The Experts below are selected from a list of 201 Experts worldwide ranked by ideXlab platform

Ahmed Mohamed Abdelsalam - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Fahd M. Alsharif, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Ahmed Mohamed Abdelsalam
    Abstract:

    The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

Swapan K. Ghosh - One of the best experts on this subject based on the ideXlab platform.

  • Electronegativity, hardness, and a semiempirical density functional theory of Chemical Binding
    International Journal of Quantum Chemistry, 1994
    Co-Authors: Swapan K. Ghosh
    Abstract:

    Chemical Binding is modeled through interatomic charge transfer and accumulation of electron density at the bond center using the concepts of electronegativity and hardness parameters defined for the bond region. The generalized electronegativity and hardness parameters for the up- and down-spin electrons are also defined within the framework of spin-polarized density functional theory, leading to the formulation of covalent Binding in molecules in terms of a two-way flow of unpaired electrons between the atoms. The associated energy changes corresponding to these descriptions are shown to provide quite accurate predictions of bond energies for simple heteronuclear diatomic molecules. © 1994 John Wiley & Sons, Inc.

  • A New Simple Density Functional Approach to Chemical Binding
    The Journal of Physical Chemistry, 1994
    Co-Authors: Tapan K. Ghanty, Swapan K. Ghosh
    Abstract:

    A new simple density functional approach to Chemical Binding in molecules is developed by representing the electron density reorganization of the constituent atoms in terms of the atomic charges and dipoles. The potential and capacity parameters for the atomic dipole are introduced, and a new equation for Binding energy is derived. The calculated numerical results on the bond energies of a number of homo- and heteronuclear diatomics are shown to agree quite well with available data

  • A new electronegativity-based approach to Chemical Binding
    Journal of the Chemical Society Chemical Communications, 1992
    Co-Authors: Tapan K. Ghanty, Swapan K. Ghosh
    Abstract:

    A new theory of Chemical Binding modelled through the accumulation of electron density at the bond centre using the concepts of bond electronegativity and bond hardness is reported and shown to provide accurate prediction of bond energies for a number of diatomic and simple polyatomic (ABn) molecules.

  • Electronegativity, hardness, and Chemical Binding in simple molecular systems
    Inorganic Chemistry, 1992
    Co-Authors: Tapan K. Ghanty, Swapan K. Ghosh
    Abstract:

    A new electronegativity-based apprach to Chemical Binding is proposed where the covalent Binding is formulated in terms of the accumulation of electron density at the bond center using the concepts of bond electronegativity and bond hardness. In an AB n type molecule, the covalent contribution to the single A-B bond energy is shown to be given by a simple expression in terms of the A-A and B-B bond energies. For heteronuclear diatomic molecules, this reduces to an average of the geometric and arithmetic means of the bond energies of corresponding homonuclear diatomics

Gamal M. Zayed - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Fahd M. Alsharif, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Ahmed Mohamed Abdelsalam
    Abstract:

    The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

Montaser Sh. A. Shaykoon - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Fahd M. Alsharif, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Ahmed Mohamed Abdelsalam
    Abstract:

    The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

Hatem A. Sarhan - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Fahd M. Alsharif, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Ahmed Mohamed Abdelsalam
    Abstract:

    The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

  • Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
    Pharmaceutical Research, 2018
    Co-Authors: Gamal M. Zayed, Islam Kamal, Wael A. Abdelhafez, Mohamed A. Amin, Montaser Sh. A. Shaykoon, Hatem A. Sarhan, Fahd M. Alsharif, Ahmed Mohamed Abdelsalam
    Abstract:

    Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the Chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the Chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the Chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical Binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.