The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Stephen F. Badylak - One of the best experts on this subject based on the ideXlab platform.
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Identification of extractable growth factors from small intestinal Submucosa.
Journal of cellular biochemistry, 1997Co-Authors: Sherry L. Voytik-harbin, Andrew O. Brightman, Meredith R. Kraine, Beverly Z. Waisner, Stephen F. BadylakAbstract:When implanted as a biomaterial for tissue replacement, selected Submucosal layers of porcine small intestine induce site-specific tissue remodeling. Small intestinal Submucosa (SIS), as isolated, is primarily an acellular extracellular matrix mater~al. In an attempt to discover the components of small intestinal Submucosa which are able to induce this tissue remodeling, the material was extracted and extracts were tested for the ability to stimulate Swiss 3T3 fibroblasts to synthesize DNA and proliferate. Each oi the iour different extracts of small intestinal Submucosa had measurablecell-stimulating activity when analyzed in both a whole cell proliferation assay (alamarBlue dye reduction) and a DNA synthesis assay (('HI-thymidine incorporation). Proteins extracted from SIS with 2 M urea induced activity profiles in the two assays which were very similar to the activity profiles of basic fibroblast growth factor (FGF-2) in the assays. As well, the changes in cell morphology in response to the extracted proteins mimicked the changes induced by FGF-2. Neutralization experiments with specific antibodies to this growth factor confirmed the presence of FGF-2 and indicated that it was responsible for 60% oi the fibroblast-stimulating activity of the urea extract oi small intestinal Submucosa. Western blot analvsis with a monoclonal antibody specific for FGF-2 detected a reactive doublet at approximately 19 kDa and further confirmed the presence oi FGF-2. Cell stimulat~ng activity oi proteins extracted irom SIS with 4 M guanidine was neutralized by an antibody specific ior transforming growth factor P CTCFB). Changes in the morphology oi the fibroblasts exposed to this extract were nearly identical to changes induced by TGFB. Although no reactive protein band was detected at 25 kDa in nonreduced western blot analysis, several bands were reactive at higher molecular weight. The identity of this TGFf3-related component of small intestinal Submucosa is unknown. Identification of FGF-2 and TGFp-related activities in SIS, two growth tactors known to significantly affect critical processes oi tissue development and difierentiation, provides the opportunity to further elucidate the mechanisms by which this extracellu- larmatrix biomaterial modulates wound healing and tissueremodeling. J. Cell. Biochem. 67:478491,1997. Q 1997wik+~. ~nc
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characterization of small intestinal Submucosa regenerated canine detrusor assessment of reinnervation in vitro compliance and contractility
The Journal of Urology, 1996Co-Authors: Bradley P. Kropp, Barry D. Sawyer, Harlon E. Shannon, Marian K. Rippy, Stephen F. BadylakAbstract:AbstractPurpose: We characterized small intestinal Submucosa regenerated canine bladder.Materials and Methods: We subjected 15-month small intestinal Submucosa regenerated canine bladder strips to in vitro muscle bath compliance, contractility testing and immunohistochemical staining.Results: Compliance studies demonstrated no significant difference between small intestinal Submucosa regenerated and control bladders, which were 30-fold more compliant than native small intestinal Submucosal graft material. Contractility studies demonstrated contractile responses and innervation similar to those of normal canine bladder. Afferent nerves were demonstrated through immunohistochemical techniques.Conclusions: These characteristics further support the regenerative capacity of small intestinal Submucosa and its potential use as a bladder augmentation material.
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Characterization of Small Intestinal Submucosa Regenerated Canine Detrusor: Assessment of Reinnervation, in Vitro Compliance and Contractility
The Journal of urology, 1996Co-Authors: Bradley P. Kropp, Barry D. Sawyer, Harlon E. Shannon, Marian K. Rippy, Stephen F. Badylak, Mark C. Adams, Michael A. Keating, Richard C. Rink, Karl B. ThorAbstract:We characterized small intestinal Submucosa regenerated canine bladder. We subjected 15-month small intestinal Submucosa regenerated canine bladder strips to in vitro muscle bath compliance, contractility testing and immunohistochemical staining. Compliance studies demonstrated no significant difference between small intestinal Submucosa regenerated and control bladders, which were 30-fold more compliant than native small intestinal Submucosal graft material. Contractility studies demonstrated contractile responses and innervation similar to those of normal canine bladder. Afferent nerves were demonstrated through immunohistochemical techniques. These characteristics further support the regenerative capacity of small intestinal Submucosa and its potential use as a bladder augmentation material.
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biocompatibility of small intestinal Submucosa in urinary tract as augmentation cystoplasty graft and injectable suspension
Journal of Endourology, 1994Co-Authors: Peter M Knapp, Stephen F. Badylak, James E Lingeman, Yoram I Siegel, Robert J DemeterAbstract:We explored the biocompatibility of fluidized canine small-intestinal Submucosa (SIS) for periureteral injection and as a patch graft for bladder augmentation in pigs. Gross evaluation 8 weeks after Submucosal injection showed persistence of the nodule. Histologic examination showed thickened Submucosa with spindle cells embedded in poorly organized fibrous material. There was no evidence of inflammatory reaction or granuloma formation. Subserosal nodules likewise persisted and demonstrated capillary ingrowth. Grafts of SIS became epithelialized within 3 weeks with maintenance of bladder capacity. Ingrowth of capillaries and smooth muscle could be seen in later specimens. Although further studies with longer follow-up are needed, SIS appears to be a promising graft material in the urinary tract.
Hyun Jik Lee - One of the best experts on this subject based on the ideXlab platform.
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A proposal for the management guideline of superficial esophageal squamous cell carcinoma: After endoscopic resection.
Journal of Clinical Oncology, 2016Co-Authors: Yong Chan Lee, Jun Chul Park, Sung Kwan Shin, Sang Kil Lee, Hyun Jik LeeAbstract:145 Background: We tried to analyze and deduce a treatment guideline after endoscopic resection of superficial esophageal squamous cell carcinoma in a retrospective analysis. Methods: Medical records of 37 patients who had been treated by endoscopic resection during a 6-year period in single institution were retrospectively analyzed. Results: Mean tumor size was 11.5±5.5 mm (range 3-31). Thirty-one lesions (83.8%) were treated by endoscopic Submucosal dissection and 6 lesions by endoscopic mucosal resection (16.2%). En bloc resection rate and complete resection rate was 91.9% and 81.8%, respectively. The tumor invasion depth was diagnosed as epithelium: 5(13.5%), lamina propria mucosa: 12 (32.4%), muscularis mucosa: 10 (27.0%), Submucosa: 10 (27.0%) respectively. The lymphovascular invasion was 3 lesions (8.1%). The rates of adverse events were 13.5% including 3 cases (8.1%) of perforation. Ten patients of the invasion depth as muscularis mucosa and Submucosa received additional treatments including 6 pat...
Oscar W. Cummings - One of the best experts on this subject based on the ideXlab platform.
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Obliterative Muscularization of the Small Bowel Submucosa in Crohn Disease
Archives of Pathology & Laboratory Medicine, 2001Co-Authors: George K. Koukoulis, John D. Henley, Oscar W. CummingsAbstract:Abstract Context.—The pathology of small bowel obstruction in Crohn disease has not been studied extensively. Stricture formation has been attributed mainly to fibrosis, although muscularization of the Submucosa has been discussed previously. Objective.—To identify additional pathologic changes in Crohn disease that could be involved in the formation of strictures. Design.—We reviewed 50 ileal resections from patients with Crohn disease. The histopathologic slides were reviewed initially without knowledge of the macroscopic or clinical findings. We identified an unusual muscular proliferation that we refer to as obliterative muscularization of the Submucosa, defined as a thick and continuous muscle layer from the mucosal base to the muscularis propria that is at least 1 cm in length. Subsequently, histopathologic findings were correlated with macroscopic and clinical findings. Results.—Obliterative muscularization of the Submucosa was present in 14 specimens, and in 11 of these 14 it was topographically restricted to strictures. Submucosal fibrosis was observed in sections from adjacent regions. Obliterative muscularization of the Submucosa, including thick-walled vessels and hyperplastic nerves but not prominent scarring, was more common in specimens with strictures; the difference was statistically significant (P < .001). Conclusions.—Obliterative muscularization of the Submucosa may be pathogenetically involved in the formation of strictures either directly by causing a sustained spasm, or indirectly by minimizing the vasoprotective role of the Submucosa, impairing repair and enhancing scarring.
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Obliterative Muscularization of the Small Bowel Submucosa in Crohn Disease A Possible Mechanism of Small Bowel Obstruction
Archives of pathology & laboratory medicine, 2001Co-Authors: George K. Koukoulis, John D. Henley, Oscar W. CummingsAbstract:The pathology of small bowel obstruction in Crohn disease has not been studied extensively. Stricture formation has been attributed mainly to fibrosis, although muscularization of the Submucosa has been discussed previously. To identify additional pathologic changes in Crohn disease that could be involved in the formation of strictures. We reviewed 50 ileal resections from patients with Crohn disease. The histopathologic slides were reviewed initially without knowledge of the macroscopic or clinical findings. We identified an unusual muscular proliferation that we refer to as obliterative muscularization of the Submucosa, defined as a thick and continuous muscle layer from the mucosal base to the muscularis propria that is at least 1 cm in length. Subsequently, histopathologic findings were correlated with macroscopic and clinical findings. Obliterative muscularization of the Submucosa was present in 14 specimens, and in 11 of these 14 it was topographically restricted to strictures. Submucosal fibrosis was observed in sections from adjacent regions. Obliterative muscularization of the Submucosa, including thick-walled vessels and hyperplastic nerves but not prominent scarring, was more common in specimens with strictures; the difference was statistically significant (P <.001). Obliterative muscularization of the Submucosa may be pathogenetically involved in the formation of strictures either directly by causing a sustained spasm, or indirectly by minimizing the vasoprotective role of the Submucosa, impairing repair and enhancing scarring.
Yong Chan Lee - One of the best experts on this subject based on the ideXlab platform.
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A proposal for the management guideline of superficial esophageal squamous cell carcinoma: After endoscopic resection.
Journal of Clinical Oncology, 2016Co-Authors: Yong Chan Lee, Jun Chul Park, Sung Kwan Shin, Sang Kil Lee, Hyun Jik LeeAbstract:145 Background: We tried to analyze and deduce a treatment guideline after endoscopic resection of superficial esophageal squamous cell carcinoma in a retrospective analysis. Methods: Medical records of 37 patients who had been treated by endoscopic resection during a 6-year period in single institution were retrospectively analyzed. Results: Mean tumor size was 11.5±5.5 mm (range 3-31). Thirty-one lesions (83.8%) were treated by endoscopic Submucosal dissection and 6 lesions by endoscopic mucosal resection (16.2%). En bloc resection rate and complete resection rate was 91.9% and 81.8%, respectively. The tumor invasion depth was diagnosed as epithelium: 5(13.5%), lamina propria mucosa: 12 (32.4%), muscularis mucosa: 10 (27.0%), Submucosa: 10 (27.0%) respectively. The lymphovascular invasion was 3 lesions (8.1%). The rates of adverse events were 13.5% including 3 cases (8.1%) of perforation. Ten patients of the invasion depth as muscularis mucosa and Submucosa received additional treatments including 6 pat...
Tomohiko Okawa - One of the best experts on this subject based on the ideXlab platform.
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Treatment of superficial esophageal cancer by external radiation therapy alone: results of a multi-institutional experience
International Journal of Radiation Oncology Biology Physics, 2000Co-Authors: Kenji Nemoto, Yasuo Matsumoto, Michitaka Yamakawa, Seiya Jo, Naoki Kokubo, Yasumasa Nishimura, Shogo Yamada, Masahiko Oguchi, Tomohiko OkawaAbstract:Abstract Purpose: To assess the effectiveness and toxicity of external radiation therapy for superficial esophageal cancer. Methods and Materials: During the period from March 1979 to November 1996, 78 patients with superficial esophageal cancer received radiation therapy without intracavitary irradiation at nine radiotherapy institutions in Japan. All patients had histologically-proven squamous cell carcinoma. Endoscopic ultrasonography was performed in 34 patients to discriminate mucosal from Submucosal cancer. Most of the patients had received radiation therapy using conventional fractionation at an average dose of 65.5 Gy. Results: The survival rates at 1, 2, and 5 years were 88%, 73%, and 45%, respectively. The local control rates at 1, 2, and 5 years were 85%, 79%, and 66%, respectively. Although the difference was not significant, the survival rate of cancer patients with a tumor invading the Submucosa was lower than that of the other patients. In 6 mucosal cancer patients, local recurrence was observed in 1 patient with extensive cancer. Regional lymph node recurrence and distant failure were not observed in mucosal cancer patients, while in 28 Submucosal cancer patients, the 5-year survival rate and relapse free rate were only 49% and 43%, respectively. Univariate and multivariate analysis identified age as the only significant prognostic factor. Severe late injury, such as esophageal ulcer, perforation, and bleeding, was not observed. Conclusion: External radiation therapy is effective for mucosal cancer. However, further investigation is needed to establish a better standard treatment protocol for Submucosal cancer.
