The Experts below are selected from a list of 6690 Experts worldwide ranked by ideXlab platform
Mitsuo Miyazawa - One of the best experts on this subject based on the ideXlab platform.
-
suppression of Chemical Mutagen induced sos response by allylbenzen from asiasarum heterotropoides in the salmonella typhimurium ta1535 psk1002 umu test
Natural Product Research, 2005Co-Authors: Mitsuo Miyazawa, Go KohnoAbstract:A methanol extract from Asiasarum heterotropoides showed a suppressive effect of the SOS-including activity on the Mutagen 2-amino-3,4-dimethylimidazo[4,5-f ]quinoline (MeIQ) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with chloroform, butanol, and water. The chloroform fraction showed a suppressive effect. Suppressive compounds in the chloroform fraction were isolated by silica gel column chromatography and identified as methyleugenol (1), elemicin (2), and γ-asaron (3) by GC/MS, IR, and 1H- and 13C-NMR spectroscopy. These compounds suppressed the MeIQ-induced SOS response in the umu test. Gene expression was suppressed 52.2, 61.8, and 71.6% at a concentration of 0.1 mM, respectively. The ID50 values (50% inhibition dose) of these compounds were 0.080, 0.028, and 0.013 mM, respectively. On the other hand, Compounds 1–3 showed weak suppressive effect of the SOS-inducing activity on activated MeIQ. These results indicate that the inhibition of the SOS-induci...
-
suppression of Chemical Mutagen induced sos response by alkylphenols from clove syzygium aromaticum in the salmonella typhimurium ta1535 psk1002 umu test
Journal of Agricultural and Food Chemistry, 2001Co-Authors: Mitsuo Miyazawa, Masayoshi HisamaAbstract:A methanol extract from clove (Syzygium aromaticum) showed a suppressive effect of the SOS-inducing activity on the Mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with hexane, dichloromethane, ethyl acetate, butanol, and water. The hexane fraction showed a suppressive effect. Suppressive compounds in the hexane fraction were isolated by silica gel column chromatography and identified as trans-isoeugenol (1) and eugenol (2) by GC, GC-MS, IR, and 1H and 13C NMR spectroscopy. Compounds 1 and 2 suppressed the furylfuramide-induced SOS response in the umu test. Compounds 1 and 2 suppressed 42.3 and 29.9% of the SOS-inducing activity at a concentration of 0.60 μmol/mL. These compounds were assayed with other Mutagens, 4-nitroquinolin 1-oxide (4NQO) and N-methyl-N‘-nitro-N-nitrosoguanidine (MNNG). In addition, compounds 1 and 2 were assayed with aflatoxin B1 (AfB1) and 3-amino-1,4-dimethyl-5H-pyrido[4,...
Go Kohno - One of the best experts on this subject based on the ideXlab platform.
-
suppression of Chemical Mutagen induced sos response by allylbenzen from asiasarum heterotropoides in the salmonella typhimurium ta1535 psk1002 umu test
Natural Product Research, 2005Co-Authors: Mitsuo Miyazawa, Go KohnoAbstract:A methanol extract from Asiasarum heterotropoides showed a suppressive effect of the SOS-including activity on the Mutagen 2-amino-3,4-dimethylimidazo[4,5-f ]quinoline (MeIQ) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with chloroform, butanol, and water. The chloroform fraction showed a suppressive effect. Suppressive compounds in the chloroform fraction were isolated by silica gel column chromatography and identified as methyleugenol (1), elemicin (2), and γ-asaron (3) by GC/MS, IR, and 1H- and 13C-NMR spectroscopy. These compounds suppressed the MeIQ-induced SOS response in the umu test. Gene expression was suppressed 52.2, 61.8, and 71.6% at a concentration of 0.1 mM, respectively. The ID50 values (50% inhibition dose) of these compounds were 0.080, 0.028, and 0.013 mM, respectively. On the other hand, Compounds 1–3 showed weak suppressive effect of the SOS-inducing activity on activated MeIQ. These results indicate that the inhibition of the SOS-induci...
Masayoshi Hisama - One of the best experts on this subject based on the ideXlab platform.
-
suppression of Chemical Mutagen induced sos response by alkylphenols from clove syzygium aromaticum in the salmonella typhimurium ta1535 psk1002 umu test
Journal of Agricultural and Food Chemistry, 2001Co-Authors: Mitsuo Miyazawa, Masayoshi HisamaAbstract:A methanol extract from clove (Syzygium aromaticum) showed a suppressive effect of the SOS-inducing activity on the Mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with hexane, dichloromethane, ethyl acetate, butanol, and water. The hexane fraction showed a suppressive effect. Suppressive compounds in the hexane fraction were isolated by silica gel column chromatography and identified as trans-isoeugenol (1) and eugenol (2) by GC, GC-MS, IR, and 1H and 13C NMR spectroscopy. Compounds 1 and 2 suppressed the furylfuramide-induced SOS response in the umu test. Compounds 1 and 2 suppressed 42.3 and 29.9% of the SOS-inducing activity at a concentration of 0.60 μmol/mL. These compounds were assayed with other Mutagens, 4-nitroquinolin 1-oxide (4NQO) and N-methyl-N‘-nitro-N-nitrosoguanidine (MNNG). In addition, compounds 1 and 2 were assayed with aflatoxin B1 (AfB1) and 3-amino-1,4-dimethyl-5H-pyrido[4,...
Lu Rui - One of the best experts on this subject based on the ideXlab platform.
-
Effects of Chemical Mutagen and ~(60)Co Treatments on Microspore Culture of Barley under Low-Nitrogen Stress
Plant Physiology, 2013Co-Authors: Lu RuiAbstract:To investigate the effects of Chemical Mutagens and 60Co on callus yield and green plantlet production through microspore culture under low-nitrogen stress, we used the barley(Hordeum vulgare) variety ‘Hua 30' as the tested material, used two Chemical Mutagens, ethylmethylsulfone(EMS) and pingyangmycin(PYM), with different concentrations to treat the microspores, and used 60Co with different doses to treat young spikes and seeds respectively. The results showed that EMS treatment of microspores or 60Co treatment of seeds were much better than PYM treatment of microspores or 60Co treatment of young spikes for callus induction and green plantlet differentiation.
-
effects of Chemical Mutagen and 60 co treatments on microspore culture of barley under low nitrogen stress
Plant Physiology, 2013Co-Authors: Lu RuiAbstract:To investigate the effects of Chemical Mutagens and 60Co on callus yield and green plantlet production through microspore culture under low-nitrogen stress, we used the barley(Hordeum vulgare) variety ‘Hua 30' as the tested material, used two Chemical Mutagens, ethylmethylsulfone(EMS) and pingyangmycin(PYM), with different concentrations to treat the microspores, and used 60Co with different doses to treat young spikes and seeds respectively. The results showed that EMS treatment of microspores or 60Co treatment of seeds were much better than PYM treatment of microspores or 60Co treatment of young spikes for callus induction and green plantlet differentiation.
Hiroshi Honda - One of the best experts on this subject based on the ideXlab platform.
-
a genome wide mutation analysis method enabling high throughput identification of Chemical Mutagen signatures
Scientific Reports, 2018Co-Authors: Shoji Matsumura, Yurika Fujita, Masayuki Yamane, Osamu Morita, Hiroshi HondaAbstract:Trinucleotide mutational signatures extracted from cancer genomes provide clues useful in understanding the roles of Mutagens and Mutagenic mechanisms in cancer development. The lack of a simple method for genome-wide analysis of alterations induced by Mutagens hampers the identification of trinucleotide signatures of Mutagen exposure and evaluation of their relationships with human cancers. Here, we describe a novel approach to facilitate analysis of Chemically induced mutations in bacterial cells by detection of increased frequencies of base substitutions after Mutagen exposure, using paired-end overlapping next-generation sequencing. DNA samples from Salmonella typhimurium strain TA100, exposed to three alkylating agents, ethylnitrosourea (ENU), methylnitrosourea (MNU), and ethyl methansulphonate (EMS), were analysed. The G:C > A:T mutation frequency was increased in all samples, whereas A:T base pair substitution frequencies were increased specifically in samples exposed to ENU, consistent with previous reports. Mutation patterns in the context of 96 possible trinucleotide formats in these samples exhibited a sharp peak corresponding to an NpCpY consensus sequence, which is similar to the mutational signature of alkylating agents in human cancer. These results indicate that our approach can be useful in facilitating the understanding of mechanisms underlying Chemical Mutagenicity and for identification of unknown causal Mutagens in human cancer.