The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Masaki Torimura - One of the best experts on this subject based on the ideXlab platform.
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long non coding rnas as surrogate indicators for Chemical Stress responses in human induced pluripotent stem cells
PLOS ONE, 2014Co-Authors: Hidenori Tani, Yasuko Onuma, Masaki TorimuraAbstract:In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular Stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified six novel lncRNAs (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630, LINC00152, and LINC0541471_v2) that respond to model Chemical Stresses (cycloheximide, hydrogen peroxide, cadmium, or arsenic) in hiPSCs. Our results indicated that the lncRNAs responded to general and specific Chemical Stresses. Compared with typical mRNAs such as p53-related mRNAs, the lncRNAs highly and rapidly responded to Chemical Stresses. We propose that these lncRNAs have the potential to be surrogate indicators of Chemical Stress responses in hiPSCs.
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Identification of short-lived long non-coding RNAs as surrogate indicators for Chemical Stress response
Biochemical and Biophysical Research Communications, 2013Co-Authors: Hidenori Tani, Masaki TorimuraAbstract:Abiotic and biotic Stressors in human cells are often a result of sudden and/or frequent changes in environmental factors. The molecular response to Stress involves elaborate modulation of gene expression and is of homeostatic, ecological, and evolutionary importance. Although attention has primarily focused on signaling pathways and protein networks, long non-coding RNAs (ncRNAs) are increasingly involved in the molecular mechanisms associated with responses to cellular Stresses. We identified six novel short-lived long ncRNAs (MIR22HG, GABPB-AS1, LINC00152, IDI2-AS1, SNHG15, and FLJ33630) that responded to Chemical Stressors (cisplatin, cycloheximide, and mercury (II) oxide) in HeLa Tet-off cells. Our results indicate that short-lived long ncRNAs respond to general and specific Chemical Stressors. The expression levels of the short-lived long ncRNAs were elevated because of prolonged decay rates in response to Chemical Stressors and interruption of RNA degradation pathways. We propose that these long ncRNAs have the potential to be surrogate indicators of cellular Stress responses.
Hidenori Tani - One of the best experts on this subject based on the ideXlab platform.
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Short-lived long noncoding RNAs as surrogate indicators for Chemical Stress in HepG2 cells and their degradation by nuclear RNases.
Scientific Reports, 2019Co-Authors: Hidenori Tani, Ayaka Numajiri, Motohide Aoki, Tomonari Umemura, Tetsuya NakazatoAbstract:Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts >200 nucleotides in length that have been shown to play important roles in various biological processes. The mechanisms underlying the induction of lncRNA expression by Chemical exposure remain to be determined. We identified a novel class of short-lived lncRNAs with half-lives (t1/2) ≤4 hours in human HeLa Tet-off cells, which have been suggested to express many lncRNAs with regulatory functions. As they may affect various human biological processes, short-lived lncRNAs may be useful indicators of the degree of Stress on Chemical exposure. In the present study, we identified four short-lived lncRNAs, designated as OIP5-AS1, FLJ46906, LINC01137, and GABPB1-AS1, which showed significantly upregulated expression following exposure to hydrogen peroxide (oxidative Stress), mercury II chloride (heavy metal Stress), and etoposide (DNA damage Stress) in human HepG2 cells. These lncRNAs may be useful indicators of Chemical Stress responses. The levels of these lncRNAs in the cells were increased because of Chemical Stress-induced prolongation of their decay. These lncRNAs were degraded by nuclear RNases, which are components of the exosome and XRN2, and Chemical exposure inhibited the RNase activities within the cells.
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long non coding rnas as surrogate indicators for Chemical Stress responses in human induced pluripotent stem cells
PLOS ONE, 2014Co-Authors: Hidenori Tani, Yasuko Onuma, Masaki TorimuraAbstract:In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular Stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified six novel lncRNAs (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630, LINC00152, and LINC0541471_v2) that respond to model Chemical Stresses (cycloheximide, hydrogen peroxide, cadmium, or arsenic) in hiPSCs. Our results indicated that the lncRNAs responded to general and specific Chemical Stresses. Compared with typical mRNAs such as p53-related mRNAs, the lncRNAs highly and rapidly responded to Chemical Stresses. We propose that these lncRNAs have the potential to be surrogate indicators of Chemical Stress responses in hiPSCs.
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Identification of short-lived long non-coding RNAs as surrogate indicators for Chemical Stress response
Biochemical and Biophysical Research Communications, 2013Co-Authors: Hidenori Tani, Masaki TorimuraAbstract:Abiotic and biotic Stressors in human cells are often a result of sudden and/or frequent changes in environmental factors. The molecular response to Stress involves elaborate modulation of gene expression and is of homeostatic, ecological, and evolutionary importance. Although attention has primarily focused on signaling pathways and protein networks, long non-coding RNAs (ncRNAs) are increasingly involved in the molecular mechanisms associated with responses to cellular Stresses. We identified six novel short-lived long ncRNAs (MIR22HG, GABPB-AS1, LINC00152, IDI2-AS1, SNHG15, and FLJ33630) that responded to Chemical Stressors (cisplatin, cycloheximide, and mercury (II) oxide) in HeLa Tet-off cells. Our results indicate that short-lived long ncRNAs respond to general and specific Chemical Stressors. The expression levels of the short-lived long ncRNAs were elevated because of prolonged decay rates in response to Chemical Stressors and interruption of RNA degradation pathways. We propose that these long ncRNAs have the potential to be surrogate indicators of cellular Stress responses.
Jens C Streibig - One of the best experts on this subject based on the ideXlab platform.
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Chemical Stress can increase crop yield
Field Crops Research, 2009Co-Authors: Nina Cedergreen, Claus Felby, John R Porter, Jens C StreibigAbstract:Abstract Low dose Chemical Stress has been shown to increase plant vegetative growth, though not all Chemicals induce the response. Glyphosate is the most widely used herbicide by volume and treated area. At low doses, it can increase growth in a variety of species. Here we show that a glyphosate-induced growth increase can be transformed into an increase in crop yield, if applied at the right time. Glyphosate, in the dose range of 2.5–20 g a.e. ha −1 , corresponding to less than 1% of the rate normally used for weed control in the field, increased grain yield of barley by 12–15% when applied at the time of grain filling. Straw yield and the quality of the grains in terms of nitrogen and starch content were not affected by the treatment. The physiological mechanism behind this counter intuitive increase in growth is still unknown, as are possible adverse effects. It is, however, evident that understanding the physiological processes behind Chemically induced growth increases in plants holds the promise of improving food yield.
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Chemical Stress can increase crop yield
Field Crops Research, 2009Co-Authors: Nina Cedergreen, Claus Felby, Jens C StreibigAbstract:Low dose Chemical Stress has been shown to increase plant vegetative growth, though not all Chemicals induce the response. Glyphosate is the most widely used herbicide by volume and treated area. At low doses, it can increase growth in a variety of species. Here we show that a glyphosate-induced growth increase can be transformed into an increase in crop yield, if applied at the right time. Glyphosate, in the dose range of 2.5-20 g a.e. ha-1, corresponding to less than 1% of the rate normally used for weed control in the field, increased grain yield of barley by 12-15% when applied at the time of grain filling. Straw yield and the quality of the grains in terms of nitrogen and starch content were not affected by the treatment. The physiological mechanism behind this counter intuitive increase in growth is still unknown, as are possible adverse effects. It is, however, evident that understanding the physiological processes behind Chemically induced growth increases in plants holds the promise of improving food yield. © 2009 Elsevier B.V. All rights reserved.
Jorg Rombke - One of the best experts on this subject based on the ideXlab platform.
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interactive effects of pyrimethanil soil moisture and temperature on folsomia candida and sinella curviseta collembola
Applied Soil Ecology, 2014Co-Authors: Cornelia Bandow, Nora Karau, Jorg RombkeAbstract:Abstract The aim of the presented investigation was to study effects resulting from specific aspects of climate change and Chemical Stress (individually or in interaction) on soil organisms. In detail, the interaction of different temperatures (20 °C and 26 °C) and soil moisture levels (30%, 50% and 70% of the water holding capacity) were examined in combination with the fungicide pyrimethanil on the reproduction of two Collembola species (Folsomia candida and Sinella curviseta). Testing was based on the standard collembolan reproduction test (OECD-Guideline 232), following an ECX design. Low soil moisture led to a significant reduction of the juveniles in the control groups in contrast to medium or high soil moisture. Furthermore, the results showed a significant influence of both climatic factors on the toxicity of the fungicide. In general, both species reacted more sensitive when exposure was conducted in dry soil or at enhanced temperature.
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enchytraeids as indicator organisms for Chemical Stress in terrestrial ecosystems
Ecotoxicology and Environmental Safety, 2001Co-Authors: Wim Didden, Jorg RombkeAbstract:This review article surveys the available data on enchytraeid sensitivity toward Chemical Stress, and the effects of Chemical Stress on enchytraeid communities in terrestrial ecosystems. The factors affecting bioavailability of Stressors to enchytraeids and the nature of direct and indirect effects that may occur are discussed, and the importance of laboratory tests and field studies for the evaluation of toxic effects is underlined. The existing data on specific types of Stressors such as pesticides, metals, fertilizers, and industrial and domestic Chemicals often show clear responses of enchytraeids, and in many cases interspecific differences in sensitivity are recorded. It is concluded that, although in several fields additional data are required, there are good perspectives for the use of enchytraeids as indicator organisms.
A M M Goncalves - One of the best experts on this subject based on the ideXlab platform.
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fatty acid profiling as bioindicator of Chemical Stress in marine organisms a review
Ecological Indicators, 2016Co-Authors: Valentina Filimonova, Fernando Goncalves, Joao Carlos Marques, Marleen De Troch, A M M GoncalvesAbstract:Abstract The development of industrial, anthropogenic, and agricultural activities is the main factor leading to contaminants’ increasing in marine ecosystems. Contaminants include the great variety of pesticides and heavy metal pollutants. One of the major environmental concerns about herbicides and heavy metals contamination is their bioaccumulation in the ecosystem's primary producers and its subsequent propagation through the trophic chain. Over the last decades, the use of bioChemical markers considerably contributed to the evaluation of contamination hazards. The fatty acid composition proved to be a good bioindicator to assess contamination levels. This paper provides a review of current knowledge on the fatty acids response in marine species after exposure to the Chemical Stressors including organic and inorganic pollutants, mainly pesticides and heavy metals. This review underlines the consistent directional trends in changes of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids, and summarizes the mechanisms of action leading to their alteration and possible consequences of these changes to marine species from different trophic levels.