The Experts below are selected from a list of 155598 Experts worldwide ranked by ideXlab platform

Jeanloup Faulon - One of the best experts on this subject based on the ideXlab platform.

  • plug and play metabolic transducers expand the Chemical Detection space of cell free biosensors
    Nature Communications, 2019
    Co-Authors: Peter L Voyvodic, Amir Pandi, Mathilde Koch, Ismael Conejero, Emmanuel Valjent, Philippe Courtet, Eric Renard, Jeanloup Faulon
    Abstract:

    Cell-free transcription–translation systems have great potential for biosensing, yet the range of detectable Chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. In addition, they are capable of functioning in a variety of complex media, including commercial beverages and human urine, in which they can be used to detect clinically relevant concentrations of small molecules. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.

  • plug and play metabolic transducers expand the Chemical Detection space of cell free biosensors
    bioRxiv, 2018
    Co-Authors: Peter L Voyvodic, Amir Pandi, Mathilde Koch, Jeanloup Faulon, Jerome Bonnet
    Abstract:

    Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable Chemicals is limited. Here we provide a framework to expand the range of molecules detectable by cell-free biosensors by combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors are highly-sensitive and have a fast response and high-dynamic range. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.

Taeyun Kwon - One of the best experts on this subject based on the ideXlab platform.

  • nanomechanical resonators and their applications in biological Chemical Detection nanomechanics principles
    Physics Reports, 2011
    Co-Authors: Kilho Eom, Harold S Park, Dae Sung Yoon, Taeyun Kwon
    Abstract:

    Abstract Recent advances in nanotechnology have led to the development of nano-electro-mechanical systems (NEMS) such as nanomechanical resonators, which have recently received significant attention from the scientific community. This is not only due to their capability of label-free Detection of bio/Chemical molecules at single-molecule (or atomic) resolution for future applications such as the early diagnosis of diseases like cancer, but also due to their unprecedented ability to detect physical quantities such as molecular weight, elastic stiffness, surface stress, and surface elastic stiffness for adsorbed molecules on the surface. Most experimental works on resonator-based molecular Detection have been based on the principle that molecular adsorption onto a resonator surface increases the effective mass, and consequently decreases the resonant frequencies of the nanomechanical resonator. However, this principle is insufficient to provide fundamental insights into resonator-based molecular Detection at the nanoscale; this is due to recently proposed novel nanoscale Detection principles including various effects such as surface effects, nonlinear oscillations, coupled resonance, and stiffness effects. Furthermore, these effects have only recently been incorporated into existing physical models for resonators, and therefore the universal physical principles governing nanoresonator-based Detection have not been completely described. Therefore, our objective in this review is to overview the current attempts to understand the underlying mechanisms in nanoresonator-based Detection using physical models coupled to computational simulations and/or experiments. Specifically, we will focus on issues of special relevance to the dynamic behavior of nanoresonators and their applications in biological/Chemical Detection: the resonance behavior of micro/nanoresonators; resonator-based Chemical/biological Detection; physical models of various nanoresonators such as nanowires, carbon nanotubes, and graphene. We pay particular attention to experimental and computational approaches that have been useful in elucidating the mechanisms underlying the dynamic behavior of resonators across multiple and disparate spatial/length scales, and the resulting insight into resonator-based Detection that has been obtained. We additionally provide extensive discussion regarding potentially fruitful future research directions coupling experiments and simulations in order to develop a fundamental understanding of the basic physical principles that govern NEMS and NEMS-based sensing and Detection applications.

  • nanomechanical resonators and their applications in biological Chemical Detection nanomechanics principles
    arXiv: Mesoscale and Nanoscale Physics, 2011
    Co-Authors: Kilho Eom, Harold S Park, Dae Sung Yoon, Taeyun Kwon
    Abstract:

    Recent advances in nanotechnology have led to the development of nano-electro-mechanical systems (NEMS) such as nanomechanical resonators, which have recently received significant attention from the scientific community. This has not only been for their capability for the label-free Detection of bio/Chemical-molecules at single-molecule (or atomic) resolution for future applications such as the early diagnostics of diseases such as cancer, but also for their unprecedented ability to detect physical quantities such as molecular weight, elastic stiffness, surface stress, and surface elastic stiffness for adsorbed molecules on the surface. Most experimental works on resonator-based molecular Detection have been based on the principle that molecular adsorption onto a resonator surface increases the effective mass, and consequently decreases the resonant frequencies of the nanomechanical resonator. However, this principle is insufficient to provide fundamental insights into resonator-based molecular Detection at the nanoscale; this is due to recently proposed novel nanoscale Detection principles including various effects such as surface effects, nonlinear oscillations, coupled resonance, and stiffness effects. Therefore, our objective in this review is to overview the current attempts to understand the underlying mechanisms in nanoresonator-based Detection using physical models coupled to computational simulations and/or experiments. Specifically, we will focus on issues of special relevance to the dynamic behavior of nanoresonators and their applications in biological/Chemical Detection. We additionally provide extensive discussion regarding potentially fruitful future research directions coupling experiments and simulations in order to develop a fundamental understanding of the basic physical principles that govern NEMS and NEMS-based sensing applications.

Ismael Conejero - One of the best experts on this subject based on the ideXlab platform.

  • plug and play metabolic transducers expand the Chemical Detection space of cell free biosensors
    Nature Communications, 2019
    Co-Authors: Peter L Voyvodic, Amir Pandi, Mathilde Koch, Ismael Conejero, Emmanuel Valjent, Philippe Courtet, Eric Renard, Jeanloup Faulon
    Abstract:

    Cell-free transcription–translation systems have great potential for biosensing, yet the range of detectable Chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. In addition, they are capable of functioning in a variety of complex media, including commercial beverages and human urine, in which they can be used to detect clinically relevant concentrations of small molecules. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.

Benjamin Basso - One of the best experts on this subject based on the ideXlab platform.

  • Chemical Detection triggers honey bee defense against a destructive parasitic threat
    Nature Chemical Biology, 2021
    Co-Authors: Fanny Mondet, Solene Blanchard, Nicolas Barthes, Dominique Beslay, Celia Bordier, Guy Costagliola, Maxime R. Hervé, Benoit Lapeyre, Seo Hyun Kim, Benjamin Basso
    Abstract:

    Invasive species events related to globalization are increasing, resulting in parasitic outbreaks. Understanding of host defense mechanisms is needed to predict and mitigate against the consequences of parasite invasion. Using the honey bee Apis mellifera and the mite Varroa destructor, as a host–parasite model, we provide a comprehensive study of a mechanism of parasite Detection that triggers a behavioral defense associated with social immunity. Six Varroa-parasitization-specific (VPS) compounds are identified that trigger Varroa-sensitive hygiene (VSH, bees’ key defense against Varroa sp.), enable the selective recognition of a parasitized brood and induce responses that mimic intrinsic VSH activity in bee colonies. We also show that individuals engaged in VSH exhibit a unique ability to discriminate VPS compounds from healthy brood signals. These findings enhance our understanding of a critical mechanism of host defense against parasites, and have the potential to apply the integration of pest management in the beekeeping sector.

Kilho Eom - One of the best experts on this subject based on the ideXlab platform.

  • nanomechanical resonators and their applications in biological Chemical Detection nanomechanics principles
    Physics Reports, 2011
    Co-Authors: Kilho Eom, Harold S Park, Dae Sung Yoon, Taeyun Kwon
    Abstract:

    Abstract Recent advances in nanotechnology have led to the development of nano-electro-mechanical systems (NEMS) such as nanomechanical resonators, which have recently received significant attention from the scientific community. This is not only due to their capability of label-free Detection of bio/Chemical molecules at single-molecule (or atomic) resolution for future applications such as the early diagnosis of diseases like cancer, but also due to their unprecedented ability to detect physical quantities such as molecular weight, elastic stiffness, surface stress, and surface elastic stiffness for adsorbed molecules on the surface. Most experimental works on resonator-based molecular Detection have been based on the principle that molecular adsorption onto a resonator surface increases the effective mass, and consequently decreases the resonant frequencies of the nanomechanical resonator. However, this principle is insufficient to provide fundamental insights into resonator-based molecular Detection at the nanoscale; this is due to recently proposed novel nanoscale Detection principles including various effects such as surface effects, nonlinear oscillations, coupled resonance, and stiffness effects. Furthermore, these effects have only recently been incorporated into existing physical models for resonators, and therefore the universal physical principles governing nanoresonator-based Detection have not been completely described. Therefore, our objective in this review is to overview the current attempts to understand the underlying mechanisms in nanoresonator-based Detection using physical models coupled to computational simulations and/or experiments. Specifically, we will focus on issues of special relevance to the dynamic behavior of nanoresonators and their applications in biological/Chemical Detection: the resonance behavior of micro/nanoresonators; resonator-based Chemical/biological Detection; physical models of various nanoresonators such as nanowires, carbon nanotubes, and graphene. We pay particular attention to experimental and computational approaches that have been useful in elucidating the mechanisms underlying the dynamic behavior of resonators across multiple and disparate spatial/length scales, and the resulting insight into resonator-based Detection that has been obtained. We additionally provide extensive discussion regarding potentially fruitful future research directions coupling experiments and simulations in order to develop a fundamental understanding of the basic physical principles that govern NEMS and NEMS-based sensing and Detection applications.

  • nanomechanical resonators and their applications in biological Chemical Detection nanomechanics principles
    arXiv: Mesoscale and Nanoscale Physics, 2011
    Co-Authors: Kilho Eom, Harold S Park, Dae Sung Yoon, Taeyun Kwon
    Abstract:

    Recent advances in nanotechnology have led to the development of nano-electro-mechanical systems (NEMS) such as nanomechanical resonators, which have recently received significant attention from the scientific community. This has not only been for their capability for the label-free Detection of bio/Chemical-molecules at single-molecule (or atomic) resolution for future applications such as the early diagnostics of diseases such as cancer, but also for their unprecedented ability to detect physical quantities such as molecular weight, elastic stiffness, surface stress, and surface elastic stiffness for adsorbed molecules on the surface. Most experimental works on resonator-based molecular Detection have been based on the principle that molecular adsorption onto a resonator surface increases the effective mass, and consequently decreases the resonant frequencies of the nanomechanical resonator. However, this principle is insufficient to provide fundamental insights into resonator-based molecular Detection at the nanoscale; this is due to recently proposed novel nanoscale Detection principles including various effects such as surface effects, nonlinear oscillations, coupled resonance, and stiffness effects. Therefore, our objective in this review is to overview the current attempts to understand the underlying mechanisms in nanoresonator-based Detection using physical models coupled to computational simulations and/or experiments. Specifically, we will focus on issues of special relevance to the dynamic behavior of nanoresonators and their applications in biological/Chemical Detection. We additionally provide extensive discussion regarding potentially fruitful future research directions coupling experiments and simulations in order to develop a fundamental understanding of the basic physical principles that govern NEMS and NEMS-based sensing applications.