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Rafael Cisneros - One of the best experts on this subject based on the ideXlab platform.
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effect of sonic and Ultrasonic Activation on organic tissue dissolution from simulated grooves in root canals using sodium hypochlorite and edta
International Endodontic Journal, 2017Co-Authors: Antonio J Conde, Roberto Estevez, Giampiero Rossifedele, Gaizka Lorono, Valencia O De Pablo, Rafael CisnerosAbstract:Aim To compare soft-tissue dissolution by sodium hypochlorite, with an EDTA intermediate rinse, with or without Activation with passive Ultrasonic Activation (PUI) or sonic Activation using the Endoactivator (EA) or Eddy tips (ED). Methodology The root canals of eighty-three human maxillary central incisors were chemo-mechanically prepared and the teeth split. A standardized longitudinal intracanal groove was created in one of the root halves. Eighty-three porcine palatal mucosa samples were collected, adapted to fit into the grooves and weighed. The re-assembled specimens were randomly divided into four experimental groups (n = 20), based on the final rinse: no Activation; EA; PUI; ED, using 2.5% sodium hypochlorite, with an EDTA intermediate rinse. A control group (n = 3) was irrigated with distilled water without Activation. The solutions were delivered using a syringe and needle 2 mm from working length. Total irrigation time was 150 s, including 60 s of Activation in the specific groups. The study was carried out at 36 ± 2 °C. The porcine palatal mucosa samples were weighed after completion of the assays. Student paired t-test and anova were used to assess the intra- and intergroup weight changes. The multiple comparisons were evaluated using Bonferroni correction (α = 0.05). Results Weight loss occurred in all experimental groups. Irrigant Activation resulted in greater weight loss when compared to the nonactivated group [vs. EA (P = 0.001); vs. PUI (P < 0.001); vs. ED (P < 0.001)]. No significant differences were found amongst the different Activation systems. Conclusions Activation increased the tissue-dissolving activity of irrigants from artificial grooves in root canals of maxillary central incisors.
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effect of passive Ultrasonic Activation on organic tissue dissolution from simulated grooves in root canals using sodium hypochlorite with or without surfactants and edta
Journal of Endodontics, 2017Co-Authors: Roberto Estevez, Antonio J Conde, Oliver Valencia De Pablo, Francisco De La Torre, Giampiero Rossifedele, Rafael CisnerosAbstract:Abstract Introduction The aim of this study was to evaluate the porcine palatal mucosa dissolution from artificial grooves using a final rinse with sodium hypochlorite (NaOCl) with or without a surfactant or Ultrasonic Activation (PUI). Methods The root canals of 130 human maxillary central incisors were chemomechanically prepared and the teeth split. A standardized longitudinal intracanal groove was created in 1 of the root halves. One hundred thirty porcine palatal mucosa samples were collected, adapted in order to fit into the grooves, and weighed. The reassembled specimens were randomly divided in 3 experimental groups (n = 40) based on their irrigation protocol (ie, positive pressure [PP] and PUI during 15 [PUI-15] or 30 seconds [PUI-30]) and divided in subgroups according to the NaOCl preparation used: Vista 6% plain (Vista Dental Products, Racine, WI) or Chlor-XTRA (Vista Dental Products) (containing surfactant). An EDTA intermediate rinse was included. Palatal mucosa weights were measured after the assays. The intergroup weight changes were statistically analyzed. Results Complete dissolution did not occur in any sample. Chlor-XTRA subgroups were associated with increased weight reduction compared with Vista 6% plain subgroups; however, the differences were significant (P Conclusions The addition of a surfactant to NaOCl and/or PUI Activation increased palatal mucosa dissolution within artificial grooves in the root canal of incisor teeth. PUI agitation was often able to compensate for the absence of surfactants.
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root canal penetration of a sodium hypochlorite mixture using sonic or Ultrasonic Activation
Brazilian Dental Journal, 2014Co-Authors: Marta Sainzpardo, Roberto Estevez, Oliver Valencia De Pablo, Giampiero Rossifedele, Rafael CisnerosAbstract:The purpose of this ex vivo study was to determine, in "open" and "closed" systems, whether the design has an influence on the penetration length of sodium hypochlorite mixed with a radiopaque contrast medium, measured in millimeters, when delivered using positive pressure (PP) and using sonic (SI) or passive Ultrasonic (PUI) Activation. Sixty single-rooted teeth were divided into two groups: open and closed systems (n=30). Root canal shaping was performed to a working length of 17 mm. The samples were divided into three sub-groups (n=10) according to irrigant delivery and Activation: PP, and SI or PUI Activation. By using radiographs, penetration length was measured, and vapor lock was assessed. For the closed group, the penetration distance means were: PP 15.715 (±0.898) mm, SI 16.299 (±0.738) mm and PUI 16.813 (±0.465) mm, with vapor lock occurring in 53.3% of the specimens. In the open group, penetration to 17 mm occurred in 97.6% of the samples, and no vapor lock occurred. Irrigant penetration and distribution evaluation using open and closed systems provide significantly different results. For closed systems, PUI is the most effective in delivering the irrigant to working length, followed by SI.
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effect of edta sonic and Ultrasonic Activation on the penetration of sodium hypochlorite into simulated lateral canals an in vitro study
Journal of Endodontics, 2009Co-Authors: Cesar De Gregorio, Roberto Estevez, Rafael Cisneros, Carlos Heilborn, Nestor CohencaAbstract:Abstract Introduction The purpose of this study was to evaluate the penetration of 5.25% sodium hypochlorite alone or in combination with 17% EDTA in simulated lateral canals using sonic and Ultrasonic Activation. Methods Four hundred and eighty simulated lateral canals were created in 80 single rooted cleared teeth by inserting 06 K-files at 2, 4.5 and 6 mm of working length. Samples were mounted on clear silicon to simulate the presence of surrounding periodontal tissues and its effects on fluid dynamics and then randomly assigned to four experimental groups: 1 ( n = 20) 5.25% NaOCl + sonic Activation; 2 ( n = 20) 5.25% NaOCl + Ultrasonic Activation; 3 ( n = 20) 5.25% NaOCl + 17% EDTA + sonic Activation and 4 ( n = 20) 5.25% NaOCl + 17% EDTA + Ultrasonic Activation. Sonic Activation was delivered using the Endoactivator® inserted 2 mm short of working length and activated for 1 minute. Ultrasonic Activation was performed with a stainless steel Ultrasonic file inserted 2 mm short of working length and passively activated for 3 cycles of 20 seconds each. Samples were evaluated by direct observation of the images recorded under the operating microscope and by radiographic evaluation after irrigation with a contrast solution. Results Sonic and Ultrasonic Activation resulted in a better irrigation of the lateral canals at 4.5 and 2 mm from working length compared to traditional needle irrigation alone. Traditional needle irrigation alone demonstrated significantly less penetration of irrigant into the lateral canals and was limited to the level of penetration of the needle. Conclusion The addition of EDTA did not result in better penetration of irrigants into the lateral canals.
Ana Paula Farina - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive Ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis an in vitro study
Journal of Endodontics, 2014Co-Authors: Ana Paula De Almeida, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, Daniela Cristina Miyagaki, Yuri Dal BelloAbstract:Abstract Introduction The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl] 2 ) and sodium hypochlorite (NaOCl) associated with passive Ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis . Methods The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis , and incubated for 30 days. The samples were divided into 6 groups ( n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl) 2 ; G5: 2.5% NaOCl with Ultrasonic Activation; and G6: 2.5% Ca(OCl) 2 with Ultrasonic Activation (US). Microbiological testing (colony-forming unit [CFU] counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05). Results Groups 1 and 2 showed the highest mean contamination (3.26 log 10 CFU/mL and 2.69 log 10 CFU/mL, respectively), which was statistically different from all other groups ( P 2 + US) showed the lowest mean contamination (1.00 log 10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl] 2 ), and 5 (NaOCl + US) ( P Conclusions Ca(OCl) 2 as well as passive Ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.
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Influence of Ultrasonic Activation on photodynamic therapy over root canal system infected with Enterococcus faecalis--an in vitro study.
Photodiagnosis and photodynamic therapy, 2014Co-Authors: Guilherme Cavagnoli Ghinzelli, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, José Antônio Poli De FigueiredoAbstract:Summary Aim The purpose of this study was to evaluate, in vitro, the influence of Ultrasonic Activation on photodynamic therapy over root canal system infected with Enterococcus faecalis. Material and methods The root canals of 50 single-rooted human extracted teeth were enlarged up to a file 60, autoclaved, inoculated with Enterococcus faecalis and incubated for 30 days. The samples were divided into five groups (n = 10) according to the protocol of decontamination: G1 (control group) – no procedure was performed; G2 – photosensitizer (0.01% methylene blue); G3 – Ultrasonic Activation of photosensitizer (0.01% methylene blue); G4 – photodynamic therapy with no Ultrasonic Activation; and G5 – photodynamic therapy with Ultrasonic Activation. Microbiological tests (CFU counting) and scanning electron microscopy (SEM) were performed to evaluate and illustrate, respectively, the effectiveness of proposed treatments. Data were subjected to one-way ANOVA followed by post hoc Tukey test (α = 0.05). Results The microbiological test demonstrated that G5 (photodynamic therapy with Ultrasonic Activation) showed the lowest mean contamination (3.17 log CFU/mL), which was statistically different from all other groups (p Conclusion The use of Ultrasonic Activation on photodynamic therapy improved its potential for decontamination, resulting in the higher elimination Enterococcus faecalis from the root canal space.
Doglas Cecchin - One of the best experts on this subject based on the ideXlab platform.
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influence of Ultrasonic Activation over final irrigants in the removal of photosensitizer from root canal walls after photodynamic therapy
Photodiagnosis and Photodynamic Therapy, 2017Co-Authors: Matheus Albino Souza, Doglas Cecchin, Huriel Scartazzini Palhano, Bianca Pazinatto, Karolina Frick Bischoff, José Antônio Poli De FigueiredoAbstract:Abstract Introduction The aim of the present study was to evaluate the influence of Ultrasonic Activation (US) of the final irrigant in the removal of the photosensitizer from root canal walls after photodynamic therapy. Methods The root canals of 60 single-rooted bovine extracted teeth were filled with 0.01% methylene blue and submitted to photodynamic therapy for 90 s. After that, the roots were divided into six groups (n = 10) according to the final irrigation protocol: distilled water (DW), DW + US, 17% EDTA, QMix, EDTA + US, and QMix + US. Then, the samples were submitted to scanning electron microscopy where a scoring system was used to evaluate the images and effectiveness of proposed treatments in the cervical, middle and apical regions of the root canals. The data were statistically analyzed by the Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% of significance. Results The 17% EDTA + US and QMix + US treatments were most effective in the removal of the photosensitizer in all regions of the root canal; the difference was statistically significant when compared to all other groups (p Conclusions US can aid 17% EDTA and QMix in removing the photosensitizer after photodynamic therapy and contributes to cleaning root canal walls.
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influence of Ultrasonic Activation in association with different final irrigants on intracanal smear layer removal
Brazilian Journal of Oral Sciences, 2016Co-Authors: Matheus Albino Souza, Felipe Trentin Motter, Tobias Pitton Fontana, Marlos Barbosa Ribeiro, Daniela Cristina Miyagaki, Doglas CecchinAbstract:Aim: To evaluate the influence of Ultrasonic Activation (US) with different irrigant regimens in smear layer removal. Methods: One hundred bovine incisors were instrumented and divided into ten groups (n=10) according to final irrigation protocols: distilled water (DW); DW+US; 17% EDTA; QMix; 10% citric acid; 37% phosphoric acid; 17% EDTA+US; QMix+US; 10% citric acid+US; 37% phosphoric acid+US. The samples were then submitted to scanning electron microscopy where a score system was used to evaluate the images and effectiveness of proposed treatments. The data were statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% significance level. Results: In the cervical third, groups 17% EDTA, QMix, 10% citric acid, 17% EDTA+US, QMix+US and 10% citric acid+US were more effective in smear layer removal (p<0.05); in the middle third, groups 17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05); in the apical third, groups 17% EDTA,17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05). Conclusions: US can aid 17% EDTA and QMix in smear layer removal at the middle third and QMix at the apical third, contributing to the cleaning of root canal system.
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comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive Ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis an in vitro study
Journal of Endodontics, 2014Co-Authors: Ana Paula De Almeida, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, Daniela Cristina Miyagaki, Yuri Dal BelloAbstract:Abstract Introduction The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl] 2 ) and sodium hypochlorite (NaOCl) associated with passive Ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis . Methods The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis , and incubated for 30 days. The samples were divided into 6 groups ( n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl) 2 ; G5: 2.5% NaOCl with Ultrasonic Activation; and G6: 2.5% Ca(OCl) 2 with Ultrasonic Activation (US). Microbiological testing (colony-forming unit [CFU] counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05). Results Groups 1 and 2 showed the highest mean contamination (3.26 log 10 CFU/mL and 2.69 log 10 CFU/mL, respectively), which was statistically different from all other groups ( P 2 + US) showed the lowest mean contamination (1.00 log 10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl] 2 ), and 5 (NaOCl + US) ( P Conclusions Ca(OCl) 2 as well as passive Ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.
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Influence of Ultrasonic Activation on photodynamic therapy over root canal system infected with Enterococcus faecalis--an in vitro study.
Photodiagnosis and photodynamic therapy, 2014Co-Authors: Guilherme Cavagnoli Ghinzelli, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, José Antônio Poli De FigueiredoAbstract:Summary Aim The purpose of this study was to evaluate, in vitro, the influence of Ultrasonic Activation on photodynamic therapy over root canal system infected with Enterococcus faecalis. Material and methods The root canals of 50 single-rooted human extracted teeth were enlarged up to a file 60, autoclaved, inoculated with Enterococcus faecalis and incubated for 30 days. The samples were divided into five groups (n = 10) according to the protocol of decontamination: G1 (control group) – no procedure was performed; G2 – photosensitizer (0.01% methylene blue); G3 – Ultrasonic Activation of photosensitizer (0.01% methylene blue); G4 – photodynamic therapy with no Ultrasonic Activation; and G5 – photodynamic therapy with Ultrasonic Activation. Microbiological tests (CFU counting) and scanning electron microscopy (SEM) were performed to evaluate and illustrate, respectively, the effectiveness of proposed treatments. Data were subjected to one-way ANOVA followed by post hoc Tukey test (α = 0.05). Results The microbiological test demonstrated that G5 (photodynamic therapy with Ultrasonic Activation) showed the lowest mean contamination (3.17 log CFU/mL), which was statistically different from all other groups (p Conclusion The use of Ultrasonic Activation on photodynamic therapy improved its potential for decontamination, resulting in the higher elimination Enterococcus faecalis from the root canal space.
Matheus Albino Souza - One of the best experts on this subject based on the ideXlab platform.
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influence of Ultrasonic Activation using different final irrigants on antimicrobial activity smear layer removal and bond strength of filling material
Australian Endodontic Journal, 2019Co-Authors: Matheus Albino Souza, Igor Paim Hoffmann, Vitor Hugo Sanches Menchik, Julia Zandona, Caroline Tumelero Dias, Huriel Scartazzini Palhano, Charise Dallazem Bertol, Luciana Grazziotin RossatograndoAbstract:The aim of this study was to evaluate the influence of Ultrasonic Activation (US) over final irrigants on antimicrobial activity, smear layer removal and bond strength. The root canals of 180 extracted human teeth were distributed into three experimental tests (n = 60). In each test, the samples were subdivided into six groups (n = 10) according to final irrigation protocols: G1:distilled water (DW); G2:DW + US; G3:17% EDTA; G4:QMix; G5:17% EDTA + US; and G6:QMix + US. The antimicrobial activity was evaluated by counting of colony-forming units (CFUs), the smear layer removal by scanning electron microscopy and the bond strength by push-out test. Statistical analysis was performed for each evaluation (α = 5%). Groups 4 and 6 provided higher bacterial reduction, groups 5 and 6 provided higher smear layer removal, groups 3, 4, 5 and 6 provided the higher bond strength (P < 0.05). US Activation did not improve the antimicrobial activity and the bond strength, whereas it improved the smear layer removal.
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influence of Ultrasonic Activation over final irrigants in the removal of photosensitizer from root canal walls after photodynamic therapy
Photodiagnosis and Photodynamic Therapy, 2017Co-Authors: Matheus Albino Souza, Doglas Cecchin, Huriel Scartazzini Palhano, Bianca Pazinatto, Karolina Frick Bischoff, José Antônio Poli De FigueiredoAbstract:Abstract Introduction The aim of the present study was to evaluate the influence of Ultrasonic Activation (US) of the final irrigant in the removal of the photosensitizer from root canal walls after photodynamic therapy. Methods The root canals of 60 single-rooted bovine extracted teeth were filled with 0.01% methylene blue and submitted to photodynamic therapy for 90 s. After that, the roots were divided into six groups (n = 10) according to the final irrigation protocol: distilled water (DW), DW + US, 17% EDTA, QMix, EDTA + US, and QMix + US. Then, the samples were submitted to scanning electron microscopy where a scoring system was used to evaluate the images and effectiveness of proposed treatments in the cervical, middle and apical regions of the root canals. The data were statistically analyzed by the Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% of significance. Results The 17% EDTA + US and QMix + US treatments were most effective in the removal of the photosensitizer in all regions of the root canal; the difference was statistically significant when compared to all other groups (p Conclusions US can aid 17% EDTA and QMix in removing the photosensitizer after photodynamic therapy and contributes to cleaning root canal walls.
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influence of Ultrasonic Activation in association with different final irrigants on intracanal smear layer removal
Brazilian Journal of Oral Sciences, 2016Co-Authors: Matheus Albino Souza, Felipe Trentin Motter, Tobias Pitton Fontana, Marlos Barbosa Ribeiro, Daniela Cristina Miyagaki, Doglas CecchinAbstract:Aim: To evaluate the influence of Ultrasonic Activation (US) with different irrigant regimens in smear layer removal. Methods: One hundred bovine incisors were instrumented and divided into ten groups (n=10) according to final irrigation protocols: distilled water (DW); DW+US; 17% EDTA; QMix; 10% citric acid; 37% phosphoric acid; 17% EDTA+US; QMix+US; 10% citric acid+US; 37% phosphoric acid+US. The samples were then submitted to scanning electron microscopy where a score system was used to evaluate the images and effectiveness of proposed treatments. The data were statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% significance level. Results: In the cervical third, groups 17% EDTA, QMix, 10% citric acid, 17% EDTA+US, QMix+US and 10% citric acid+US were more effective in smear layer removal (p<0.05); in the middle third, groups 17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05); in the apical third, groups 17% EDTA,17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05). Conclusions: US can aid 17% EDTA and QMix in smear layer removal at the middle third and QMix at the apical third, contributing to the cleaning of root canal system.
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comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive Ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis an in vitro study
Journal of Endodontics, 2014Co-Authors: Ana Paula De Almeida, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, Daniela Cristina Miyagaki, Yuri Dal BelloAbstract:Abstract Introduction The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl] 2 ) and sodium hypochlorite (NaOCl) associated with passive Ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis . Methods The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis , and incubated for 30 days. The samples were divided into 6 groups ( n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl) 2 ; G5: 2.5% NaOCl with Ultrasonic Activation; and G6: 2.5% Ca(OCl) 2 with Ultrasonic Activation (US). Microbiological testing (colony-forming unit [CFU] counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05). Results Groups 1 and 2 showed the highest mean contamination (3.26 log 10 CFU/mL and 2.69 log 10 CFU/mL, respectively), which was statistically different from all other groups ( P 2 + US) showed the lowest mean contamination (1.00 log 10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl] 2 ), and 5 (NaOCl + US) ( P Conclusions Ca(OCl) 2 as well as passive Ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.
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Influence of Ultrasonic Activation on photodynamic therapy over root canal system infected with Enterococcus faecalis--an in vitro study.
Photodiagnosis and photodynamic therapy, 2014Co-Authors: Guilherme Cavagnoli Ghinzelli, Matheus Albino Souza, Doglas Cecchin, Ana Paula Farina, José Antônio Poli De FigueiredoAbstract:Summary Aim The purpose of this study was to evaluate, in vitro, the influence of Ultrasonic Activation on photodynamic therapy over root canal system infected with Enterococcus faecalis. Material and methods The root canals of 50 single-rooted human extracted teeth were enlarged up to a file 60, autoclaved, inoculated with Enterococcus faecalis and incubated for 30 days. The samples were divided into five groups (n = 10) according to the protocol of decontamination: G1 (control group) – no procedure was performed; G2 – photosensitizer (0.01% methylene blue); G3 – Ultrasonic Activation of photosensitizer (0.01% methylene blue); G4 – photodynamic therapy with no Ultrasonic Activation; and G5 – photodynamic therapy with Ultrasonic Activation. Microbiological tests (CFU counting) and scanning electron microscopy (SEM) were performed to evaluate and illustrate, respectively, the effectiveness of proposed treatments. Data were subjected to one-way ANOVA followed by post hoc Tukey test (α = 0.05). Results The microbiological test demonstrated that G5 (photodynamic therapy with Ultrasonic Activation) showed the lowest mean contamination (3.17 log CFU/mL), which was statistically different from all other groups (p Conclusion The use of Ultrasonic Activation on photodynamic therapy improved its potential for decontamination, resulting in the higher elimination Enterococcus faecalis from the root canal space.
Roberto Estevez - One of the best experts on this subject based on the ideXlab platform.
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effect of sonic and Ultrasonic Activation on organic tissue dissolution from simulated grooves in root canals using sodium hypochlorite and edta
International Endodontic Journal, 2017Co-Authors: Antonio J Conde, Roberto Estevez, Giampiero Rossifedele, Gaizka Lorono, Valencia O De Pablo, Rafael CisnerosAbstract:Aim To compare soft-tissue dissolution by sodium hypochlorite, with an EDTA intermediate rinse, with or without Activation with passive Ultrasonic Activation (PUI) or sonic Activation using the Endoactivator (EA) or Eddy tips (ED). Methodology The root canals of eighty-three human maxillary central incisors were chemo-mechanically prepared and the teeth split. A standardized longitudinal intracanal groove was created in one of the root halves. Eighty-three porcine palatal mucosa samples were collected, adapted to fit into the grooves and weighed. The re-assembled specimens were randomly divided into four experimental groups (n = 20), based on the final rinse: no Activation; EA; PUI; ED, using 2.5% sodium hypochlorite, with an EDTA intermediate rinse. A control group (n = 3) was irrigated with distilled water without Activation. The solutions were delivered using a syringe and needle 2 mm from working length. Total irrigation time was 150 s, including 60 s of Activation in the specific groups. The study was carried out at 36 ± 2 °C. The porcine palatal mucosa samples were weighed after completion of the assays. Student paired t-test and anova were used to assess the intra- and intergroup weight changes. The multiple comparisons were evaluated using Bonferroni correction (α = 0.05). Results Weight loss occurred in all experimental groups. Irrigant Activation resulted in greater weight loss when compared to the nonactivated group [vs. EA (P = 0.001); vs. PUI (P < 0.001); vs. ED (P < 0.001)]. No significant differences were found amongst the different Activation systems. Conclusions Activation increased the tissue-dissolving activity of irrigants from artificial grooves in root canals of maxillary central incisors.
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effect of passive Ultrasonic Activation on organic tissue dissolution from simulated grooves in root canals using sodium hypochlorite with or without surfactants and edta
Journal of Endodontics, 2017Co-Authors: Roberto Estevez, Antonio J Conde, Oliver Valencia De Pablo, Francisco De La Torre, Giampiero Rossifedele, Rafael CisnerosAbstract:Abstract Introduction The aim of this study was to evaluate the porcine palatal mucosa dissolution from artificial grooves using a final rinse with sodium hypochlorite (NaOCl) with or without a surfactant or Ultrasonic Activation (PUI). Methods The root canals of 130 human maxillary central incisors were chemomechanically prepared and the teeth split. A standardized longitudinal intracanal groove was created in 1 of the root halves. One hundred thirty porcine palatal mucosa samples were collected, adapted in order to fit into the grooves, and weighed. The reassembled specimens were randomly divided in 3 experimental groups (n = 40) based on their irrigation protocol (ie, positive pressure [PP] and PUI during 15 [PUI-15] or 30 seconds [PUI-30]) and divided in subgroups according to the NaOCl preparation used: Vista 6% plain (Vista Dental Products, Racine, WI) or Chlor-XTRA (Vista Dental Products) (containing surfactant). An EDTA intermediate rinse was included. Palatal mucosa weights were measured after the assays. The intergroup weight changes were statistically analyzed. Results Complete dissolution did not occur in any sample. Chlor-XTRA subgroups were associated with increased weight reduction compared with Vista 6% plain subgroups; however, the differences were significant (P Conclusions The addition of a surfactant to NaOCl and/or PUI Activation increased palatal mucosa dissolution within artificial grooves in the root canal of incisor teeth. PUI agitation was often able to compensate for the absence of surfactants.
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root canal penetration of a sodium hypochlorite mixture using sonic or Ultrasonic Activation
Brazilian Dental Journal, 2014Co-Authors: Marta Sainzpardo, Roberto Estevez, Oliver Valencia De Pablo, Giampiero Rossifedele, Rafael CisnerosAbstract:The purpose of this ex vivo study was to determine, in "open" and "closed" systems, whether the design has an influence on the penetration length of sodium hypochlorite mixed with a radiopaque contrast medium, measured in millimeters, when delivered using positive pressure (PP) and using sonic (SI) or passive Ultrasonic (PUI) Activation. Sixty single-rooted teeth were divided into two groups: open and closed systems (n=30). Root canal shaping was performed to a working length of 17 mm. The samples were divided into three sub-groups (n=10) according to irrigant delivery and Activation: PP, and SI or PUI Activation. By using radiographs, penetration length was measured, and vapor lock was assessed. For the closed group, the penetration distance means were: PP 15.715 (±0.898) mm, SI 16.299 (±0.738) mm and PUI 16.813 (±0.465) mm, with vapor lock occurring in 53.3% of the specimens. In the open group, penetration to 17 mm occurred in 97.6% of the samples, and no vapor lock occurred. Irrigant penetration and distribution evaluation using open and closed systems provide significantly different results. For closed systems, PUI is the most effective in delivering the irrigant to working length, followed by SI.
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effect of edta sonic and Ultrasonic Activation on the penetration of sodium hypochlorite into simulated lateral canals an in vitro study
Journal of Endodontics, 2009Co-Authors: Cesar De Gregorio, Roberto Estevez, Rafael Cisneros, Carlos Heilborn, Nestor CohencaAbstract:Abstract Introduction The purpose of this study was to evaluate the penetration of 5.25% sodium hypochlorite alone or in combination with 17% EDTA in simulated lateral canals using sonic and Ultrasonic Activation. Methods Four hundred and eighty simulated lateral canals were created in 80 single rooted cleared teeth by inserting 06 K-files at 2, 4.5 and 6 mm of working length. Samples were mounted on clear silicon to simulate the presence of surrounding periodontal tissues and its effects on fluid dynamics and then randomly assigned to four experimental groups: 1 ( n = 20) 5.25% NaOCl + sonic Activation; 2 ( n = 20) 5.25% NaOCl + Ultrasonic Activation; 3 ( n = 20) 5.25% NaOCl + 17% EDTA + sonic Activation and 4 ( n = 20) 5.25% NaOCl + 17% EDTA + Ultrasonic Activation. Sonic Activation was delivered using the Endoactivator® inserted 2 mm short of working length and activated for 1 minute. Ultrasonic Activation was performed with a stainless steel Ultrasonic file inserted 2 mm short of working length and passively activated for 3 cycles of 20 seconds each. Samples were evaluated by direct observation of the images recorded under the operating microscope and by radiographic evaluation after irrigation with a contrast solution. Results Sonic and Ultrasonic Activation resulted in a better irrigation of the lateral canals at 4.5 and 2 mm from working length compared to traditional needle irrigation alone. Traditional needle irrigation alone demonstrated significantly less penetration of irrigant into the lateral canals and was limited to the level of penetration of the needle. Conclusion The addition of EDTA did not result in better penetration of irrigants into the lateral canals.
