The Experts below are selected from a list of 3786 Experts worldwide ranked by ideXlab platform
F. D. Alonso - One of the best experts on this subject based on the ideXlab platform.
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use of a crude extract or purified antigen from first instar cattle grubs hypoderma lineatum for the detection of anti hypoderma antibodies in free ranging cervids from southern spain
Medical and Veterinary Entomology, 2010Co-Authors: Rosario Panadero, Luis Leonvizcaino, C Martinezcarrasco, Maria Patrocinio Morrondo, Pablo Diezbanos, C. Lopez, F. D. AlonsoAbstract:During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
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Use of a crude extract or purified antigen from first‐instar cattle grubs, Hypoderma lineatum, for the detection of anti‐Hypoderma antibodies in free‐ranging cervids from southern Spain
Medical and Veterinary Entomology, 2010Co-Authors: Rosario Panadero, Maria Patrocinio Morrondo, Carlos Martínez-carrasco, Luis León-vizcaíno, Pablo Díez-baños, C. Lopez, F. D. AlonsoAbstract:During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
Rosario Panadero - One of the best experts on this subject based on the ideXlab platform.
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use of a crude extract or purified antigen from first instar cattle grubs hypoderma lineatum for the detection of anti hypoderma antibodies in free ranging cervids from southern spain
Medical and Veterinary Entomology, 2010Co-Authors: Rosario Panadero, Luis Leonvizcaino, C Martinezcarrasco, Maria Patrocinio Morrondo, Pablo Diezbanos, C. Lopez, F. D. AlonsoAbstract:During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
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Use of a crude extract or purified antigen from first‐instar cattle grubs, Hypoderma lineatum, for the detection of anti‐Hypoderma antibodies in free‐ranging cervids from southern Spain
Medical and Veterinary Entomology, 2010Co-Authors: Rosario Panadero, Maria Patrocinio Morrondo, Carlos Martínez-carrasco, Luis León-vizcaíno, Pablo Díez-baños, C. Lopez, F. D. AlonsoAbstract:During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
Emmanuel J P Douzery - One of the best experts on this subject based on the ideXlab platform.
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new phylogenetic perspectives on the Cervidae artiodactyla are provided by the mitochondrial cytochrome b gene
Proceedings of The Royal Society B: Biological Sciences, 1998Co-Authors: Ettore Randi, Nadia Mucci, M Pierpaoli, Emmanuel J P DouzeryAbstract:The entire mitochondrial cytochrome b (cyt b) gene was compared for 11 species of the artiodactyl family Cervidae, representing all living subfamilies, i.e. the antlered Cervinae (Cervus elaphus, C...
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The mitochondrial control region of Cervidae: evolutionary patterns and phylogenetic content.
Molecular biology and evolution, 1997Co-Authors: Emmanuel J P Douzery, Ettore RandiAbstract:The mitochondrial control region (CR) sequence, also known as the D-loop, has been determined for six Cervidae (Artiodactyla, Ruminantia): the red and fallow deers (subfamily Cervinae), the brocket deer and two roe deers (subfamily Odocoileinae), and the Chinese water deer (Hydropotinae). These new sequences have been aligned with available cervid and bovid orthologues. Comparative analyses indicate that the 5'-peripheral domain exhibits a 75-bp length polymorphism near sequences associated with the termination of the H-strand replication. The New World Odocoileinae possess the longest cervid CR due to the presence of an additional 47-bp tandem repeat, located in the 3'-peripheral domain, downstream of the initiation site for H-strand replication (OH) and the first conserved sequence block (CSB-1). This insertion represents a duplication spanning the OH to CSB-1 region and constitutes an exclusive synapomorphy for New World Odocoileinae. Phylogenetic analyses of the complete CR support the paraphyly of antlered deers due to the nesting of the antlerless Hydropotes within Odocoileinae. Capreolus is the closest relative of Hydropotes, and the divergence of this Old World Odocoileinae clade may have occurred between 8.7 and 10.4 MYA. The conserved central domain of CR can be aligned across ungulates and indicates the Pecora monophyly, their close association with cetaceans, and the earlier emergence of suiformes.
Ettore Randi - One of the best experts on this subject based on the ideXlab platform.
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new phylogenetic perspectives on the Cervidae artiodactyla are provided by the mitochondrial cytochrome b gene
Proceedings of The Royal Society B: Biological Sciences, 1998Co-Authors: Ettore Randi, Nadia Mucci, M Pierpaoli, Emmanuel J P DouzeryAbstract:The entire mitochondrial cytochrome b (cyt b) gene was compared for 11 species of the artiodactyl family Cervidae, representing all living subfamilies, i.e. the antlered Cervinae (Cervus elaphus, C...
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The mitochondrial control region of Cervidae: evolutionary patterns and phylogenetic content.
Molecular biology and evolution, 1997Co-Authors: Emmanuel J P Douzery, Ettore RandiAbstract:The mitochondrial control region (CR) sequence, also known as the D-loop, has been determined for six Cervidae (Artiodactyla, Ruminantia): the red and fallow deers (subfamily Cervinae), the brocket deer and two roe deers (subfamily Odocoileinae), and the Chinese water deer (Hydropotinae). These new sequences have been aligned with available cervid and bovid orthologues. Comparative analyses indicate that the 5'-peripheral domain exhibits a 75-bp length polymorphism near sequences associated with the termination of the H-strand replication. The New World Odocoileinae possess the longest cervid CR due to the presence of an additional 47-bp tandem repeat, located in the 3'-peripheral domain, downstream of the initiation site for H-strand replication (OH) and the first conserved sequence block (CSB-1). This insertion represents a duplication spanning the OH to CSB-1 region and constitutes an exclusive synapomorphy for New World Odocoileinae. Phylogenetic analyses of the complete CR support the paraphyly of antlered deers due to the nesting of the antlerless Hydropotes within Odocoileinae. Capreolus is the closest relative of Hydropotes, and the divergence of this Old World Odocoileinae clade may have occurred between 8.7 and 10.4 MYA. The conserved central domain of CR can be aligned across ungulates and indicates the Pecora monophyly, their close association with cetaceans, and the earlier emergence of suiformes.
C. Lopez - One of the best experts on this subject based on the ideXlab platform.
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use of a crude extract or purified antigen from first instar cattle grubs hypoderma lineatum for the detection of anti hypoderma antibodies in free ranging cervids from southern spain
Medical and Veterinary Entomology, 2010Co-Authors: Rosario Panadero, Luis Leonvizcaino, C Martinezcarrasco, Maria Patrocinio Morrondo, Pablo Diezbanos, C. Lopez, F. D. AlonsoAbstract:During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
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Use of a crude extract or purified antigen from first‐instar cattle grubs, Hypoderma lineatum, for the detection of anti‐Hypoderma antibodies in free‐ranging cervids from southern Spain
Medical and Veterinary Entomology, 2010Co-Authors: Rosario Panadero, Maria Patrocinio Morrondo, Carlos Martínez-carrasco, Luis León-vizcaíno, Pablo Díez-baños, C. Lopez, F. D. AlonsoAbstract:During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.