The Experts below are selected from a list of 8034 Experts worldwide ranked by ideXlab platform
Jingjing Duan - One of the best experts on this subject based on the ideXlab platform.
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dietary Cerebroside from sea cucumber stichopus japonicus absorption and effects on skin barrier and cecal short chain fatty acids
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Kazuhiko Aida, Jingjing Duan, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial eff...
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Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids
2016Co-Authors: Jingjing Duan, Kazuhiko Aida, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host
Tatsuya Sugawara - One of the best experts on this subject based on the ideXlab platform.
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dietary Cerebroside from sea cucumber stichopus japonicus absorption and effects on skin barrier and cecal short chain fatty acids
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Kazuhiko Aida, Jingjing Duan, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial eff...
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Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids
2016Co-Authors: Jingjing Duan, Kazuhiko Aida, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host
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isolation and anti fatty liver activity of a novel Cerebroside from the sea cucumber acaudina molpadioides
Bioscience Biotechnology and Biochemistry, 2011Co-Authors: Xu Jie, Tatsuya Sugawara, Yuming Wang, Tingyu Feng, Bei Zhang, Changhu XueAbstract:Cerebrosides are a kind of important bioactive substance in sea cucumber. A novel Cerebroside, AMC-2, was purified from the less-polar lipid fraction of the sea cucumber Acaudina molpadioides by repeated column chromatography. The major structure of AMC-2 was analyzed by gas chromatography-mass spectra. The amide-linked fatty acid unit was confirmed to be four saturated and monounsaturated α-hydroxy fatty acids, the long-chain base was dihydroxy sphingoid base with one double bond, and the glycosyl group was glucose. We also investigated the anti-fatty liver activity of AMC-2 in rats with fatty liver induced by orotic acid. AMC-2 significantly reduced hepatic triglyceride (TG) and total cholesterol (TC) levels at a diet supplement of 0.03% and 0.006%. The indexes of stearoyl-CoA desaturase (SCD) activity and mRNA expression were significantly decreased by AMC-2. This indicates that AMC-2 ameliorated nonalcoholic fatty liver disease (NAFLD) through suppression of SCD activity and impaired the biosynthesis of monounsaturated fatty acids in the livers of the rats.
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Prevention of melanin formation by yeast Cerebroside in B16 mouse melanoma cells.
Journal of oleo science, 2007Co-Authors: Mikio Kinoshita, Naofumi Hori, Kazuhiko Aida, Tatsuya Sugawara, Masao OhnishiAbstract:The effects of plant and yeast Cerebrosides on melanin formation were examined in B16 mouse melanoma cells. Addition of yeast Cerebroside significantly reduced melanin content to the same level as that of arbutin in control cells, although there was no suppression by plant Cerebrosides and bovine brain Cerebroside up-regulated melanin formation. None of the bovine brain Cerebrosides examined had any effect on tyrosinase activity, but yeast Cerebroside reduced the contents of tyrosinase . The results of the present study clearly showed that melanin formation is regulated by several different Cerebrosides via tyrosinase. In addition, the findings presented here suggest that Cerebrosides containing a 9-methyl type sphingoid base, such as yeast Cerebroside, may be useful as skincare products for suppressing melanin formation.
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method for quantitative determination of Cerebroside in plant ceramide foodstuffs by high performance liquid chromatography with evaporative light scattering detection
Journal of Oleo Science, 2002Co-Authors: Minoru Kashima, Tatsuya Sugawara, Kiyotaka Nakagawa, Teruo Miyazawa, Chiaki Murakami, Rumiko Miyashita, Jisaburo Ono, F S Deschamps, P ChaminadeAbstract:Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining Cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic Cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of Cerebroside, the equation for which was y=axb (x: Cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of Cerebroside. Determination on three foodstuffs each containing Cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining Cerebroside content in “Plant ceramide” food samples.
Takashi Hirata - One of the best experts on this subject based on the ideXlab platform.
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dietary Cerebroside from sea cucumber stichopus japonicus absorption and effects on skin barrier and cecal short chain fatty acids
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Kazuhiko Aida, Jingjing Duan, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial eff...
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Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids
2016Co-Authors: Jingjing Duan, Kazuhiko Aida, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host
Kazuhiko Aida - One of the best experts on this subject based on the ideXlab platform.
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dietary Cerebroside from sea cucumber stichopus japonicus absorption and effects on skin barrier and cecal short chain fatty acids
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Kazuhiko Aida, Jingjing Duan, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial eff...
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Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids
2016Co-Authors: Jingjing Duan, Kazuhiko Aida, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of Cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of Cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of Cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber Cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host
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Prevention of melanin formation by yeast Cerebroside in B16 mouse melanoma cells.
Journal of oleo science, 2007Co-Authors: Mikio Kinoshita, Naofumi Hori, Kazuhiko Aida, Tatsuya Sugawara, Masao OhnishiAbstract:The effects of plant and yeast Cerebrosides on melanin formation were examined in B16 mouse melanoma cells. Addition of yeast Cerebroside significantly reduced melanin content to the same level as that of arbutin in control cells, although there was no suppression by plant Cerebrosides and bovine brain Cerebroside up-regulated melanin formation. None of the bovine brain Cerebrosides examined had any effect on tyrosinase activity, but yeast Cerebroside reduced the contents of tyrosinase . The results of the present study clearly showed that melanin formation is regulated by several different Cerebrosides via tyrosinase. In addition, the findings presented here suggest that Cerebrosides containing a 9-methyl type sphingoid base, such as yeast Cerebroside, may be useful as skincare products for suppressing melanin formation.
Masao Ohnishi - One of the best experts on this subject based on the ideXlab platform.
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Prevention of melanin formation by yeast Cerebroside in B16 mouse melanoma cells.
Journal of oleo science, 2007Co-Authors: Mikio Kinoshita, Naofumi Hori, Kazuhiko Aida, Tatsuya Sugawara, Masao OhnishiAbstract:The effects of plant and yeast Cerebrosides on melanin formation were examined in B16 mouse melanoma cells. Addition of yeast Cerebroside significantly reduced melanin content to the same level as that of arbutin in control cells, although there was no suppression by plant Cerebrosides and bovine brain Cerebroside up-regulated melanin formation. None of the bovine brain Cerebrosides examined had any effect on tyrosinase activity, but yeast Cerebroside reduced the contents of tyrosinase . The results of the present study clearly showed that melanin formation is regulated by several different Cerebrosides via tyrosinase. In addition, the findings presented here suggest that Cerebrosides containing a 9-methyl type sphingoid base, such as yeast Cerebroside, may be useful as skincare products for suppressing melanin formation.
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effects of growth temperature on Cerebroside content and chemical composition in kluyveromyces lactis
Journal of Oleo Science, 2004Co-Authors: Mikio Tanji, Mikio Kinoshita, Hiroshi Yada, Masaki Yamane, Yukiko Kakuta, Hidemasa Motoshima, Yuji Oda, Masao OhnishiAbstract:For assessment of the physiological significance of 9-methyl-4-trans,8-trans-sphingadienine (9-methyl base) in Cerebroside-accumulating yeast, determination was made of the content and chemical compositions of Cerebrosides in Kluyveromyces lactis and the results were compared for growth temperatures at 15°C, 25°C and 35°C. Compared with the culture prepared at the optimal growth temperature (25°C), greater time was required to reach the stationary phase of cells grown at 15°C. A reduction in cell weight was noted at 35°C. Cerebroside content in cells cultured at 15°C was significantly less than at 25°C or 35°C. Also, the ratio of Cerebroside to phosphorus in the total lipid fraction at 15°C was less than at 25°C. In the 15°C culture, the 9-methyl base, the most predominant fungal Cerebroside sphingoid base, increased beyond that in the 25°C preparation, though was essentially the same as at 35°C. K. lactis is thus shown to modulate not only the content but sphingoid base composition of Cerebrosides in response to temperature. Fatty acid compositions in all Cerebrosides examined were the same. These and the results from a previous study (J. Oleo Sci., Vol. 51, 741-747, 2002) provide basis for assuming 9-methyl branched chain in yeast Cerebroside molecules to be essential for maintaining sufficient membrane fluidity for a low temperature environment.
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Content and chemical compositions of Cerebrosides in lactose-assimilating yeasts.
Bioscience biotechnology and biochemistry, 2004Co-Authors: Mikio Tanji, Mikio Kinoshita, Hidemasa Motoshima, Yuji Oda, Kanae Namimatsu, Masao OhnishiAbstract:Cerebrosides were found in ten lactose-assimilating yeasts. Representative component ceramide moieties of Cerebrosides from nine of these yeasts contained 9-methyl-4-trans, 8-trans-sphingadienine, and 2-hydroxy fatty acid with carbon chain lengths of 16 or 18. The major ceramide moieties in Brettanomyces anomalus, however, differed from those in other yeasts, and were predominately moieties containing 2-hydroxymyristic acid. Thus we found that various Cerebroside molecular species are present in yeasts.
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Properties and Physiological Effects of Plant Cerebroside Species as Functional Lipids
Advanced Research on Plant Lipids, 2003Co-Authors: K. Aida, Mikio Kinoshita, N. Takakuwa, T. Sugawara, H. Imai, J. Ono, Masao OhnishiAbstract:The properties and physiological effects of plant Cerebrosides were investigated. The major sphingoid bases of Cerebrosides from soybean, maize, and rice bran were trans-4, cis or trans-8-sphingadienine. However, the major sphingoid bases in wheat and rye grains were 8-sphingenine mainly with the cis-configuration (more than 60%). The phase transition temperature of plant Cerebrosides measured by differential scan calorimetric method was significant lower than that of bovine brain Cerebroside. Plant Cerebroside-derived compounds such as sphingoid bases induced apoptosis in human colon cancer cell lines. This result suggested that dietary plant Cerebrosides have a potent physiological function similar to that of animal sphingolipids.
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Distribution of Cerebroside in Genus Saccharomyces and Its Closely Related Yeasts, and Cloning of Cerebroside Metabolism-Related Genes
Advanced Research on Plant Lipids, 2003Co-Authors: Naoya Takakuwa, Mikio Kinoshita, Yuji Oda, K. Yamane, Masao OhnishiAbstract:As sphingolipids, Cerebroside (glucosylceramide) as well as free ceramide and inositolphosphate-containing ceramide, are widely distributed in fungi and plants. However, there has been no evidence of Cerebroside or unsaturated sphingoid bases found in Saccharomyces yeasts. When thirty-one strains accepted in the genus Saccharomyces and its closely related yeasts were analyzed for sphingolipids, Cerebrosides were found in Saccharomyces kluyveri, Zygosaccharomyces cidri, Z. fermentati, Kluyveromyces lactis, K. thermotolerans, and K. waltii. The Cerebrosides of S. kluyveri and K. lactis included 9-methyl-trans-4, trans-8-sphingadienine and its putative metabolic intermediates; 4-unsaturated and 4,8-diunsaturated sphingoid bases. We further cloned genes encoding glucosylceramide synthase and Δ8-sphingolipid desaturase from S. kluyveri and K. lactis.