The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform

Ji-ye Zhu - One of the best experts on this subject based on the ideXlab platform.

  • Overexpression of Chaperonin Containing TCP1, subunit 3 predicts poor prognosis in hepatocellular carcinoma
    World journal of gastroenterology, 2015
    Co-Authors: Xiao Cui, Hu Zhiping, Peng-ji Gao, Ji-ye Zhu
    Abstract:

    AIM: To investigate the value of Chaperonin Containing TCP1, subunit 3 (CCT3) to predict the prognosis of patients with hepatocellular carcinoma (HCC) and determine its function in HCC progression. METHODS: CCT3 expression levels were examined in human non-cancerous liver tissues and a variety of HCC cell lines by quantitative real-time PCR and immunoblotting. CCT3 expression was suppressed by small interfering RNA. The effects of reducing CCT3 expression in HCC cells were tested. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay, cell counting experiment, cell cycle assay, apoptosis assay and invasion assay were employed to evaluate cell functions in vitro. Immunohistochemistry was performed on HCC specimens. In addition, CCT3 expression in HCC specimens was also assessed at the protein and mRNA level. Associations between clinicopathological characteristics and prognosis were analyzed, along with the possible mechanisms involved in CCT3’s function in HCC progression. RESULTS: The expression levels of CCT3 mRNA and protein were upregulated in HCC cell lines in contrast to adjacent non-cancerous tissues. Reducing CCT3 expression not only suppressed cell proliferation in cell counts, MTT assay, cell cycle assay and induced cell apoptosis (P < 0.05 vs negative control), but also inhibited the tumor cell invasion capacity in vitro (P < 0.01 vs negative control). Overexpression of CCT3 in the nuclei of cancer cells in HCC specimens (58 of 104 patients, 55.8%) was associated with poor prognosis in HCC patients (3-year survival rate, 55.5% vs 84.2%, P = 0.020) after hepatectomy. Mechanistic analyses showed that signal transducer and activator of transcription 3 (STAT3) activation was decreased even when stimulated by interleukin-6 after knocking down CCT3 in the HepG2 cell line. CONCLUSION: Overexpression of CCT3 in the nuclei of cancerous cells is associated with HCC progression. CCT3 may be a target that affects the activation of STAT3 in HCC.

  • overexpression of Chaperonin Containing TCP1 subunit 3 predicts poor prognosis in hepatocellular carcinoma
    World Journal of Gastroenterology, 2015
    Co-Authors: Xiao Cui, Peng-ji Gao, Ji-ye Zhu
    Abstract:

    Overexpression of Chaperonin Containing TCP1, subunit 3 predicts poor prognosis in hepatocellular carcinoma

  • Overexpression of Chaperonin Containing TCP1, subunit 3 predicts poor prognosis in hepatocellular carcinoma
    WORLD JOURNAL OF GASTROENTEROLOGY, 2015
    Co-Authors: Cui Xiao, Hu Zhi-ping, Li Zhao, Gao Peng-ji, Ji-ye Zhu
    Abstract:

    AIM: To investigate the value of Chaperonin Containing TCP1, subunit 3 (CCT3) to predict the prognosis of patients with hepatocellular carcinoma (HCC) and determine its function in HCC progression. METHODS: CCT3 expression levels were examined in human non-cancerous liver tissues and a variety of HCC cell lines by quantitative real-time PCR and immunoblotting. CCT3 expression was suppressed by small interfering RNA. The effects of reducing CCT3 expression in HCC cells were tested. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay, cell counting experiment, cell cycle assay, apoptosis assay and invasion assay were employed to evaluate cell functions in vitro. Immunohistochemistry was performed on HCC specimens. In addition, CCT3 expression in HCC specimens was also assessed at the protein and mRNA level. Associations between clinicopathological characteristics and prognosis were analyzed, along with the possible mechanisms involved in CCT3's function in HCC progression. RESULTS: The expression levels of CCT3 mRNA and protein were upregulated in HCC cell lines in contrast to adjacent non-cancerous tissues. Reducing CCT3 expression not only suppressed cell proliferation in cell counts, MTT assay, cell cycle assay and induced cell apoptosis (P < 0.05 vs negative control), but also inhibited the tumor cell invasion capacity in vitro (P < 0.01 vs negative control). Overexpression of CCT3 in the nuclei of cancer cells in HCC specimens (58 of 104 patients, 55.8%) was associated with poor prognosis in HCC patients (3-year survival rate, 55.5% vs 84.2%, P = 0.020) after hepatectomy. Mechanistic analyses showed that signal transducer and activator of transcription 3 (STAT3) activation was decreased even when stimulated by interleukin-6 after knocking down CCT3 in the HepG2 cell line. CONCLUSION: Overexpression of CCT3 in the nuclei of cancerous cells is associated with HCC progression. CCT3 may be a target that affects the activation of STAT3 in HCC.Beijing Key Laboratory Special Fund [Z141107004414042]SCI(E)PubMedARTICLEgandanwk@vip.sina.com288588-86042

Claudina Rodrigues-pousada - One of the best experts on this subject based on the ideXlab platform.

  • Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment
    Biochimica et biophysica acta, 1999
    Co-Authors: Célia Domingues, Helena Soares, Claudina Rodrigues-pousada, Luísa Cyrne
    Abstract:

    Abstract We report here the cloning and the characterization of the Tetrahymena pyriformis Chaperonin-Containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse Chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative p I of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells.

  • The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric Chaperonin complex.
    Biochemical Journal, 1997
    Co-Authors: Helena Soares, Luísa Cyrne, Cristina Casalou, Bruno Ehmann, Claudina Rodrigues-pousada
    Abstract:

    The sequence of a third member of the Tetrahymena pyriformis Chaperonin CCT (‘Chaperonin Containing TCP1’) subunit gene family is presented. This gene, designated TpCCTa, is the orthologue of the mouse Chaperonin gene TCP1}CCTa .T o characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on Cterminal sequences deduced from the primary sequences of the TpCCTa, TpCCTc and TpCCTg subunits. We have also used polyclonal antibodies produced against recombinant yeast CCTa and CCTb subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT Chaperonin

  • The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric Chaperonin complex.
    The Biochemical journal, 1997
    Co-Authors: Helena Soares, Luísa Cyrne, Cristina Casalou, Bruno Ehmann, Claudina Rodrigues-pousada
    Abstract:

    The sequence of a third member of the Tetrahymena pyriformis Chaperonin CCT ('Chaperonin Containing TCP1') subunit gene family is presented. This gene, designated TpCCT alpha, is the orthologue of the mouse Chaperonin gene TCP1/CCT alpha. To characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on C-terminal sequences deduced from the primary sequences of the TpCCT alpha, TpCCT gamma and TpCCT eta subunits. We have also used polyclonal antibodies produced against recombinant yeast CCT alpha and CCT beta subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT Chaperonin comprising at least seven distinct subunits. Three of these were assigned to specific TpCCT genes, whereas a fourth was recognized by the polyclonal antibody against yeast CCT beta, suggesting that this gene is also present in the ciliate. The CCT complex also contains other unidentified proteins that were recognized by the polyclonal antibody UM-1, raised against the putative ATP binding domain of the Chaperonin proteins. TpCCT alpha gene expression was shown in exponentially growing cells and cells regenerating their cilia for different periods to have a similar pattern to the previously identified genes TpCCT gamma and TpCCT eta, and also to tubulin genes.

Helena Soares - One of the best experts on this subject based on the ideXlab platform.

  • Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment
    Biochimica et biophysica acta, 1999
    Co-Authors: Célia Domingues, Helena Soares, Claudina Rodrigues-pousada, Luísa Cyrne
    Abstract:

    Abstract We report here the cloning and the characterization of the Tetrahymena pyriformis Chaperonin-Containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse Chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative p I of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells.

  • The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric Chaperonin complex.
    Biochemical Journal, 1997
    Co-Authors: Helena Soares, Luísa Cyrne, Cristina Casalou, Bruno Ehmann, Claudina Rodrigues-pousada
    Abstract:

    The sequence of a third member of the Tetrahymena pyriformis Chaperonin CCT (‘Chaperonin Containing TCP1’) subunit gene family is presented. This gene, designated TpCCTa, is the orthologue of the mouse Chaperonin gene TCP1}CCTa .T o characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on Cterminal sequences deduced from the primary sequences of the TpCCTa, TpCCTc and TpCCTg subunits. We have also used polyclonal antibodies produced against recombinant yeast CCTa and CCTb subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT Chaperonin

  • The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric Chaperonin complex.
    The Biochemical journal, 1997
    Co-Authors: Helena Soares, Luísa Cyrne, Cristina Casalou, Bruno Ehmann, Claudina Rodrigues-pousada
    Abstract:

    The sequence of a third member of the Tetrahymena pyriformis Chaperonin CCT ('Chaperonin Containing TCP1') subunit gene family is presented. This gene, designated TpCCT alpha, is the orthologue of the mouse Chaperonin gene TCP1/CCT alpha. To characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on C-terminal sequences deduced from the primary sequences of the TpCCT alpha, TpCCT gamma and TpCCT eta subunits. We have also used polyclonal antibodies produced against recombinant yeast CCT alpha and CCT beta subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT Chaperonin comprising at least seven distinct subunits. Three of these were assigned to specific TpCCT genes, whereas a fourth was recognized by the polyclonal antibody against yeast CCT beta, suggesting that this gene is also present in the ciliate. The CCT complex also contains other unidentified proteins that were recognized by the polyclonal antibody UM-1, raised against the putative ATP binding domain of the Chaperonin proteins. TpCCT alpha gene expression was shown in exponentially growing cells and cells regenerating their cilia for different periods to have a similar pattern to the previously identified genes TpCCT gamma and TpCCT eta, and also to tubulin genes.

Luísa Cyrne - One of the best experts on this subject based on the ideXlab platform.

  • Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment
    Biochimica et biophysica acta, 1999
    Co-Authors: Célia Domingues, Helena Soares, Claudina Rodrigues-pousada, Luísa Cyrne
    Abstract:

    Abstract We report here the cloning and the characterization of the Tetrahymena pyriformis Chaperonin-Containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse Chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative p I of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells.

  • The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric Chaperonin complex.
    Biochemical Journal, 1997
    Co-Authors: Helena Soares, Luísa Cyrne, Cristina Casalou, Bruno Ehmann, Claudina Rodrigues-pousada
    Abstract:

    The sequence of a third member of the Tetrahymena pyriformis Chaperonin CCT (‘Chaperonin Containing TCP1’) subunit gene family is presented. This gene, designated TpCCTa, is the orthologue of the mouse Chaperonin gene TCP1}CCTa .T o characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on Cterminal sequences deduced from the primary sequences of the TpCCTa, TpCCTc and TpCCTg subunits. We have also used polyclonal antibodies produced against recombinant yeast CCTa and CCTb subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT Chaperonin

  • The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric Chaperonin complex.
    The Biochemical journal, 1997
    Co-Authors: Helena Soares, Luísa Cyrne, Cristina Casalou, Bruno Ehmann, Claudina Rodrigues-pousada
    Abstract:

    The sequence of a third member of the Tetrahymena pyriformis Chaperonin CCT ('Chaperonin Containing TCP1') subunit gene family is presented. This gene, designated TpCCT alpha, is the orthologue of the mouse Chaperonin gene TCP1/CCT alpha. To characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on C-terminal sequences deduced from the primary sequences of the TpCCT alpha, TpCCT gamma and TpCCT eta subunits. We have also used polyclonal antibodies produced against recombinant yeast CCT alpha and CCT beta subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT Chaperonin comprising at least seven distinct subunits. Three of these were assigned to specific TpCCT genes, whereas a fourth was recognized by the polyclonal antibody against yeast CCT beta, suggesting that this gene is also present in the ciliate. The CCT complex also contains other unidentified proteins that were recognized by the polyclonal antibody UM-1, raised against the putative ATP binding domain of the Chaperonin proteins. TpCCT alpha gene expression was shown in exponentially growing cells and cells regenerating their cilia for different periods to have a similar pattern to the previously identified genes TpCCT gamma and TpCCT eta, and also to tubulin genes.

Xiao Cui - One of the best experts on this subject based on the ideXlab platform.

  • Overexpression of Chaperonin Containing TCP1, subunit 3 predicts poor prognosis in hepatocellular carcinoma
    World journal of gastroenterology, 2015
    Co-Authors: Xiao Cui, Hu Zhiping, Peng-ji Gao, Ji-ye Zhu
    Abstract:

    AIM: To investigate the value of Chaperonin Containing TCP1, subunit 3 (CCT3) to predict the prognosis of patients with hepatocellular carcinoma (HCC) and determine its function in HCC progression. METHODS: CCT3 expression levels were examined in human non-cancerous liver tissues and a variety of HCC cell lines by quantitative real-time PCR and immunoblotting. CCT3 expression was suppressed by small interfering RNA. The effects of reducing CCT3 expression in HCC cells were tested. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay, cell counting experiment, cell cycle assay, apoptosis assay and invasion assay were employed to evaluate cell functions in vitro. Immunohistochemistry was performed on HCC specimens. In addition, CCT3 expression in HCC specimens was also assessed at the protein and mRNA level. Associations between clinicopathological characteristics and prognosis were analyzed, along with the possible mechanisms involved in CCT3’s function in HCC progression. RESULTS: The expression levels of CCT3 mRNA and protein were upregulated in HCC cell lines in contrast to adjacent non-cancerous tissues. Reducing CCT3 expression not only suppressed cell proliferation in cell counts, MTT assay, cell cycle assay and induced cell apoptosis (P < 0.05 vs negative control), but also inhibited the tumor cell invasion capacity in vitro (P < 0.01 vs negative control). Overexpression of CCT3 in the nuclei of cancer cells in HCC specimens (58 of 104 patients, 55.8%) was associated with poor prognosis in HCC patients (3-year survival rate, 55.5% vs 84.2%, P = 0.020) after hepatectomy. Mechanistic analyses showed that signal transducer and activator of transcription 3 (STAT3) activation was decreased even when stimulated by interleukin-6 after knocking down CCT3 in the HepG2 cell line. CONCLUSION: Overexpression of CCT3 in the nuclei of cancerous cells is associated with HCC progression. CCT3 may be a target that affects the activation of STAT3 in HCC.

  • overexpression of Chaperonin Containing TCP1 subunit 3 predicts poor prognosis in hepatocellular carcinoma
    World Journal of Gastroenterology, 2015
    Co-Authors: Xiao Cui, Peng-ji Gao, Ji-ye Zhu
    Abstract:

    Overexpression of Chaperonin Containing TCP1, subunit 3 predicts poor prognosis in hepatocellular carcinoma