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Osamu Shibata - One of the best experts on this subject based on the ideXlab platform.
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langmur monolayers of cerebroside with different head groups originated from sea cucumber binary systems with dipalmitoylphosphatidylcholine dppc
Colloids and Surfaces B: Biointerfaces, 2009Co-Authors: Yuriko Ikeda, Masanori Inagaki, Ryuichi Higuchi, Tomofumi Miyamoto, Koji Yamada, Osamu ShibataAbstract:Surface properties (Langmuir monolayer) of two different Cerebrosides which are extracted from the sea cucumber (Bohadschia argus) were investigated. A main difference in chemical structure of cerebroside between BAC-2a and BAC-4 is their head groups (glucose and galactose, respectively). Furthermore, miscibility and interaction between dipalmitoylphosphatidylcholine (DPPC) and Cerebrosides (BAC-2a and BAC-4) in the monolayer have been systematically examined. The surface pressure (pi)-area (A), the surface potential (Delta V)-A, and the dipole moment (mu(perpendicular))-A isotherms for monolayers of DPPC, Cerebrosides, and their binary combinations have been measured using the Wilhelmy method and the ionizing electrode method. BAC-4 forms a stable liquid-expanded (LE) monolayer, whereas BAC-2a has a first-order phase transition from the LE phase to the liquid-condensed (LC) state on 0.15 M NaCl at 298.2 K. The fundamental properties for each cerebroside monolayer were elucidated in terms of the surface dipole moment based on the three-layer model [R.J. Demchak, T. Fort Jr., J. Colloid Interface Sci. 46 (1974) 191-202] for both Cerebrosides and the apparent molar quantity change (Delta s(gamma), Delta h(gamma), and Delta u(gamma)) for BAC-2a. In addition, their miscibility with DPPC was examined by the variation of the molecular areas and the surface potentials as a function of cerebroside mole fractions, the additivity rule. The miscibility was also confirmed by constructing the two-dimensional phase diagrams. The phase diagrams for the both binary systems were of negative azeotropic type. That is, the two-component DPPC/BAC-2a and DPPC/BAC-4 monolayers are miscible. Furthermore, the Joos equation for the analysis of the collapse pressure of binary monolayers allowed calculation of the interaction parameter and the interaction energy between the DPPC and cerebroside monolayers. The miscibility in the monolayer state was also confirmed by the morphological observation with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM).
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Biologically Active Glycosides from Asteroidea, 41. Isolation and Structure Determination of GlucoCerebrosides from the Starfish Linckia laevigata
Chemical & Pharmaceutical Bulletin, 2005Co-Authors: Tomoki Maruta, Masanori Inagaki, Osamu Shibata, Takeshi Saito, Ryuichi HiguchiAbstract:A new glucocerebroside, linckiacerebroside A (1) and a known glucocerebroside S-2a-3 (2), have been isolated from the cerebroside molecular species obtained from the less polar fraction of the CHCl3/MeOH extract of the starfish Linckia laevigata, together with three pseudo homogeneous glucocerebroside, 3, 4, and 5. The structures of these Cerebrosides were determined on the basis of chemical and spectroscopic evidence.
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Langmuir monolayers of cerebroside originated from Linckia laevigata : Binary systems of Cerebrosides and phospholipid
Colloids and Surfaces B: Biointerfaces, 2005Co-Authors: Tomoki Maruta, Masanori Inagaki, Ryuichi Higuchi, Kazuki Hoda, Osamu ShibataAbstract:Abstract The surface pressure (π)–area (A), the surface potential (ΔV)–A and the dipole moment (μ⊥)–A isotherms were obtained for six Cerebrosides of LLC-2, LLC-2-1, LLC-2-8, LLC-2-10, LLC-2-12, and LLC-2-15, which were isolated from Linckia laevigata, and two-component monolayers of two different Cerebrosides (LLC-2 and LLC-2-8) with phospholipid of dipalmitoylphosphatidylcholine (DPPC) on a subphase of 0.15 M sodium chloride solution as a function of cerebroside compositions in the two-component systems by employing the Wilhelmy method, the ionizing electrode method, and the fluorescence microscopy. The new finding was that LLC-2 showed a stable and liquid expanded type film. Four of them (LLC-2-8, -10, -12, and -15) had the phase transition from the liquid-expanded (LE) to the liquid-condensed (LC) states at 298.2 K. The apparent molar quantity changes (Δsγ, Δhγ, and Δuγ) on their phase transition on 0.15 M at 298.2 K were calculated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the cerebroside molar fraction (Xcerebroside), using the additivity rule. From the A–Xcerebroside and ΔVm–Xphospholipid plots, a partial molecular surface area (PMA) and an apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible systems. Judging from the two-dimensional phase diagrams, these were found to be one type, a positive azeotropic type; all the Cerebrosides were miscible with DPPC. Furthermore, assuming a regular surface mixture, the Joos equation for the analysis of the collapse pressure of two-component monolayers allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the Cerebrosides and DPPC. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
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Cerebroside Langmuir monolayers originated from the echinoderms
Colloids and surfaces. B Biointerfaces, 2005Co-Authors: Hiromichi Nakahara, Masanori Inagaki, Ryuichi Higuchi, Kazufumi Nakamura, Shohei Nakamura, Mariko Aso, Osamu ShibataAbstract:The surface pressure (pi)-area (A), the surface potential (DeltaV)-A and the dipole moment (mu( perpendicular))-A isotherms were obtained for two-component monolayers of two different Cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (DeltaV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (mu( perpendicular)) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (X(phospholipid)), using the additivity rule. From the A-X(phospholipid) and DeltaV(m)-X(phospholipid) plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of Cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of Cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (xi) and the interaction energy (-Delta epsilon) between the Cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
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Cerebroside Langmuir monolayers originated from the echinoderms: II. Binary systems of Cerebrosides and steroids
Colloids and surfaces. B Biointerfaces, 2005Co-Authors: Hiromichi Nakahara, Masanori Inagaki, Ryuichi Higuchi, Kazufumi Nakamura, Shohei Nakamura, Mariko Aso, Osamu ShibataAbstract:Abstract The surface pressure (π)–area (A), the surface potential (ΔV)–A and the dipole moment (μ⊥)–A isotherms were obtained for two-component monolayers of two different Cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (ΔV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort [J. Colloid Interf. Sci. 46 (1974) 191–202]. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (μ⊥) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (Xphospholipid), using the additivity rule. From the A–Xphospholipid and ΔVm–Xphospholipid plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of Cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of Cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the Cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
Tatsuya Sugawara - One of the best experts on this subject based on the ideXlab platform.
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dietary cerebroside from sea cucumber stichopus japonicus absorption and effects on skin barrier and cecal short chain fatty acids
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Kazuhiko Aida, Jingjing Duan, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial eff...
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Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids
2016Co-Authors: Jingjing Duan, Kazuhiko Aida, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host
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high throughput analysis of Cerebrosides from the sea cucumber pearsonothria graeffei by liquid chromatography quadrupole time of flight mass spectrometry
Journal of Oleo Science, 2015Co-Authors: Zicai Jia, Tatsuya Sugawara, Peixu Cong, Yuming Wang, Changhu XueAbstract:Liquid chromatography-mass spectrometry was one of the most powerful methods for identification and detection of chemical structures of lipids. In this study, the Cerebrosides molecular species from the sea cucumber Pearsonothria graeffei (P. graeffei) were high throughput identified by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). Cerebrosides were separated and purified by solid-phase extraction with chloroform-methanol solution. Precursor ion scan spectra and product ion scan spectra were obtained through auto MS/MS analysis in the positive scan. Cerebroside molecules were selected according to the neutral loss fragments of 180 Da, and then the structures were identified according to pairs of specific products of sphingoid bases and their precursor ions. Eighty-nine Cerebrosides molecular species were identified, large amounts of d17:1-C22:0 h, t17:0-C24:1h, d17:1-C24:1h, d17:1-C23:0 h, d17:1-C22:0 and d17:1-C23:0 were present which have hardly found in mammal. There were 13 classes of long-chain base (LCB), and the ratio of phytosphingosines and sphingosines was roughly 1:9, in which two of the most common LCBs were d17:1 and d18:1. The carbon numbers of fatty acids (FAs) were mainly 18~24, while 24 carbon fatty acids were predominant. The ratio of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) was about 2:3, and the percentage of hydroxy fatty acid (HFA) was over 60%. The ratio of non-hydroxylated fatty acid (NFA)/HFA was also approximately 2:3. LC-Q-TOF-MS analysis should be useful for the structure determination of diverse Cerebrosides molecular species. Meanwhile, this method provided a basis for structure-activity relationship studies and functional food development of the sea cucumber P. graeffei as well.
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Prevention of melanin formation by yeast cerebroside in B16 mouse melanoma cells.
Journal of oleo science, 2007Co-Authors: Mikio Kinoshita, Naofumi Hori, Kazuhiko Aida, Tatsuya Sugawara, Masao OhnishiAbstract:The effects of plant and yeast Cerebrosides on melanin formation were examined in B16 mouse melanoma cells. Addition of yeast cerebroside significantly reduced melanin content to the same level as that of arbutin in control cells, although there was no suppression by plant Cerebrosides and bovine brain cerebroside up-regulated melanin formation. None of the bovine brain Cerebrosides examined had any effect on tyrosinase activity, but yeast cerebroside reduced the contents of tyrosinase . The results of the present study clearly showed that melanin formation is regulated by several different Cerebrosides via tyrosinase. In addition, the findings presented here suggest that Cerebrosides containing a 9-methyl type sphingoid base, such as yeast cerebroside, may be useful as skincare products for suppressing melanin formation.
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prevention of aberrant crypt foci formation by dietary maize and yeast Cerebrosides in 1 2 dimethylhydrazine treated mice
Journal of Oleo Science, 2005Co-Authors: Mikio Kinoshita, Kazuhiko Aida, Tatsuya Sugawara, Mikio Tanji, Masahiko Tamura, Jisaburo OnoAbstract:For clarification of physiological effects of dietary maize and yeast sphingolipids on colon cancer in vivo, we investigated the effects of dietary maize and yeast Cerebrosides on 1,2-dimethylhydrazine (DMH) -induced aberrant crypt foci (ACF) formation in mouse large intestine. After 10 weeks of maize and yeast cerebroside feeding and DMH administration, DMH-induced ACF formation was significantly suppressed in all large intestines of these mice. When lipids in the feces of mice fed maize and yeast Cerebrosides were analyzed, maize and yeast Cerebrosides decomposed by intestinal enzyme and/or microflora were observed. The present study demonstrated that DMH-induced ACF formation could be inhibited in mouse large intestine by maize and yeast Cerebrosides. Daily ingestion of these compounds may serve to suppress colon carcinoma in humans.
Jingjing Duan - One of the best experts on this subject based on the ideXlab platform.
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dietary cerebroside from sea cucumber stichopus japonicus absorption and effects on skin barrier and cecal short chain fatty acids
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Kazuhiko Aida, Jingjing Duan, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial eff...
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Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids
2016Co-Authors: Jingjing Duan, Kazuhiko Aida, Marina Ishida, Tsuyoshi Tsuduki, Jin Zhang, Yuki Manabe, Takashi Hirata, Tatsuya SugawaraAbstract:Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing Cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors’ knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host
Ryuichi Higuchi - One of the best experts on this subject based on the ideXlab platform.
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langmur monolayers of cerebroside with different head groups originated from sea cucumber binary systems with dipalmitoylphosphatidylcholine dppc
Colloids and Surfaces B: Biointerfaces, 2009Co-Authors: Yuriko Ikeda, Masanori Inagaki, Ryuichi Higuchi, Tomofumi Miyamoto, Koji Yamada, Osamu ShibataAbstract:Surface properties (Langmuir monolayer) of two different Cerebrosides which are extracted from the sea cucumber (Bohadschia argus) were investigated. A main difference in chemical structure of cerebroside between BAC-2a and BAC-4 is their head groups (glucose and galactose, respectively). Furthermore, miscibility and interaction between dipalmitoylphosphatidylcholine (DPPC) and Cerebrosides (BAC-2a and BAC-4) in the monolayer have been systematically examined. The surface pressure (pi)-area (A), the surface potential (Delta V)-A, and the dipole moment (mu(perpendicular))-A isotherms for monolayers of DPPC, Cerebrosides, and their binary combinations have been measured using the Wilhelmy method and the ionizing electrode method. BAC-4 forms a stable liquid-expanded (LE) monolayer, whereas BAC-2a has a first-order phase transition from the LE phase to the liquid-condensed (LC) state on 0.15 M NaCl at 298.2 K. The fundamental properties for each cerebroside monolayer were elucidated in terms of the surface dipole moment based on the three-layer model [R.J. Demchak, T. Fort Jr., J. Colloid Interface Sci. 46 (1974) 191-202] for both Cerebrosides and the apparent molar quantity change (Delta s(gamma), Delta h(gamma), and Delta u(gamma)) for BAC-2a. In addition, their miscibility with DPPC was examined by the variation of the molecular areas and the surface potentials as a function of cerebroside mole fractions, the additivity rule. The miscibility was also confirmed by constructing the two-dimensional phase diagrams. The phase diagrams for the both binary systems were of negative azeotropic type. That is, the two-component DPPC/BAC-2a and DPPC/BAC-4 monolayers are miscible. Furthermore, the Joos equation for the analysis of the collapse pressure of binary monolayers allowed calculation of the interaction parameter and the interaction energy between the DPPC and cerebroside monolayers. The miscibility in the monolayer state was also confirmed by the morphological observation with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM).
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Biologically Active Glycosides from Asteroidea, 41. Isolation and Structure Determination of GlucoCerebrosides from the Starfish Linckia laevigata
Chemical & Pharmaceutical Bulletin, 2005Co-Authors: Tomoki Maruta, Masanori Inagaki, Osamu Shibata, Takeshi Saito, Ryuichi HiguchiAbstract:A new glucocerebroside, linckiacerebroside A (1) and a known glucocerebroside S-2a-3 (2), have been isolated from the cerebroside molecular species obtained from the less polar fraction of the CHCl3/MeOH extract of the starfish Linckia laevigata, together with three pseudo homogeneous glucocerebroside, 3, 4, and 5. The structures of these Cerebrosides were determined on the basis of chemical and spectroscopic evidence.
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Langmuir monolayers of cerebroside originated from Linckia laevigata : Binary systems of Cerebrosides and phospholipid
Colloids and Surfaces B: Biointerfaces, 2005Co-Authors: Tomoki Maruta, Masanori Inagaki, Ryuichi Higuchi, Kazuki Hoda, Osamu ShibataAbstract:Abstract The surface pressure (π)–area (A), the surface potential (ΔV)–A and the dipole moment (μ⊥)–A isotherms were obtained for six Cerebrosides of LLC-2, LLC-2-1, LLC-2-8, LLC-2-10, LLC-2-12, and LLC-2-15, which were isolated from Linckia laevigata, and two-component monolayers of two different Cerebrosides (LLC-2 and LLC-2-8) with phospholipid of dipalmitoylphosphatidylcholine (DPPC) on a subphase of 0.15 M sodium chloride solution as a function of cerebroside compositions in the two-component systems by employing the Wilhelmy method, the ionizing electrode method, and the fluorescence microscopy. The new finding was that LLC-2 showed a stable and liquid expanded type film. Four of them (LLC-2-8, -10, -12, and -15) had the phase transition from the liquid-expanded (LE) to the liquid-condensed (LC) states at 298.2 K. The apparent molar quantity changes (Δsγ, Δhγ, and Δuγ) on their phase transition on 0.15 M at 298.2 K were calculated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the cerebroside molar fraction (Xcerebroside), using the additivity rule. From the A–Xcerebroside and ΔVm–Xphospholipid plots, a partial molecular surface area (PMA) and an apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible systems. Judging from the two-dimensional phase diagrams, these were found to be one type, a positive azeotropic type; all the Cerebrosides were miscible with DPPC. Furthermore, assuming a regular surface mixture, the Joos equation for the analysis of the collapse pressure of two-component monolayers allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the Cerebrosides and DPPC. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
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Cerebroside Langmuir monolayers originated from the echinoderms
Colloids and surfaces. B Biointerfaces, 2005Co-Authors: Hiromichi Nakahara, Masanori Inagaki, Ryuichi Higuchi, Kazufumi Nakamura, Shohei Nakamura, Mariko Aso, Osamu ShibataAbstract:The surface pressure (pi)-area (A), the surface potential (DeltaV)-A and the dipole moment (mu( perpendicular))-A isotherms were obtained for two-component monolayers of two different Cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (DeltaV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (mu( perpendicular)) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (X(phospholipid)), using the additivity rule. From the A-X(phospholipid) and DeltaV(m)-X(phospholipid) plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of Cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of Cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (xi) and the interaction energy (-Delta epsilon) between the Cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
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Cerebroside Langmuir monolayers originated from the echinoderms: II. Binary systems of Cerebrosides and steroids
Colloids and surfaces. B Biointerfaces, 2005Co-Authors: Hiromichi Nakahara, Masanori Inagaki, Ryuichi Higuchi, Kazufumi Nakamura, Shohei Nakamura, Mariko Aso, Osamu ShibataAbstract:Abstract The surface pressure (π)–area (A), the surface potential (ΔV)–A and the dipole moment (μ⊥)–A isotherms were obtained for two-component monolayers of two different Cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (ΔV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort [J. Colloid Interf. Sci. 46 (1974) 191–202]. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (μ⊥) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (Xphospholipid), using the additivity rule. From the A–Xphospholipid and ΔVm–Xphospholipid plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of Cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of Cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the Cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
Masanori Inagaki - One of the best experts on this subject based on the ideXlab platform.
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langmur monolayers of cerebroside with different head groups originated from sea cucumber binary systems with dipalmitoylphosphatidylcholine dppc
Colloids and Surfaces B: Biointerfaces, 2009Co-Authors: Yuriko Ikeda, Masanori Inagaki, Ryuichi Higuchi, Tomofumi Miyamoto, Koji Yamada, Osamu ShibataAbstract:Surface properties (Langmuir monolayer) of two different Cerebrosides which are extracted from the sea cucumber (Bohadschia argus) were investigated. A main difference in chemical structure of cerebroside between BAC-2a and BAC-4 is their head groups (glucose and galactose, respectively). Furthermore, miscibility and interaction between dipalmitoylphosphatidylcholine (DPPC) and Cerebrosides (BAC-2a and BAC-4) in the monolayer have been systematically examined. The surface pressure (pi)-area (A), the surface potential (Delta V)-A, and the dipole moment (mu(perpendicular))-A isotherms for monolayers of DPPC, Cerebrosides, and their binary combinations have been measured using the Wilhelmy method and the ionizing electrode method. BAC-4 forms a stable liquid-expanded (LE) monolayer, whereas BAC-2a has a first-order phase transition from the LE phase to the liquid-condensed (LC) state on 0.15 M NaCl at 298.2 K. The fundamental properties for each cerebroside monolayer were elucidated in terms of the surface dipole moment based on the three-layer model [R.J. Demchak, T. Fort Jr., J. Colloid Interface Sci. 46 (1974) 191-202] for both Cerebrosides and the apparent molar quantity change (Delta s(gamma), Delta h(gamma), and Delta u(gamma)) for BAC-2a. In addition, their miscibility with DPPC was examined by the variation of the molecular areas and the surface potentials as a function of cerebroside mole fractions, the additivity rule. The miscibility was also confirmed by constructing the two-dimensional phase diagrams. The phase diagrams for the both binary systems were of negative azeotropic type. That is, the two-component DPPC/BAC-2a and DPPC/BAC-4 monolayers are miscible. Furthermore, the Joos equation for the analysis of the collapse pressure of binary monolayers allowed calculation of the interaction parameter and the interaction energy between the DPPC and cerebroside monolayers. The miscibility in the monolayer state was also confirmed by the morphological observation with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM).
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Biologically Active Glycosides from Asteroidea, 41. Isolation and Structure Determination of GlucoCerebrosides from the Starfish Linckia laevigata
Chemical & Pharmaceutical Bulletin, 2005Co-Authors: Tomoki Maruta, Masanori Inagaki, Osamu Shibata, Takeshi Saito, Ryuichi HiguchiAbstract:A new glucocerebroside, linckiacerebroside A (1) and a known glucocerebroside S-2a-3 (2), have been isolated from the cerebroside molecular species obtained from the less polar fraction of the CHCl3/MeOH extract of the starfish Linckia laevigata, together with three pseudo homogeneous glucocerebroside, 3, 4, and 5. The structures of these Cerebrosides were determined on the basis of chemical and spectroscopic evidence.
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Langmuir monolayers of cerebroside originated from Linckia laevigata : Binary systems of Cerebrosides and phospholipid
Colloids and Surfaces B: Biointerfaces, 2005Co-Authors: Tomoki Maruta, Masanori Inagaki, Ryuichi Higuchi, Kazuki Hoda, Osamu ShibataAbstract:Abstract The surface pressure (π)–area (A), the surface potential (ΔV)–A and the dipole moment (μ⊥)–A isotherms were obtained for six Cerebrosides of LLC-2, LLC-2-1, LLC-2-8, LLC-2-10, LLC-2-12, and LLC-2-15, which were isolated from Linckia laevigata, and two-component monolayers of two different Cerebrosides (LLC-2 and LLC-2-8) with phospholipid of dipalmitoylphosphatidylcholine (DPPC) on a subphase of 0.15 M sodium chloride solution as a function of cerebroside compositions in the two-component systems by employing the Wilhelmy method, the ionizing electrode method, and the fluorescence microscopy. The new finding was that LLC-2 showed a stable and liquid expanded type film. Four of them (LLC-2-8, -10, -12, and -15) had the phase transition from the liquid-expanded (LE) to the liquid-condensed (LC) states at 298.2 K. The apparent molar quantity changes (Δsγ, Δhγ, and Δuγ) on their phase transition on 0.15 M at 298.2 K were calculated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the cerebroside molar fraction (Xcerebroside), using the additivity rule. From the A–Xcerebroside and ΔVm–Xphospholipid plots, a partial molecular surface area (PMA) and an apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible systems. Judging from the two-dimensional phase diagrams, these were found to be one type, a positive azeotropic type; all the Cerebrosides were miscible with DPPC. Furthermore, assuming a regular surface mixture, the Joos equation for the analysis of the collapse pressure of two-component monolayers allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the Cerebrosides and DPPC. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
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Cerebroside Langmuir monolayers originated from the echinoderms
Colloids and surfaces. B Biointerfaces, 2005Co-Authors: Hiromichi Nakahara, Masanori Inagaki, Ryuichi Higuchi, Kazufumi Nakamura, Shohei Nakamura, Mariko Aso, Osamu ShibataAbstract:The surface pressure (pi)-area (A), the surface potential (DeltaV)-A and the dipole moment (mu( perpendicular))-A isotherms were obtained for two-component monolayers of two different Cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (DeltaV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (mu( perpendicular)) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (X(phospholipid)), using the additivity rule. From the A-X(phospholipid) and DeltaV(m)-X(phospholipid) plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of Cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of Cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (xi) and the interaction energy (-Delta epsilon) between the Cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.
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Cerebroside Langmuir monolayers originated from the echinoderms: II. Binary systems of Cerebrosides and steroids
Colloids and surfaces. B Biointerfaces, 2005Co-Authors: Hiromichi Nakahara, Masanori Inagaki, Ryuichi Higuchi, Kazufumi Nakamura, Shohei Nakamura, Mariko Aso, Osamu ShibataAbstract:Abstract The surface pressure (π)–area (A), the surface potential (ΔV)–A and the dipole moment (μ⊥)–A isotherms were obtained for two-component monolayers of two different Cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (ΔV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort [J. Colloid Interf. Sci. 46 (1974) 191–202]. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (μ⊥) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (Xphospholipid), using the additivity rule. From the A–Xphospholipid and ΔVm–Xphospholipid plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of Cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of Cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the Cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.