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Gabriel Annick - One of the best experts on this subject based on the ideXlab platform.

  • Etude morphologique et histiologique du cerveau chez le mouton
    'Wiley', 2011
    Co-Authors: Salouci Moustafa, Engelen Virginie, Jacqmot Olivier, Antoine Nadine, Kirschvink Nathalie, Gabriel Annick
    Abstract:

    peer reviewedaudience: researcher, professional, student, popularization, otherIntroduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and Cerebellum Cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the Cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus

  • Etude morphologique et histiologique du cerveau chez le mouton
    'Wiley', 2010
    Co-Authors: Salouci Moustafa, Engelen Virginie, Jacqmot Olivier, Antoine Nadine, Kirschvink Nathalie, Gabriel Annick
    Abstract:

    Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and Cerebellum Cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the Cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus.Peer reviewe

Zimatkin S. M. - One of the best experts on this subject based on the ideXlab platform.

  • Bile loss damages the cerebellar Purkinje cells in rats
    ГрГМУ, 2018
    Co-Authors: Emelyanchik S., Zimatkin S. M.
    Abstract:

    Cerebellum, Purkinje cells, structure, cytochemistry, loss of bileAim: The aim of the study was the estimation of the structural and metabolic changes in cerebellar Purkinje cells of rats during the complete outside bile abduction. Material and methods: Experiments were performed on male Wistar rats weighing 200-250 g. In 26 rats the fistula of the common bile duct was made and all the bile secreted by the liver was collected through a plastic tube into the outside glass bile container. 20 animals of the control group underwent sham operation (no removal of bile). On the 1th, 3th, and 5th days after the operation the animals of the control and experimental groups were decapitated and Cerebellum Cortex samples were collected for histology and cytochemistry. Results: The loss of bile induced the gradual increase in structural and metabolic abnormalities of Purkinje cells resulting in severe, irreversible disturbances and even death of some of them. The gradual decrease in Purkinje cells size, the loss of their sphericity and elongation, followed by inhibition of succinate-, NADH-, glucose-6-phosphate-dehydrogenases and the activation of lactate dehydrogenase and the marker enzyme of lysosomes acid phosphatase were revealed. All the changes reflect the ratio of the processes of damage and adaptation in the affected neurons in bile absence in the body. Conclusion: Total loss of bile (on the 1st, 3rd, and 5th days) induces the gradual increase in structural and metabolic disturbances in Cerebellum Purkinje cells and death of some of them

  • BILE LOSS DAMAGES THE CEREBELLAR PURKINJE CELLS IN RATS
    Grodno State Medical University, 2018
    Co-Authors: Emelyanchik S.v., Zimatkin S. M.
    Abstract:

    Aim: The aim of the study was the estimation of the structural and metabolic changes in cerebellar Purkinje cells of rats during the complete outside bile abduction. Material and methods: Experiments were performed on male Wistar rats weighing 200-250 g. In 26 rats the fistula of the common bile duct was made and all the bile secreted by the liver was collected through a plastic tube into the outside glass bile container. 20 animals of the control group underwent sham operation (no removal of bile). On the 1th, 3th, and 5th days after the operation the animals of the control and experimental groups were decapitated and Cerebellum Cortex samples were collected for histology and cytochemistry. Results: The loss of bile induced the gradual increase in structural and metabolic abnormalities of Purkinje cells resulting in severe, irreversible disturbances and even death of some of them. The gradual decrease in Purkinje cells size, the loss of their sphericity and elongation, followed by inhibition of succinate-, NADH-, glucose-6-phosphate-dehydrogenases and the activation of lactate dehydrogenase and the marker enzyme of lysosomes acid phosphatase were revealed. All the changes reflect the ratio of the processes of damage and adaptation in the affected neurons in bile absence in the body. Conclusion: Total loss of bile (on the 1st, 3rd, and 5th days) induces the gradual increase in structural and metabolic disturbances in Cerebellum Purkinje cells and death of some of them

Salouci Moustafa - One of the best experts on this subject based on the ideXlab platform.

  • Etude morphologique et histiologique du cerveau chez le mouton
    'Wiley', 2011
    Co-Authors: Salouci Moustafa, Engelen Virginie, Jacqmot Olivier, Antoine Nadine, Kirschvink Nathalie, Gabriel Annick
    Abstract:

    peer reviewedaudience: researcher, professional, student, popularization, otherIntroduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and Cerebellum Cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the Cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus

  • Etude morphologique et histiologique du cerveau chez le mouton
    'Wiley', 2010
    Co-Authors: Salouci Moustafa, Engelen Virginie, Jacqmot Olivier, Antoine Nadine, Kirschvink Nathalie, Gabriel Annick
    Abstract:

    Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and Cerebellum Cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the Cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus.Peer reviewe

Kimberly B Kegelgleason - One of the best experts on this subject based on the ideXlab platform.

  • mass spectrometry analysis of wild type and knock in q140 q140 huntington s disease mouse brains reveals changes in glycerophospholipids including alterations in phosphatidic acid and lyso phosphatidic acid
    Journal of Huntington's disease, 2015
    Co-Authors: Petr Vodicka, Adelaide Tousley, Karin M Green, Ellen Sapp, Maria Iuliano, Ghazaleh Sadrivakili, Scott A Shaffer, Neil Aronin, Marian Difiglia, Kimberly B Kegelgleason
    Abstract:

    Background Huntington's disease (HD) is a neurodegenerative disease caused by a CAG expansion in the HD gene, which encodes the protein Huntingtin. Huntingtin associates with membranes and can interact directly with glycerophospholipids in membranes. Objective We analyzed glycerophospholipid profiles from brains of 11 month old wild-type (WT) and Q140/Q140 HD knock-in mice to assess potential changes in glycerophospholipid metabolism. Methods Polar lipids from Cerebellum, Cortex, and striatum were extracted and analyzed by liquid chromatography and negative ion electrospray tandem mass spectrometry analysis (LC-MS/MS). Gene products involved in polar lipid metabolism were studied using western blotting, immuno-electron microscopy and qPCR. Results Significant changes in numerous species of glycerophosphate (phosphatidic acid, PA) were found in striatum, Cerebellum and Cortex from Q140/Q140 HD mice compared to WT mice at 11 months. Changes in specific species could also be detected for other glycerophospholipids. Increases in species of lyso-PA (LPA) were measured in striatum of Q140/Q140 HD mice compared to WT. Protein levels for c-terminal binding protein 1 (CtBP1), a regulator of PA biosynthesis, were reduced in striatal synaptosomes from HD mice compared to wild-type at 6 and 12 months. Immunoreactivity for CtBP1 was detected on membranes of synaptic vesicles in striatal axon terminals in the globus pallidus. Conclusions These novel results identify a potential site of molecular pathology caused by mutant Huntingtin that may impart early changes in HD.

Liliana Rivera-espinosa - One of the best experts on this subject based on the ideXlab platform.

  • Biochemical and molecular modulation of CCl4-induced peripheral and central damage by Tilia americana var. mexicana
    Elsevier, 2017
    Co-Authors: Elvia Coballase-urrutia, Noemí Cárdenas-rodríguez, María Carolina González-garcía, Eithan Núñez-ramírez, Esaú Floriano-sánchez, María Eva González-trujano, Berenice Fernández-rojas, José Pedraza-chaverrí, Hortencia Montesinos-correa, Liliana Rivera-espinosa
    Abstract:

    Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p.) obtained from the leaves of Tilia americana var. mexicana on CCl4-induced liver and brain damage in the rat. Protection was observed in the liver and brain (Cerebellum, Cortex and cerebral hemispheres) by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA) using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl4-treated rats. The T. americana var. mexicana leaf extracts provided significant protection against CCl4-induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-globulin (γ-GLOB), serum albumin (ALB), total bilirubin (BB), creatinine (CREA) and creatine kinase (CK), relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous Tilia extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of T. americana var. mexicana that confer its neuro- and hepatoprotective effects