a mechanically robust construct that retains the cell-seeded hydrogel, permitting rapid biofabrication of tubular tissue construct in a “bioreactor-free” fashion.
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting
Expert Opinion on Biological Therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they d...
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting.
Expert opinion on biological therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they discuss the potential future for clinical applications.
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fabrication of tubular tissue constructs by Centrifugal Casting of cells suspended in an in situ crosslinkable hyaluronan gelatin hydrogel
Biomaterials, 2005Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Carol A Eisenberg, Leonard M Eisenberg, Steve Gonda, Thomas C Trusk, Glenn D PrestwichAbstract:Achieving the optimal cell density and desired cell distribution in scaffolds is a major goal of cell seeding technologies in tissue engineering. In order to reach this goal, a novel Centrifugal Casting technology was developed using in situ crosslinkable hyaluronan-based (HA) synthetic extracellular matrix (sECM). Living cells were suspended in a viscous solution of thiol-modified HA and thiol-modified gelatin, a polyethyleneglycol diacrylate crosslinker was added, and a hydrogel was formed during rotation. The tubular tissue constructs consisting of a densely packed cell layer were fabricated with the rotation device operating at 2000 rpm for 10 min. The majority of cells suspended in the HA mixture before rotation were located inside the layer after Centrifugal Casting. Cells survived the effect of the Centrifugal forces experienced under the rotational regime employed. The volume cell density (65.6%) approached the maximal possible volume density based on theoretical sphere packing models. Thus, Centrifugal Casting allows the fabrication of tubular constructs with the desired redistribution, composition and thickness of cell layers that makes the maximum efficient use of available cells. Centrifugal Casting in this sECM would enable rapid fabrication of tissue-engineered vascular grafts, as well as other tubular and planar tissue-engineered constructs.
V. Kasyanov - One of the best experts on this subject based on the ideXlab platform.
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rapid biofabrication of tubular tissue constructs by Centrifugal Casting in a decellularized natural scaffold with laser machined micropores
Journal of Materials Science: Materials in Medicine, 2009Co-Authors: V. Kasyanov, Jason P Hodde, Michael C Hiles, Carol A Eisenberg, Leonard M Eisenberg, Luis Fernandez E De Castro, Iveta Ozolanta, Modra Murovska, Robert A Draughn, Glenn D PrestwichAbstract:Centrifugal Casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by Centrifugal Casting and increase hydrogel retention, without compromising the biomechanical properties of the scaffold. Micropores with diameters of 50, 100, and 200 μm were machined at different linear densities in decellularized small intestine submucosa (SIS) planar sheets and tubular SIS scaffolds using an argon laser. The ultimate stress and ultimate strain values for SIS sheets with laser-machined micropores with diameter 50 μm and distance between holes as low as 714 μm were not significantly different from unmachined control SIS specimens. Centrifugal Casting of GFP-labeled cells suspended in an in situ cross-linkable hyaluronan-based hydrogel resulted in scaffold recellularization with a high density of viable cells inside the laser-machined micropores. Perfusion tests demonstrated the retention of the cells encapsulated within the HA hydrogel in the microholes. Thus, an SIS scaffold with appropriately sized microholes can be loaded with hydrogel encapsulated cells by Centrifugal Casting to give a mechanically robust construct that retains the cell-seeded hydrogel, permitting rapid biofabrication of tubular tissue construct in a “bioreactor-free” fashion.
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting
Expert Opinion on Biological Therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they d...
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting.
Expert opinion on biological therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they discuss the potential future for clinical applications.
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fabrication of tubular tissue constructs by Centrifugal Casting of cells suspended in an in situ crosslinkable hyaluronan gelatin hydrogel
Biomaterials, 2005Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Carol A Eisenberg, Leonard M Eisenberg, Steve Gonda, Thomas C Trusk, Glenn D PrestwichAbstract:Achieving the optimal cell density and desired cell distribution in scaffolds is a major goal of cell seeding technologies in tissue engineering. In order to reach this goal, a novel Centrifugal Casting technology was developed using in situ crosslinkable hyaluronan-based (HA) synthetic extracellular matrix (sECM). Living cells were suspended in a viscous solution of thiol-modified HA and thiol-modified gelatin, a polyethyleneglycol diacrylate crosslinker was added, and a hydrogel was formed during rotation. The tubular tissue constructs consisting of a densely packed cell layer were fabricated with the rotation device operating at 2000 rpm for 10 min. The majority of cells suspended in the HA mixture before rotation were located inside the layer after Centrifugal Casting. Cells survived the effect of the Centrifugal forces experienced under the rotational regime employed. The volume cell density (65.6%) approached the maximal possible volume density based on theoretical sphere packing models. Thus, Centrifugal Casting allows the fabrication of tubular constructs with the desired redistribution, composition and thickness of cell layers that makes the maximum efficient use of available cells. Centrifugal Casting in this sECM would enable rapid fabrication of tissue-engineered vascular grafts, as well as other tubular and planar tissue-engineered constructs.
Vadim Mironov - One of the best experts on this subject based on the ideXlab platform.
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting
Expert Opinion on Biological Therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they d...
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting.
Expert opinion on biological therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they discuss the potential future for clinical applications.
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fabrication of tubular tissue constructs by Centrifugal Casting of cells suspended in an in situ crosslinkable hyaluronan gelatin hydrogel
Biomaterials, 2005Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Carol A Eisenberg, Leonard M Eisenberg, Steve Gonda, Thomas C Trusk, Glenn D PrestwichAbstract:Achieving the optimal cell density and desired cell distribution in scaffolds is a major goal of cell seeding technologies in tissue engineering. In order to reach this goal, a novel Centrifugal Casting technology was developed using in situ crosslinkable hyaluronan-based (HA) synthetic extracellular matrix (sECM). Living cells were suspended in a viscous solution of thiol-modified HA and thiol-modified gelatin, a polyethyleneglycol diacrylate crosslinker was added, and a hydrogel was formed during rotation. The tubular tissue constructs consisting of a densely packed cell layer were fabricated with the rotation device operating at 2000 rpm for 10 min. The majority of cells suspended in the HA mixture before rotation were located inside the layer after Centrifugal Casting. Cells survived the effect of the Centrifugal forces experienced under the rotational regime employed. The volume cell density (65.6%) approached the maximal possible volume density based on theoretical sphere packing models. Thus, Centrifugal Casting allows the fabrication of tubular constructs with the desired redistribution, composition and thickness of cell layers that makes the maximum efficient use of available cells. Centrifugal Casting in this sECM would enable rapid fabrication of tissue-engineered vascular grafts, as well as other tubular and planar tissue-engineered constructs.
Roger R. Markwald - One of the best experts on this subject based on the ideXlab platform.
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting
Expert Opinion on Biological Therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they d...
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Bioreactor-free tissue engineering: directed tissue assembly by Centrifugal Casting.
Expert opinion on biological therapy, 2008Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Glenn D PrestwichAbstract:Casting is a process by which a material is introduced into a mold while it is liquid, allowed to solidify in a predefined shape inside the mold, and then removed to give a fabricated object, part or casing. Centrifugal Casting could be defined as a process of molding using Centrifugal forces. Although the Centrifugal Casting technology has a long history in metal manufacturing and in the plastics industry, only recently has this technology attracted the attention of tissue engineers. Initially, centrifugation was used to optimize cell seeding on a solid scaffold. More recently, Centrifugal Casting has been used to create tubular scaffolds and both tubular and flat multilayered, living tissue constructs. These newer applications were enabled by a new class of biocompatible in situ crosslinkable hydrogels that mimic the extracellular matrix. Herein the authors summarize the state of the art of Centrifugal Casting technology in tissue engineering, they outline associated technological challenges, and they discuss the potential future for clinical applications.
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fabrication of tubular tissue constructs by Centrifugal Casting of cells suspended in an in situ crosslinkable hyaluronan gelatin hydrogel
Biomaterials, 2005Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Carol A Eisenberg, Leonard M Eisenberg, Steve Gonda, Thomas C Trusk, Glenn D PrestwichAbstract:Achieving the optimal cell density and desired cell distribution in scaffolds is a major goal of cell seeding technologies in tissue engineering. In order to reach this goal, a novel Centrifugal Casting technology was developed using in situ crosslinkable hyaluronan-based (HA) synthetic extracellular matrix (sECM). Living cells were suspended in a viscous solution of thiol-modified HA and thiol-modified gelatin, a polyethyleneglycol diacrylate crosslinker was added, and a hydrogel was formed during rotation. The tubular tissue constructs consisting of a densely packed cell layer were fabricated with the rotation device operating at 2000 rpm for 10 min. The majority of cells suspended in the HA mixture before rotation were located inside the layer after Centrifugal Casting. Cells survived the effect of the Centrifugal forces experienced under the rotational regime employed. The volume cell density (65.6%) approached the maximal possible volume density based on theoretical sphere packing models. Thus, Centrifugal Casting allows the fabrication of tubular constructs with the desired redistribution, composition and thickness of cell layers that makes the maximum efficient use of available cells. Centrifugal Casting in this sECM would enable rapid fabrication of tissue-engineered vascular grafts, as well as other tubular and planar tissue-engineered constructs.
Carol A Eisenberg - One of the best experts on this subject based on the ideXlab platform.
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rapid biofabrication of tubular tissue constructs by Centrifugal Casting in a decellularized natural scaffold with laser machined micropores
Journal of Materials Science: Materials in Medicine, 2009Co-Authors: V. Kasyanov, Jason P Hodde, Michael C Hiles, Carol A Eisenberg, Leonard M Eisenberg, Luis Fernandez E De Castro, Iveta Ozolanta, Modra Murovska, Robert A Draughn, Glenn D PrestwichAbstract:Centrifugal Casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by Centrifugal Casting and increase hydrogel retention, without compromising the biomechanical properties of the scaffold. Micropores with diameters of 50, 100, and 200 μm were machined at different linear densities in decellularized small intestine submucosa (SIS) planar sheets and tubular SIS scaffolds using an argon laser. The ultimate stress and ultimate strain values for SIS sheets with laser-machined micropores with diameter 50 μm and distance between holes as low as 714 μm were not significantly different from unmachined control SIS specimens. Centrifugal Casting of GFP-labeled cells suspended in an in situ cross-linkable hyaluronan-based hydrogel resulted in scaffold recellularization with a high density of viable cells inside the laser-machined micropores. Perfusion tests demonstrated the retention of the cells encapsulated within the HA hydrogel in the microholes. Thus, an SIS scaffold with appropriately sized microholes can be loaded with hydrogel encapsulated cells by Centrifugal Casting to give a mechanically robust construct that retains the cell-seeded hydrogel, permitting rapid biofabrication of tubular tissue construct in a “bioreactor-free” fashion.
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fabrication of tubular tissue constructs by Centrifugal Casting of cells suspended in an in situ crosslinkable hyaluronan gelatin hydrogel
Biomaterials, 2005Co-Authors: Vadim Mironov, Roger R. Markwald, V. Kasyanov, Carol A Eisenberg, Leonard M Eisenberg, Steve Gonda, Thomas C Trusk, Glenn D PrestwichAbstract:Achieving the optimal cell density and desired cell distribution in scaffolds is a major goal of cell seeding technologies in tissue engineering. In order to reach this goal, a novel Centrifugal Casting technology was developed using in situ crosslinkable hyaluronan-based (HA) synthetic extracellular matrix (sECM). Living cells were suspended in a viscous solution of thiol-modified HA and thiol-modified gelatin, a polyethyleneglycol diacrylate crosslinker was added, and a hydrogel was formed during rotation. The tubular tissue constructs consisting of a densely packed cell layer were fabricated with the rotation device operating at 2000 rpm for 10 min. The majority of cells suspended in the HA mixture before rotation were located inside the layer after Centrifugal Casting. Cells survived the effect of the Centrifugal forces experienced under the rotational regime employed. The volume cell density (65.6%) approached the maximal possible volume density based on theoretical sphere packing models. Thus, Centrifugal Casting allows the fabrication of tubular constructs with the desired redistribution, composition and thickness of cell layers that makes the maximum efficient use of available cells. Centrifugal Casting in this sECM would enable rapid fabrication of tissue-engineered vascular grafts, as well as other tubular and planar tissue-engineered constructs.