The Experts below are selected from a list of 5499 Experts worldwide ranked by ideXlab platform
Ana Paula Farina - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis an in vitro study
Journal of Endodontics, 2014Co-Authors: Ana Paula De Almeida, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, Daniela Cristina Miyagaki, Yuri Dal BelloAbstract:Abstract Introduction The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl] 2 ) and sodium hypochlorite (NaOCl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis . Methods The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis , and incubated for 30 days. The samples were divided into 6 groups ( n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl) 2 ; G5: 2.5% NaOCl with ultrasonic activation; and G6: 2.5% Ca(OCl) 2 with ultrasonic activation (US). Microbiological testing (colony-forming unit [CFU] Counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05). Results Groups 1 and 2 showed the highest mean contamination (3.26 log 10 CFU/mL and 2.69 log 10 CFU/mL, respectively), which was statistically different from all other groups ( P 2 + US) showed the lowest mean contamination (1.00 log 10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl] 2 ), and 5 (NaOCl + US) ( P Conclusions Ca(OCl) 2 as well as passive ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.
P R Donald - One of the best experts on this subject based on the ideXlab platform.
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time to liquid culture positivity can substitute for colony Counting on agar plates in early bactericidal activity studies of antituberculosis agents
Clinical Microbiology and Infection, 2012Co-Authors: Andreas H Diaco, J S Maritz, Amou Vente, P D Van Helde, Rod Dawso, P R DonaldAbstract:Abstract The measurement of early bactericidal activity (EBA) is the first step in the clinical investigation of antituberculosis agents. EBA is determined by quantifying the viable sputum mycobacterial load on consecutive days of treatment. To investigate whether time to positivity (TTP) in mycobacterial liquid culture can substitute for colony forming unit (CFU) Counting on agar plates we compared the error variation of TTP and CFU in 2115 pooled sputum samples collected overnight from 250 individuals included in five EBA studies. We found that the technical variation between duplicate laboratory measurements and the within-subject or day-to-day variation were similar for TTP (8.5% and 27.4% of total variation, respectively) and CFU (6.7% and 29.3% of total variation). The ability of the measurements to separate the EBA of 22 treatment arms was determined with group rank correlation of means and one-way analysis of variance. Except for the EBA over 0-2 days, individual and group EBAs measured with TTP and CFU were highly correlated. Treatment group means rank correlation coefficients were r = 0.472, r = 0.910 and r = 0.818, respectively, for EBA 0-2 days, EBA 0-7 days and EBA 0-14 days. Analysis of variance significantly favoured TTP over CFU for discrimination between groups with F values of 6.58 and 1.87, 7.77 and 4.58, and 8.71 and 3.56, respectively. We conclude that TTP is an acceptable alternative to CFU Counting for the determination of the viable sputum mycobacterial load in EBA studies of up to 14 days duration.
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time to detection of the growth of mycobacterium tuberculosis in mgit 960 for determining the early bactericidal activity of antituberculosis agents
European Journal of Clinical Microbiology & Infectious Diseases, 2010Co-Authors: Andreas H Diacon, J S Maritz, Amour Venter, P D Van Helden, K Andries, D F Mcneeley, P R DonaldAbstract:Evaluation of early bactericidal activity (EBA) by the determination of a fall in viable colony-forming units (CFU) of Mycobacterium tuberculosis in sputum is a first step in the clinical study of new antituberculosis agents. The time to detection (TTD) of growth in liquid media is more sensitive and could substitute for CFU Counting on solid media. Overnight sputum samples collected during the evaluation of the novel agent TMC207 in comparison to isoniazid and rifampicin were studied. For the determination of CFU, we incubated 10-fold dilutions of homogenized sputum on selective 7H10 agar. The TTD was measured by incubating decontaminated sputum in the BACTEC MGIT 960 system. The fall in bacillary load over 7 days determined by CFU Counting closely matched the prolongation of the TTD in the BACTEC MGIT 960 system. The CFU counts correlated significantly with the TTD. While the ranking of agents and different dosages of TMC207 was similar, the highest dose of TMC207 showed markedly better activity when measured by the TTD than CFU Counting when compared to the activity of isoniazid. Automated TTD could augment, or, in future, replace, CFU Counting to determine sputum bacillary load in EBA clinical trials pending a more formal evaluation of the correlation of the measurements.
K. Becker - One of the best experts on this subject based on the ideXlab platform.
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cold plasma therapy of a tooth root canal infected with enterococcus faecalis biofilms in vitro
Journal of Endodontics, 2013Co-Authors: Yongdong Liang, Xiaohui Yang, Jue Zhang, Jing Fang, Jing Wang, K. BeckerAbstract:Abstract Introduction Complete sterilization of an infected root canal is an important challenge in endodontic treatment. Traditional methods often cannot achieve high-efficiency sterilization because of the complexity of the root canal system. The objective of the study was to investigate in vitro the feasibility of using a cold plasma treatment of a root canal infected with Enterococcus faecalis biofilms. Methods Seventy single-root teeth infected with E. faecalis biofilms were divided into 7 groups. Group 1 served as the negative control group (no treatment), and group 7 was the positive control group with teeth treated with calcium hydroxide intracanal medication for 7 days. Groups 2 to 6 included teeth treated by cold plasma for 2, 4, 6, 8, and 10 minutes, respectively. The disinfection of the E. faecalis biofilm was evaluated by colony-forming unit (CFU) Counting. Scanning electron microscopy was used to evaluate the structural changes of the E. faecalis biofilm before and after plasma treatment. Confocal scanning laser microscopy was used to investigate the vitality of the microorganisms in the biofilm before and after plasma treatment. Results A significant decrease in the number of CFUs was observed after prolonged cold plasma treatment (based on the statistical analysis of the teeth in groups 2–6). Compared with the positive control group, cold plasma treatment of 8 or 10 minutes (groups 5 and 6) had a significantly higher antimicrobial efficacy ( P E. faecalis death and destruction of the biofilm. Conclusions The cold plasma had a high efficiency in disinfecting the E. faecalis biofilms in in vitro dental root canal treatment.
Doglas Cecchin - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis an in vitro study
Journal of Endodontics, 2014Co-Authors: Ana Paula De Almeida, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, Daniela Cristina Miyagaki, Yuri Dal BelloAbstract:Abstract Introduction The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl] 2 ) and sodium hypochlorite (NaOCl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis . Methods The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis , and incubated for 30 days. The samples were divided into 6 groups ( n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl) 2 ; G5: 2.5% NaOCl with ultrasonic activation; and G6: 2.5% Ca(OCl) 2 with ultrasonic activation (US). Microbiological testing (colony-forming unit [CFU] Counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05). Results Groups 1 and 2 showed the highest mean contamination (3.26 log 10 CFU/mL and 2.69 log 10 CFU/mL, respectively), which was statistically different from all other groups ( P 2 + US) showed the lowest mean contamination (1.00 log 10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl] 2 ), and 5 (NaOCl + US) ( P Conclusions Ca(OCl) 2 as well as passive ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.
Yongdong Liang - One of the best experts on this subject based on the ideXlab platform.
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cold plasma therapy of a tooth root canal infected with enterococcus faecalis biofilms in vitro
Journal of Endodontics, 2013Co-Authors: Yongdong Liang, Xiaohui Yang, Jue Zhang, Jing Fang, Jing Wang, K. BeckerAbstract:Abstract Introduction Complete sterilization of an infected root canal is an important challenge in endodontic treatment. Traditional methods often cannot achieve high-efficiency sterilization because of the complexity of the root canal system. The objective of the study was to investigate in vitro the feasibility of using a cold plasma treatment of a root canal infected with Enterococcus faecalis biofilms. Methods Seventy single-root teeth infected with E. faecalis biofilms were divided into 7 groups. Group 1 served as the negative control group (no treatment), and group 7 was the positive control group with teeth treated with calcium hydroxide intracanal medication for 7 days. Groups 2 to 6 included teeth treated by cold plasma for 2, 4, 6, 8, and 10 minutes, respectively. The disinfection of the E. faecalis biofilm was evaluated by colony-forming unit (CFU) Counting. Scanning electron microscopy was used to evaluate the structural changes of the E. faecalis biofilm before and after plasma treatment. Confocal scanning laser microscopy was used to investigate the vitality of the microorganisms in the biofilm before and after plasma treatment. Results A significant decrease in the number of CFUs was observed after prolonged cold plasma treatment (based on the statistical analysis of the teeth in groups 2–6). Compared with the positive control group, cold plasma treatment of 8 or 10 minutes (groups 5 and 6) had a significantly higher antimicrobial efficacy ( P E. faecalis death and destruction of the biofilm. Conclusions The cold plasma had a high efficiency in disinfecting the E. faecalis biofilms in in vitro dental root canal treatment.