The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Oleg Melnyk - One of the best experts on this subject based on the ideXlab platform.
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Protein Chemical Synthesis in medicinal chemistry
Journal of Medicinal Chemistry, 2020Co-Authors: Vangelis Agouridas, Ouafâa El Mahdi, Oleg MelnykAbstract:Although the majority of proteins used for biomedical research are produced using living systems such as bacteria, biological means for producing proteins can be advantageously complemented by protein semiSynthesis or total Chemical Synthesis. The latter approach is particularly useful when the proteins to be produced are toxic for the expression system or show unusual features that cannot be easily programmed in living organisms. The aim of this perspective article is to provide a wide overview of the use of protein Chemical Synthesis in medicinal chemistry with a special focus on the production of side-chain or backbone-modified proteins.
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A statistical view of protein Chemical Synthesis using NCL and extended methodologies.
Bioorganic & medicinal chemistry, 2017Co-Authors: Vangelis Agouridas, Ouafâa El Mahdi, Marine Cargoët, Oleg MelnykAbstract:Native Chemical ligation and extended methodologies are the most popular chemoselective reactions for protein Chemical Synthesis. Their combination with desulfurization techniques can give access to small or challenging proteins that are exploited in a large variety of research areas. In this report, we have conducted a statistical review of their use for protein Chemical Synthesis in order to provide a flavor of the recent trends and identify the most popular Chemical tools used by protein chemists. To this end, a protein Chemical Synthesis (PCS) database (http://pcs-db.fr) was created by collecting a set of relevant data from more than 450 publications covering the period 1994-2017. A preliminary account of what this database tells us is presented in this report.
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Total Chemical Synthesis of SUMO proteins
Tetrahedron Letters, 2016Co-Authors: Oleg Melnyk, Jérôme VicogneAbstract:Abstract The total Chemical Synthesis of proteins is a useful alternative to living systems for accessing small proteins. Significant progresses have been made these recent years in the design of novel chemoselective amide bond ligation chemistries that can be integrated in efficient peptide segment assembly strategies. These methods are used to tackle challenging protein targets that are not easily attainable using classical recombinant techniques. This digest focuses on the latest advances in the total Chemical Synthesis of small ubiquitin-like modifier (SUMO) proteins and SUMO peptide protein conjugates.
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Solid phase protein Chemical Synthesis.
Topics in current chemistry, 2014Co-Authors: Laurent Raibaut, Ouafâa El Mahdi, Oleg MelnykAbstract:The Chemical Synthesis of peptides or small proteins is often an important step in many research projects and has stimulated the development of numerous Chemical methodologies. The aim of this review is to give a substantial overview of the solid phase methods developed for the production or purification of polypeptides. The solid phase peptide Synthesis (SPPS) technique has facilitated considerably the access to short peptides (
Masanobu Uchiyama - One of the best experts on this subject based on the ideXlab platform.
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Room-temperature Chemical Synthesis of C_2
Nature Communications, 2020Co-Authors: Kazunori Miyamoto, Shodai Narita, Yui Masumoto, Takahiro Hashishin, Taisei Osawa, Mutsumi Kimura, Masahito Ochiai, Masanobu UchiyamaAbstract:Diatomic carbon (C_2) is historically an elusive Chemical species. It has long been believed that the generation of C_2 requires extremely high physical energy, such as an electric carbon arc or multiple photon excitation, and so it has been the general consensus that the inherent nature of C_2 in the ground state is experimentally inaccessible. Here, we present the Chemical Synthesis of C_2 from a hypervalent alkynyl-λ^3-iodane in a flask at room temperature or below, providing experimental evidence to support theoretical predictions that C_2 has a singlet biradical character with a quadruple bond, thus settling a long-standing controversy between experimental and theoretical chemists, and that C_2 serves as a molecular element in the bottom-up Chemical Synthesis of nanocarbons such as graphite, carbon nanotubes, and C_60. Diatomic carbon (C_2) is historically an elusive Chemical species, considered to require high physical energy for its generation. Here, the authors describe the first room-temperature Chemical Synthesis of C_2 and present experimental evidence for its singlet biradical (quadruple bonding) character and role as a molecular element of nanocarbons.
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Room-temperature Chemical Synthesis of C 2
Nature communications, 2020Co-Authors: Kazunori Miyamoto, Shodai Narita, Yui Masumoto, Takahiro Hashishin, Taisei Osawa, Mutsumi Kimura, Masahito Ochiai, Masanobu UchiyamaAbstract:Diatomic carbon (C2) is historically an elusive Chemical species. It has long been believed that the generation of C2 requires extremely high physical energy, such as an electric carbon arc or multiple photon excitation, and so it has been the general consensus that the inherent nature of C2 in the ground state is experimentally inaccessible. Here, we present the Chemical Synthesis of C2 from a hypervalent alkynyl-λ3-iodane in a flask at room temperature or below, providing experimental evidence to support theoretical predictions that C2 has a singlet biradical character with a quadruple bond, thus settling a long-standing controversy between experimental and theoretical chemists, and that C2 serves as a molecular element in the bottom-up Chemical Synthesis of nanocarbons such as graphite, carbon nanotubes, and C60.
Kazunori Miyamoto - One of the best experts on this subject based on the ideXlab platform.
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Room-temperature Chemical Synthesis of C_2
Nature Communications, 2020Co-Authors: Kazunori Miyamoto, Shodai Narita, Yui Masumoto, Takahiro Hashishin, Taisei Osawa, Mutsumi Kimura, Masahito Ochiai, Masanobu UchiyamaAbstract:Diatomic carbon (C_2) is historically an elusive Chemical species. It has long been believed that the generation of C_2 requires extremely high physical energy, such as an electric carbon arc or multiple photon excitation, and so it has been the general consensus that the inherent nature of C_2 in the ground state is experimentally inaccessible. Here, we present the Chemical Synthesis of C_2 from a hypervalent alkynyl-λ^3-iodane in a flask at room temperature or below, providing experimental evidence to support theoretical predictions that C_2 has a singlet biradical character with a quadruple bond, thus settling a long-standing controversy between experimental and theoretical chemists, and that C_2 serves as a molecular element in the bottom-up Chemical Synthesis of nanocarbons such as graphite, carbon nanotubes, and C_60. Diatomic carbon (C_2) is historically an elusive Chemical species, considered to require high physical energy for its generation. Here, the authors describe the first room-temperature Chemical Synthesis of C_2 and present experimental evidence for its singlet biradical (quadruple bonding) character and role as a molecular element of nanocarbons.
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Room-temperature Chemical Synthesis of C 2
Nature communications, 2020Co-Authors: Kazunori Miyamoto, Shodai Narita, Yui Masumoto, Takahiro Hashishin, Taisei Osawa, Mutsumi Kimura, Masahito Ochiai, Masanobu UchiyamaAbstract:Diatomic carbon (C2) is historically an elusive Chemical species. It has long been believed that the generation of C2 requires extremely high physical energy, such as an electric carbon arc or multiple photon excitation, and so it has been the general consensus that the inherent nature of C2 in the ground state is experimentally inaccessible. Here, we present the Chemical Synthesis of C2 from a hypervalent alkynyl-λ3-iodane in a flask at room temperature or below, providing experimental evidence to support theoretical predictions that C2 has a singlet biradical character with a quadruple bond, thus settling a long-standing controversy between experimental and theoretical chemists, and that C2 serves as a molecular element in the bottom-up Chemical Synthesis of nanocarbons such as graphite, carbon nanotubes, and C60.
Stephen B. H. Kent - One of the best experts on this subject based on the ideXlab platform.
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Novel protein science enabled by total Chemical Synthesis
Protein science : a publication of the Protein Society, 2018Co-Authors: Stephen B. H. KentAbstract:Chemical Synthesis is a well-established method for the preparation in the research laboratory of multiple-tens-of-milligram amounts of correctly folded, high purity protein molecules. Chemically synthesized proteins enable a broad spectrum of novel protein science. Racemic mixtures consisting of d-protein and l-protein enantiomers facilitate crystallization and determination of protein structures by X-ray diffraction. d-Proteins enable the systematic development of unnatural mirror image protein molecules that bind with high affinity to natural protein targets. The d-protein form of a therapeutic target can also be used to screen natural product libraries to identify novel small molecule leads for drug development. Proteins with novel polypeptide chain topologies including branched, circular, linear-loop, and interpenetrating polypeptide chains can be constructed by Chemical Synthesis. Medicinal chemistry can be applied to optimize the properties of therapeutic protein molecules. Chemical Synthesis has been used to redesign glycoproteins and for the a priori design and construction of covalently constrained novel protein scaffolds not found in nature. Versatile and precise labeling of protein molecules by Chemical Synthesis facilitates effective application of advanced physical methods including multidimensional nuclear magnetic resonance and time-resolved FTIR for the elucidation of protein structure-activity relationships. The chemistries used for total Synthesis of proteins have been adapted to making artificial molecular devices and protein-inspired nanomolecular constructs. Research to develop mirror image life in the laboratory is in its very earliest stages, based on the total Chemical Synthesis of d-protein forms of polymerase enzymes.
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total Chemical Synthesis of proteins
Chemical Society Reviews, 2009Co-Authors: Stephen B. H. KentAbstract:This tutorial review outlines the modern ligation methods that enable the efficient total Chemical Synthesis of enzymes and other protein molecules. Key to this success is the chemoselective reaction of unprotected synthetic peptides (‘Chemical ligation’). Notably, native Chemical ligation enables the reaction of two unprotected peptides in aqueous solution at neutral pH to form a single product in near quantitative yield. Full-length synthetic polypeptides are folded to form the defined tertiary structure of the target protein molecule, which is characterized by mass spectrometry, NMR, and X-ray crystallography, in addition to bioChemical and/or biological activity.
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The Chemical Synthesis of proteins.
Current opinion in biotechnology, 1993Co-Authors: Tom W. Muir, Stephen B. H. KentAbstract:Total Chemical Synthesis is developing as an important approach to the construction of native proteins and their analogs for the study of the structural basis of bioChemical activity. In the past year, a radical departure from conventional synthetic approaches has led to a renaissance in this field. The inherent simplicity of these new modes of protein construction will allow large synthetic proteins (> 200 amino acids in length) to be routinely assembled; this will vastly expand the repertoire of protein molecules accessible to research.
Takahiro Hashishin - One of the best experts on this subject based on the ideXlab platform.
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Room-temperature Chemical Synthesis of C_2
Nature Communications, 2020Co-Authors: Kazunori Miyamoto, Shodai Narita, Yui Masumoto, Takahiro Hashishin, Taisei Osawa, Mutsumi Kimura, Masahito Ochiai, Masanobu UchiyamaAbstract:Diatomic carbon (C_2) is historically an elusive Chemical species. It has long been believed that the generation of C_2 requires extremely high physical energy, such as an electric carbon arc or multiple photon excitation, and so it has been the general consensus that the inherent nature of C_2 in the ground state is experimentally inaccessible. Here, we present the Chemical Synthesis of C_2 from a hypervalent alkynyl-λ^3-iodane in a flask at room temperature or below, providing experimental evidence to support theoretical predictions that C_2 has a singlet biradical character with a quadruple bond, thus settling a long-standing controversy between experimental and theoretical chemists, and that C_2 serves as a molecular element in the bottom-up Chemical Synthesis of nanocarbons such as graphite, carbon nanotubes, and C_60. Diatomic carbon (C_2) is historically an elusive Chemical species, considered to require high physical energy for its generation. Here, the authors describe the first room-temperature Chemical Synthesis of C_2 and present experimental evidence for its singlet biradical (quadruple bonding) character and role as a molecular element of nanocarbons.
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Room-temperature Chemical Synthesis of C 2
Nature communications, 2020Co-Authors: Kazunori Miyamoto, Shodai Narita, Yui Masumoto, Takahiro Hashishin, Taisei Osawa, Mutsumi Kimura, Masahito Ochiai, Masanobu UchiyamaAbstract:Diatomic carbon (C2) is historically an elusive Chemical species. It has long been believed that the generation of C2 requires extremely high physical energy, such as an electric carbon arc or multiple photon excitation, and so it has been the general consensus that the inherent nature of C2 in the ground state is experimentally inaccessible. Here, we present the Chemical Synthesis of C2 from a hypervalent alkynyl-λ3-iodane in a flask at room temperature or below, providing experimental evidence to support theoretical predictions that C2 has a singlet biradical character with a quadruple bond, thus settling a long-standing controversy between experimental and theoretical chemists, and that C2 serves as a molecular element in the bottom-up Chemical Synthesis of nanocarbons such as graphite, carbon nanotubes, and C60.
